RESUMO
Elevations of sex steroids induced by social cues can rapidly modulate social behavior, but we know little about where they act within the nervous system to produce such effects. In male goldfish, testosterone (T) rapidly increases approach responses to the visual cues of females through its conversion to estradiol. Because aromatase is expressed in the retina, we tested if T can acutely influence retina responses to visual stimuli, and investigated the receptor mechanisms that may mediate such effects. Specifically, we measured FOS protein immunoreactivity to determine if T affects cellular responses to visual stimuli that include females, and used electrophysiology to investigate whether T can generally affect light sensitivity. We found that T acutely increased FOS responses to the simultaneous onset of light and the presence of female visual stimuli, both of which would normally be associated with early morning spawning, and increased electrophysiological responses to low intensity light pulses. Both effects were blocked by an estrogen receptor beta (ERß) antagonist, indicating that T is likely being converted to estradiol (E2) and acting through an ERß mediated mechanism to acutely modulate visual processing. Changes in sensory processing could subsequently influence approach behavior to increase reproductive success in competitive mating environments.
Assuntos
Receptor beta de Estrogênio/metabolismo , Proteínas de Peixes/metabolismo , Carpa Dourada/metabolismo , Retina/metabolismo , Testosterona/metabolismo , Percepção Visual/fisiologia , Animais , Comportamento Competitivo/fisiologia , Antagonistas do Receptor de Estrogênio/farmacologia , Receptor beta de Estrogênio/antagonistas & inibidores , Feminino , Proteínas de Peixes/antagonistas & inibidores , Masculino , Proteínas Proto-Oncogênicas c-fos/metabolismo , Reprodução/fisiologia , Retina/efeitos dos fármacos , Comportamento Sexual Animal/fisiologia , Percepção Visual/efeitos dos fármacosRESUMO
Mechanical and neurophysiological anisotropies mediate three-dimensional responses of the heart of ITALIC! Homarus americanus Although hearts ITALIC! in vivoare loaded multi-axially by pressure, studies of invertebrate cardiac function typically use uniaxial tests. To generate whole-heart length-tension curves, stretch pyramids at constant lengthening and shortening rates were imposed uniaxially and biaxially along longitudinal and transverse axes of the beating whole heart. To determine whether neuropeptides that are known to modulate cardiac activity in ITALIC! H. americanusaffect the active or passive components of these length-tension curves, we also performed these tests in the presence of SGRNFLRFamide (SGRN) and GYSNRNYLRFamide (GYS). In uniaxial and biaxial tests, both passive and active forces increased with stretch along both measurement axes. The increase in passive forces was anisotropic, with greater increases along the longitudinal axis. Passive forces showed hysteresis and active forces were higher during lengthening than shortening phases of the stretch pyramid. Active forces at a given length were increased by both neuropeptides. To exert these effects, neuropeptides might have acted indirectly on the muscle via their effects on the cardiac ganglion, directly on the neuromuscular junction, or directly on the muscles. Because increases in response to stretch were also seen in stimulated motor nerve-muscle preparations, at least some of the effects of the peptides are likely peripheral. Taken together, these findings suggest that flexibility in rhythmic cardiac contractions results from the amplified effects of neuropeptides interacting with the length-tension characteristics of the heart.
