The association between season of birth, age at onset, and clozapine use in schizophrenia.

OBJECTIVE: This study aimed to determine whether the rate of clozapine use, an indicator of refractoriness in schizophrenia, is associated with the season of birth and age at onset in patients with schizophrenia based on nationwide data. METHODS: Patients with schizophrenia (n = 114 749) who received prescriptions for antipsychotic medication between 2008 and 2014 were retrospectively identified from the Korean National Health Insurance Service database. The study population was divided into three groups based on their age at the onset of schizophrenia (early, middle, and late onset). We assessed differences in the month of birth between patients and the general population. In addition, the cumulative clozapine use was calculated. RESULTS: Compared to the late-onset schizophrenia group, the early- and middle-onset groups showed a higher probability of birth during the winter season. In addition, the early-onset group showed the highest cumulative clozapine use rate. In the middle-onset group, the initiation of clozapine use was significantly earlier for patients born in winter compared to those born in summer. CONCLUSION: Our results indicate that the age at onset is an important factor in predicting the prognosis of schizophrenia patients. The season of birth also affects the prognosis, but with less robustness. Specifically, it appears that early disease onset and winter birth might be associated with poor outcomes in Korean patients with schizophrenia.

Transmission of 51.56-Gb/s OOK signal using 1.55-µm directly modulated laser and duobinary electrical equalizer.

We demonstrate the transmission of 51.56-Gb/s on-off keying (OOK) signals generated by using a 1.55-µm directly modulated laser (DML) over 15-km long standard single-mode fiber. In this experiment, a duobinary electrical equalizer based on a finite-impulse-response filter is used at the receiver to increase the dispersion-limited transmission distance. We evaluate the performances of the 51.56-Gb/s OOK signals with respect to the transmission distance by using the frequency response analysis of the proposed system. This result is used to explain why it is effective to utilize the duobinary equalization (instead of binary equalization) for increasing the transmission distance.

Anthocyanin contents in the seed coat of black soya bean and their anti-human tyrosinase activity and antioxidative activity.

OBJECTIVE: The seed coat of black soya bean (SCBS) contains high amount of anthocyanins and shows antioxidant and anti-mushroom tyrosinase activities. The objectives of this study were to analyse the anthocyanins in SCBS with different solvents and to find the relationship between anthocyanin profile with anti-human and anti-mushroom tyrosinase activities. METHODS: SCBS was extracted with hot water, 50 and 80% ethanol, 50 and 80% acetone and 50 and 80% acidified acetone. Total phenol and total flavonoid contents in the extracts were determined. Anthocyanins in the extracts were analysed using HPLC and LC/MS/MS. A genetically engineered human tyrosinase was used to evaluate the anti-tyrosinase potential of the extracts from SCBS. RESULTS: 80% acetone extract from SCBS obtained the highest total phenol, total flavonoid and cyanidin-3-O-glucoside (C3G) contents among all the extracts, whereas the hot water extract showed the lowest antioxidant contents. Three anthocyanin compounds were found in all the extracts from SCBS, and the analysis of HPLC and LC/MS/MS indicated that they were C3G, delphinidin-3-O-glucoside (D3G) and peonidin-3-O-glucoside (P3G). The ratios of C3G (2.84 mg g(-1) ), D3G (0.34 mg g(-1) ) and P3G (0.35 mg g(-1) ) in 80% acidified acetone extract were 76.6, 9.1 and 9.3%, respectively. All the extracts from SCBS possessed anti-human tyrosinase activity. Moreover, a good correlation was found between the anti-human tyrosinase activities and C3G contents in the extracts. CONCLUSION: Antioxidants in SCBS also possess anti-human and anti-mushroom tyrosinase activities.

Crosstalk analysis in homogeneous multi-core two-mode fiber under bent condition.

We analyze the inter-core crosstalk in homogeneous multi-core two-mode fibers (MC-TMFs) under bent condition by using the coupled-mode equations. In particular, we investigate the effects of the intra-core mode coupling on the inter-core crosstalk for two different types of MC-TMFs at various bending radii. The results show that the inter-core homo-mode crosstalk of LP(11) mode is dominant under the gentle fiber bending condition due to its large effective area. However, as the fiber bending becomes tight, the intra-core mode coupling is significantly enhanced and consequently makes all the inter-core crosstalk levels comparable to each other regardless of the mode. A similar tendency is observed at a reduced bending radius when the difference in the propagation constants between modes is large and core pitch is small.

Practical 12.5-Gb/s, 12.5-GHz spaced ultra-dense WDM PON.

