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Objective: To screen and analyze the key differentially expressed genes characteristics in nonalcoholic fatty liver disease (NAFLD) with bioinformatics method. Methods: NAFLD-related expression matrix GSE89632 was downloaded from the GEO database. Limma package was used to screen differentially expressed genes (DEGs) in healthy, steatosis (SS), and nonalcoholic steatohepatitis (NASH) samples. WGCNA was used to analyze the output gene module. The intersection of module genes and differential genes was used to determine the differential genes characteristic, and then GO function and KEGG signaling pathway enrichment analysis were performed. The protein-protein interaction network (PPI) was constructed using the online website STRING and Cytoscape software, and the key (Hub) genes were screened. Finally, R software was used to analyze the receiver operating characteristic curve (ROC) of the Hub gene. Results: 92 differentially expressed genes characteristic were obtained through screening, which were mainly enriched in inflammatory response-related functions of "lipopolysaccharide response and molecular response of bacterial origin", as well as cancer signaling pathways of "proteoglycan in cancer" and "T-cell leukemia virus infection-related". 10 hub genes (FOS, CXCL8, SERPINE1, CYR61, THBS1, FOSL1, CCL2, MYC, SOCS3 and ATF3) had good diagnostic value. Conclusion: The differentially expressed hub genes among the 10 NAFLD disease-related characteristics obtained with bioinformatics analysis may become a diagnostic and prognostic marker and potential therapeutic target for NAFLD. However, further basic and clinical studies are needed to validate.
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Hepatopatia Gordurosa não Alcoólica , Biologia Computacional/métodos , Perfilação da Expressão Gênica/métodos , Redes Reguladoras de Genes , Humanos , Hepatopatia Gordurosa não Alcoólica/genética , Mapas de Interação de Proteínas/genéticaRESUMO
Objective: To evaluate the oncologic outcomes of different laparoscopic radical hysterectomy. Methods: From January 2011 to December 2014, the laparoscopic operation cases of cervical cancer at stage â b1, â b2, â ¡a1 and â ¡a2, including the histologic subtypes of squamous-cell carcinoma, adenocarcinoma and adenosquamous carcinoma, were collected in five clinical centers. The data were divided into two groups according to the surgical procedures, that is, modified laparoscopic-vaginal radical hysterectomy (mLVRH) and total laparoscopic radical hysterectomy (TLRH). The overall survival rate (OS), disease-free survival rate (DFS) at 5 years were retrospectively analyzed in this study. Results: There were 674 cases in total, including 377 cases of mLVRH, 297 cases of TLRH. (1) The OS at 5 years: the mLVRH was 96.1% and the TLRH was 92.0%, and the mLVRH was higher than that of TLRH ï¼P=0.010ï¼. Stratify analysisï¼ including stage of disease ï¼â b1 and â ¡a1ï¼ï¼ histologic subtypes ï¼squamous-cell carcinoma, adenocarcinomaï¼ï¼ lymph node metastasisï¼ revealed that, â Stage of disease: in stage â b1, the OS at five years of mLVRH was higher than that in TLRH group ï¼98.6% vs 93.6%, P=0.012ï¼. In stage â ¡a1, there was significant difference between the two groups, the OS at five years of mLVRH and TLRH were 93.6% and 77.6% ï¼P=0.007ï¼. â¡ Histologic subtypes: for the OS at five years of squamous-cell carcinoma, mLVRH and TLRH were 96.1% and 92.3%, and there was significant difference ï¼P=0.046ï¼ï¼ for adenocarcinoma, the OS at five years were 91.0% and 88.6%ï¼ and there was no difference between two groups ï¼P=0.230ï¼. ⢠Lymph node metastasisï¼ the mLVRH and TLRH with lymph node metastasis, the OS at five years were 98.6% and 96.4%; the mLVRH and TLRH without lymph node metastasis, the OS at five years were 89.3% and 80.8%. There were no significant differences between the two groups,respectively (P=0.156ï¼ P=0.093ï¼. (2) The DFS at 5 years: there was no significant difference between mLVRH and TLRH (94.1% vs 90.9%, P=0.220). Stratify analysis for stage of disease, the mLVRH group was higher than that in the TLRH group in stage â b1 (97.0% vs 92.8%, P=0.039). However, for stage â ¡a1, there was no significant difference between mLVRH and TLRH group ï¼88.2% vs 75.8%, P=0.074ï¼. Conclusions: The results of this retrospective study indicated that different laparoscopy surgical procedures had diverse oncologic outcomes. The OS at 5 years of the mLVRH is superior to the TLRH. The DFS at 5 years in â b1 stage, the mLVRH is higher than the TLRH. Therefore, the modified laparoscopy is still an alternative surgery for early cervical cancer patients when following the principle of no-tumor-exposure.
