[Correlation between first tarsometatarsal joint sagittal mobility and hallux valgus combined with transfer metatarsalgia].

Objective: To investigate the correlation between the first tarsometatarsal joint (TMT1) sagittal mobility and hallux valgus (HV) combined with transfer metatarsalgia (TM). Methods: The weight-bearing CT (WBCT) imaging data of 111 HV patients (167 feet) who were treated at the Foot and Ankle Surgery Center, Beijing Tongren Hospital Affiliated to Capital Medical University from January 2018 to January 2020 were retrospectively analyzed. The patients underwent PedCAT WBCT scans of both feet, and the hallux valgus angle (HVA), intermetatarsal angle (IMA), plantar distance (PD), medial cuneiform-first metatarsal angle (CMA) and metatarsal protrusion distance (MPD) were measured using CubeVue software. PD and CMA were signs of TMT1 instability. Visual analogue scale (VAS) and foot and ankle ability measures (FAAM) scores were obtained. The patients were divided into TM group and non-TM group according to the presence of metatarsalgia. The TM group and the non-TM group were compared in terms of HVA, IMA, PD, CMA, MPD, VAS and FAAM. Correlations between PD, CMA and HVA, IMA, VAS, FAAM were analyzed using Spearman correlation. Results: Total of 111 cases were included in this study, there were 35 males and 76 females with a mean age of (57.7±14.1) years. The average values of HVA (37.9°±8.6°), IMA (17.9°±2.6°), CMA (2.1°±0.3°) and PD [(1.8±0.4) mm] in TM group were all significantly higher than those in the non-TM group [HVA (32.5°±9.1°), IMA (15.1°±3.4°), CMA (1.7°±0.3°) and PD (1.6±0.2) mm] (All P<0.001). There was no significant difference in MPD between the two groups (P=0.580). The TM group demonstrated a higher VAS score when compared with the non-TM group (P<0.001). The FAAM score of the TM group (54.1±11.8) was significantly lower than that in the non-TM group (66.2±11.4) (P<0.001). The results of Spearman correlation analysis showed that there was no correlation between PD and HVA, IMA and VAS score. There was a negative correlation between PD, CMA and FAAM score, and the difference was statistically significant (rs=-0.637, -0.254, both P<0.001); CMA was positively correlated with HVA, IMA, and VAS score (rs=0.603, 0.971, 0.269, all P<0.001). Conclusions: WBCT is helpful for the diagnosis of TMT1 sagittal instability. The severity of TMT1 sagittal instability is positively correlated with hallux valgus and TM. The TMT1 instability may play an important role in the pathogenesis of hallux valgus.

First Report of Boll Rot of Cotton Caused by Pantoea agglomerans in China.