Assuntos
Anisotropia , Nephropidae/fisiologia , Neurotransmissores/farmacologia , Estresse Mecânico , Sequência de Aminoácidos , Animais , Fenômenos Biomecânicos/efeitos dos fármacos , Gânglios dos Invertebrados/efeitos dos fármacos , Gânglios dos Invertebrados/fisiologia , Coração/efeitos dos fármacos , Nephropidae/efeitos dos fármacos , Neuropeptídeos/química , Neuropeptídeos/farmacologia , Perfusão , Cloreto de SódioRESUMO
In insects, a family of peptides with sequence homology to the vertebrate calcitonins has been implicated in the control of diuresis, a process that includes mixing of the hemolymph. Here, we show that a member of the insect calcitonin-like diuretic hormone (CLDH) family is present in the American lobster, Homarus americanus, serving, at least in part, as a powerful modulator of cardiac output. Specifically, during an ongoing EST project, a transcript encoding a putative H. americanus CLDH precursor was identified; a full-length cDNA was subsequently cloned. In silico analyses of the deduced prepro-hormone predicted the mature structure of the encoded CLDH to be GLDLGLGRGFSGSQAAKHLMGLAAANFAGGPamide (Homam-CLDH), which is identical to a known Tribolium castaneum peptide. RT-PCR tissue profiling suggests that Homam-CLDH is broadly distributed within the lobster nervous system, including the cardiac ganglion (CG), which controls the movement of the neurogenic heart. RT-PCR analysis conducted on pacemaker neuron- and motor neuron-specific cDNAs suggests that the motor neurons are the source of the CLDH message in the CG. Perfusion of Homam-CLDH through the isolated lobster heart produced dose-dependent increases in both contraction frequency and amplitude and a dose-dependent decrease in contraction duration, with threshold concentrations for all parameters in the range 10(-11) to 10(-10) mol l(-1) or less, among the lowest for any peptide on this system. This report is the first documentation of a decapod CLDH, the first demonstration of CLDH bioactivity outside the Insecta, and the first detection of an intrinsic neuropeptide transcript in the crustacean CG.
Assuntos
Calcitonina/análogos & derivados , Hormônios/isolamento & purificação , Hormônios/metabolismo , Nephropidae/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Débito Cardíaco , Clonagem Molecular , DNA Complementar/genética , Diuréticos/análise , Diuréticos/isolamento & purificação , Diuréticos/metabolismo , Hormônios/análise , Hormônios/genética , Dados de Sequência Molecular , Miocárdio/químicaRESUMO
pQDLDHVFLRFamide is a highly conserved crustacean neuropeptide with a structure that places it within the myosuppressin subfamily of the FMRFamide-like peptides. Despite its apparent ubiquitous conservation in decapod crustaceans, the paracrine and/or endocrine roles played by pQDLDHVFLRFamide remain largely unknown. We have examined the actions of this peptide on the cardiac neuromuscular system of the American lobster Homarus americanus using four preparations: the intact animal, the heart in vitro, the isolated cardiac ganglion (CG), and a stimulated heart muscle preparation. In the intact animal, injection of myosuppressin caused a decrease in heartbeat frequency. Perfusion of the in vitro heart with pQDLDHVFLRFamide elicited a decrease in the frequency and an increase in the amplitude of heart contractions. In the isolated CG, myosuppressin induced a hyperpolarization of the resting membrane potential of cardiac motor neurons and a decrease in the cycle frequency of their bursting. In the stimulated heart muscle preparation, pQDLDHVFLRFamide increased the amplitude of the induced contractions, suggesting that myosuppressin modulates not only the CG, but also peripheral sites. For at least the in vitro heart and the isolated CG, the effects of myosuppressin were dose-dependent (10(-9) to 10(-6) mol l(-1) tested), with threshold concentrations (10(-8)-10(-7) mol l(-1)) consistent with the peptide serving as a circulating hormone. Although cycle frequency, a parameter directly determined by the CG, consistently decreased when pQDLDHVFLRFamide was applied to all preparation types, the magnitudes of this decrease differed, suggesting the possibility that, because myosuppressin modulates the CG and the periphery, it also alters peripheral feedback to the CG.
