Exploratory decisions of Trinidadian guppies when uncertain about predation risk.

Animals can reduce their uncertainty of predation risk by gathering new information via exploration behaviour. However, a decision to explore may also be costly due to increased predator exposure. Here, we found contextual effects of predation risk on the exploratory activity of Trinidadian guppies Poecilia reticulata in a novel environment. First, guppies were exposed to a 3-day period of either high or low background predation risk in the form of repeated exposure to either injured conspecific cues (i.e. alarm cues) or control water, respectively. A day later, guppies were moved into a testing arena with limited visual information due to structural barriers and were then presented with an acute chemical stimulus, either alarm cues (a known and reliable indicator of risk), a novel odour (an ambiguous cue), or control water. In the presence of control water, guppies from high and low background risk showed a similar willingness to explore the arena. However, high-risk individuals significantly reduced their spatial evenness, although not their movement latency, in the presence of both the alarm and novel cues. When these high-risk individuals were a member of a shoal, they became willing to explore the environment more evenly in the presence of alarm cues while remaining cautious toward the novel cue, indicating an effect of the greater uncertainty associated with the novel cue. In contrast, low-risk guppies showed a willingness to explore the arena regardless of acute threat or social context. Such contextual effects of background risk and social context highlight the complexity of exploratory decisions when uncertain.

Retention of neophobic predator recognition in juvenile convict cichlids: effects of background risk and recent experience.

Exposure to conditions of elevated predation risk, even for relatively short periods, has been shown to induce neophobic responses to novel predators. Such phenotypically plastic responses should allow prey to exhibit costly anti-predator behaviour to novel cues only in situations where the risk of predation is high. While there is evidence that the level of background risk shapes the strength of induced neophobia, we know little about how long neophobic responses are retained. Here we exposed juvenile convict cichlids (Amatitlania nigrofasciata) to three background levels of short-term background risk and then tested their responses to novel predator odours. Cichlids exposed to low risk did not show neophobic responses, while those exposed to intermediate and high risk did. Using extinction trials, we demonstrate that the retention of neophobic responses is greater among cichlids exposed to high versus intermediate predation risk conditions. Moreover, we found much longer retention of the neophobic responses when cichlids were tested a single time compared to when they were tested repeatedly in the extinction trials. This work supports the prediction that neophobic responses to specific odours are relatively long lasting but can quickly wane if the cues are experienced repeatedly without them being associated with risk. It is clear that background level of risk and the frequency of exposure to novel cues are crucial factors in determining the retention of risk-related information among prey.

Assessing effects of predator density and diversity on neophobia in Trinidadian guppies.

Neophobic predator avoidance, where prey actively avoid novel stimuli, is thought to allow prey to cope with the inability to predict predation risk (i.e. uncertainty) while reducing the costs associated with learning. Recent studies suggest that neophobia is elicited as a response to unpredictable and elevated mean predation risk, and is linked to experience with diverse novel cues. However, no research has disentangled the effects of predator density and diversity on neophobia. We conditioned Trinidadian guppies (Poecilia reticulata) to high- or low-diversity predator model treatments paired with high, intermediate, or low concentrations of conspecific alarm cues as a proxy for predator density. We tested behavioural responses to a novel stimulus vs. a water control to determine differences in neophobia among treatments. We found that neophobic shoaling behaviour was shaped by mean risk (predator density). However both density and diversity shaped neophobic freezing, and to a weaker extent, neophobic area use. Our research suggests that predator diversity might elicit neophobic responses in guppies, but only when mean risk is high enough. The relationship between neophobia and components of predation risk is becoming increasingly relevant as ecological uncertainty becomes more prevalent with increasing climate change, anthropogenic impacts, and invasive species.