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Histerectomia/métodos , Laparoscopia/métodos , Excisão de Linfonodo , Neoplasias do Colo do Útero/cirurgia , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Estudos Retrospectivos , Resultado do Tratamento , Neoplasias do Colo do Útero/patologiaRESUMO
Anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) is usually a multisystem disorder, and pulmonary renal syndrome is a common presentation. Patients with AAV are less likely to experience relapse when they progress to end-stage renal disease (ESRD). We report a rare case of diffuse alveolar hemorrhage (DAH) in relapsing AAV after eight years of haemodialysis. A 58-year-old woman was admitted to our hospital with the chief complaints of dyspnea and hemoptysis accompanied by anemia, fever, fatigue, and weight loss. She had elevated anti-myeloperoxidase (MPO) titer. The computer tomoghraphy showed diffuse alveolar hemorrhage. After the recurrent episode of AAV was diagnosed, she underwent the following therapy: Plasmapheresis was initiated within 24 h after admission, 3 000 mL of plasma was removed per session, and the anticoagulation of citrate was applied during plasmapheresis. Five plasmapheresis treatments were performed, and after three apheresis sessions, the pulmonary hemorrhage ceased. Other treatments included a methylprednisolone bolus, tapered to oral prednisone and cyclophosphamide. Regular hemodialysis was scheduled. These treatments resulted in resolution of the inflammatory symptoms, DAH improved. Her anti-MPO level decreased. The patient was discharged in good condition. AAV with DAH is usually acute at the onset and is generally a condition with high morbidity and substantial mortality. Therefore, prompt diagnosis and aggressive treatment are needed to improve survival.
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Vasculite Associada a Anticorpo Anticitoplasma de Neutrófilos , Hemorragia , Pneumopatias , Diálise Renal , Vasculite Associada a Anticorpo Anticitoplasma de Neutrófilos/etiologia , Anticorpos Anticitoplasma de Neutrófilos , Feminino , Hemorragia/etiologia , Hemorragia/imunologia , Humanos , Pneumopatias/etiologia , Pneumopatias/imunologia , Pessoa de Meia-Idade , Alvéolos Pulmonares , Diálise Renal/efeitos adversosRESUMO
Ovarian cancer is the major gynaecologic malignancy and the leading cause of death in gynaecological cancer. Heat shock proteins (HSPs) are highly expressed in many malignant cancers and involved in metastasis including ovarian cancer. The early detection of peritoneal metastases in epithelial ovarian cancer may be more important in clinical care. HSP27, a small heat shock protein, is correlated with peritoneal metastases in epithelial ovarian cancer tissues. In this study, we investigated whether the levels of total HSP27 were detectable in serum and whether it could be a predictive biomarker for peritoneal metastases in epithelial ovarian cancer. Serum samples from 48 patients with epithelial ovarian cancer, 35 patients with benign ovarian tumours and 24 healthy women were included in this study. The serum levels of total HSP27 were measured by enzyme-linked immunosorbent assay (ELISA). There was no difference in the serum levels of total HSP27 between women with benign ovarian tumours and healthy women. However, the serum levels of total HSP27 were significantly increased in patients with epithelial ovarian cancer. The increased serum levels of total HSP27 were only seen in patients with peritoneal metastases. Furthermore, increased serum levels of total HSP27 were significantly reduced after the combination chemotherapies in patients with peritoneal metastases. These data suggest that circulating HSP27 levels were increased in epithelial ovarian cancer and correlated with peritoneal metastases. The measurement of serum HSP27 levels may be used as a potential additional indicator for peritoneal metastases in epithelial ovarian cancer and response to treatment.