Assuntos
Crustáceos/química , Coração/efeitos dos fármacos , Nephropidae/efeitos dos fármacos , Nephropidae/fisiologia , Sistema Nervoso/efeitos dos fármacos , Neuropeptídeos/farmacologia , Hormônios Peptídicos/farmacologia , Sequência de Aminoácidos , Animais , Sequência de Bases , FMRFamida/farmacologia , Gânglios dos Invertebrados/efeitos dos fármacos , Gânglios dos Invertebrados/fisiologia , Frequência Cardíaca/efeitos dos fármacos , Dados de Sequência Molecular , Contração Miocárdica/efeitos dos fármacos , Miocárdio , Neuropeptídeos/química , Neuropeptídeos/genética , Hormônios Peptídicos/química , Perfusão , Reprodutibilidade dos Testes , Fatores de TempoRESUMO
We tested whether the sex pheromones that stimulate courtship clasping in male roughskin newts do so, at least in part, by amplifying the somatosensory signals that directly trigger the motor pattern associated with clasping and, if so, whether that amplification is dependent on endogenous vasotocin (VT). Female olfactory stimuli increased the number of action potentials recorded in the medulla of males in response to tactile stimulation of the cloaca, which triggers the clasp motor reflex, as well as to tactile stimulation of the snout and hindlimb. That enhancement was blocked by exposing the medulla to a V1a receptor antagonist before pheromone exposure. However, the antagonist did not affect medullary responses to tactile stimuli in the absence of pheromone exposure, suggesting that pheromones amplify somatosensory signals by inducing endogenous VT release. The ability of VT to couple sensory systems together in response to social stimulation could allow this peptide to induce variable behavioural outcomes, depending on the immediate context of the social interaction and thus on the nature of the associated stimuli that are amplified. If widespread in vertebrates, this mechanism could account for some of the behavioural variability associated with this and related peptides both within and across species.
Assuntos
Bulbo/metabolismo , Salamandridae/fisiologia , Atrativos Sexuais/farmacologia , Vasotocina/fisiologia , Potenciais de Ação/efeitos dos fármacos , Animais , Antagonistas dos Receptores de Hormônios Antidiuréticos , Cloaca/fisiologia , Eletrofisiologia , Feminino , Masculino , Bulbo/citologia , Bulbo/fisiologia , Neurônios Aferentes/efeitos dos fármacos , Neurônios Aferentes/fisiologia , Estimulação Física , Receptores de Vasopressinas , Atrativos Sexuais/fisiologia , Comportamento Sexual Animal , Estimulação QuímicaRESUMO
In recent years, as our understanding of the pattern-generating networks responsible for a variety of behaviors has increased, the interactions of multiple neural networks have been examined in a number of systems. These studies have shown that functionally related pattern generators can interact extensively, and that the extent to which two or more of these networks interact is not fixed, but can be altered by neuromodulators. Furthermore, a number of studies have begun to elucidate the mechanisms responsible for those interactions. In the crustacean stomatogastric system, for example, neurons can switch between different pattern generators, and whole networks can fuse into single patterns. In addition, several networks can be dismantled, and their components used to generate a new network. The mechanisms responsible for these changes are the same as those involved in other circuit re-configurations, namely alterations of both intrinsic membrane properties of component neurons and alterations in the strength of synapses within the networks.
Assuntos
Comportamento Animal/fisiologia , Comportamento/fisiologia , Rede Nervosa/fisiologia , Periodicidade , Animais , Humanos , Rede Nervosa/citologia , Neurônios/fisiologiaRESUMO
Organotypic cultures of the lobster (Homarus gammarus) stomatogastric nervous system (STNS) were used to assess changes in membrane properties of neurons of the pyloric motor pattern-generating network in the long-term absence of neuromodulatory inputs to the stomatogastric ganglion (STG). Specifically, we investigated decentralization-induced changes in the distribution and density of the transient outward current, I(A), which is encoded within the STG by the shal gene and plays an important role in shaping rhythmic bursting of pyloric neurons. Using an antibody against lobster shal K(+) channels, we found shal immunoreactivity in the membranes of neuritic processes, but not somata, of STG neurons in 5 d cultured STNS with intact modulatory inputs. However, in 5 d decentralized STG, shal immunoreactivity was still seen in primary neurites but was likewise present in a subset of STG somata. Among the neurons displaying this altered shal localization was the pyloric dilator (PD) neuron, which remained rhythmically active in 5 d decentralized STG. Two-electrode voltage clamp was used to compare I(A) in synaptically isolated PD neurons in long-term decentralized STG and nondecentralized controls. Although the voltage dependence and kinetics of I(A) changed little with decentralization, the maximal conductance of I(A) in PD neurons increased by 43.4%. This increase was consistent with the decentralization-induced increase in shal protein expression, indicating an alteration in the density and distribution of functional A-channels. Our results suggest that, in addition to the short-term regulation of network function, modulatory inputs may also play a role, either directly or indirectly, in controlling channel number and distribution, thereby maintaining the biophysical character of neuronal targets on a long-term basis.
