RESUMO
In the face, symmetry is established when bilateral streams of neural crest cells leave the neural tube at the same time, follow identical migration routes and then give rise to the facial prominences. However, developmental instability exists, particularly surrounding the steps of lip fusion. The causes of instability are unknown but inability to cope with developmental fluctuations are a likely cause of congenital malformations, such as non-syndromic orofacial clefts. Here, we tracked cell movements over time in the frontonasal mass, which forms the facial midline and participates in lip fusion, using live-cell imaging of chick embryos. Our mathematical examination of cell velocity vectors uncovered temporal fluctuations in several parameters, including order/disorder, symmetry/asymmetry and divergence/convergence. We found that treatment with a Rho GTPase inhibitor completely disrupted the temporal fluctuations in all measures and blocked morphogenesis. Thus, we discovered that genetic control of symmetry extends to mesenchymal cell movements and that these movements are of the type that could be perturbed in asymmetrical malformations, such as non-syndromic cleft lip. This article has an associated 'The people behind the papers' interview.
Assuntos
Movimento Celular , Face/fisiologia , Mesoderma/crescimento & desenvolvimento , Crista Neural/fisiologia , Actomiosina , Animais , Encéfalo/anatomia & histologia , Encéfalo/crescimento & desenvolvimento , Divisão Celular , Proliferação de Células , Embrião de Galinha , Galinhas , Fenda Labial/genética , Fissura Palatina/genética , Olho/anatomia & histologia , Olho/crescimento & desenvolvimento , Face/anormalidades , Regulação da Expressão Gênica no Desenvolvimento , Mesoderma/anatomia & histologia , Morfogênese/genética , Crista Neural/anatomia & histologiaRESUMO
Human pluripotent stem cells (hPSCs) hold promise for regenerative medicine to replace essential cells that die or become dysfunctional. In some cases, these cells can be used to form clusters whose size distribution affects the growth dynamics. We develop models to predict cluster size distributions of hPSCs based on several plausible hypotheses, including (0) exponential growth, (1) surface growth, (2) Logistic growth, and (3) Gompertz growth. We use experimental data to investigate these models. A partial differential equation for the dynamics of the cluster size distribution is used to fit parameters (rates of growth, mortality, etc.). A comparison of the models using their mean squared error and the Akaike Information criterion suggests that Models 1 (surface growth) or 2 (Logistic growth) best describe the data.
Assuntos
Conceitos Matemáticos , Modelos Biológicos , Células-Tronco Pluripotentes , Humanos , Células-Tronco Pluripotentes/citologia , Proliferação de Células , Técnicas de Cultura de Células , Células CultivadasRESUMO
Capsular contracture is a painful deformation of scar-tissue that may form around an implant in post-breast cancer reconstruction or cosmetic surgery. Inflammation due to surgical trauma or contamination in the tissue around the implant could account for recruitment of immune cells, and transdifferentiation of resident fibroblasts into cells that deposit abnormally thick collagen. Here we examine this hypothesis using a mathematical model for interacting macrophages, fibroblasts, myofibroblasts, and collagen. Our model demonstrates that cellular response can, together with inflammatory cell recruitment, account for prognoses.
Assuntos
Implantes de Mama , Neoplasias da Mama , Contratura , Humanos , Feminino , Neoplasias da Mama/cirurgia , Implantes de Mama/efeitos adversos , Conceitos Matemáticos , Modelos Biológicos , ColágenoRESUMO
In epithelial-mesenchymal transition (EMT), cells organized into sheets break away and become motile mesenchymal cells. EMT plays a crucial role in wound healing, embryonic development, and cancer metastasis. Intracellular signaling in response to mechanical, topographic, or chemical stimuli can promote EMT. We present a multiscale model for EMT downstream of the protein YAP, which suppresses the cell-cell adhesion protein E-cadherin and activates the GTPase Rac1 that enhances cell migration. We first propose an ordinary differential equation (ODE) model for intracellular YAP/Rac1/E-cadherin interactions. The ODE model dynamics are bistable, accounting for both motile loose cells and adherent slower cells. We incorporate this model into a cellular Potts model simulation of two-dimensional wound healing using the open-source platform Morpheus. We show that, under suitable stimuli representing topographic cues, the sheet exhibits finger-like projections and EMT. Morphological differences and quantitative differences in YAP levels as well as variations in cell speed across the sheet are consistent with previous experimental observations of epithelial sheets grown on topographic features in vitro. The simulation is also consistent with experiments that knock down or overexpress YAP, inhibit Rac1, or block E-cadherin.