Since the summer of 2006, bacterial boll rot of cotton has been observed on fruits of 'Xinluzao 31' (Xinluzao 6 × Acala) in Xinjiang Province. It resulted in as much as 20% yield loss in several fields. Symptoms do not appear on the outer carpel. In the infected cotton bolls, fibers do not mature completely and seed tissue exhibits brown necrotic coloration. Lint and seeds from 24 surface-disinfested cotton bolls were triturated and plated onto King's medium B (KB). Plates were incubated at 28°C for 48 h. Forty eight strains with yellow pigmentation on KB were characterized. All were nonfluorescent on KB, gram negative, facultatively anaerobic, unable to produce indole from tryptophan, able to reduce nitrate to nitrite, and produce acid from glucose, cellobiose, lactose, melibiose, and melonate. In addition, 16S rDNA in seven strains was amplified with universal primers (1). The PCR products were cloned into pGEM-T easy vector and sequenced. A BLAST search of the seven sequences against the GenBank nucleotide library indicated 100% identity with the 16S rDNA sequence of Enterobacter agglomerans strain A80. Then an additional primer pair, pagF and pagR (3), was used for more specific amplification of Pantoea agglomerans 16S rDNA, which resulted in single highly specific fragments of approximately 1 kb. On the basis of morphological, physiological, biochemical characteristics, and 16S rRNA gene sequence analysis, we identified the bacterium to be P. agglomerans. To confirm pathogenicity, cell suspensions (1 × 108 CFU/ml) of eight representative strains were used to inoculate cotton at peak bolling stage in the field. Cell suspensions, or water as the control, were applied to stigma scars, wall sutures, and scratch wounds on bracts, calyxes, and bolls. Alternatively, a needle was used to puncture through a drop of suspension placed on the boll wall suture and bracts. At least 20 bolls or flowers were inoculated with each bacterial strain per inoculation method. Infection occurred only when bacterial injections breached the endocarp of the boll either through the carpel wall or a suture between carpel sections. Disease symptoms developed 1 week postinoculation. The inoculated organism was reisolated from the diseased tissues. P. agglomerans is generally regarded to be a soil saprophyte or leaf epiphyte, but strains can opportunistically infect plants triggering gall formations or human wounds causing septic arthritis. The disease symptoms and pathogen characteristics observed in this study are identical to those reported in the United States (2). To our knowledge, this is the first report of P. agglomerans causing boll rot of cotton in China. References: (1) S. Manulisi and I. Barash. Mol. Plant Pathol. 4:307, 2003. (2) E. G. Medrano et al. J. Appl. Microbiol. 103:436, 2007. (3) S. Vorwerk et al. Agric. For. Entomol. 9:57, 2007.

A protocol for manual segmentation of medial temporal lobe subregions in 7 Tesla MRI.

Recent advances in MRI and increasing knowledge on the characterization and anatomical variability of medial temporal lobe (MTL) anatomy have paved the way for more specific subdivisions of the MTL in humans. In addition, recent studies suggest that early changes in many neurodegenerative and neuropsychiatric diseases are better detected in smaller subregions of the MTL rather than with whole structure analyses. Here, we developed a new protocol using 7 Tesla (T) MRI incorporating novel anatomical findings for the manual segmentation of entorhinal cortex (ErC), perirhinal cortex (PrC; divided into area 35 and 36), parahippocampal cortex (PhC), and hippocampus; which includes the subfields subiculum (Sub), CA1, CA2, as well as CA3 and dentate gyrus (DG) which are separated by the endfolial pathway covering most of the long axis of the hippocampus. We provide detailed instructions alongside slice-by-slice segmentations to ease learning for the untrained but also more experienced raters. Twenty-two subjects were scanned (19-32 yrs, mean age = 26 years, 12 females) with a turbo spin echo (TSE) T2-weighted MRI sequence with high-resolution oblique coronal slices oriented orthogonal to the long axis of the hippocampus (in-plane resolution 0.44 × 0.44 mm2) and 1.0 mm slice thickness. The scans were manually delineated by two experienced raters, to assess intra- and inter-rater reliability. The Dice Similarity Index (DSI) was above 0.78 for all regions and the Intraclass Correlation Coefficients (ICC) were between 0.76 to 0.99 both for intra- and inter-rater reliability. In conclusion, this study presents a fine-grained and comprehensive segmentation protocol for MTL structures at 7 T MRI that closely follows recent knowledge from anatomical studies. More specific subdivisions (e.g. area 35 and 36 in PrC, and the separation of DG and CA3) may pave the way for more precise delineations thereby enabling the detection of early volumetric changes in dementia and neuropsychiatric diseases.

Revisiting the role of erythropoietin for treatment of ocular disorders.

Erythropoietin (EPO) is a glycoprotein hormone conventionally thought to be responsible only in producing red blood cells in our body. However, with the discovery of the presence of EPO and EPO receptors in the retinal layers, the EPO seems to have physiological roles in the eye. In this review, we revisit the role of EPO in the eye. We look into the biological role of EPO in the development of the eye and the physiologic roles that it has. Apart from that, we seek to understand the mechanisms and pathways of EPO that contributes to the therapeutic and pathological conditions of the various ocular disorders such as diabetic retinopathy, retinopathy of prematurity, glaucoma, age-related macular degeneration, optic neuritis, and retinal detachment. With these understandings, we discuss the clinical applications of EPO for treatment of ocular disorders, modes of administration, EPO formulations, current clinical trials, and its future directions.