Assuntos
Relógios Biológicos/fisiologia , Canais Iônicos/metabolismo , Neurônios Motores/metabolismo , Neurotransmissores/metabolismo , Canais de Potássio de Abertura Dependente da Tensão da Membrana , Animais , Anticorpos/farmacologia , Relógios Biológicos/efeitos dos fármacos , Membrana Celular/metabolismo , Sistema Digestório/inervação , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/efeitos dos fármacos , Gânglios dos Invertebrados/metabolismo , Técnicas In Vitro , Canais Iônicos/antagonistas & inibidores , Neurônios Motores/efeitos dos fármacos , Nephropidae , Rede Nervosa/citologia , Rede Nervosa/efeitos dos fármacos , Rede Nervosa/metabolismo , Neuritos/metabolismo , Técnicas de Patch-Clamp , Periodicidade , Potássio/metabolismo , Bloqueadores dos Canais de Potássio , Canais de Potássio/metabolismo , Canais de Potássio ShalRESUMO
The firing of a single neuron (named anterior pyloric modulator: APM) of the esophageal ganglion considerably modifies the pyloric rhythm of the rock lobster. These modifications, characterized by a long delay to onset and a long duration, include increased frequency and amplitude of oscillations of the motor neurons, changes in the efficacy of certain synapses within the network, and voltage-dependent modifications of membrane properties of some motor neurons. APM thus seems to be a true modulatory neuron. The APM-provoked changes resemble changes seen in the whole animal, making this a suitable system for an analysis of modulation on several levels.
Assuntos
Crustáceos/fisiologia , Neurônios/fisiologia , Animais , Esôfago/inervação , Gânglios/fisiologia , Nephropidae/fisiologia , Piloro/inervação , Piloro/fisiologiaRESUMO
1. The cardiac sac motor pattern consists of slow and irregular impulse bursts in the motor neurons [cardiac sac dilator 1 and 2 (CD1 and CD2)] that innervate the dilator muscles of the cardiac sac region of the crustacean foregut. 2. The effects of the peptides, proctolin and red pigment-concentrating hormone (RPCH), on the cardiac sac motor patterns produced by in vitro preparations of the combined stomatogastric nervous system [the stomatogastric ganglion (STG), the paired commissural ganglia (CGs), and the oesophageal ganglion (OG)] were studied. 3. Bath applications of either RPCH or proctolin activated the cardiac sac motor pattern when this motor pattern was not already active and increased the frequency of the cardiac sac motor pattern in slowly active preparations. 4. The somata of CD1 and CD2 are located in the esophageal and stomatogastric ganglia, respectively. Both neurons project to all four of the ganglia of the stomatogastric nervous system. RPCH elicited cardiac sac motor patterns when applied to any region of the stomatogastric nervous system, suggesting a distributed pattern generating network with multiple sites of modulation. 5. The anterior median (AM) neuron innervates the constrictor muscles of the cardiac sac. The AM usually functions as a part of the gastric mill pattern generator. However, when the cardiac sac is activated by RPCH applied to the stomatogastric ganglion, the AM neuron becomes active in antiphase with the cardiac sac dilator bursts. This converts the cardiac sac motor pattern from a one-phase rhythm to a two-phase rhythm. 6. These data show that a neuropeptide can cause a neuronal element to switch from being solely a component of one neuronal circuit to functioning in a second one as well. This example shows that peptidergic "reconfiguration" of neuronal networks can produce substantial changes in the behavior of associated neurons.
