The Effects of Shrub Removal on Soil Microbial Communities in Primary Forest, Secondary Forest and Plantation Forest on Changbai Mountain.

Shrub removal is a common management method in forest ecosystems, but comparatively little is known regarding the effects of shrub removal on soil microbial communities among primary forest, secondary forest, and plantation forests in temperate forests, which limits our accurate assessment of sustainable management of understory vegetation removal. Given this, we used a long-term operation experiment across a contrasting mixed broadleaved-Pinus koraiensis forest, Betula platyphylla forest, and Larix gmelinii plantation forest to explore the variations of soil properties and microbial community after 5 years of shrub removal on Changbai Mountain, as well as the contribution of the soil properties and understory plant diversity to the soil microbial community. The results demonstrated that shrub removal could significantly alter soil SWC and TN, TP, and AP contents of the L. gmelinii, as well as N/P of B. platyphylla. Moreover, shrub removal also clearly improved soil bacterial Pielou_e index and Simpson index of mixed broadleaved-P. koraiensis and soil bacterial Simpson index of L. gmelinii, and decreased soil fungal Pielou_e index and Shannon index of L. gmelinii and soil bacterial Pielou_e index and soil fungal Shannon index of B. platyphylla. Identically, shrub removal notably altered the soil bacterial community composition. Soil characteristics and understory plant diversity accounted for 48.02% and 26.88%, and 45.88% and 27.57% of the variance in the bacterial and fungal community composition, respectively. This study aimed to provide an important scientific basis for the restoration and sustainable management of temperate forests in the Changbai Mountain region.

Characterization of Colletotrichum Causing Anthracnose on Rubber Trees in Yunnan: Two New Records and Two New Species from China.

Among the most damaging diseases of rubber trees is anthracnose caused by the genus Colletotrichum, which leads to significant economic losses. Nonetheless, the specific Colletotrichum spp. that infect rubber trees in Yunnan Province, an important natural rubber base in China, have not been extensively investigated. Here, we isolated 118 Colletotrichum strains from rubber tree leaves exhibiting anthracnose symptoms in multiple plantations in Yunnan. Based on comparisons of their phenotypic characteristics and internal transcribed spacer ribosomal DNA sequences, 80 representative strains were chosen for additional phylogenetic analysis based on eight loci (act, ApMat, cal, CHS-1, GAPDH, GS, his3, and tub2), and nine species were identified. Colletotrichum fructicola, C. siamense, and C. wanningense were found to be the dominant pathogens causing rubber tree anthracnose in Yunnan. C. karstii was common, whereas C. bannaense, C. brevisporum, C. jinpingense, C. mengdingense, and C. plurivorum were rare. Among these nine species, C. brevisporum and C. plurivorum are reported for the first time in China, and two species are new to the world: C. mengdingense sp. nov. in the C. acutatum species complex and C. jinpingense sp. nov. in the C. gloeosporioides species complex. Their pathogenicity was confirmed with Koch's postulates by inoculating each species in vivo on rubber tree leaves. This study clarifies the geographic distribution of Colletotrichum spp. associated with anthracnose on rubber trees in representative locations of Yunnan, which is crucial for the implementation of quarantine measures.

miR-6769b-5p targets CCND-1 to regulate proliferation in cadmium-treated placental trophoblasts: Association with the impairment of fetal growth.

Environmental cadmium (Cd) is positively associated with placental impairment and fetal growth retardation. Nevertheless, its potential mechanisms remain unclear. microRNAs (miRNAs) are known to influence placental development and fetal growth. This work was aimed to determine which miRNAs are involved in Cd-impaired placental and fetal development based on the mRNA and miRNA expression profiles analysis. As a result, gestational Cd exposure deceased fetal and placental weight, and reduced the protein level of PCNA in human and mouse placentae. Furthermore, the results of mRNA microarray showed that Cd-downregulated mRNAs were predictively correlated with several biological processes, including cell proliferation, differentiation and motility. In addition, the results of miRNA microarray and qPCR assay demonstrated that Cd significantly increased the level of miR-6769b-5p, miR-146b-5p and miR-452-5p. Integrated analysis of Cd-upregulated miRNAs predicted target genes and Cd-downregulated mRNAs found that overlapping mRNAs, such as CCND1, CDK13, RINT1 and CDC26 were also significantly associated with cell proliferation. Further experiments showed that miR-6769b-5p inhibitor, but not miR-146b-5p and miR-452-5p, markedly reversed Cd-downregulated the expression of proliferation-related mRNAs, and thereby restored Cd-decreased the proteins level of CCND1 and PCNA in human placental trophoblasts. Dual luciferase reporter assay further revealed that miR-6769b-5p directly targets CCND1. Finally, the case-control study demonstrated that increased miR-6769b-5p level and impaired cell proliferation were observed in small-for-gestational-age human placentae. In conclusion, miR-6769b-5p targets CCND-1 to regulate proliferation in Cd-treated placental trophoblasts, which is associated with the impairment of fetal growth. Our findings imply that placental miR-6769b-5p may be used as an epigenetic marker for environmental pollutants-caused fetal growth restriction and its late-onset chronic diseases.