Inferior parietal lobule projections to the presubiculum and neighboring ventromedial temporal cortical areas.

The entorhinal and perirhinal cortices have long been accorded a special role in the communications between neocortical areas and the hippocampal formation. Less attention has been paid to the presubiculum, which, however, is also a component of the parahippocampal gyrus, receives dense inputs from several cortical areas, and itself is a major source of connections to the entorhinal cortex (EC). In part of a closer investigation of corticohippocampal systems, the authors applied single-axon analysis to the connections from the inferior parietal lobule (IPL) to the presubiculum. One major result from this approach was the finding that many of these axons (at least 10 of 14) branch beyond the presubiculum. For 4 axons, branches were followed to area TF and to the border between the perirhinal and entorhinal cortices, raising the suggestion that these areas, which sometimes are viewed as serial stages, are tightly interconnected. In addition, the current data identify several features of presubicular organization that may be relevant to its functional role in visuospatial or memory processes: 1) Terminations from the IPL, as previously reported for prefrontal connections (Goldman-Rakic et al. [1984] Neuroscience 12:719-743), form two to four patches in the superficial layers. These align in stripes, but only for short distances ( approximately 1.5 mm). This pattern suggests a strong compartmentalization in layers I and II that is also indicated by cytochrome oxidase and other markers. 2) Connections tend to be bistratified, terminating in layers I-II and deeper in layer III. 3) Single axons terminate in layer I alone or in different combinations of layers. This may imply some heterogeneity of subtypes. 4) Individual axons, both ipsilateral projecting (n = 14 axons) and contralateral projecting (n = 6 axons), tend to have large arbors (0.3-0.8 mm across). Finally, the authors observe that projections from the IPL, except for its anteriormost portion, converge at the perirhinal-entorhinal border around the posterior tip of the rhinal sulcus. These projections partially overlap with projections from ventromedial areas TE and TF, and this convergence may contribute to the severe deficits in visual recognition memory resulting from ablations of rhinal cortex.

A modification of biotinylated dextran amine histochemistry for labeling the developing mammalian brain.

Biotinylated dextran amine (BDA) has proven to be an excellent anterograde tracer in adult mammalian brains, having some advantages over other anterograde tracers such as Phaseolus vulgaris-leucoagglutinin (PHA-L) and biocytin. However, results are inferior when BDA is used in neonatal mammals. To improve the sensitivity and quality of BDA labeling in neonatal mammalian brains, the tetramethylbenzidine-sodium tungstate (TMB-ST) method for horseradish peroxidase (HRP) histochemistry was modified and used in BDA histochemistry. After BDA application to the visual cortex of neonatal rat and cat, contralateral and ipsilateral cortical and subcortical regions were examined for BDA-labeled exons and terminals. The modified BDA histochemistry produced corpus callosum (CC) axons in neonatal rat and cat that were heavily and continuously labeled. The distribution, trajectories, branching and termination of individual CC axons, and even possible axon-axon contracts, were clearly identified in exquisite detail, even at low magnification. The quality of BDA labeling in the ipsilateral lateral geniculate nucleus and superior colliculus was similar to that of the CC axonal labeling. These results indicate that the modified BDA histochemistry provides a very sensitive and reliable approach to revealing the detailed distribution and morphology of projecting axons and terminals in the developing mammalian nervous system.

Neuropeptide Y- and somatostatin-immunoreactive axons in the corpus callosum during postnatal development of the rat.

