Rickettsia parkeri in Amblyomma americanum ticks, Tennessee and Georgia, USA.

To determine the geographic distribution of the newly recognized human pathogen Rickettsia parkeri, we looked for this organism in ticks from Tennessee and Georgia, USA. Using PCR and sequence analysis, we identified R. parkeri in 2 Amblyomma americanum ticks. This rickettsiosis may be underdiagnosed in the eastern United States.

Giardia in beaver (Castor canadensis) and nutria (Myocastor coypus) from east Texas.

We examined the prevalence of Giardia sp. infection in nuisance beaver (Castor canadensis) and nutria (Myocastor coypus) in east Texas. From October 1992 through September 1993, 100 beaver and 30 nutria were collected in routine wildlife management activities conducted by the U.S. Department of Agriculture-Animal and Plant Health Inspection Service-Texas Animal Damage Control Service. Fecal and duodenal mucoid samples were preserved from each animal. Fecal samples were examined for the occurrence of Giardia sp. cysts using the Merifluor immunoassay detection kit: 30 beaver (30.0%) and 20 nutria (66.7%) were positive for Giardia sp. Duodenal mucoid samples were examined for Giardia sp. trophozoites using trichrome staining. with 26 beaver (26.0%) and 20 nutria (73.3%) testing positive. Combining both techniques resulted in 33 beaver (33.0%) and 22 nutria (73.3%) testing positive for Giardia sp. We found no relationship between Giardia sp. and host age, sex, river system, habitat, county, or season in beaver. However, a relationship was found when season and habitat were considered together. No relationship was found between Giardia sp. and age, river system, habitat, county, or season in nutria: however, more males (87.5%) were infected than females (46.4%).

Prevalence of Ehrlichia chaffeensis and Ehrlichia ewingii in ticks from Tennessee.

Human ehrlichiosis is the second most common tick-borne disease reported in Tennessee after Rocky Mountain spotted fever. Two closely related ehrlichiae, Ehrlichia chaffeensis and Ehrlichia ewingii, are both causative agents of human disease and are transmitted by Amblyomma americanum, the lone star tick. Prevalence rates and distribution patterns of these pathogens among ticks in Tennessee are currently unknown. To understand prevalence and exposure risk of Ehrlichia spp., we tested 616 ticks (309 Amblyomma americanum (L.), 277 Dermacentor variabilis (Say), 17 Ixodes texanus (Banks), 7 Ixodes cookei Packard, 4 Ixodes scapularis (Say), and 2 Amblyomma maculatum Koch) from 46 counties for E. chaffeensis and 324 ticks (238 A. americanum and 86 D. variabilis) from 29 counties for E. ewingii. Overall, E. chaffeensis was detected in 2.6% (8/309) of A. americanum and E. ewingii in 0.8% (2/238). Ehrlichia spp. DNA was not detected in any tick species other than A. americanum. Although sample sizes were low in many counties, all positive ticks were identified in the Interior Plateau and Southeastern Plains ecoregions which is where the majority of human ehrlichiosis cases are reported from Tennessee (e.g., 66.3% of the human cases in 2008 are from the Interior Plateau ecoregion). The data from this pathogen survey combined with frequent human case reports from certain areas indicate potential "hot spots" for ehrlichiosis infection. Targeted vector control interventions in these areas may help decrease human ehrlichiosis transmission.

Absence of Rickettsia rickettsii and occurrence of other spotted fever group rickettsiae in ticks from Tennessee.

Rocky Mountain spotted fever (RMSF) is the most common tick-borne illness in Tennessee. Little is known about the occurrence of R. rickettsii, the causative agent, in ticks in Tennessee. To better understand the prevalence and distribution of rickettsial agents in ticks, we tested 1,265 Amblyomma, Dermacentor, and Ixodes adult and nymphal ticks. Additionally, we tested 231 Amblyomma americanum larvae. Ticks were collected from 49 counties from humans, wild animals, domestic canines, and flannel drags. Spotted fever group rickettsiae (SFGR) DNA was detected by polymerase chain reaction (PCR) in 32% of adult and nymphal ticks. A total minimum infection rate of 85.63 was found in larval pools tested. Three rickettsial species, Rickettsia montana, Rickettsia amblyommii, and Rickettsia cooleyi were identified by molecular analysis. Rickettsia rickettsii was not detected. This study suggests that some RMSF cases reported in Tennessee may be caused by cross-reactivity with other SFGR antigenically related to R. rickettsii.