RESUMO
The rate of formation of diferric-transferrin has been studied using various combinations of Fe(II), Tf, Cp and h serum. When the reactants were added in a correct physiological sequence, ceruloplasmin and diluted human serum showed the fastest rate of saturation of transferrin.
Assuntos
Ceruloplasmina/metabolismo , Transferrina/metabolismo , Sangue , Humanos , EspectrofotometriaRESUMO
Pharmacokinetic evaluation of Cu(II)2(3,5-diisopropylsalicylate)4(H2O)2 (CuDIPS), a copper complex with anticancer activity in mice, showed rapid absorption into the circulation after subcutaneous (SC) injection of a 0.50mumol, 0.75 mumol or 1.0 mumol dose. A direct relationship was observed between peak plasma copper concentration (one or two hours) and dose. Rate of plasma release also appeared to be dose related. The lowest dose of CuDIPS had no effect on plasma zinc levels; higher doses produced significant increases, but only at one hour. Rapid absorption into the blood (apparent peak in concentration, Tmax, at 0.5 hours) was also found in studies to determine distribution of 67Cu after SC injection of 67Cu and 14C double-labeled CuDIPS (50 mumol/kg body weight). Distribution to other tissues and organs occurred less rapidly with apparent peak 67Cu concentrations at three hours after administration in femur (bone marrow) and intestine, and at six hours in spleen and thymus.
Assuntos
Antineoplásicos/farmacocinética , Salicilatos/farmacocinética , Animais , Cobre/sangue , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Distribuição Tecidual , Zinco/sangueRESUMO
The kinetics of decay in absorbance at 610 nm in the reaction of cysteine with ceruloplasmin was biphasic under anaerobic conditions. Admission of oxygen to the bleached ceruloplasmin restored the blue color to about 75% of the original value. However, under aerobic or anaerobic conditions an initial bleaching corresponded to a 25% decrease in blue color. This change was irreversible and remained after removal of excess cysteine from the reaction mixture by dialysis. There was no correlation between transient and steady-state kinetic parameters. Circular dichroism measurements showed a characteristic reduction in the negative band at 450 nm, which is specific for type 1b copper. Isolation and further studies on cysteine-modified ceruloplasmin with a lower A610/A280 ratio showed less than 10% reduction in enzyme activity toward p-phenylenediamine and o-dianisidine. Evidence is also presented that ceruloplasmin catalyzes the oxidation of cysteine with a one-electron reduction of oxygen and the formation of superoxide ion, which is then converted to H2O2 by ceruloplasmin. The effect of superoxide dismutase and catalase also confirms the presence of superoxide and H2O2. In sum, these data show that a permanent reduction of type 1b copper occurred when cysteine was used as a substrate. We conclude that there is a single electron transfer from cysteine directly to oxygen using one specific copper of ceruloplasmin, type 1b.
Assuntos
Ceruloplasmina/metabolismo , Cobre/metabolismo , Cisteína/metabolismo , Animais , Bovinos , Dicroísmo Circular , Humanos , Peróxido de Hidrogênio/metabolismo , Cinética , Oxirredução , Oxigênio/farmacologia , Espectrofotometria , Superóxidos/metabolismo , SuínosRESUMO
The dietary antagonism between copper and molybdate salts prompted a study of the inhibition of copper enzymes by thiomolybdate (TM). TM strongly inhibited the oxidase activity of five copper oxidase with I50% values in the 1-5 microM range. The mechanism of the TM effect on the copper oxidase, ceruloplasmin (Cp) (E.C. 1.16.3.1), was studied in detail. In Vmax vs. E plots, TM gave parallel data suggesting irreversibility but a large number of TM molecules per Cp were required. The inhibition of Cp by TM could not be reversed by dialysis. Isolation of TM-inhibited Cp on Sephadex G-10 did not yield any active Cp molecules. Cu(II) did not restore any inhibited oxidase activity. Gel electrophoresis supported the covalent binding of Cp by TM without any extensive change in protein structure. EPR results confirmed that Cu(II) is reduced to Cu(I) after reaction with TM. However, the Mo(VI) in MoS4(2-) did not change in oxidation number. Analysis of the TM-Cp compound accounted for all six Cu atoms as found in native Cp. The data suggest the covalent binding of sulfide to Cp copper. TM also inhibited the activity of ascorbate oxidase, cytochrome oxidase, superoxide dismutase, and tyrosinase. However, no inhibition of carbonic anhydrase, a zinc enzyme, was observed at 1 mM TM.