We report a practical 12.5-Gb/s, 12.5-GHz-spaced ultra-dense wavelength-division-multiplexed passive optical network (UD-WDM PON). For the cost-effectiveness, we implement the downstream links by using electro-absorption modulated lasers (EMLs) in the 4-level pulse amplitude modulation (4PAM) format and PIN receivers, and the upstream links by using reflective semiconductor optical amplifiers (RSOAs) modulated in the quadrature phase-shift-keying (QPSK) format and low-cost self-homodyne receivers. To further enhance its cost-effectiveness, we also utilize an optical frequency comb generator, instead of a large number of wavelength-selected lasers, to provide the seed light for these colorless RSOAs. We optimize the operating conditions of the EMLs and RSOAs to maximize the power margins in the presence of the crosstalk arising from closely spaced neighboring channels and the inter-symbol interference (ISI) caused by the narrow passband of the cascaded arrayed-waveguide gratings (AWGs) as well as the limited modulation bandwidths of RSOAs. The experimental results show that we can secure the power margins of >2.5 dB for both upstream and downstream links of the proposed UD-WDM PON.

Optical performance monitoring technique using software-based synchronous amplitude histograms.

We propose and demonstrate a simple technique to monitor both the optical signal-to-noise ratio (OSNR) and chromatic dispersion (CD) by using the software-based synchronous amplitude histogram (SAH) analysis. We exploit the software-based synchronization technique to construct SAHs from the asynchronously sampled intensities of the signal. The use of SAHs facilitates the accurate extraction of the monitoring parameters at the center of the symbol. Thus, unlike in the case of using the technique based on the asynchronous amplitude histogram (AAH), this technique is not affected by the transient characteristics of the modulated signals. The performance of the proposed monitoring technique is evaluated experimentally by using 10-Gbaud quadrature phase-shift keying (QPSK) and quadrature amplitude modulation (QAM) signals over wide ranges of OSNR and CD. We also evaluate the robustness of the proposed technique to the signal's transient characteristics.

Simple carrier-phase estimator for high-speed RSOA-based coherent WDM PON.

It has been recently reported that reflective semiconductor optical amplifiers (RSOAs) can be used as phase modulators for the cost-effective implementation of the high-speed wavelength-division-multiplexed passive optical networks (WDM PONs). For the detection of the phase-shift-keying (PSK) signal generated by using an RSOA, we should be able to estimate its carrier phase accurately at the digital coherent receiver. However, when the baud rate of this PSK signal is set to be much higher than the RSOA's modulation bandwidth, the conventional M-th power algorithm cannot estimate its carrier phase accurately. To solve this problem, we develop a simple carrier-phase estimation technique for the high-speed (>10 Gb/s) PSK signals generated by using bandwidth-limited RSOAs. This technique estimates the carrier phase of the PSK signal by measuring the angular direction of the opening occurred in the trajectory of the phasor diagram. By using the proposed technique, we demonstrate the upstream transmission of the 25.78-Gb/s quadrature phase-shift-keying (QPSK) signal generated by using an RSOA (3-dB bandwidth: 3.2 GHz) in a 60-km reach coherent WDM PON.

Achievable capacity improvement by using multi-level modulation format in trench-assisted multi-core fiber system.

We evaluate the impacts of using multi-level modulation formats on the transmission capacity of the multi-core fiber (MCF) having trench-assisted index profile and hexagonal layout. For this evaluation, we utilize the spectral efficiency per unit area, defined as the spatial spectral efficiency (SSE). The results show that the SSE improvement achievable by using the higher-level modulation format can be reduced due to its lower tolerance to the inter-core crosstalk. We also evaluate the effects of using large effective area on the transmission capacity of the trench-assisted MCF. The results show that the use of large effective area can decrease this capacity due to the increased inter-core crosstalk and lengthened cable cutoff wavelength, although it can help increase the transmission distance. Thus, it is necessary to optimize the effective area of MCF by considering both the SSE and transmission distance. However, the results indicate that the effect of using different effective areas on the SSE-distance product is not significant, and it is not useful to increase the effective area of the trench-assisted MCF to be larger than ~110 µm(2).

Transmission of 40-Gb/s QPSK upstream signal in RSOA-based coherent WDM PON using offset PDM technique.