Corpus callosum (CC) projections in adult mammals were generally thought to be excitatory and to use excitatory amino acids as their transmitters. Little information has been available about the electrical properties and neurochemical status of developing CC connections. The present study investigated the chemical status of rat CC axons during postnatal development by using antibodies to neuropeptide Y (NPY) and to somatostatin (SOM). Both NPY-immunoreactive (ir) and SOM-ir axons were found in the CC of the rat from newborn through adult; however, the number of SOM-ir CC axons is less than that of NPY-ir CC axons at corresponding ages. The density of both NPY-ir and SOM-ir CC axons initially increased, then peaked, and finally decreased to the mature level. In the adult, only a few NPY-ir and SOM-ir CC axons were found in the CC. These results indicate that many NPY-ir and SOM-ir CC axons are transitory during early postnatal development. The results also suggest that the functions of CC connections in adult mammals may be different from that of developing ones. The present results as well as the previous results demonstrate that both developing and mature CC connections are chemically heterogeneous.

Confirmation of the existence of transitory corpus callosum axons in area 17 of neonatal cat: an anterograde tracing study using biotinylated dextran amine.

Corpus callosum (CC) axons in visual cortex were labeled anterogradely by in vivo biotinylated dextran amine (BDA) in neonatal cat at postnatal day (PND) 6, 10 and 15. Labeled CC axons were distributed throughout the visual cortex including medial area 17. The number of CC axons in medial area 17 increased from PND 6 to PND 10, and then decreased from PND 10 to PND 15. At PND 15, few CC axons could be followed into the grey matter in medial area 17. Thus, BDA labels transitory CC axons that extend through all cortical layers in medial area 17, confirming the results revealed by in vitro DiI labeling.

Neuropeptide Y immunoreactive axons in the corpus callosum of the cat during postnatal development.

Many immunocytochemical studies have identified different types of neurotransmitters localized in the corpus callosum (CC) axons in the adult mammal. Few studies have looked at the development of different neurochemically identified CC systems. Previous studies on the development of cat CC axons have indicated that a large number of transitory CC axons project to the cortex during early postnatal development. The present study focuses on the development of one neurochemically identified group of CC axons in the cat, labeled with an antibody against neuropeptide Y (NPY), to determine if this group participates in transitory CC axonal growth. Cats at specified ages from birth to adulthood were studied with a routine method of immunocytochemistry for antiserum to NPY. NPY-immunoreactive (ir) CC axons were detected at all stages examined, from newborn to adult; the peak density occurred during postnatal weeks (PNW) 3-4. During PNW 1-2, the density of NPY-ir CC axons increased gradually; some NPY-ir axons at this age had growth cones located within the CC bundle between the cerebral hemispheres. The density of the NPY-ir CC axons decreased gradually during PNW 5-7, and from PNW 8 to maturity only a few NPY-ir CC axons were observed. These results indicate that at least two types of NPY-ir CC axons (i.e., transitory and permanent) exist during development, and that most of these axons are eliminated or only express NPY-ir for a short period during development. The results also indicate that neurochemical subsets of CC axons participate in the extensive transitory growth observed by means of the membrane tracer DiI but they may follow unique developmental timetables.

Parvalbumin immunoreactive Cajal-Retzius and non-Cajal-Retzius neurons in layer I of different cortical regions of human newborn.

