RESUMO
Chlamydia trachomatis infection is the most common sexually transmitted disease among women. The aim of this study was to determine by PCR the incidence of C. trachomatis among young women in Northern Sardinia since no studies are present in this area. The results obtained showed a moderate increase of chlamydial infection since 1997.
Assuntos
Infecções por Chlamydia/epidemiologia , Chlamydia trachomatis/isolamento & purificação , Adulto , Colo do Útero/microbiologia , Infecções por Chlamydia/microbiologia , Chlamydia trachomatis/genética , Feminino , Humanos , Incidência , Itália/epidemiologia , Reação em Cadeia da Polimerase/métodos , Manejo de Espécimes/métodos , Vagina/microbiologiaRESUMO
The aim of this study was to produce Lantana camara essential oil by SFE and to study the effect of matrix grinding on the yield and/or composition of the extract. Experiments were carried out on grinding matrices (G) and on not grinding matrices (NG). The extracts were analyzed by GC-MS. A comparison with the oil obtained by hydrodistillation is also given. Finally, the antibacterial and antifungal activity of the various extracts has been assayed.
Assuntos
Antibacterianos/isolamento & purificação , Antifúngicos/isolamento & purificação , Lantana/química , Óleos Voláteis/isolamento & purificação , Óleos de Plantas/isolamento & purificação , Antibacterianos/química , Antibacterianos/farmacologia , Antifúngicos/química , Antifúngicos/farmacologia , Cromatografia com Fluido Supercrítico , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Folhas de Planta/química , Óleos de Plantas/química , Óleos de Plantas/farmacologiaRESUMO
We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.
Assuntos
Mycobacterium tuberculosis/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Tuberculose/microbiologia , Elementos de DNA Transponíveis/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Humanos , Mycobacterium tuberculosis/genética , Sensibilidade e Especificidade , Taq Polimerase/químicaAssuntos
Antibacterianos , Antifúngicos , Candida/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Inula/química , Óleos Voláteis/farmacologia , Cromatografia com Fluido Supercrítico , Testes de Sensibilidade Microbiana , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Raízes de Plantas/químicaRESUMO
BACKGROUND: Recently, the dissemination of ESBL (PER-1) among Acinetobacter isolates was reported in Turkish hospitals. We investigated the presence and the association of various virulence determinants in 20 Acinetobacter baumannii isolates, of which 13 were blaPER-1 positive. MATERIAL/METHODS: Virulence tests were slime and hemolysin production, gelatinase and protease activity, biofilm formation, and Caco2 cell adhesion. RAPD analysis was also performed with ERIC primers. RESULTS: None of the strains was positive for slime or hemolysin production and gelatinase or protease activity. A total of 16 strains, five of which were PER-1 gene negative, formed a biofilm on polymer surfaces. There was no relation between the presence of the PER gene and biofilm formation. On the other hand, nine strains (all PER-1 gene positive) were positive in adhesion experiments to Caco2 cell lines. All PER-negative strains were negative for cell adhesion. CONCLUSIONS: This study indicates the existence of a relation between PER-1 gene and cell adhesion in Acinetobacter strains.