Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam.

Our aim was to develop a method for species diagnosis and to obtain data on the prevalence of Sarcocystis infections in cattle and water buffalo in the Son La Province of Northern Vietnam. Meat samples of naturally infected animals were examined by light and electron microscopy as well as by molecular methods. A PCR of part of the 18S rDNA gene followed by RFLP analysis was modified to detect infections with different Sarcocystis spp. in cattle and water buffaloes slaughtered in the Son La Province. It showed to be an economical method to detect multiple infections with Sarcocystis spp. Sequence analysis of the PCR amplicons was performed with selected samples and the results were compared with published sequences. With these methods the following Sarcocystis spp. were identified in cattle: Sarcocystis hirsuta, Sarcocystis cruzi and Sarcocystis hominis. Water buffaloes were infected with Sarcocystis fusiformis, S. cruzi, S. hominis and S. hirsuta. The results indicate that Sarcocystis spp. infecting cattle are also able to infect water buffaloes. So the validity of certain Sarcocystis spp. of water buffalo is discussed. Bovine lifestock in Northern Vietnam were commonly infected with Sarcocystis spp.

[Analysis of the trace contaminants in the atmosphere formed by the life-support environment of the closed system of man-higher plants-lower plants-microorganisms]. / Analiz mikroprimesei v atmosfere, formiruemoi sredoi obitaniia zamknutoi sistemy chelovek-vysshie rasteniia-nizshie rasteniia-mikroorganizmy.

Trace contaminants in the air of a biological life support system which was continuously complicated were examined. Air samples were taken by cooled traps and analyzed by gas chromatography. Changes in the air of the biological life support system as a function of its structure were compared using Hamming distance and composition similarity measures. Matrices of the measures were obtained and a graph whose structure corresponded to changes in the biological life support system was constructed. During prolonged experiments trace contaminants remained relatively stable and changed when a mineralization component was attached. It is concluded that the composition and variations in the organic components of the atmosphere can be used as an integral indicator of the function of the biological life support system as a whole.