RESUMO
Azaspiracid antibodies: Immunization of azaspiracid immunoconjugates has elicited monoclonal antibodies with distinct epitopes on the marine toxin; this will open the way toward azaspiracid diagnostics and the detection of contaminated shellfish before they can enter the food supply.
Assuntos
Anticorpos Monoclonais/química , Epitopos/química , Toxinas Marinhas/análise , Compostos de Espiro/análise , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/metabolismo , Ensaio de Imunoadsorção Enzimática , Epitopos/metabolismo , Imunoconjugados/imunologia , Imunoconjugados/metabolismo , Toxinas Marinhas/química , Toxinas Marinhas/imunologia , Camundongos , Frutos do Mar/toxicidade , Compostos de Espiro/química , Compostos de Espiro/imunologiaRESUMO
N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest k(cat), with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.
Assuntos
Anticorpos Antibacterianos/farmacologia , Bactérias Gram-Negativas/efeitos dos fármacos , Percepção de Quorum/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , 4-Butirolactona/análogos & derivados , 4-Butirolactona/fisiologia , Catálise , Hidrólise , Cinética , Pseudomonas aeruginosa/efeitos dos fármacos , Piocianina/biossínteseRESUMO
[reaction: see text] A new method of deprotection of N-p-methoxyphenylamines using anodic oxidation in acidic medium is presented. The process furnishes a high yield of amine and is compatible with several oxidable functional groups.