Neurons of layer I play an important role in the development of the basic structural and functional organization of the mammalian cerebral cortex. Basic data, however, concerning the spatial and temporal distribution of the neuron populations in layer I are still limited, especially for human material. The present study investigates the distribution of Cajal-Retzius (CR) and non Cajal-Retzius (NCR) neurons in thirteen cortical areas in the newborn human in terms of their relative density and possible subtypes. Neuronal populations were identified by immunohistochemistry for parvalbumin. Three main results are reported. First, parvalbumin-immunoreactive (Parv-ir) CR cells were observed in all of the neocortical areas examined. These areas also had a Parv-ir horizontal fiber plexus in deep layer I, confirming to the horizontal plexus classically associated with CR neurons. Second, many Parv-ir CR cells showed clear signs of degeneration. Third, in addition to the large CR cells, smaller Parv-ir NCR neurons occurred in many of the neocortical areas examined. These were morphologically heterogeneous and may represent several subtypes. By sampling across several areas, we were able to establish that these NCR cells occurred at higher density in primary sensory areas 3, 1, 17, and 41. Because of this variability in density of Parv-ir NCR cells, the ratio of Parv-ir CR to Parv-ir NCR cells is selectively lower in primary sensory areas. Recent investigations in somatosensory cortex of early postnatal rat report complex spatiotemporal patterns of correlated spontaneous activity among neurons in layer I (Schwartz et al. 1998). An interesting possibility is that regional variability in this activity may play a major role in the organization of cortical circuitry in different areas.

Oviduct occlusion by intraluminal thread and silver clips.

PIP: 100 women ranging in age from 26-34 years were followed up for 4-21 months after undergoing sterilization by tubal occlusion using intraluminal threads and silver clips. The number of children/woman ranged from 1-5 with 80% having 2. 31 women underwent sterilization postmenstrually, 11 midmenstrually, 28 premenstrually, 12 after abortion, and 8 during lactation. The timing was unknown in 8 cases. The silver clips used were hook eye "db" shaped, 3 mm wide, .5 mm thick, and 70, 100, or 150 mg in weight. Local anesthesia, suprapubic small abdominal incision, and oviduct delivery were the usual procedures. The clips were placed where the tube is relatively straight and avascular, often near the isthmus or ampulla. No. 4 nylon thread was drawn through the tube and a silver clip was put on each tube and tightened until the threads could no longer be moved. The extra thread was snipped off about 3 mm from the clip. There were no pregnancies among 95 patients followed up. 18 patients had varying degrees of lumbago or leg pain and 1 complained of serious lower abdominal pain, especially after coitus. Pelvic examination in 74 patients showed 1 case of slight thickening in the right appendix region but no pelvic masses. Hysterosalpingography in 22 patients showed 1 case with incomplete occlusion on 1 side. The method is believed to be safe and suitable for use in smaller hospitals and outpatients. Reversibility has been demonstrated in rabbits.^ieng

[Estimation of case fatality rate and incidence rate of traffic injury in Taiwan--analysis of 4,329 victims at a medical center].

Accidents remain the third leading cause of mortality in Taiwan, of which traffic injuries constitute the major part. In order to characterize traffic injuries and to estimate the annual traffic injury rate in Taiwan, we conducted an epidemiologic study of 4,329 consecutive traffic accident victims, managed at the Tri-Service General Hospital from January to December of 1990. Victims were interviewed for demographic data and types of accident using a brief questionnaire. The questions included injury site and Injury Severity Score (ISS); final diagnosis and outcome were obtained by review of medical records and by a telephone survey performed 4 months after discharge. The sex- and age-specific case fatality rates calculated from the data obtained in this study were used to divide the annual incidence of traffic mortality abstracted from the vital statistics of Taiwan (1990) and, thus, to obtain an annual frequency of traffic injury cases under certain assumptions. The results showed that more men than women were injured in all age groups. The highest frequency of traffic injuries was noted in patients ranging in age from 20 to 39 years. Up to 58.9% of the victims were motorcycle users, followed by pedestrians (19.5%) and automobile-users (16.0%). The case fatality rate was 2.5% for males and 1.7% for females. The estimated annual traffic incidence was about 330,000, and the total annual rate of traffic injuries in Taiwan was 1,627/100,000 (2,188/100,000 for men and 1,114/100,000 for women).(ABSTRACT TRUNCATED AT 250 WORDS)

[Head injuries in traffic accidents with emphasis on the comparisons between motorcycle-helmet users and non-users].