ORAI1-Regulated Gene Expression in Breast Cancer Cells: Roles for STIM1 Binding, Calcium Influx and Transcription Factor Translocation.

A remodeling of calcium homeostasis, including calcium influx via store-operated calcium entry (SOCE), is a feature of breast cancers. SOCE is critical to maintain calcium balance in the endoplasmic reticulum calcium store and is an important mechanism for calcium signaling in a variety of cell types, including breast cancer cells. The canonical mechanism of SOCE is stromal interacting molecule 1 (STIM1)-mediated activation of ORAI. Elevated ORAI1 expression is a feature of basal breast cancer cells. However, the role of ORAI1 in the regulation of transcription in breast cancer cells of the basal molecular subtype is still unclear. Using CRISPR-Cas9 gene editing, ORAI1 protein expression was disrupted in MDA-MB-231 and MDA-MB-468 basal breast cancer cells. The ORAI1 wild-type and mutants were reintroduced into ORAI1 knockout cells to study the role of ORAI1 in gene transcriptional regulation. In the absence of calcium store depletion, ORAI1 regulated PTGS2 in MDA-MB-231 cells, and this was dependent on ORAI1 pore function and STIM1 binding. The activation of SOCE by thapsigargin resulted in ORAI1-dependent increases in IL6 transcription in MDA-MB-468 cells; this was also dependent on ORAI1 pore function and STIM1 binding and was associated with the translocation of NFAT1. Given the upregulation of ORAI1 in basal breast cancer cells, our results provide further evidence that ORAI1 may contribute to cancer progression through regulation of gene expression.

A framework of freshwater services flow model into assessment on water security and quantification of transboundary flow: A case study in northeast China.

Ecosystem service flow dynamics which establish the linkage between human and nature is essential in an ecosystem service assessment. This study constructed an ecosystem service flow model of freshwater flow then utilized it to assess the water-related ecosystem services in northeast China. We included the provision, consumption, and spatial flow of freshwater services in an index to assess the water security condition and quantified the services trans-boundary flow from the northeast forest belt (NFB) in northeast China. Our results showed that large areas (50.54%, 55.10% and 52.90%, respectively) of northeast China received upstream freshwater service in three years. The water security condition of northeast China deteriorated from 2005 to 2015 with the change of water security index considering water flow (WSIflow), mainly influenced by precipitation and agriculture water consumption. Approximately 4.16 billion m3 of freshwater service were delivered from NFB to surrounding regions demonstrating the importance of NFB in terms of ecosystem service provision. In addition, 73 key watersheds (4.71% of total area) within NFB that significantly affect the trans-boundary flow were further identified. We suggested that local government should advocate develop water-saving agriculture and livestock water quotas. Moreover, priorities should be given to protect the key watersheds within NFB in order to maintain the supply of freshwater service. This study provided a framework for exploring suitable strategies for managing water resources and laid a foundation for promoting the ecological compensation in the future.

Effects of national ecological restoration projects on carbon sequestration in China from 2001 to 2010.

The long-term stressful utilization of forests and grasslands has led to ecosystem degradation and C loss. Since the late 1970s China has launched six key national ecological restoration projects to protect its environment and restore degraded ecosystems. Here, we conducted a large-scale field investigation and a literature survey of biomass and soil C in China's forest, shrubland, and grassland ecosystems across the regions where the six projects were implemented (∼16% of the country's land area). We investigated the changes in the C stocks of these ecosystems to evaluate the contributions of the projects to the country's C sink between 2001 and 2010. Over this decade, we estimated that the total annual C sink in the project region was 132 Tg C per y (1 Tg = 1012 g), over half of which (74 Tg C per y, 56%) was attributed to the implementation of the projects. Our results demonstrate that these restoration projects have substantially contributed to CO2 mitigation in China.

