Systematics and evolutionary dynamics of insect-fern interactions in the specialized fern-spore feeding Cuprininae (Lepidoptera, Stathmopodidae).

Fern-spore-feeding (FSF) is rare and found in only four families of Lepidoptera. Stathmopodidae is the most speciose family that contains FSF species, and its subfamily Cuprininae exclusively specializes on FSF. However, three species of Stathmopodinae also specialize on FSF. To better understand the evolutionary history of FSF and, more generally, the significance of specialization on a peculiar host, a phylogenetic and taxonomic revision for this group is necessary. We reconstructed the most comprehensive molecular phylogeny, including one mitochondrial and four nuclear genes, of Stathmopodidae to date, including 137 samples representing 62 species, with a particular focus on the FSF subfamily, Cuprininae, including 33 species (41% of named species) from 6 of the 7 Cuprininae genera. Species from two other subfamilies, Stathmopodinae and Atkinsoniinae, were also included. We found that FSF evolved only once in Stathmopodidae and that the previous hypothesis of multiple origins of FSF was misled by inadequate taxonomy. Moreover, we showed that (1) speciation/extinction rates do not differ significantly between FSF and non-FSF groups and that (2) oligophage is the ancestral character state in Cuprininae. We further revealed that a faster rate of accumulating specialists over time, and thus a higher number of specialists, was achieved by a higher transition rate from oligophagages to specialists compared to the transition rate in the opposite direction. We finish by describing three new genera, Trigonodagen. nov., Petalagen. nov., and Pediformisgen. nov., and revalidating five genera: Cuprina, Calicotis, Thylacosceles, Actinoscelis, Thylacosceloides in Cuprininae, and we provide an updated taxonomic key to genera and a revised global checklist of Cuprininae.

Optical quantum frequency filter based on generalized eigenstates.

Bound eigenstates and generalized eigenstates (scattering eigenstates) are two kinds of eigenstates in quantum mechanics. In this work, we first introduce a systematic way to regularize a generalized eigenstates by using the Wick rotation. The states that can be regularized are, in fact, Gamow states since they form poles in the scattering matrix but not localized before the Wick rotation. We then demonstrate an example where a bosonic field interacting with an array of two level systems can have Gamow states with positive real eigenenergies, and the scattering spectrum diverges at the eigenenergy. Since the eigenenergies of this kind locate in a real continuous scattering spectrum, from the scattering matrix point of view, these states resemble the bound states in the continuum (BIC). Unlike BIC, however, these states are non-localized in space and possess the frequency-filtering nature which may lead to potential applications in tunable quantum frequency filters for scattering states.

Rapid identification of the invasive fall armyworm Spodoptera frugiperda (Lepidoptera, Noctuidae) using species-specific primers in multiplex PCR.

The fall armyworm (FAW), Spodoptera frugiperda (Smith), is a major pest native to the Americas. A recent invasion of FAWs from Africa eastward to South Asia, the Indochina Peninsula, and mainland China has received much attention due to the considerable economic losses in agriculture. FAWs can rapidly colonise a new area, likely due to the wide range of host plants, good flying capability, and high egg production. Therefore, a convenient, quick, and accurate tool for FAW identification is urgently required to establish a FAW invasion management strategy. In this study, FAW-specific primers were designed to recognise FAWs on the basis of internal transcribed spacer 1 (ITS1). The results revealed the accurate FAW recognition of the three congeneric species and eight common corn lepidopteran pests, especially at their larval stage. Furthermore, species-specific primers have confirmed their efficacy by using 69 FAW specimens from Taiwan, Thailand, and the United States, with a 96% success rate, excluding 3 decayed specimens. By using the simple, reliable, and convenient FAW-specific primers, a pest management programme can be developed not only to reduce sequencing costs and experimental time from 2 days to 4 h, but eradicate the FAW as soon as it enters a new area.

Delineation of two new, highly similar species of Taiwanese Cylindera tiger beetles (Coleoptera, Carabidae, Cicindelinae) based on morphological and molecular evidence.

Tiger beetles have been recognized primarily based on morphological characters. However, the variations of elytral maculation and coloration sometimes lead to misjudgment in species classification and the overlooking of the existence of cryptic species. Recently, specimens of two endemic species of Taiwanese Cylindera, C. sauteri and C. pseudocylindriformis, exhibit morphologically recognizable forms, indicating that some undescribed species may exist. To clarify their taxonomic status, morphological characteristics including male genitalia were examined and two mitochondrial genes, COI and 16S rDNA, and one nuclear 28S rDNA were analyzed. Molecular phylogenetic inferences indicated that both forms in both species are reciprocally monophyletic. Moreover, molecular dating showed the forms diverged approximately 1.3 million years ago. Two new species, Cylindera ooa sp. nov. and Cylindera autumnalis sp. nov., are thereby described. The main recognizable characteristics separating C. ooa sp. nov. from C. sauteri are the lack of a triangular spot at the middle edge of elytron and the elongated but not rounded subapical spot. For C. autumnalis sp. nov., the apical lunula near the elytral suture is thickened but not linear and slender, and its elytra are more metallic brownish than those of C. pseudocylindriformis. Although their aedeagi characteristics are not distinctive, the body size of the proposed two new species is different. Field observation revealed that niche utilization would be relevant for differentiating these closely related species.