Motor-vehicle incidents in Taiwan are a major cause of head injuries. Our objective was to analyze the characteristics of head injuries caused by motor vehicle accidents in terms of the injured person's demographic characteristics, time and types of crash, injury severity on the abbreviated injury scale (AIS), medical cost and benefits of helmet protection. For this purpose we conducted an epidemiologic survey of 2451 consecutive victims of traffic accidents, coming to, or managed at, the emergency care department of a large Taipei metropolitan hospital in 1990. The results showed that the most common cause of head injury in traffic accidents was a motorcycle incident. Motorcycle accident injured patients were generally young males, laborers or students. Most head injuries occurred between 16:00 and 23:00, and peaking at 21:00 in a day. Forty-three points four percent of patients were admitted, major head injury fatalities occurred within one week of the collision. The elderly or patients with high AIS comprised most nonsurvivors. According to our data, helmet usage was about 14% overall. Helmetless young riders were numerous among the total victims. A significant difference was noted in the severity of injury of helmeted users of motorcycles, compared with those who wore no helmet. As expected, patients who did not wear a helmet had a greater AIS average and higher rate of fatality. Forty-one of the 42 fatalities were patients who had been riding on motorcycles without helmets. Helmets provide adequate protection and reduce severity of injury, and medical cost for motorcycle collisions.

[Non-Hodgkin's lymphoma in adults: a clinico-pathologic study of 169 cases].

From 1983 to 1988, non-Hodgkin's lymphoma was diagnosed in 169 patients ranging from 19 to 89 years of age (mean 49). They were investigated using both unifactorial and multivariate regression (Cox model) analyses to determine the relationship of survival rate and prognostic factors including age, sex, histology, B symptom and clinical stage. All cases were histologically classified according to the criteria of the working formulation and were staged according to the Ann Arbor classification. Non-Hodgkin's lymphoma occurred roughly 9 times as frequently as Hodgkin's disease. The lymph nodes of the neck and inguinal regions were noted to be the most common sites of involvement. However, extranodal lymphomas originated most frequently from the stomach. For the patients with non-Hodgkin's lymphoma, the proportions of low grade, intermediate grade, and high grade were 10.7%, 59.1% and 20.7%, respectively. The remaining 9.5% of cases were unclassified (7.7%) and true histiocytic (1.8%). The most common subtype was diffuse large cell (33.7%). Lymphoblastic lymphoma was found to have a predilection for young male adults. Follicular lymphomas occurred mainly in mid-adult life. The patients with high grade lymphoma almost always presented the advanced stage. The median survival time (MST) of our series was 20 months, the 3-year survival rate was 43.3% and the 5-year survival rate was 30.40%. Statistical studies of both unifactorial (p less than 0.01) and multivariate regression (p less than 0.05) analyses showed that patients with an age greater than 60 years old, having B symptom, clinically advanced stage, or both histologically intermediate and high grade experienced a much poorer prognosis.(ABSTRACT TRUNCATED AT 250 WORDS)

[Survival of lung cancer patients of different histologic types].

The survival curves of primary lung cancers are significantly different by histologic type. The purpose of this study was to analyze the survival curves and prognostic factors of primary lung cancers according to the histologic type at the Tri-Service General Hospital for the years from 1983 to 1988. All records of new patients admitted to TSGH with primary lung malignancies were retrieved from the tumor registry. The survival curve was estimated by Kaplan-Meier Limit Estimate. The prognostic significance of 6 clinical and pathologic factors (sex, age, stage of disease, primary location, histologic differentiation and treatment) were analyzed by single variable analysis and by Cox multivariate regression. There were 448 male and 199 female patients with a mean age of 61.5 years. Adenocarcinoma was the most common histologic type found in both sexes, 37.9% in the men and 72.8% in the women. Patients in the advanced stage with distant metastasis comprised 52.8%. The most frequent location was the upper lobe (37.8%). Methods of treatment were: a) no therapy (39.7%), b) radiotherapy (34.7%), c) surgery with radiotherapy (8.7%), and d) other treatment (16.9%). The median survival of the 647 patients was 6.8 months after diagnosis. The overall one-year, three-year, and five-year survival rates were 26%, 10% and 5%, respectively. Survival curves according to the histopathologic type demonstrated that patients with small cell carcinoma had the lowest cumulative survival and patients with adenocarcinoma had a lower cumulative survival than those with squamous cell carcinoma.(ABSTRACT TRUNCATED AT 250 WORDS)

[Studies on chemical constituents of Gynostemma compressum].

