Growth, biochemical, and antioxidant response of pot marigold (Calendula officinalis L.) grown in fly ash amended soil.

The present study was carried out to determine the impact of FA application on growth performance, biochemical parameters, and antioxidant defense activity of Calendula officinalis. The results revealed that under a low dose of FA (40%) amended soil, the plant growth performance and metal tolerance index (MTI) were increased compared to control plants and further decreased with increased FA application (60%, 80%, and 100% FA). In addition, the incorporation of 40% FA in soil not only improved the physicochemical properties of soil but also increased the biochemical parameters in the Calendula plant, however, these parameters declined under high FA applications. It was also observed that antioxidant enzyme activity (SOD, CAT, POD, and APX) in leaves of Calendula officinalis increased at high FA application (100% FA) to combat heavy metal stress from FA. The overall study suggests that 40% FA amended soil is the best suitable dose for growing Calendula officinalis and can be considered as metal tolerant species for phytoremediation of 40% FA amended soil.Novelty statement: Fly ash (FA) management is a major problem nowadays. The present study was carried out for FA utilization and to determine the impact of FA amended soil on growth performance, antioxidant properties, and biochemical attributes of Calendula officinalis. This is a sustainable approach in which waste (FA) utilization was done simultaneously with the enhancement in response of the medicinally potent Calendula species. The novelty of this study also suggests that Calendula has phytoremediation potential for remediation of heavy metal polluted soil. Further, the relationship between the growth, biochemical parameters, and antioxidant defense mechanism of Calendula grown on FA amended soil was studied which has not been studied so far. It was found that Calendula is a hyperaccumulator that can adapt to heavy metal stress from FA due to its ability to mitigate oxidative damage. Statistical analysis (ANOVA, Duncan's multiple range test, and PCA) was done for the results obtained using SPSS (11.5) and Origin 8 Pro software.

Navigating the Post-COVID-19 Immunological Era: Understanding Long COVID-19 and Immune Response.

The COVID-19 pandemic has affected the world unprecedentedly, with both positive and negative impacts. COVID-19 significantly impacted the immune system, and understanding the immunological consequences of COVID-19 is essential for developing effective treatment strategies. The purpose of this review is to comprehensively explore and provide insights into the immunological aspects of long COVID-19, a phenomenon where individuals continue to experience a range of symptoms and complications, even after the acute phase of COVID-19 infection has subsided. The immune system responds to the initial infection by producing various immune cells and molecules, including antibodies, T cells, and cytokines. However, in some patients, this immune response becomes dysregulated, leading to chronic inflammation and persistent symptoms. Long COVID-19 encompasses diverse persistent symptoms affecting multiple organ systems, including the respiratory, cardiovascular, neurological, and gastrointestinal systems. In the post-COVID-19 immunological era, long COVID-19 and its impact on immune response have become a significant concern. Post-COVID-19 immune pathology, including autoimmunity and immune-mediated disorders, has also been reported in some patients. This review provides an overview of the current understanding of long COVID-19, its relationship to immunological responses, and the impact of post-COVID-19 immune pathology on patient outcomes. Additionally, the review addresses the current and potential treatments for long COVID-19, including immunomodulatory therapies, rehabilitation programs, and mental health support, all of which aim to improve the quality of life for individuals with long COVID-19. Understanding the complex interplay between the immune system and long COVID-19 is crucial for developing targeted therapeutic strategies and providing optimal care in the post-COVID-19 era.

An innovative approach of bioremediation in enzymatic degradation of xenobiotics.

Worldwide, environmental pollution due to a complex mixture of xenobiotics has become a serious concern. Several xenobiotic compounds cause environmental contamination due to their severe toxicity, prolonged exposure, and limited biodegradability. From the past few decades, microbial-assisted degradation (bioremediation) of xenobiotic pollutants has evolved as the most effective, eco-friendly, and valuable approach. Microorganisms have unique metabolism, the capability of genetic modification, diversity of enzymes, and various degradation pathways necessary for the bioremediation process. Microbial xenobiotic degradation is effective but a slow process that limits its application in bioremediation. However, the study of microbial enzymes for bioremediation is gaining global importance. Microbial enzymes have a huge ability to transform contaminants into non-toxic forms and thereby reduce environmental pollution. Recently, various advanced techniques, including metagenomics, proteomics, transcriptomics, metabolomics are effectively utilized for the characterization, metabolic machinery, new proteins, metabolic genes of microorganisms involved in the degradation process. These advanced molecular techniques provide a thorough understanding of the structural and functional aspects of complex microorganisms. This review gives a brief note on xenobiotics and their impact on the environment. Particular attention will be devoted to the class of pollutants and the enzymes such as cytochrome P450, dehydrogenase, laccase, hydrolase, protease, lipase, etc. capable of converting these pollutants into innocuous products. This review attempts to deliver knowledge on the role of various enzymes in the biodegradation of xenobiotic pollutants, along with the use of advanced technologies like recombinant DNA technology and Omics approaches to make the process more robust and effective.

Synthesis of Alginate Nanogels with Polyvalent 3D Transition Metal Cations: Applications in Urease Immobilization.