Assuntos
Transição Epitelial-Mesenquimal , Transdução de Sinais , Caderinas/metabolismo , Linhagem Celular Tumoral , Movimento CelularRESUMO
The intrinsic polarity of migrating cells is regulated by spatial distributions of protein activity. Those proteins (Rho-family GTPases, such as Rac and Rho) redistribute in response to stimuli, determining the cell front and back. Reaction-diffusion equations with mass conservation and positive feedback have been used to explain initial polarization of a cell. However, the sensitivity of a polar cell to a reversal stimulus has not yet been fully understood. We carry out a PDE bifurcation analysis of two polarity models to investigate routes to repolarization: (1) a single-GTPase ("wave-pinning") model and (2) a mutually antagonistic Rac-Rho model. We find distinct routes to reversal in (1) vs. (2). We show numerical simulations of full PDE solutions for the RD equations, demonstrating agreement with predictions of the bifurcation results. Finally, we show that simulations of the polarity models in deforming 1D model cells are consistent with biological experiments.
Assuntos
Polaridade Celular , Corpos Polares , Polaridade Celular/fisiologia , Conceitos Matemáticos , Modelos Biológicos , Corpos Polares/metabolismo , Proteínas rho de Ligação ao GTP/metabolismoRESUMO
The small GTPases Rac and Rho are known to regulate eukaryotic cell shape, promoting front protrusion (Rac) or rear retraction (Rho) of the cell edge. Such cell deformation changes the contact and adhesion of cell to the extracellular matrix (ECM), while ECM signaling through integrin receptors also affects GTPase activity. We develop and investigate a model for this three-way feedback loop in 1D and 2D spatial domains, as well as in a fully deforming 2D cell shapes with detailed adhesion-bond biophysics. The model consists of reaction-diffusion equations solved numerically with open-source software, Morpheus, and with custom-built cellular Potts model simulations. We find a variety of patterns and cell behaviors, including persistent polarity, flipped front-back cell polarity oscillations, spiral waves, and random protrusion-retraction. We show that the observed spatial patterns depend on the cell shape, and vice versa.
Assuntos
Matriz Extracelular , Transdução de Sinais , Adesão Celular , Movimento Celular , Polaridade Celular , Forma Celular , Matriz Extracelular/metabolismo , Proteínas rho de Ligação ao GTP/metabolismoRESUMO
Mathematical and computational models can assist in gaining an understanding of cell behavior at many levels of organization. Here, we review models in the literature that focus on eukaryotic cell motility at 3 size scales: intracellular signaling that regulates cell shape and movement, single cell motility, and collective cell behavior from a few cells to tissues. We survey recent literature to summarize distinct computational methods (phase-field, polygonal, Cellular Potts, and spherical cells). We discuss models that bridge between levels of organization, and describe levels of detail, both biochemical and geometric, included in the models. We also highlight links between models and experiments. We find that models that span the 3 levels are still in the minority.
Assuntos
Movimento Celular/fisiologia , Simulação por Computador , Modelos Biológicos , Forma Celular , Citoesqueleto/metabolismo , Transdução de SinaisRESUMO
The polarization and motility of eukaryotic cells depends on assembly and contraction of the actin cytoskeleton and its regulation by proteins called GTPases. The activity of GTPases causes assembly of filamentous actin (by GTPases Cdc42, Rac), resulting in protrusion of the cell edge. Mathematical models for GTPase dynamics address the spontaneous formation of patterns and nonuniform spatial distributions of such proteins in the cell. Here we revisit the wave-pinning model for GTPase-induced cell polarization, together with a number of extensions proposed in the literature. These include introduction of sources and sinks of active and inactive GTPase (by the group of A. Champneys), and negative feedback from F-actin to GTPase activity. We discuss these extensions singly and in combination, in 1D, and 2D static domains. We then show how the patterns that form (spots, waves, and spirals) interact with cell boundaries to create a variety of interesting and dynamic cell shapes and motion.