Assuntos
Coração/fisiologia , Nephropidae/fisiologia , Neuropeptídeos , Oligopeptídeos/farmacologia , Peptídeos/fisiologia , Potenciais de Ação , Animais , Feminino , Coração/efeitos dos fármacos , Imuno-Histoquímica , Masculino , Neurônios Motores/efeitos dos fármacos , Neurônios Motores/fisiologia , Vias Neurais , Ácido Pirrolidonocarboxílico/análogos & derivadosRESUMO
In the lobsters Fasus lalandii and Palinurus vulgaris, the rhythmical activity of the pyloric pattern generator of the stomatogastric nervous system is strongly modified by the firing of a single identified interneurone, whose activity we have recorded from the cell body, in vitro. The cell body of this interneurone, the anterior pyloric modulator (APM), is located in the oesophageal ganglion and sends two axons to the stomatogastric ganglion via the inferior oesophageal nerves, the commissural ganglia, the superior oesophageal nerves and the stomatogastric nerve. Firing of neurone APM modifies the activity of all the neurones of the pyloric network, including pacemaker and follower neurones. Its effects are both quantitative (increase in the frequency of the rhythm and in the frequency of spikes within cell bursts) and qualitative (modifications in relative efficacies of the synaptic relationships within the pyloric network, which in turn lead to changes in the phase relationships between the discharges of the neurones). The effects on pyloric activity induced by firing of neurone APM are established slowly (one or two seconds) and are of long duration (ten times the duration of APM's discharge). These modifications most probably involve muscarinic cholinergic receptors. APM's influences on the activity of pyloric neurones appear to be characteristic of a neuromodulatory process and are such that they may be of behavioural significance in the intact animal.
Assuntos
Sistema Digestório/inervação , Interneurônios/fisiologia , Animais , Axônios/fisiologia , Condutividade Elétrica , Feminino , Gânglios/fisiologia , Masculino , Nephropidae , Sinapses/fisiologiaRESUMO
In the isolated stomatogastric nervous system of the lobster Fasus lalandii, the strong modifications of the pyloric motor pattern induced by firing of the single anterior pyloric modulator neurone (APM) are due primarily to modulation by APM activity of the regenerative membrane properties which are responsible for the 'burstiness' of all the pyloric neurones and particularly of the non-pacemaker neurones (constrictor motoneurones). This modulation has been studied under experimental conditions where the main extrinsic influences usually received by the pyloric constrictor neurones (intra-network synaptic interactions, activity of pacemaker neurones, and phasic central inputs from two premotor centres) are minimal. Under these conditions a brief discharge of neurone APM induces long plateaus of firing in all of the pyloric neurones. The non-pacemaker neurones of the pyloric network are not simply passive follower neurones, but can produce regenerative depolarizations (plateau potentials) during which the neurones fire spikes. The ability of the pyloric constrictor neurones to produce plateau potentials (plateau properties) contributes greatly to the generation of the rhythmical pyloric motor pattern. When these neurones spontaneously express their plateau properties, firing of neurone APM amplifies these properties. When most of the central inputs usually received by the pyloric constrictor neurones are experimentally suppressed, these neurones can no longer produce plateau potentials. In such conditions, firing of the single modulatory neurone APM can reinduce plateau properties of the pyloric constrictor neurones. In addition, firing in APM neurone slows down the active repolarization phase which terminates the plateau potentials of pyloric constrictor neurones. This effect is long-lasting and voltage-dependent. Modulation by APM of the plateau properties of the pyloric neurones also changes the sensitivity of these neurones to synaptic inputs. This effect can explain the strong modifications that an APM discharge exerts on a current pyloric motor pattern. Moreover, it might render the motoneurones of the pyloric pattern generator more sensitive to inputs from a command oscillator, and contribute to switching on the pyloric motor pattern.