Gestational cadmium exposure impairs placental angiogenesis via activating GC/GR signaling.

Gestational exposure to environmental Cd caused placental angiogenesis impairment and fetal growth restriction (FGR). However, its mechanism remained unclear. This study was to investigate the effects of Cd exposure during pregnancy on placental angiogenesis and its mechanism. Pregnant mice were exposed to CdCl2 (4.5 mg/kg) on gestational day (GD) 8 with or without melatonin (MT) (5.0 mg/kg), an anti-endoplasmic reticulum stress agent, from GD7 to GD15. Human primary placental trophoblasts and JEG-3 cells were stimulated using CdCl2 (20 µM) after MT (1 mM) preprocessing. We firstly found MT treatment obviously mitigated environmental Cd-induced placental angiogenesis disorder and reduction of the VEGF-A level. Mechanistically, MT reversed environmental Cd-downregulated the protein expression of VEGF-A via inhibiting glucocorticoid receptor (GR) activation. Notably, our data showed MT treatment antagonized Cd-activated GC/GR signaling via blocking PERK signaling and thereby upregulated VEGF-A and 11ß-HSD2 protein expression. Based upon the population case-control study, the levels of VEGF-A and 11ß-HSD2 protein in small-for-gestational-age placentae were significantly reduced when compared to appropriate-for-gestational-age placentae. Overall, environmental Cd exposure during gestation impaired placental angiogenesis via PERK-regulated GC/GR signaling in placental trophoblasts. Our findings will provide a basis for prevention and treatment of placental impairments and fetal growth restriction caused by environment toxicants in future.

Classification of Frozen Corn Seeds Using Hyperspectral VIS/NIR Reflectence Imaging.

A VIS/NIR hyperspectral imaging system was used to classify three different degrees of freeze-damage in corn seeds. Using image processing methods, the hyperspectral image of the corn seed embryo was obtained first. To find a relatively better method for later imaging visualization, four different pretreatment methods (no pretreatment, multiplicative scatter correction (MSC), standard normal variation (SNV) and 5 points and 3 times smoothing (5-3 smoothing)), four wavelength selection algorithms (successive projection algorithm (SPA), principal component analysis (PCA), X-loading and full-band method) and three different classification modeling methods (partial least squares-discriminant analysis (PLS-DA), K-nearest neighbor (KNN) and support vector machine (SVM)) were applied to make a comparison. Next, the visualization images according to a mean spectrum to mean spectrum (M2M) and a mean spectrum to pixel spectrum (M2P) were compared in order to better represent the freeze damage to the seed embryos. It was concluded that the 5-3 smoothing method and SPA wavelength selection method applied to the modeling can improve the signal-to-noise ratio, classification accuracy of the model (more than 90%). The final classification results of the method M2P were better than the method M2M, which had fewer numbers of misclassified corn seed samples and the samples could be visualized well.

Carbon dynamics in the deciduous broadleaf tree Erman's birch (Betula ermanii) at the subalpine treeline on Changbai Mountain, Northeast China.

PREMISE OF THE STUDY: The growth limitation hypothesis (GLH) and carbon limitation hypothesis (CLH) are two dominant explanations for treeline formation. The GLH proposes that low temperature drives the treeline through constraining C sinks more than C sources, and it predicts that non-structural carbohydrate (NSC) levels are static or increase with elevation. Although the GLH has received strong support globally for evergreen treelines, there is still no consensus for deciduous treelines, which experience great asynchrony between supply and demand throughout the year. METHODS: We investigated growth and the growing-season C dynamics in a common deciduous species, Erman's birch (Betula ermanii), along an elevational gradient from the closed forest to the treeline on Changbai Mountain, Northeast China. Samples were collected from developing organs (leaves and twigs) and main storage organs (stems and roots) for NSC analysis. KEY RESULTS: Tree growth decreased with increasing elevation, and NSC concentrations differed significantly among elevations, organs, and sampling times. In particular, NSC levels varied slightly during the growing season in leaves, peaked in the middle of the growing season in twigs and stems, and increased continuously throughout the growing season in roots. NSCs also tended to increase or vary slightly in developing organs but decreased significantly in mature organs with increasing elevation. CONCLUSIONS: The decrease in NSCs with elevation in main storage organs indicates support for the CLH, while the increasing or static trends in new developing organs indicate support for the GLH. Our results suggest that the growth limitation theory may be less applicable to deciduous species' growth than to that of evergreen species.