Assuntos
Ceruloplasmina/antagonistas & inibidores , Molibdênio/farmacologia , Oxirredutases/antagonistas & inibidores , Animais , Ascorbato Oxidase/antagonistas & inibidores , Bovinos , Complexo IV da Cadeia de Transporte de Elétrons/antagonistas & inibidores , Humanos , Técnicas In Vitro , Monofenol Mono-Oxigenase/antagonistas & inibidores , Superóxido Dismutase/antagonistas & inibidores , SuínosRESUMO
Several features of the catalytic oxidation of cysteine by ceruloplasmin and nonenzymic Cu(II) at pH 7 have been compared. The oxidation of cysteine by ceruloplasmin has several properties in common with the Cu(II) catalyzed oxidation of cysteine: pH maxima, thiol specificity, lack of inhibition by anions, and high sensitivity to inhibition by copper complexing reagents. These two catalysts differed in their molecular activity, in their ability to oxidize penicillamine and thioglycolate, and in that H2O2 was produced as a primary product only during Cu(II) oxidation. The oxidation of cysteine by ceruloplasmin was compared also with the ceruloplasmin catalyzed oxidation of o-dianisidine, a classical pH 5.5 substrate. The mechanism of the oxidation of cysteine by ceruloplasmin at pH 7 differed from that of o-dianisidine oxidation because the latter substrate was inhibited by anions but not by copper complexing agents. Spectral and other data suggest that during the ceruloplasmin reaction with cysteine there is a one electron transfer from cysteine to ceruloplasmin resulting in the specific reduction of type 1b Cu(II).
Assuntos
Benzidinas/metabolismo , Ceruloplasmina/metabolismo , Cobre/farmacologia , Cisteína/metabolismo , Dianisidina/metabolismo , Animais , Quelantes/farmacologia , Concentração de Íons de Hidrogênio , Cinética , Oxirredução , Especificidade por SubstratoRESUMO
The initial yield of 3,5-di-t-butylsalicylic acid obtained via Kolbe-Schmitt carboxylation of the potassium salt of 2,4-di-t-butylphenol was less than 1% and was accompanied by a 65% yield of 2,2'-dihydroxy-3,3',5,5'- tetra-t-butylbiphenyl, a dimer of the 2,4-di-t-butylphenol formed by ortho coupling of phenoxide radicals. Formation of this dimer was decreased to 8%, and the yield of 3,5-di-t-butylsalicylic acid was increased to 68% by optimizing reaction time and temperature and decreasing the amount of oxygen present during carboxylation. This modification of the Kolbe-Schmitt reaction conditions may be generally helpful in the synthesis of all pharmacologically useful salicylates.
Assuntos
Compostos de Bifenilo/síntese química , Fenóis/síntese química , Salicilatos/síntese química , Oxirredução , Fenóis/química , Salicilatos/química , TemperaturaRESUMO
Copper (II)2(3,5-Diisopropylsalicylate)4(H2O)2 has been found to have antiinflammatory, antiulcer, anticonvulsant, anticancer, anticarcinogenic, antimutagenic, and radiation recovery activities and it prevents reperfusion injury. To study pharmacokinetic parameters accounting for these pharmacological effects the double labeled 67Cu(II)2(carboxy-14C-3,5-diisopropylsalicylate)4 complex was synthesized and used to obtain these parameters. Treatment of mice with 1 mumol of this complex revealed that 67Cu was distributed to blood, liver, kidney, intestine, lung, thymus, femur, muscle, spleen, brain, urine, and feces within 0.5 hr and patterned changes in 67Cu content of these tissues and excreta were found throughout the 96 hr term of this study.
Assuntos
Cobre/uso terapêutico , Animais , Disponibilidade Biológica , Fenômenos Químicos , Química , Doença Crônica , Cobre/farmacocinética , Camundongos , Especificidade de Órgãos , Salicilatos/farmacocinéticaRESUMO
The influence of several commercial albumin preparations on the ferroxidase activity of ceruloplasmin (ferroxidase I, ferrous: O2 oxidoreductase EC 1.16.3.1) at pH 6.0 was determined using ferric-transferrin formation. The ability of several albumin preparations to inhibit the ferroxidase activity of ceruloplasmin differs more than three hundredfold. It appears to depend on the method of isolation of albumin rather than the source of albumin, suggesting the existence of an inhibitor bound to albumin. The inhibitor was isolated after chromatography of an albumin preparation on Sephadex G-200. It was identified as citrate by thin layer chromatography and by comparison of the spectrum of the sulfide-pentabromoacetone derivative. Albumin preparations, even with bound citrate, do not exert a significant inhibitory effect at pH 7.4.