Assuntos
Sistema Digestório/inervação , Interneurônios/fisiologia , Animais , Condutividade Elétrica , Esôfago/inervação , Feminino , Masculino , Potenciais da Membrana , NephropidaeRESUMO
1. One response of the terrestrial slug, Limax maximus to dehydration is the initiation and modulation of the pneumostome rhythm. When a slug has lost 15-20% of its initial body weight by evaporation, the frequency of pheumostome closures, which is less than 0.5 closures/min in fully hydrated slugs, begins to increase. 2. The frequency increases with further dehydration, but the average duration of each closure remains constant. Thus, the proportion of time during which the pneumostome is closed increases. Simultaneously, the area of the pneumostome opening decreases. 3. This behavior appears to be controlled in part by both the osmolality of the slug's hemolymph and by a peptide closely related to arginine vasotocin (AVT) and arginine vasopressin (AVP). Injecting intact slugs with mannitol, which increases the osmolality of the hemolymph, or with AVT or AVP, can initiate the pneumostome rhythm. 4. Mannitol injections, however, do not provoke the decrease in the area of the pneumostome opening which is induced by natural dehydration or by AVT or AVP injection. This suggests that at least two systems may be involved in the overall control of the pneumostome.
Assuntos
Arginina Vasopressina/fisiologia , Moluscos/fisiologia , Vasotocina/fisiologia , Animais , Desidratação , Hemolinfa/fisiologia , Manitol/farmacologia , Concentração Osmolar , Equilíbrio HidroeletrolíticoRESUMO
Recordings from the lateral gastric (LG) neuron, which forms part of the gastric mill central pattern generator in the red lobster, Palinurus vulgaris, indicate that regenerative membrane properties (plateau properties) and synaptic inputs interact sequentially rather than simultaneously to determine its discharge pattern. LG thus presents a composite discharge, consisting of 2 separate segments of firing and one silent period. The first firing segment depends on regenerative membrane properties; this is the endogenous component, or segment, of LG's discharge. The second firing segment is the result of extrinsic synaptic input, forming the synaptic component of LG's discharge. The relative importance of these 2 components can vary, and thus LG's discharge ranges from one in which LG fires only as a result of its endogenous component to one in which its endogenous component is entirely absent and only the synaptic component underlies action potentials. Activity in an identified modulatory neuron suppresses the endogenous segment and enhances the synaptic segment of LG's discharge. This long-lasting effect in turn changes phase relationships within the gastric mill network and provides mechanisms for producing flexibility in the gastric pattern generator and for ensuring that a specific motor output is generated by a flexible neural network.
Assuntos
Fenômenos Fisiológicos do Sistema Nervoso , Neurônios/fisiologia , Sinapses/fisiologia , Potenciais de Ação , Animais , Eletrofisiologia , Feminino , Interneurônios/fisiologia , Masculino , Nephropidae , Regeneração Nervosa , Estômago/inervaçãoRESUMO
Animals make many different movements as circumstances dictate. These movements often involve the coordination of several neural networks, the output of which can be changed by modulatory substances. Here we report that the neuropeptide red pigment concentrating hormone modulates the interactions between two rhythmic pattern-generating networks in the lobster stomatogastric nervous system. Red pigment concentrating hormone markedly enhances the amplitude of synaptic interactions between elements of two pattern-generating networks--the cardiac sac and the gastric mill. Consequently, two networks operating under some circumstances virtually independently can be fused into one functional unit operating differently from either of the two original networks. These results show how a neuropeptide can alter the functional configuration of a neural network so that widely disparate outputs can be produced by the same neurons.