Recent advances in starch-based coatings for the postharvest preservation of fruits and vegetables.

Efficient and low-cost postharvest preservation of fruits and vegetables has always been one of the urgent problems to be solved in the food field. Due to the wide sources, good environmental and human safety, and high biodegradability, starch-based coating preservation method has great application prospects in the postharvest preservation of fruits and vegetables. However, starch materials also have the disadvantages of poor mechanical properties and easy water absorption performance, which makes it difficult to fully meet the requirements in practical production. Therefore, starch is often used in combination with other components to form composite materials. This paper began with an introduction to the preservation principles of edible starch-based coatings, including inherent properties and extra functional properties. Besides, the preservation principles of edible coatings and the recent advances in the field of fruit and vegetable preservation were also comprehensively reviewed, focusing on the preparation and application of starch-based coatings. The information will contribute to the further development of starch-based coatings to improve the postharvest preservation effect of fruits and vegetables.

An application of remote sensing data in mapping landscape-level forest biomass for monitoring the effectiveness of forest policies in northeastern China.

Monitoring the dynamics of forest biomass at various spatial scales is important for better understanding the terrestrial carbon cycle as well as improving the effectiveness of forest policies and forest management activities. In this article, field data and Landsat image data acquired in 1999 and 2007 were utilized to quantify spatiotemporal changes of forest biomass for Dongsheng Forestry Farm in Changbai Mountain region of northeastern China. We found that Landsat TM band 4 and Difference Vegetation Index with a 3 × 3 window size were the best predictors associated with forest biomass estimations in the study area. The inverse regression model with Landsat TM band 4 predictor was found to be the best model. The total forest biomass in the study area decreased slightly from 2.77 × 10(6) Mg in 1999 to 2.73 × 10(6) Mg in 2007, which agreed closely with field-based model estimates. The area of forested land increased from 17.9 × 10(3) ha in 1999 to 18.1 × 10(3) ha in 2007. The stabilization of forest biomass and the slight increase of forested land occurred in the period following implementations of national forest policies in China in 1999. The pattern of changes in both forest biomass and biomass density was altered due to different management regimes adopted in light of those policies. This study reveals the usefulness of the remote sensing-based approach for detecting and monitoring quantitative changes in forest biomass at a landscape scale.

Recent preparation, modification and application progress of starch nanocrystals: A review.

Due to the advantages of wide sources, high biocompatibility and favorable biodegradability, starch nanocrystals (SNCs) have gradually attracted attention and have bright development prospects in food, agriculture, materials, medicine and other fields. However, the traditional preparation method of SNCs is time-consuming and inefficient, and the physicochemical properties cannot fully meet the needs of multiple applications. Fortunately, the unique onion-like structure of starch granules and the large number of hydroxyl groups present on the surface entitle SNCs to efficient preparation and modification. This paper comprehensively reviewed the improvement methods of SNCs preparation process in recent years, and the advantages and disadvantages of the two improvement strategies were compared. Besides, the importance of introducing different pretreatment methods into the SNCs preparation process was emphasized. It also focused on the different modification treatment and application progress of SNCs, especially in the starch-based surface coating of fruits and vegetables. The information will contribute to further improve the preparation efficiency and physicochemical properties of SNCs, and ultimately expand the application field.

Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice.

Cadmium (Cd) is a well-known testicular toxicant. Blood-testis barrier (BTB), a vital part of testes, which has been reported to be damaged upon Cd exposure. However, the detailed mechanism about Cd-mediated disruption of BTB remains unclear. This study aims to investigate the role of Heme-Regulated Inhibitor (HRI)-responsive mitochondrial stress in Cd-mediated disruption of BTB. Male mice are intraperitoneally injected (i.p.) with melatonin (Mel, a cellular stress antagonist, 5.0 mg/kg) before Cd treatment (i.p., 2.0 mg/kg) for 8 h, and then treated with Cd for 0-48 h. Mouse Sertoli cells are pretreated with Mel (10 µM) for 1 h, and then treated with Cd (10 µM) for 0-24 h. We find that Cd damages the BTB and reduces the Occludin protein, a crucial BTB-related protein via activating p38/matrix metalloproteinase-2 (p38/MMP2) pathway and Integrated Stress Response (ISR). Further experiments reveal that the Heme-Regulated Inhibitor (HRI)-responsive mitochondrial stress is triggered in Cd-treated Sertoli cells. Most importantly, Cd-activated p38 signaling and ISR are regulated by HRI-responsive mitochondrial stress in Sertoli cells. Unexpectedly, we find that melatonin rescues the Cd-mediated disruption of BTB through blocking HRI-responsive mitochondrial stress in testes. Overall, these data indicate that environmental cadmium exposure impairs the BTB through activating HRI-responsive mitochondrial stress in Sertoli cells.