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Proteínas de Choque Térmico HSP27/sangue , Neoplasias Epiteliais e Glandulares/sangue , Neoplasias Ovarianas/sangue , Neoplasias Peritoneais/sangue , Adulto , Idoso , Biomarcadores Tumorais/sangue , Carcinoma Epitelial do Ovário , Ensaio de Imunoadsorção Enzimática , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Neoplasias Peritoneais/secundárioRESUMO
Ovarian cancer is the leading cause of death in gynaecological cancers. The high temperature requirement factor A3 (HtrA3) is involved in the pathogenesis of ovarian cancer. In this study we investigated whetherHtrA3 protein levels were altered in subtypes of ovarian cancer and whether HtrA3 down-regulation was associated with peritoneal metastasis. Ovarian cancer tissues from 89 patients were analyzed by immunohistochemistry. The levels of HtrA3 protein were lower in all subtypes of ovarian cancer and the lowest levels of HtrA3 were in epithelial ovarian cancer. The down-regulation of HtrA3 levels was not correlated with peritoneal metastasis of epithelial ovarian cancer.
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Regulação para Baixo , Neoplasias Ovarianas/metabolismo , Serina Endopeptidases/metabolismo , Adolescente , Adulto , Idoso , Carcinoma Epitelial do Ovário , Feminino , Humanos , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Neoplasias Epiteliais e Glandulares/metabolismo , Neoplasias Epiteliais e Glandulares/patologia , Neoplasias Ovarianas/patologia , Adulto JovemRESUMO
It is known that aberrant sialylation of IgA1 is involved in the pathogenesis of IgA nephropathy (IgAN). We hypothesize that aberrant sialylation of serum IgA1 may result from changes in the activity of alpha2,6-sialyltransferase (alpha2,6-ST) or expression of its coding gene ST6GALNAC2 in peripheral B lymphocytes. Sixty patients with IgAN and 20 healthy controls were enrolled. Peripheral B lymphocytes were isolated by CD-19-positive magnetic beads. The expression level of ST6GALNAC2 was quantitatively analysed by real-time reverse-transcriptase polymerase chain reaction (PCR). Serum IgA1 and sialylation levels were detected by enzyme-linked immunosorbent assay (ELISA) and specific lectin-binding ELISA. Activity of alpha2,6-ST was measured by specific lectin-binding ELISA. Expression of ST6GALNAC2 in B peripheral lymphocytes was significantly lower in patients with IgAN than that in normal controls (3.7 +/- 2.2 versus 6.3 +/- 2.3, P = 0.016); alpha2,6-ST activity in B lymphocytes was correlated positively with the level of alpha2,6-sialic acid in serum IgA1 in patients (n = 42) and controls (n = 12) (r = 0.37, P = 0.007). However, alpha2,6-ST activity did not differ between patients with IgAN and controls (1.19 +/- 1.43 versus 1.06 +/- 1.17, P > 0.05). These data suggested that reduced sialylation of serum IgA1 may result from decreased expression of ST6GALNAC2. The factors affecting activity of alpha2,6-ST in the sialylation of IgA1 need to be further investigated.