Assuntos
Movimento Celular , Células Eucarióticas , GTP Fosfo-Hidrolases , Citoesqueleto de Actina/metabolismo , Movimento Celular/fisiologia , Forma Celular , Células Eucarióticas/citologia , Células Eucarióticas/enzimologia , GTP Fosfo-Hidrolases/metabolismoRESUMO
Recent synthetic biology experiments reveal that signaling modules designed to target cell-cell adhesion enable self-organization of multicellular structures Toda et al (2018 Science 361 156-162). Changes in homotypic adhesion that arise through contact-dependent signaling networks result in sorting of an aggregate into two- or three-layered structures. Here we investigate the formation, maintenance, and robustness of such self-organization in the context of a computational model. To do so, we use an established model for Notch/ligand signaling within cells to set up differential E-cadherin expression. This signaling model is integrated with the cellular Potts model to track state changes, adhesion, and cell sorting in a group of cells. The resulting multicellular structures are in accordance with those observed in the experimental reference. In addition to reproducing these experimental results, we track the dynamics of the evolving structures and cell states to understand how such morphologies are dynamically maintained. This appears to be an important developmental principle that was not emphasized in previous models. Our computational model facilitates more detailed understanding of the link between intra- and intercellular signaling, spatio-temporal rearrangement, and emergent behavior at the scale of hundred(s) of cells.
Assuntos
Adesão Celular , Transdução de Sinais , Movimento Celular , Biologia Computacional , Modelos Biológicos , Biologia SintéticaRESUMO
Single and collective cell dynamics, cell shape changes, and cell migration can be conveniently represented by the Cellular Potts Model, a computational platform based on minimization of a Hamiltonian. Using the fact that a force field is easily derived from a scalar energy (F = -∇H), we develop a simple algorithm to associate effective forces with cell shapes in the CPM. We predict the traction forces exerted by single cells of various shapes and sizes on a 2D substrate. While CPM forces are specified directly from the Hamiltonian on the cell perimeter, we approximate the force field inside the cell domain using interpolation, and refine the results with smoothing. Predicted forces compare favorably with experimentally measured cellular traction forces. We show that a CPM model with internal signaling (such as Rho-GTPase-related contractility) can be associated with retraction-protrusion forces that accompany cell shape changes and migration. We adapt the computations to multicellular systems, showing, for example, the forces that a pair of swirling cells exert on one another, demonstrating that our algorithm works equally well for interacting cells. Finally, we show forces exerted by cells on one another in classic cell-sorting experiments.
Assuntos
Forma Celular/fisiologia , Modelos Biológicos , Algoritmos , Fenômenos Biofísicos , Adesão Celular/fisiologia , Agregação Celular/fisiologia , Comunicação Celular/fisiologia , Movimento Celular/fisiologia , Biologia Computacional , Simulação por Computador , Humanos , Transdução de Sinais/fisiologiaRESUMO
Cell polarization requires redistribution of specific proteins to the nascent front and back of a eukaryotic cell. Among these proteins are Rac and Rho, members of the small GTPase family that regulate the actin cytoskeleton. Rac promotes actin assembly and protrusion of the front edge, whereas Rho activates myosin-driven contraction at the back. Mathematical models of cell polarization at many levels of detail have appeared. One of the simplest based on "wave-pinning" consists of a pair of reaction-diffusion equations for a single GTPase. Mathematical analysis of wave-pinning so far is largely restricted to static domains in one spatial dimension. Here, we extend the analysis to cells that change in size, showing that both shrinking and growing cells can lose polarity. We further consider the feedback between mechanical tension, GTPase activation, and cell deformation in both static, growing, shrinking, and moving cells. Special cases (spatially uniform cell chemistry, the absence or presence of mechanical feedback) are analyzed, and the full model is explored by simulations in 1D. We find a variety of novel behaviors, including "dilution-induced" oscillations of Rac activity and cell size, as well as gain or loss of polarization and motility in the model cell.
Assuntos
Tamanho Celular , GTP Fosfo-Hidrolases/metabolismo , Modelos Biológicos , Citoesqueleto de Actina/metabolismo , Animais , Fenômenos Biomecânicos , Movimento Celular/fisiologia , Polaridade Celular/fisiologia , Simulação por Computador , Humanos , Conceitos Matemáticos , Transdução de Sinais , Análise de Célula Única , Proteínas rac de Ligação ao GTP/metabolismo , Proteínas rho de Ligação ao GTP/metabolismoRESUMO
Cell polarization and directional cell migration can display random, persistent, and oscillatory dynamic patterns. However, it is not clear whether these polarity patterns can be explained by the same underlying regulatory mechanism. Here, we show that random, persistent, and oscillatory migration accompanied by polarization can simultaneously occur in populations of melanoma cells derived from tumors with different degrees of aggressiveness. We demonstrate that all of these patterns and the probabilities of their occurrence are quantitatively accounted for by a simple mechanism involving a spatially distributed, mechanochemical feedback coupling the dynamically changing extracellular matrix (ECM)-cell contacts to the activation of signaling downstream of the Rho-family small GTPases. This mechanism is supported by a predictive mathematical model and extensive experimental validation, and can explain previously reported results for diverse cell types. In melanoma, this mechanism also accounts for the effects of genetic and environmental perturbations, including mutations linked to invasive cell spread. The resulting mechanistic understanding of cell polarity quantitatively captures the relationship between population variability and phenotypic plasticity, with the potential to account for a wide variety of cell migration states in diverse pathological and physiological conditions.