Gestational exposure to environmental cadmium induces placental apoptosis and fetal growth restriction via Parkin-modulated MCL-1 degradation.

Heavy metal cadmium (Cd), a classical environmental pollutant, causes placental apoptosis and fetal growth restriction (FGR), whereby the mechanism remains unclear. Here, our human case-control study firstly showed that there was a positive association of Parkin mitochondrial translocation, MCL-1 reduction, placental apoptosis, and all-cause FGR. Subsequently, Cd was administered to establish in vitro and in vivo models of placental apoptosis or FGR. Our models demonstrated that Parkin mitochondrial translocation was observed in Cd-administrated placental trophoblasts. Meaningfully, Parkin siRNA (siR) dramatically mitigated Cd-triggered apoptosis in placental trophoblasts. Mdivi-1 (M-1), an inhibitor for Parkin mitochondrial translocation, mitigated Cd-induced apoptosis in placental trophoblasts, which further ameliorated the effect of attenuated placental sizes in Cd-exposed mice. Furthermore, the interaction of MCL-1 with Parkin or Ub in Cd-stimulated cells was stronger than that in controls. MG132, an inhibitor for proteasome, abolished MCL-1 degradation in Cd-stimulated cells. Importantly, Parkin siR and M-1 memorably abolished the ubiquitin-dependent degradation of MCL-1 in placental trophoblasts. Interestingly, mito-TEMPO and melatonin, two mitochondria-targeted antioxidants, obviously rescued Cd-caused mitochondrial membrane potential (MMP) decrease, Parkin mitochondrial translocation, MCL-1 degradation, and apoptosis in placental trophoblasts. In conclusion, cadmium induces placental apoptosis and FGR via mtROS-mediated Parkin-modulated degradation of MCL-1.

Effects of external electric field on film growth, morphology, and nanostructure of polyelectrolyte and nanohybrid multilayers onto insulating substrates.

Electric-field-enhanced layer-by-layer (LbL) assembly of polyelectrolyte and nanohybrid multilayers onto insulating rigid substrates was successfully accomplished using two independent capacitor cells. The influence of external electric field on the multilayer formation was intensively investigated by UV-vis adsorption spectrometry, profilometry, atomic force microscopy, and small-angle X-ray diffraction. This approach was also attempted as a means of fabricating selective layer on flat sheet polymeric porous substrates to obtain the dense composite membranes with high pervaporation performance.

Balancing multiple objectives using a classification-based forest management system in Changbai Mountains, China.

Contemporary forest management often consists of multiple objectives, including restoration of human-impacted forested landscapes toward their range of natural variability (RNV) and sustainable levels of timber production. Balancing multiple management objectives is often challenging due to intrinsic conflicts between these objectives and a lack of reference conditions for evaluating the effectiveness of forest restoration efforts. We used a spatially explicit forest landscape model to assess how well a classification-based forest management (CFM) system could achieve multiple objectives in a Korean pine broadleaf mixed forest ecosystem at Changbai Mountain in Northeast China. The CFM system divided the forest landscape into three management areas (Commercial Forest, Special Ecological Welfare Forest, and General Ecological Welfare Forest), each with its own management objectives and prescriptions, but with an overall goal of increasing the ecological and economic sustainability of the entire landscape. The zoning approach adopted in the Chinese CFM system is very similar to the TRIAD approach that is being advocated for managing public forests in Canada. In this study, a natural disturbance scenario and seven harvest scenarios (one identical to the current harvest regime and six alternative scenarios) were simulated to examine how tree species composition, age structure, and timber production at the landscape level can be affected by different strategies under the CFM system. The results indicated that the current forest management regime would not only fail to reach the designated timber production level but also move the forest landscape far away from its RNV. In order to return the currently altered forest landscape to approach its RNV while providing a stable level of timber production over time, harvest intensities should be reduced to a level that is equivalent to the amount of timber removals that would occur under the natural disturbances; and the establishment of forest plantations is also required.

Major forest types and the evolution of sustainable forestry in China.