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Linfócitos B/enzimologia , Glomerulonefrite por IGA/enzimologia , Sialiltransferases/genética , Sialiltransferases/metabolismo , Adulto , Feminino , Glicosilação , Humanos , Imunoglobulina A/sangue , Imunoglobulina A/metabolismo , Masculino , RNA Mensageiro/análise , beta-D-Galactosídeo alfa 2-6-SialiltransferaseRESUMO
OBJECTIVE: MicroRNAs are noncoding RNAs which are involved in the occurrence and progression of tumors. This study aims to explore the role of microRNA-92a in cutaneous malignant melanoma (CMM) and its underlying mechanism. PATIENTS AND METHODS: The expression level of microRNA-92a in 75 pairs of CMM tissues and paracancerous tissues was determined using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between microRNA-92a expression with clinical data of CMM patients was analyzed. Besides, microRNA-92a expression in CMM cells and primary epidermal melanocytes (PEM) was determined by qRT-PCR as well. After transfection of si-microRNA-92a in CMM cells, biological performances of CMM were determined using cell counting kit-8 (CCK-8), colony formation and transwell assay, respectively. FOXP1 expression in CMM cells and tissues was determined using Western blot. Kaplan-Meier survival curves were drawn to explore the correlation between the FOXP1 expression and prognosis of CMM patients. RESULTS: MicroRNA-92a was highly expressed in CMM tissues compared with that of paracancerous tissues. Compared with CMM patients with lower expression of microRNA-92a, those with higher expression of microRNA-92a presented higher tumor stage, higher incidences of lymph node metastasis and distant metastasis, as well as lower overall survival. The knockdown of microRNA-92a remarkably decreased proliferative, invasive and metastatic capacities of CMM cells. Western blot results elucidated that microRNA-92a knockdown in CMM cells upregulates FOXP1 expression. Additionally, rescue experiments showed that mi-croRNA-92a regulates biological performances of CMM cells by regulating FOXP1. CONCLUSIONS: MicroRNA-92a is highly expressed in CMM, which is remarkably correlated to tumor stage and poor prognosis of CMM patients. We found that microRNA-92a pro-motes malignant progression of CMM by regulating FOXP1.
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Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Melanoma/patologia , MicroRNAs/genética , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Neoplasias Cutâneas/patologia , Regiões 3' não Traduzidas , Linhagem Celular Tumoral , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Melanoma/genética , Melanoma/metabolismo , Estadiamento de Neoplasias , Prognóstico , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/metabolismo , Regulação para Cima , Melanoma Maligno CutâneoRESUMO
Fires resulting from titanium combustion are complex and violent processes which can instantly burn a titanium alloy once ignited. The occurrence of titanium combustion is a disaster for aircraft. Accurate measurement of the ignition temperature of titanium alloys is of significance in preventing such fires and in investigating combustion-resistance properties. In this study, monochromatic temperature and emissivity measurement methods based on reflectivity detection were used to determine the ignition temperature of a titanium alloy. Experiments were carried out using a titanium burning apparatus. The temperatures of titanium in the oxidation stage before ignition and in the combustion stage during the ignition process were measured using wavelengths of 1050 nm and 940 nm, respectively. Experimental results showed that the ignition temperature of the titanium alloy could be measured by reflectivity detection and that measurement precision during thermal oxidation (500-900 °C) was ±1 °C. The temperature of the ignition process ranged between 1653 and 1857 °C, and the ignition temperature was around 1680 °C.
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Whitmania pigra whitman is one of the traditional Chinese drugs commonly used in clinical practice for Blood circulation promoting and Stasis relieving, its active principle was unknown yet. In this report, five extracting methods have been studied based on its extract's anticoagulant and antithrombotic activity in vitro. The results have revealed that the extracts using different extracting methods showed different anticoagulant and anti-thrombotic activity. Among them, the extracts produced by water-boiling and alcohol-prepitating have shown the strongest anticoagulant activity and good antithrombotic effect.
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Sanguessugas , Materia Medica/farmacologia , Animais , Anticoagulantes/farmacologia , Fibrinolíticos/farmacologia , Sanguessugas/química , Masculino , Coelhos , Tecnologia Farmacêutica , Tempo de TrombinaRESUMO
OBJECTIVE: To study the protective effect for Chinese medicine of promoting blood circulation and removing blood stasis (PBCRBS) on vascular endothelial cells (VEC) in rats, aiming at further research on the mechanism of blood stasis syndrome and PBCRBS. METHOD: Establishing a model of blood stasis with endothelial damage by means of giving rats an injection of adrenalin and making it swim in ice-cold water, then measuring the number of circulating endothelial cells (CEC) in whole blood and rheology. Moreover observing the change of the above indexes after the rats have taken Leech, Radix salviae miltrorrhizae (RSM) and its composite recipe for 5 days or 10 days. RESULT: It could increase the number of CEC accompanied by whole blood viscosity, fibrinogen and hematocrit in rats with blood stasis. But for groups taking Chinese medicine, the number of CEC was decreased distinctively and blood rheology was improved. CONCLUSION: The rat with blood stasis syndrome suffered from obvious injury of VEC. The Leech, RSM and its composite recipe could protect VEC.