Assuntos
Polaridade Celular/fisiologia , Retroalimentação Fisiológica , Melanoma/metabolismo , Neoplasias Cutâneas/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Forma Celular , Progressão da Doença , Matriz Extracelular/metabolismo , Regulação Neoplásica da Expressão Gênica , Humanos , Melanoma/patologia , Modelos Teóricos , Mutação , Invasividade Neoplásica , Oscilometria , Fenótipo , Transdução de Sinais , Neoplasias Cutâneas/patologia , Proteínas rho de Ligação ao GTP/metabolismoRESUMO
We propose a model to explain the spontaneous collective migration of neural crest cells in the absence of an external gradient of chemoattractants. The model is based on the dynamical interaction between Rac1 and RhoA that is known to regulate the polarization, contact inhibition and co-attraction of neural crest cells. Coupling the reaction-diffusion equations for active and inactive Rac1 and RhoA on the cell membrane with a mechanical model for the overdamped motion of membrane vertices, we show that co-attraction and contact inhibition cooperate to produce persistence of polarity in a cluster of neural crest cells by suppressing the random onset of Rac1 hotspots that may mature into new protrusion fronts. This produces persistent directional migration of cell clusters in corridors. Our model confirms a prior hypothesis that co-attraction and contact inhibition are key to spontaneous collective migration, and provides an explanation of their cooperative working mechanism in terms of Rho GTPase signaling. The model shows that the spontaneous migration is more robust for larger clusters, and is most efficient in a corridor of optimal confinement.
Assuntos
Movimento Celular/fisiologia , Polaridade Celular/fisiologia , Crista Neural/metabolismo , Comunicação Celular/fisiologia , Membrana Celular/metabolismo , Simulação por Computador , Inibição de Contato/fisiologia , Crista Neural/fisiologia , Transdução de Sinais , Proteínas rac1 de Ligação ao GTP/fisiologia , Proteína rhoA de Ligação ao GTP/metabolismoRESUMO
Correlated random walks (CRW) have been explored in many settings, most notably in the motion of individuals in a swarm or flock. But some subcellular systems such as growth or disassembly of bio-polymers can also be described with similar models and understood using related mathematical methods. Here we consider two examples of growing cytoskeletal elements, actin and microtubules. We use CRW or generalized CRW-like PDEs to model their spatial distributions. In each case, the linear models can be reduced to a Telegrapher's equation. A combination of explicit solutions (in one case) and numerical solutions (in the other) demonstrates that the approach to steady state can be accompanied by (decaying) waves.
Assuntos
Actinas/metabolismo , Microtúbulos/metabolismo , Modelos Biológicos , Citoesqueleto de Actina/metabolismo , Animais , Humanos , Modelos Lineares , Conceitos Matemáticos , Movimento/fisiologia , Análise Espaço-TemporalRESUMO
Regulators of the actin cytoskeleton such Rho GTPases can modulate forces developed in cells by promoting actomyosin contraction. At the same time, through mechanosensing, tension is known to affect the activity of Rho GTPases. What happens when these effects act in concert? Using a minimal model (1 GTPase coupled to a Kelvin-Voigt element), we show that two-way feedback between signaling ('RhoA') and mechanical tension (stretching) leads to a spectrum of cell behaviors, including contracted or relaxed cells, and cells that oscillate between these extremes. When such 'model cells' are connected to one another in a row or in a 2D sheet ('epithelium'), we observe waves of contraction/relaxation and GTPase activity sweeping through the tissue. The minimal model lends itself to full bifurcation analysis, and suggests a mechanism that explains behavior observed in the context of development and collective cell behavior.
