RESUMO
Circling behavior in animals lesioned unilaterally in one striatum with 6-hydroxydopamine is widely used to test pharmacological compounds with dopaminergic activity. Although automated techniques employed to record this behavior have previously been reported, most of these methods either limit the movement of the animal or are less reliable for various reasons. This paper describes a new method which allows the free movement of the animal in addition to recording the circling behavior automatically. Furthermore, this device provides new and unique data on the diameter and total area of the circles that are circumscribed by the animal. Consequently, the present device may prove to be a better and more reliable tool to assess the pharmacological effect of drugs on the striatal dopaminergic system.
Assuntos
Atividade Motora , Comportamento Estereotipado , Anfetamina/farmacologia , Animais , Autoanálise , Computadores , Humanos , Hidroxidopaminas/farmacologia , Masculino , Métodos , Camundongos , Atividade Motora/efeitos dos fármacos , Oxidopamina , Rotação , Comportamento Estereotipado/efeitos dos fármacosRESUMO
A simple method has been developed for continuous monitoring of metabolic activity of an isolated, perfused rat heart by O2/CO2 respirometer. Since respirometer provides vital data on oxygen consumption and carbon dioxide production of a preserved organ on a continuous basis over a long period of time, it will be possible to use this method to monitor viability of not only isolated heart but also any given donor organ under preservation.
Assuntos
Coração/fisiologia , Perfusão/métodos , Ar , Animais , Dióxido de Carbono/metabolismo , Técnicas In Vitro , Miocárdio/metabolismo , Consumo de Oxigênio/fisiologia , RatosRESUMO
Traditional methods for measuring cardiac output in mice are invasive and traumatic. The authors discuss using the less-invasive thermodilution method, which is widely accepted in humans and other animals.