Four new saponins, gycomoside I-IV, were isolated from the aerial part of Gynostemma compressum X.X. Chen et D.R. Liang. The structures were elucidated on the basis of spectral and chemical evidences as 1 beta,3 beta,12 beta,20(S),26-pentahydroxy-dammar- 24(25)-en-20(S)-O-beta-D-glucopyranosyl1-6--beta-D-glucopyranoside (gycomoside I); 1 beta,3 beta,12 beta,20(S)-tetrahydroxy-dammar-24(25)-en-3-O- beta-D-glucopyranosyl-20(S)-O-beta-D-glucopyranosyl1-6--beta-D-glu copyranoside (gycomoside II); 1 beta,3 beta,12 beta,20(S), 26-pentahydroxy-dammar-24(25)-en-20(S)-O- beta-D-glucopyranoside (gycomoside III) and 1 beta,3 beta,12 beta,20(S),26-pentahydroxy-dammar-24(25)- en-3-O-beta-D-glucopyranosyl-20(S)-O-beta-D-glucopyranosyl1-6--bet a-D-glucopyranoside (gycomoside IV).

[Reactive histiocytosis variant of tuberculous lymphadenitis].

Paraffin blocks from 38 cases with superficial lymph nodes exhibiting reactive histiocytosis with debris-like necrosis and neutrophil infiltration were studied by means of polymerase chain reaction for amplifying the repetitive DNA sequence (123pb fragment) specific for mycobacterium tuberculosis (M.TB-PCR), BCG immunohistochemistry (BCG-IHC) as well as acid fast stain (AF) for M.TB/MOTT (mycobacterium other than tuberculosis). The comprehensive positive rate of the three methods was 52.6% (20/38 cases) and the separate positive rates for M.TB-PCR, BCG-IHC and AF being 50%, 26% and 0.8% respectively. The results prove that (1) half of the reactive histiocytosis of lymph nodes selected in this paper are related to tuberculosis infection, i.e., some tuberculous lymphadenitis may appear as a variant of reactive histiocytosis; and (2) PCR technique is a very valuable method for making the etiologic diagnosis of tuberculous lymphadenitis.

miR-874 regulates myocardial necrosis by targeting caspase-8.

Cardiomyocyte death is an important reason for the cardiac syndromes, such as heart failure (HF) and myocardial infarction (MI). In the heart diseases, necrosis is one of the main forms of cell death. MicroRNAs (miRNAs) are a class of small non-coding RNAs that mediate post-transcriptional gene silencing. Hitherto, it is not yet clear whether miRNA can regulate necrosis in cardiomyocyte. In this work, we performed a microarray to detect miRNAs in response to H2O2 treatment, and the results showed that miR-874 was substantially increased. We further studied the function of miR-874, and observed that knockdown of miR-874 attenuated necrosis in the cellular model and also MI in the animal model. We searched for the downstream mediator of miR-874 and identified that caspase-8 was a target of miR-874. Caspase-8 was able to antagonize necrosis. When suppressed by miR-874, caspase-8 lost the ability to repress necrotic program. In exploring the molecular mechanism by which miR-874 expression is regulated, we identified that Foxo3a could transcriptionally repress miR-874 expression. Foxo3a transgenic or knockout mice exhibited a low or high expression level of miR-874, and a reduced or enhanced necrosis and MI. Our present study reveals a novel myocardial necrotic regulating model, which is composed of Foxo3a, miR-874 and caspase-8. Modulation of their levels may provide a new approach for tackling myocardial necrosis.