Assuntos
Gânglios/fisiologia , Hormônios de Invertebrado/fisiologia , Neurônios Motores/fisiologia , Neuropeptídeos/fisiologia , Oligopeptídeos/fisiologia , Animais , Potenciais da Membrana , Modelos Neurológicos , Nephropidae , Fenômenos Fisiológicos do Sistema Nervoso , Ácido Pirrolidonocarboxílico/análogos & derivadosRESUMO
The neuropeptide red pigment concentrating hormone (RPCH), which we have previously shown to activate the cardiac sac motor pattern and lead to a conjoint gastric mill-cardiac sac pattern in the spiny lobster Panulirus, also activates and modulates the pyloric pattern. Like the activity of gastric mill neurons in RPCH, the pattern of activity in the pyloric neurons is considerably more complex than that seen in control saline. This reflects the influence of the cardiac sac motor pattern, and particularly the upstream inferior ventricular (IV) neurons, on many of the pyloric neurons. RPCH intensifies this interaction by increasing the strength of the synaptic connections between the IV neurons and their targets in the stomatogastric ganglion. At the same time, RPCH enhances postinhibitory rebound in the lateral pyloric (LP) neuron. Taken together, these factors largely explain the complex pyloric pattern recorded in RPCH in Panulirus.
Assuntos
Hormônios de Invertebrado/farmacologia , Nephropidae/fisiologia , Rede Nervosa/efeitos dos fármacos , Rede Nervosa/fisiologia , Oligopeptídeos/farmacologia , Piloro/inervação , Animais , Eletrofisiologia , Feminino , Coração/fisiologia , Masculino , Inibição Neural/fisiologia , Neurônios/fisiologia , Oscilometria , Ácido Pirrolidonocarboxílico/análogos & derivados , Valores de Referência , Transmissão Sináptica/efeitos dos fármacosRESUMO
1. The cardiac sac network, which controls the rhythmic contractions of the cardiac sac in the foregut of crustaceans, is distributed throughout the stomatogastric nervous system, including the oesophageal ganglion (OG), the commissural ganglia (CGs), and the stomatogastric ganglion (STG). A red pigment-concentrating hormone (RPCH)-like peptide is likewise widely distributed. 2. The effects that bath application of the neuropeptide RPCH to the different ganglia has on the cardiac sac pattern were studied. 3. RPCH applied to the STG, the OG, or the CGs elicited bursting activity in all the known components of the cardiac sac pattern, including the two motor neurons, cardiac sac dilators 1 and 2 (CD1 and CD2), and the inferior ventricular nerve (ivn) fibers. 4. A cardiac sac pattern was also elicited when RPCH was applied to either the STG, the OG, or the CGs after synapses in that ganglion had been blocked by low Ca2+ saline containing 20 mM Co2+. 5. These data suggest that the ivn fibers are sensitive to RPCH and respond to it by generating bursting activity at or near their terminals in all four ganglia. 6. Application of RPCH to either the STG or the OG also caused an increase in the amplitude of the postsynaptic potential (PSP) from the ivn fibers to both CD1 and CD2. The increase was largest in the ganglion to which the RPCH was applied. 7. Repeated stimulation of the ivn, mimicking the bursts that occur during cardiac sac activity, also caused an increase in PSP amplitude, and so facilitation resulting from activation of ivn bursting could account for a portion of the increased amplitude seen in RPCH.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Sistema Nervoso Central/fisiologia , Gânglios/fisiologia , Motilidade Gastrointestinal/fisiologia , Hormônios de Invertebrado/fisiologia , Nephropidae/fisiologia , Rede Nervosa/fisiologia , Oligopeptídeos/fisiologia , Animais , Técnicas de Cultura , Feminino , Masculino , Potenciais da Membrana/fisiologia , Fibras Nervosas/fisiologia , Neurônios/fisiologia , Ácido Pirrolidonocarboxílico/análogos & derivadosRESUMO