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Medicamentos de Ervas Chinesas/farmacologia , Endotélio Vascular/efeitos dos fármacos , Sanguessugas , Medicina Tradicional Chinesa , Salvia miltiorrhiza , Animais , Contagem de Células , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/isolamento & purificação , Endotélio Vascular/citologia , Feminino , Hemorreologia/efeitos dos fármacos , Sanguessugas/química , Masculino , Materia Medica/isolamento & purificação , Materia Medica/farmacologia , Plantas Medicinais/química , Ratos , Ratos Wistar , Salvia miltiorrhiza/químicaRESUMO
The pathogenesis of preeclampsia is not clear but the disease is characterised by systemic endothelial cell dysfunction that is considered to be triggered by a placental factor. Necrotic trophoblastic debris that is deported in the maternal blood is one possible placental trigger for preeclampsia. Syncytial knots were first associated with preeclampsia over 100 years ago. However, syncytial knots are very large and most are trapped in the pulmonary capillaries making it difficult to envisage how they could lead to widespread systemic endothelial cell dysfunction. This study was undertaken to examine whether conditioned medium from endothelial cells that have phagocytosed necrotic trophoblastic debris could adversely affect the proliferation or survival of fresh endothelial cells. Trophoblastic cellular debris, harvested from placental explants was added to endothelial cell monolayers directly or after induction of necrosis by freeze-thawing. Conditioned medium from the endothelial cell cultures was exposed to fresh endothelial cells and their proliferation measured by Alamar Blue, and CyQUANTNF cell proliferation assays. Endothelial cell death was examined by a fluorogenic caspase-3 activity assay and LDH release. Conditioned medium from endothelial cells that had phagocytosed necrotic but not apoptotic trophoblastic debris significantly inhibited the proliferation of fresh endothelial cells but did not induce their death. The conditioned medium also reduced cell-surface endoglin expression by fresh endothelial cells. These results confirm that phagocytosis of necrotic trophoblastic debris by endothelial cells results in the secretion of soluble factors that might explain how necrotic trophoblastic debris trapped in the pulmonary capillaries could induce systemic endothelial cell dysfunction.
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Comunicação Celular , Proliferação de Células , Células Endoteliais/fisiologia , Endotélio Vascular/fisiopatologia , Fagocitose , Pré-Eclâmpsia/etiologia , Trofoblastos , Antígenos CD/metabolismo , Linhagem Celular , Membrana Celular/metabolismo , Sobrevivência Celular , Meios de Cultivo Condicionados , Citocinas/metabolismo , Endoglina , Endotélio Vascular/metabolismo , Feminino , Congelamento , Humanos , Microvasos/metabolismo , Microvasos/fisiopatologia , Necrose , Técnicas de Cultura de Órgãos , Placenta/química , Placenta/patologia , Pré-Eclâmpsia/metabolismo , Pré-Eclâmpsia/fisiopatologia , Gravidez , Receptores de Superfície Celular/metabolismo , Trofoblastos/químicaRESUMO
Recombinant thanatin analog (TH1) is a cationic 20-amino-acid antibacterial peptide with a conserved cysteine disulfide bond. It exhibits a broad antibacterial spectrum. Different strategies have been developed to produce small antibacterial peptides using recombinant techniques. To date, no efforts to obtain large quantities of active recombinant TH1 have been reported. This study describes the synthesis of TH1 gene, the heterologous fusion expression of the peptide in Escherichia coli, and the bioactive assay of released TH1. By constructing the expression plasmid (pET32a-TH1), high yields of soluble TH1 fusion protein (0.416 g/L) can be obtained in E. coli. Further optimization studies have been carried out to increase the expression of TH1 in different culture conditions, with the final amount of pure TH1 being 13.2 mg/L. The results show that the expression system provides a simple and reliable strategy for generating large quantities of TH1 by soluble fusion expression in E. coli.