We demonstrate the 40-Gb/s upstream transmission in the 60-km reach wavelength-division-multiplexed passive optical network (WDM PON) implemented by using directly modulated reflective semiconductor optical amplifiers (RSOAs) and self-homodyne receivers. It is difficult to operate the RSOA at 40 Gb/s due to its limited modulation bandwidth. To overcome this problem and generate 40-Gb/s upstream signal, we utilize the quadrature phase-shift-keying (QPSK) format and the offset polarization-division-multiplexing (PDM) technique. For this purpose, we install two RSOAs at each ONU and provide the seed light for these RSOAs by polarization-multiplexing the outputs of two lasers with a small frequency offset (20 GHz). This frequency offset is used to separate the polarization-multiplexed seed light by using a simple delay-line interferometer (DLI), instead of the polarization-beam splitter and polarization controller, at the ONU. The separated seed light is modulated by each RSOA at 20 Gb/s in the QPSK format, and then combined again by the DLI before sent back to the central office (CO). The results show that this WDM PON can support the transmission of 40-Gb/s channels spaced at 50 GHz over 60 km without using any remote optical amplifiers.

Correlation-based OTDR for in-service monitoring of 64-split TDM PON.

We demonstrate that the correlation-based optical time domain reflectometer (OTDR) can be used for the in-service monitoring of the 64-split time-division-multiplexed passive optical network (TDM PON). To achieve this objective, we superimpose a pseudo noise (PN) sequence having a modulation depth of ~40% to the downstream signal and utilize it for the correlation detection. However, the use of such a large PN sequence can seriously deteriorate the performance of the downstream receiver. Thus, we apply 8B/10B encoding to the downstream signal, and then filter out the PN sequence at the downstream receiver by using a high-pass filter. As a result, the power penalty caused by the use of a large PN sequence is reduced to an acceptable level (<3 dB), while the dynamic range of this correlation-based OTDR is increased to ~30 dB. We then evaluate the performance of the proposed OTDR in the fiber link similar to the optical distribution network of the 64-split TDM PON. The results show that this OTDR can detect both the reflective and non-reflective events occurred in the feeder fiber as well as the reflective events in the drop fibers even in the 64-split TDM PON.

Long-reach 10-Gb/s RSOA-based WDM PON employing QPSK signal and coherent receiver.

We demonstrate a long-reach wavelength-division-multiplexed passive optical network (WDM PON) operating at the symmetric rate of 10.3 Gb/s. For the cost-effectiveness, we realize the upstream transmission by utilizing directly-modulated TO-can packaged reflective semiconductor optical amplifiers (RSOAs) and digital coherent receivers. In addition, to overcome the limited modulation bandwidth of this TO-can packaged RSOA (~2.2 GHz) and operate it at 10.3 Gb/s, we utilize the quadrature phase shift keying (QPSK) format and the electronic phase equalization technique. The result shows that we can extend the maximum reach of the 10.3-Gb/s RSOA-based WDM PON to ~80 km without using any remote amplifiers.

Large-scale mitochondrial DNA deletions in weak goslings.

1. Evidence has accumulated in mammals to support the idea that mitochondrial DNA (mtDNA) deletions and mutations might contribute to ageing and reproductive failure. White Roman geese were monitored to evaluate the effect of large-scale deletions of mtDNA in an avian species. 2. A total of 340 samples from 114 dead embryos, 111 weak goslings, and 115 normal goslings were used in this experiment. The regions of these two large-scale mtDNA deletions, ΔmtDNA6829 and ΔmtDNA6992, were between the COI and ND5 genes. A 3·6% (4 out of 111) positive sample was detected in the weak goslings. In contrast, no large-scale mitochondrial DNA deletions were detected in either the dead embryos (0 out of 114) or the normal goslings (0 out of 115). 3. Large-scale mtDNA deletions may be a factor causing weak goslings.

Transmission of 1.25-Gb/s PSK signal generated by using RSOA in 110-km coherent WDM PON.

We generate the phase-modulated signal by utilizing the chirp characteristics of the directly-modulated reflective semiconductor optical amplifier (RSOA) for the cost-effective realization of a long-reach wavelength-division-multiplexed passive optical network (WDM PON). We first investigate the relation between the amplitude and phase modulation indices in a directly-modulated RSOA and optimize these modulation indices to maximize the symbol distance on the constellation diagram. The results show that, by operating the RSOA under this optimum condition, we can achieve the excellent receiver sensitivity of -49.8 dBm at 1.25 Gb/s. We implement a long-reach WDM PON by using the phase-modulated RSOAs and self-homodyne receivers, and demonstrate the error-free transmission of 1.25-Gb/s signal over a 110-km long link without using any optical amplifiers.

Phasor monitoring of DxPSK signals using software-based synchronization technique.