Two of the peptides found in the stomatogastric nervous system of the spiny lobster, Panulirus interruptus, interacted to modulate the activity of the cardiac sac motor pattern. In the isolated stomatogastric ganglion, red-pigment-concentrating hormone (RPCH), but not proctolin, activated the bursting activity in the inferior ventricular (IV) neurons that drives the cardiac sac pattern. The cardiac sac pattern normally ceased within 15 min after the end of RPCH superfusion. However, when proctolin was applied within a few minutes of that time, it was likewise able to induce cardiac sac activity. Similarly, proctolin applied together with subthreshold RPCH induced cardiac sac bursting. The amplitude of the excitatory postsynaptic potentials from the IV neurons to the cardiac sac dilator neuron CD2 (1 of the 2 major motor neurons in the cardiac sac system) was potentiated in the presence of both proctolin and RPCH. The potentiation in RPCH was much greater than in proctolin alone. However, the potentiation in proctolin after RPCH was equivalent to that recorded in RPCH alone. Although we do not yet understand the mechanisms for these interactions of the two modulators, this study provides an example of one factor that can determine the "state" of the system that is critical in determining the effect of a modulator that is "state dependent," and it provides evidence for yet another level of flexibility in the motor output of this system.
Assuntos
Sistema Digestório/efeitos dos fármacos , Neuropeptídeos , Neurotransmissores/farmacologia , Oligopeptídeos/farmacologia , Animais , Fenômenos Fisiológicos do Sistema Digestório , Relação Dose-Resposta a Droga , Feminino , Masculino , Nephropidae , Técnicas de Patch-Clamp , Ácido Pirrolidonocarboxílico/análogos & derivadosRESUMO
The requirement for trophic factors in neurite outgrowth is well established, though their role in synapse formation is yet to be determined. Moreover, the issue of whether the trophic factors mediating neurite outgrowth are also responsible for synapse specification has not yet been resolved. To test whether trophic factors mediating neurite outgrowth and synapse formation between identified neurons are conserved in two molluscan species and whether these developmental processes are differentially regulated by different trophic factors, we used soma-soma and neurite-neurite synapses between identified Lymnaea neurons. We demonstrate here that the trophic factors present in Aplysia hemolymph, although sufficient to induce neurite outgrowth from Lymnaea neurons, do not promote specific synapse formation between excitatory partners. Specifically, the identified presynaptic neuron visceral dorsal 4 (VD4) and postsynaptic neuron left pedal dorsal 1 (LPeD1) were either paired in a soma-soma configuration or plated individually to allow neuritic contacts. Cells were cultured in either Lymnaea brain-conditioned medium (CM) or on poly-L-lysine dishes that were pretreated with Aplysia hemolymph (ApHM), but contained only Lymnaea defined medium (DM; does not promote neurite outgrowth). In ApHM-coated dishes containing DM, Lymnaea neurons exhibited extensive neurite outgrowth, but appropriate excitatory synapses failed to develop between the cells. Instead, inappropriate reciprocal inhibitory synapses formed between VD4 and LPeD1. Similar inappropriate inhibitory synapses were observed in Aplysia hemolymph-pretreated dishes that contained dialyzed Aplysia hemolymph. These inhibitory synapses were novel and inappropriate, because they do not exist in vivo. A receptor tyrosine kinase inhibitor (Lavendustin A) blocked neurite outgrowth induced by both Lymnaea CM and ApHM. However, it did not affect inappropriate inhibitory synapse formation between the neurons. These data demonstrate that neurite outgrowth but not inappropriate inhibitory synapse formation involves receptor tyrosine kinases. Together, our data provide direct evidence that trophic factors required for neurite outgrowth are conserved among two different molluscan species, and that neurite extension and synapse specification between excitatory partners are likely mediated by different trophic factors.