Dynamics of internal isoprenoid metabolites in young Picea abies (Norway spruce) shoots during drought stress conditions in springtime.

Currently, large areas of Picea abies (Norway spruce) stands in Europe are increasingly affected by drought and heat waves. Moreover, early spring drought has occurred with much higher frequency. Our work focuses on physiological changes induced by drought in four-year-old spruce seedlings during shoot elongation. We investigated drought effect on photosynthetic rate, concentration of abscisic acid and its metabolites, amount and composition of monoterpenes in needles of seedlings from five different provenances (altitude range 550-1280 m above sea level) in Western Carpathians. Spruce seedlings subjected to one-month drought stress of moderate intensity (about 50% of soil water content at the end of experiment) showed significant reduction of CO2 uptake and increased concentration of ABA related to untreated controls. Induced drought affected needle monoterpene content and composition. Observed changes in drought-induced physiological parameters were influenced by seedling provenance. The provenance from 920 m above sea level showed the greatest sensitivity to drought with significantly highest ABA content and, at the same time, a clear decline of CO2 uptake and amounts of total monoterpenes. Our results indicating intra-specific provenance-related variability in physiological response of spruce seedlings to drought may provide a basis for improved reforestation strategies in drought risk areas.

The 2018 European heatwave led to stem dehydration but not to consistent growth reductions in forests.

Heatwaves exert disproportionately strong and sometimes irreversible impacts on forest ecosystems. These impacts remain poorly understood at the tree and species level and across large spatial scales. Here, we investigate the effects of the record-breaking 2018 European heatwave on tree growth and tree water status using a collection of high-temporal resolution dendrometer data from 21 species across 53 sites. Relative to the two preceding years, annual stem growth was not consistently reduced by the 2018 heatwave but stems experienced twice the temporary shrinkage due to depletion of water reserves. Conifer species were less capable of rehydrating overnight than broadleaves across gradients of soil and atmospheric drought, suggesting less resilience toward transient stress. In particular, Norway spruce and Scots pine experienced extensive stem dehydration. Our high-resolution dendrometer network was suitable to disentangle the effects of a severe heatwave on tree growth and desiccation at large-spatial scales in situ, and provided insights on which species may be more vulnerable to climate extremes.

Physiological responses of Norway spruce (Picea abies) seedlings to drought stress.

Four-year-old seedlings of Picea abies [L.] Karst (Norway spruce) were grown in semi-controlled conditions with three watering regimes. The seedlings in the control group (c) were watered to prevent any dehydration effect. The two remaining groups were subjected to mild (ms) and severe water stress (ss), respectively. The following physiological variables were monitored until ss seedlings began to die: leaf water potential (psi(L)), stomatal conductance (g(s)), CO(2) exchange (P(N)), free proline content (Pro), total chlorophyll (a + b) concentration (Chl(t)) and the maximal photochemical efficiency of photosystem II (F(v)/F(m)). The results indicate that not all observed physiological parameters display the same degree of sensitivity to dehydration. After Day 12 of dehydration, psi(L) of ss seedlings was already significantly lower than that of the two other groups. On Day 26, significant differences in psi(L) were recorded among all treatments. Decreasing values of water potential were accompanied by early changes in P(N), g(s) and Pro. A significant decrease in Chl(t) and F(v)/F(m) were only observed during the more advanced stages of dehydration. These results demonstrate that the drought response of P. abies seedlings include a number of parallel physiological and biochemical changes in concert, enhancing the capability of plants to survive and grow during drought periods, but only to a point.

Variation in Ecophysiological Traits and Drought Tolerance of Beech (Fagus sylvatica L.) Seedlings from Different Populations.

Frequency and intensity of heat waves and drought events are expected to increase in Europe due to climate change. European beech (Fagus sylvatica L.) is one of the most important native tree species in Europe. Beech populations originating throughout its native range were selected for common-garden experiments with the aim to determine whether there are functional variations in drought stress responses among different populations. One-year old seedlings from four to seven beech populations were grown and drought-treated in a greenhouse, replicating the experiment at two contrasting sites, in Italy (Mediterranean mountains) and Germany (Central Europe). Experimental findings indicated that: (1) drought (water stress) mainly affected gas exchange describing a critical threshold of drought response between 30 and 26% SWA for photosynthetic rate and Ci/Ca, respectively; (2) the Ci to Ca ratio increased substantially with severe water stress suggesting a stable instantaneous water use efficiency and an efficient regulation capacity of water balance achieved by a tight stomatal control; (3) there was a different response to water stress among the considered beech populations, differently combining traits, although there was not a well-defined variability in drought tolerance. A combined analysis of functional and structural traits for detecting stress signals in beech seedlings is suggested to assess plant performance under limiting moisture conditions and, consequently, to estimate evolutionary potential of beech under a changing environmental scenario.

Desiccation and Mortality Dynamics in Seedlings of Different European Beech (Fagus sylvatica L.) Populations under Extreme Drought Conditions.

European beech (Fagus sylvatica L., hereafter beech), one of the major native tree species in Europe, is known to be drought sensitive. Thus, the identification of critical thresholds of drought impact intensity and duration are of high interest for assessing the adaptive potential of European beech to climate change in its native range. In a common garden experiment with one-year-old seedlings originating from central and marginal origins in six European countries (Denmark, Germany, France, Romania, Bosnia-Herzegovina, and Spain), we applied extreme drought stress and observed desiccation and mortality processes among the different populations and related them to plant water status (predawn water potential, ΨPD) and soil hydraulic traits. For the lethal drought assessment, we used a critical threshold of soil water availability that is reached when 50% mortality in seedling populations occurs (LD50SWA). We found significant population differences in LD50SWA (10.5-17.8%), and mortality dynamics that suggest a genetic difference in drought resistance between populations. The LD50SWA values correlate significantly with the mean growing season precipitation at population origins, but not with the geographic margins of beech range. Thus, beech range marginality may be more due to climatic conditions than to geographic range. The outcome of this study suggests the genetic variation has a major influence on the varying adaptive potential of the investigated populations.