Assuntos
Actomiosina/metabolismo , Células Epiteliais/metabolismo , Proteínas rho de Ligação ao GTP/metabolismo , Citoesqueleto de Actina/metabolismo , Animais , Modelos Biológicos , Transdução de Sinais , Estresse MecânicoRESUMO
Protrusion and retraction of lamellipodia are common features of eukaryotic cell motility. As a cell migrates through its extracellular matrix (ECM), lamellipod growth increases cell-ECM contact area and enhances engagement of integrin receptors, locally amplifying ECM input to internal signaling cascades. In contrast, contraction of lamellipodia results in reduced integrin engagement that dampens the level of ECM-induced signaling. These changes in cell shape are both influenced by, and feed back onto ECM signaling. Motivated by experimental observations on melanoma cells lines (1205Lu and SBcl2) migrating on fibronectin (FN) coated topographic substrates (anisotropic post-density arrays), we probe this interplay between intracellular and ECM signaling. Experimentally, cells exhibited one of three lamellipodial dynamics: persistently polarized, random, or oscillatory, with competing lamellipodia oscillating out of phase (Park et al., 2017). Pharmacological treatments, changes in FN density, and substrate topography all affected the fraction of cells exhibiting these behaviours. We use these observations as constraints to test a sequence of hypotheses for how intracellular (GTPase) and ECM signaling jointly regulate lamellipodial dynamics. The models encoding these hypotheses are predicated on mutually antagonistic Rac-Rho signaling, Rac-mediated protrusion (via activation of Arp2/3 actin nucleation) and Rho-mediated contraction (via ROCK phosphorylation of myosin light chain), which are coupled to ECM signaling that is modulated by protrusion/contraction. By testing each model against experimental observations, we identify how the signaling layers interact to generate the diverse range of cell behaviors, and how various molecular perturbations and changes in ECM signaling modulate the fraction of cells exhibiting each. We identify several factors that play distinct but critical roles in generating the observed dynamic: (1) competition between lamellipodia for shared pools of Rac and Rho, (2) activation of RhoA by ECM signaling, and (3) feedback from lamellipodial growth or contraction to cell-ECM contact area and therefore to the ECM signaling level.
Assuntos
Fenômenos Fisiológicos Celulares/fisiologia , Modelos Biológicos , Biologia Computacional , Matriz Extracelular/fisiologia , Proteínas rho de Ligação ao GTP/metabolismoRESUMO
Collective cell migration plays an important role in development. Here, we study the posterior lateral line primordium (PLLP) a group of about 100 cells, destined to form sensory structures, that migrates from head to tail in the zebrafish embryo. We model mutually inhibitory FGF-Wnt signalling network in the PLLP and link tissue subdivision (Wnt receptor and FGF receptor activity domains) to receptor-ligand parameters. We then use a 3D cell-based simulation with realistic cell-cell adhesion, interaction forces, and chemotaxis. Our model is able to reproduce experimentally observed motility with leading cells migrating up a gradient of CXCL12a, and trailing (FGF receptor active) cells moving actively by chemotaxis towards FGF ligand secreted by the leading cells. The 3D simulation framework, combined with experiments, allows an investigation of the role of cell division, chemotaxis, adhesion, and other parameters on the shape and speed of the PLLP. The 3D model demonstrates reasonable behaviour of control as well as mutant phenotypes.
Assuntos
Padronização Corporal , Movimento Celular , Polaridade Celular , Peixe-Zebra/embriologia , Animais , Biologia Computacional , Modelos BiológicosRESUMO
We apologize for the error in the references.
RESUMO
Molecular motors such as kinesin and dynein are responsible for transporting material along microtubule networks in cells. In many contexts, motor dynamics can be modelled by a system of reaction-advection-diffusion partial differential equations (PDEs). Recently, quasi-steady-state (QSS) methods have been applied to models with linear reactions to approximate the behaviour of the full PDE system. Here, we extend this QSS reduction methodology to certain nonlinear reaction models. The QSS method relies on the assumption that the nonlinear binding and unbinding interactions of the cellular motors occur on a faster timescale than the spatial diffusion and advection processes. The full system dynamics are shown to be well approximated by the dynamics on the slow manifold. The slow manifold is parametrized by a single scalar quantity that satisfies a scalar nonlinear PDE, called the QSS PDE. We apply the QSS method to several specific nonlinear models for the binding and unbinding of molecular motors, and we use the resulting approximations to draw conclusions regarding the parameter dependence of the spatial distribution of motors for these models.