RESUMO
An excessive cholesterol level can lead to cardiovascular diseases, such as stroke, hypertension, and myocardial infarction. A non-invasive, painless method of determining the cholesterol level in blood would improve the user's convenience. To provide rapid and accurate determination of cholesterol, we have developed a simple, disposable, enzyme-based electrochemical biosensor that can detect salivary cholesterol. It is possible to detect low concentrations of cholesterol in saliva using the optimized vertical structure of the platinum nano-cluster (Pt-NC) and the immobilization of a proper volume of an enzyme. The biosensor exhibited a linear range from 2 to 486 µM, the limit of detection was about 2 µM, and the sensitivity of the sensor was calculated to be 132 µA mM-1 cm-2. It also showed good specificity for ascorbic acid, uric acid, dopamine, glucose, and lactate. In a test with an actual sample, the performance of the biosensor was confirmed by measuring total cholesterol in the saliva of a patient with hyperlipidemia. The cholesterol levels measured in the saliva of three patients with hyperlipidemia were 520, 460, and 290 µM. Therefore, the Pt-NC based enzyme sensor is a promising candidate for the detection of cholesterol in human saliva.
Assuntos
Técnicas Biossensoriais/métodos , Colesterol/análise , Nanoestruturas/química , Platina/química , Saliva/química , Ácido Ascórbico/química , Colesterol Oxidase/química , Colesterol Oxidase/metabolismo , Técnicas Eletroquímicas , Enzimas Imobilizadas/química , Enzimas Imobilizadas/metabolismo , Glucose/química , Humanos , Hiperlipidemias/diagnóstico , Limite de Detecção , Ácido Úrico/químicaRESUMO
In this work, a Lab-on-a-Chip (LOC) platform is used to electromagnetically actuate magnetic bead chains for an enhanced immunoassay. Custom-made electromagnets generate a magnetic field to form, rotate, lift and lower the magnetic bead chains (MBCs). The cost-effective, disposable LOC platform was made with a polymer substrate and an on-chip electrochemical sensor patterned via the screen-printing process. The movement of the MBCs is controlled to improve the electrochemical signal up to 230% when detecting beta-type human chorionic gonadotropin (ß-hCG). Thus, the proposed on-chip MBC-based immunoassay is applicable for rapid, qualitative electrochemical point-of-care (POC) analysis.
Assuntos
Gonadotropina Coriônica Humana Subunidade beta/análise , Imunoensaio/instrumentação , Dispositivos Lab-On-A-Chip , Técnicas Biossensoriais/economia , Técnicas Biossensoriais/instrumentação , Desenho de Equipamento , Humanos , Imunoensaio/economia , Limite de Detecção , Campos Magnéticos , Imãs/química , Técnicas Analíticas Microfluídicas/economia , Técnicas Analíticas Microfluídicas/instrumentaçãoRESUMO
Preterm labor occurs in about 12% of all pregnancies, accounting for at least 75% of neonatal deaths. The neonatal outcomes depend on mainly the gestational age at delivery. The lower the gestational age, the higher the risk of morbidity and mortality. The management of preterm labor involves early detection of high-risk women, prevention and treatment. In this study, the feasibility and stability of implantable cuff electrodes detection and inhibition of uterine contractions for preterm labor treatment were investigated by in-vivo mouse test. In order to check functionality of implanted electrodes, acute in-vivo test at mouse uterosacral ligament was performed by using cuff electrode which stimulate and record nerve activity. As results, the electrical stimulation via a stimulating cuff electrode at mouse uterosacral ligament was properly applied and nerve fiber's signal was recorded via the recording electrode. In addition, the implanted cuff electrodes could obtain stable recording signal to the periodic electrical stimulation for 7 weeks.