In this article, we introduce China's major forest types and discuss the historical development of forest management in China, including actions taken over the last decade toward achieving SMF. Major challenges are identified, and a strategy for SFM implementation in China is presented. China's forests consist of a wide variety of types with distinctive distributional patterns shaped by complex topography and multiple climate regimes. How to manage this wide array of forest resources has challenged forest managers and policy-makers since the founding of the country. Excessive exploitation of China's forest resources from the 1950s to the late 1990s contributed to environmental problems and calamities, such as floods, soil erosion, and desertification. At the start of the new millennium, the Chinese government decided to shift its emphasis from timber production towards the achievement of sustainable forest management (SFM). With a series of endeavors such as the implementation of the "Six Key Forestry Projects" and the reform of forest tenure policies, and the adoption of a classification system for China's forests, a beginning has been made at reversing the trend of environmental degradation that occurred throughout the latter half of the last century. At the same time, huge challenges remain to be tackled for the development of forestry in China.

Application of China's National Forest Continuous Inventory database.

The maintenance of a timely, reliable and accurate spatial database on current forest ecosystem conditions and changes is essential to characterize and assess forest resources and support sustainable forest management. Information for such a database can be obtained only through a continuous forest inventory. The National Forest Continuous Inventory (NFCI) is the first level of China's three-tiered inventory system. The NFCI is administered by the State Forestry Administration; data are acquired by five inventory institutions around the country. Several important components of the database include land type, forest classification and ageclass/ age-group. The NFCI database in China is constructed based on 5-year inventory periods, resulting in some of the data not being timely when reports are issued. To address this problem, a forest growth simulation model has been developed to update the database for years between the periodic inventories. In order to aid in forest plan design and management, a three-dimensional virtual reality system of forest landscapes for selected units in the database (compartment or sub-compartment) has also been developed based on Virtual Reality Modeling Language. In addition, a transparent internet publishing system for a spatial database based on open source WebGIS (UMN Map Server) has been designed and utilized to enhance public understanding and encourage free participation of interested parties in the development, implementation, and planning of sustainable forest management.

Forest management in Northeast China: history, problems, and challenges.

Studies of the history and current status of forest resources in Northeast China have become important in discussions of sustainable forest management in the region. Prior to 1998, excessive logging and neglected cultivation led to a series of problems that left exploitable forest reserves in the region almost exhausted. A substantial decrease in the area of natural forests was accompanied by severe disruption of stand structure and serious degradation of overall forest quality and function. In 1998, China shifted the primary focus of forest management in the country from wood production to ecological sustainability, adopting ecological restoration and protection as key foci of management. In the process, China launched the Natural Forest Conversion Program and implemented a new system of Classification-based Forest Management. Since then, timber harvesting levels in Northeast China have decreased, and forest area and stocking levels have slowly increased. At present, the large area of low quality secondary forest lands, along with high levels of timber production, present researchers and government agencies in China with major challenges in deciding on management models and strategies that will best protect, restore and manage so large an area of secondary forest lands. This paper synthesizes information from a number of sources on forest area, stand characteristics and stocking levels, and forest policy changes in Northeastern China. Following a brief historical overview of forest harvesting and ecological research in Northeast China, the paper discusses the current state of forest resources and related problems in forest management in the region, concluding with key challenges in need of attention in order to meet the demands for multi-purpose forest sustainability and management in the future.

Preparation and characterization of cross-linked starch nanocrystals and self-reinforced starch-based nanocomposite films.

In this study, starch-based nanocomposite films reinforced by cross-linked starch nanocrystals (CSNCs) were successfully prepared. CSNCs were obtained by cross-linking reaction between starch nanocrystals (SNCs) and sodium hexametaphosphate (SHMP). Through the characterization and comparison of SNCs and CSNCs in microscopic morphology, degree of substitution, swelling degree, XRD spectrum, and FTIR spectrum, the successful progress of the cross-linking reaction was confirmed. Besides, the effects of adding CSNCs on physiochemical properties of the nanocomposite films including mechanical properties, water vapor permeability, and contact angle were studied. The results confirmed that CSNCs had good enhancement effects on the physicochemical properties of starch-based films due to the self-reinforcing effect, and when the CSNCs content reached 10%, the nanocomposite film had the best overall performance. We further evaluated the cytotoxicity of the nanocomposite. Taken together, it is believed that the reported self-reinforced starch-based films are very promising for food packaging and preservation.