RESUMO
The bioavailability of iron from foods is ultimately determined by interactions between iron and other components in the digestive milieu. Perhaps the most important factor is the concentration of Fe2+ during transit through the duodenum. During in vitro simulations of human digestion it is possible to probe the concentration of Fe2+, the rate of Fe2+ formation, and total iron concentration using ferrous chromogens. It is crucial, of course, that the chromogen not interfere with the redox reactions occurring during digestion. Accordingly, ferrozine was examined with regard to its ability to reduce complexes Fe3+, alter rates of Fe3+ production, detect Fe2+ present in the digestive mixture and differentiate the effects of chelating and reducing agents in the mobilization of iron from pinto beans. The chromogen was found to be free from apparent artefacts and to be a sensitive and reproducible probe of the state of iron in digestive mixtures.
Assuntos
Digestão , Fabaceae/análise , Compostos Ferrosos/metabolismo , Ferrozina/farmacologia , Ferro/farmacocinética , Plantas Medicinais , Triazinas/farmacologia , Disponibilidade Biológica , Quelantes/farmacologia , Compostos Cromogênicos , Humanos , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Oxirredução , Fenantrolinas/farmacologiaRESUMO
Iron deficiency and iron overload affect one billion people worldwide. Treatment of iron malnutrition can be enhanced by an understanding of iron bioavailability from the diet. We have focused on the development of in vitro methods for determining iron bioavailability in the hopes of providing both an understanding of the chemical basis leading to the inhibition or enhancement of iron absorption and the provision of methodologies which will allow nutritionists around the world to ascertain iron bioavailability of local foods and food combinations. The study reported here focuses on the effects of phosvitin, a suspected inhibitor of iron absorption found in egg yolks, on the chemistry of iron during the in vitro enzymatic digestion of pinto beans. Three basic types of information were obtained. First, the total soluble iron was determined during in vitro enzymatic digestion under simulated oral, gastric (pH 2) and duodenal (pH 6) conditions. Phosvitin was found to have a strong solubilizing effect at pH 6 and pH 2 when in the presence of ascorbate. Pyrophosphate also leads to high iron mobilization. A second approach is to determine the static Fe2+ and Fe3+ concentrations following in vitro enzymatic digestion of pinto beans at pH 2 and pH 6. Ascorbic acid enhanced the total soluble iron at both pH values, however, only at pH 2 was a large proportion of the iron found in the Fe2+ state and then only in the presence of phosvitin but not pyrophosphate. A third approach is to determine the amount of Fe2+ formed in the digestive supernatant during a 10-min incubation with ferrozine.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Digestão , Fenômenos Fisiológicos do Sistema Digestório , Proteínas do Ovo/farmacologia , Fabaceae/metabolismo , Ferro/metabolismo , Fosvitina/farmacologia , Plantas Medicinais , Ácido Ascórbico/farmacologia , Disponibilidade Biológica , Fenômenos Químicos , Química , Difosfatos/farmacologia , Duodeno , Humanos , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Ferro/análise , Deficiências de Ferro , Modelos Biológicos , Oxirredução , SolubilidadeRESUMO
The design and use of iron(III) chelates in the oral treatment of iron-deficiency anemia is discussed in terms of the following criteria: (1) the ability of a chelate to exist as a monomeric species even at physiological pH values, (2) the rate of transfer of iron from the chelate to the iron-transporting protein-apotransferrin, (3) the rate of depolymerization of ferric citrate polymer by the free ligand, and (4) its nontoxicity and ability to regenerate hemoglobin levels in anemic rats. Detailed species distribution studies, stability constants, and kinetic data for iron(III) acetohydroxamate (criteria 1-3 above) show that it remains monomeric at physiological pH values and undergoes very rapid iron transfer with apotransferrin. Detailed animal studies show a significant increase in regeneration of hemoglobin in rats fed 2 ml of 4 mM Fe(III) acetohydroxamate daily when compared to rats fed similarly with Fe(III) citrate. A strong potential is thus indicated for Fe(III) acetohydroxamate as a source of iron in the anemic animal.