Biocompatible nanogels are highly in demand and have the potential to be used in various applications, e.g., for the encapsulation of sensitive biomacromolecules. In the present study, we have developed water-in-oil microemulsions of sodium alginate sol/hexane/Span 20 as a template for controlled synthesis of alginate nanogels, cross-linked with 3d transition metal cations (Mn2+, Fe3+, and Co2+). The results suggest that the stable template of 110 nm dimensions can be obtained by microemulsion technique using Span 20 at concentrations of 10mM and above, showing a zeta potential of -57.3 mV. A comparison of the effects of the cross-links on the morphology, surface charge, protein (urease enzyme) encapsulation properties, and stability of the resulting nanogels were studied. Alginate nanogels, cross-linked with Mn2+, Fe3+, or Co2+ did not show any gradation in the hydrodynamic diameter. The shape of alginate nanogels, cross-linked with Mn2+ or Co2+, were spherical; whereas, nanogels cross-linked with Fe3+ (Fe-alginate) were non-spherical and rice-shaped. The zeta potential, enzyme loading efficiency, and enzyme activity of Fe-alginate was the highest among all the nanogels studied. It was found that the morphology of particles influenced the percent immobilization, loading capacity, and loading efficiency of encapsulated enzymes. These particles are promising candidates for biosensing and efficient drug delivery due to their relatively high loading capacity, biocompatibility, easy fabrication, and easy handling.

Characterization of an extracellular alkaline lipase from Pseudomonas mendocina M-37.

A strain of Pseudomonas mendocina producing extracellular lipase was isolated from soil. The bacterium accumulates lipase in culture fluid when grown aerobically at 30 °C for 24 h in a medium composed of olive oil (1%) as substrate. Pseudomonas mendocina lipase was optimally active at pH 9.0, temperature of 50 °C and was found to be stable between pH 7.0-11.0. The lipase was inhibited by detergents such as SDS and Tween-80. The enzyme was stable in various organic solvents tested with maximum stability in chloroform followed by toluene and exhibited 1-3 regiospecificity for hydrolytic reaction. This lipase was capable of hydrolyzing a variety of lipidic substrates and is mainly active towards synthetic triglycerides and fatty acid esters that possess a butyryl group. Metal ions like Mg(2+), Ca(2+) and Na(+) stimulated lipase activity, whereas, Cu(2+), Mn(2+) and Hg(2+) ions caused inhibition.

Knowledge of, attitude towards, and prevalence of tobacco use among dental students in Himachal Pradesh State, India.

AIM: The aim of the study was to assess the prevalence of tobacco use and knowledge of and attitude towards cessation counselling among dental students in Himachal Pradesh State, India. METHOD: The present study was conducted with the 219 third-year Bachelor of Dental Surgery (BDS) students in the five dental colleges of Himachal Pradesh during the month of March 2012. The Global Health Professional Students Survey (GHPSS) questionnaire, developed by the World Health Organization (WHO), the US Centers for Disease Control and Prevention (CDC) and the Canadian Public Health Association, was used in this study. The data were evaluated using statistical software and statistically tested with the chi-square test. RESULTS: The response was 210 (96%). Lifetime prevalence was 15% for smoking and 2% for tobacco use other than for smoking. Eighteen (9%) students were current smokers and four (2%) were tobacco users other than for smoking. Male students were significantly (P<0.001) more likely than female students to be current cigarette smokers. Approximately 14 (47%) of sometime smokers had smoked on school property and two (50%) of the sometime users of other tobacco products had used those products on school property. As for attitudes, 169 (85%) felt that health professionals serve as role models for their patients and the public. Only 17 (8.5%) of surveyed students had received formal instruction in smoking-cessation approaches during their training and 167 (84.3%) thought that health professionals should receive specific training on cessation techniques. CONCLUSION: Although the current prevalence of tobacco use among third-year dental students in Himachal Pradesh is low, it still needs to be addressed by providing them with the necessary skills and support to quit smoking successfully and to provide counselling.

Phytochemical Screening and Antimicrobial Activity of Some Medicinal Plants Against Multi-drug Resistant Bacteria from Clinical Isolates.

The in vitro antibacterial activity of various solvents and water extracts of aloe vera, neem, bryophyllum, lemongrass, tulsi, oregano, rosemary and thyme was assessed on 10 multi-drug resistant clinical isolates from both Gram-positive and Gram-negative bacteria and two standard strains including Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The zone of inhibition as determined by agar well diffusion method varied with the plant extract, the solvent used for extraction, and the organism tested. Klebsiella pneumoniae 2, Escherichia coli 3 and Staphylococcus aureus 3 were resistant to the plant extracts tested. Moreover, water extracts did not restrain the growth of any tested bacteria. Ethanol and methanol extracts were found to be more potent being capable of exerting significant inhibitory activities against majority of the bacteria investigated. Staphylococcus aureus 1 was the most inhibited bacterial isolate with 24 extracts (60%) inhibiting its growth whereas Escherichia coli 2 exhibited strong resistance being inhibited by only 11 extracts (28%). The results obtained in the agar diffusion plates were in fair correlation with that obtained in the minimum inhibitory concentration tests. The minimum inhibitory concentration of tulsi, oregano, rosemary and aloe vera extracts was found in the range of 1.56-6.25 mg/ml for the multi-drug resistant Staphylococcus aureus isolates tested whereas higher values (6.25-25 mg/ml) were obtained against the multi-drug resistant isolates Klebsiella pneumoniae 1 and Escherichia coli 1 and 2. Qualitative phytochemical analysis demonstrated the presence of tannins and saponins in all plants tested. Thin layer chromatography and bioautography agar overlay assay of ethanol extracts of neem, tulsi and aloe vera indicated flavonoids and tannins as major active compounds against methicillin-resistant Staphylococcus aureus.