We develop a novel phasor monitor to obtain the constellation diagram from asynchronously sampled data measured by using the delay-detection technique. This phasor monitor consists of three parts; a phase-adjustment-free delay-interferometer, an optical front-end made of three photodetectors, and analog-to-digital (A/D) convertors, and a digital signal processor. We operate the A/D convertor at the sampling rate much slower than the symbol rate and acquire the data asynchronously. However, despite the use of such a slow and asynchronous sampling rate, we obtain the clear eye and constellation diagrams by utilizing the software-based synchronization technique based on a novel phased-reference detection algorithm. Thus, the proposed phasor monitor can be implemented without using high-speed A/D convertors and buffer memories, which have been the major obstacles for the cost-effective realization of the phasor monitor. For a demonstration, we realize the proposed phasor monitor by using an A/D converter operating at 9.77 MS/s and used it for the constellation monitoring and bit-error-rate (BER) estimation of 10.7-Gsymbol/s differential quadrature phase-shift keying (DQPSK) and differential 8-ary phase-shift keying (D8PSK) signals.

Microbial community structure and occurrence of diverse autotrophic ammonium oxidizing microorganisms in the anammox process.

The enrichment of anaerobic ammonium oxidizing (anammox) bacteria using an upflow anaerobic sludge bioreactor was successfully conducted for 400 days of continuous operation. The bacterial community structure of anammox bioreactor included Proteobacteria (42%), Chloroflexi (22%), Planctomycetes (20%), Chlorobi (7%), Bacteroidetes (5%), Acidobacteria (2%), and Actinobacteria (2%). All clones of Planctomycetes were affiliated with the anammox bacteria, Planctomycete KSU-1 (AB057453). The presence and diversity of ammonia oxidizing bacteria (AOB) and archaea (AOA) were identified by terminal restriction fragment length polymorphism (T-RFLP) based on the amoA gene sequences. The AOB in anammox bioreactor were affiliated with the Nitrosomonas europaea cluster. The T-RFLP result of AOA showed the diverse microbial community structure of AOA with three terminal restriction fragments (T-RFs).

Magnetic glassy state at low spin state of Co3+ in EuBaCo2O5+δ (δ = 0.47) cobaltite.

The magnetic glassy state is a fascinating phenomenon, which results from the kinetic arrest of the first order magnetic phase transition. Interesting properties, such as metastable magnetization and nonequilibrium magnetic phases, are naturally developed in the magnetic glassy state. Here, we report magnetic glass property in the low spin state of Co3+ in EuBaCo2O5+δ (δ = 0.47) cobaltite at low temperature (T < 60 K). The measurements of magnetization under the cooling and heating in unequal fields, magnetization relaxation and thermal cycling of magnetization show the kinetic arrest of low magnetization state below 60 K. The kinetically arrested low temperature magnetic phase is further supported through the study of isothermal magnetic entropy, which shows the significant entropy change. The present results will open a new window to search the microscopic relation between the spin state transitions and the kinetic arrest induced magnetic glassy phenomena in complex materials.

Optical performance monitoring technique using asynchronous amplitude and phase histograms.

We report a new optical performance monitoring technique based on the amplitude and phase histograms of the optical signal. The amplitude and phase histograms are obtained by using an intradyne receiver and the asynchronous delay-tap sampling method. From these histograms, we can evaluate the amplitude and phase Q-factors of the optical signal regardless of its modulation format. In addition, by using these Q-factors, we can estimate the bit-error rate (BER) of the monitoring signal.

Transmission of 108-Gb/s PDM 16ADPSK signal on 25-GHz grid using non-coherent receivers.

We demonstrate the transmission of 108-Gb/s polarization-division-multiplexed (PDM) 16-ary amplitude- and differential phase-shift-keying (16ADPSK) signal by using a non-coherent receiver. We generate the 16ADPSK signal by using a differential 8-ary phase-shift-keying (D8PSK) modulator and a phase-distortion-free amplitude-shift-keying (ASK) modulator. On the other hand, the receiver is implemented by using a delay interferometer based on a 3x3 fiber coupler and the data-aided phase-noise estimation (DAPNE) algorithm. By using these transmitter and receiver, we achieve a nearly quantum-limited receiver sensitivity in the back-to-back condition. In addition, we examine the possibility of transmitting 108-Gb/s signals on a 25-GHz grid without using the coherent detection technology. The results show that we can secure a sufficient optical-signal-to-noise (OSNR) margin after the transmission of 80-km long dispersion managed link. The achieved spectral efficiency is 4.0 bit/s/Hz.

Effect of PDL-induced coherent crosstalk on polarization-division-multiplexed direct-detection systems.

We analyze the effect of polarization-dependent loss (PDL) on the performance of the polarization-division-multiplexed (PDM) signals in direct-detection systems. We present the theoretical analysis of the sensitivity penalty caused by PDL-induced coherent crosstalk and compare the results with experimental data obtained by using a simple variable PDL emulator based on a LiNbO3 Mach-Zehnder modulator. Also presented is the performance degradation caused by the polarization misalignment between the received signals and the polarization beam splitter at the receiver in the presence of PDL.