In vivo characterization of electroactive biofilms inside porous electrodes with MR Imaging.

Identifying the limiting processes of electroactive biofilms is key to improve the performance of bioelectrochemical systems (BES). For modelling and developing BES, spatial information of transport phenomena and biofilm distribution are required and can be determined by Magnetic Resonance Imaging (MRI) in vivo, in situ and in operando even inside opaque porous electrodes. A custom bioelectrochemical cell was designed that allows MRI measurements with a spatial resolution of 50 µm inside a 500 µm thick porous carbon electrode. The MRI data showed that only a fraction of the electrode pore space is colonized by the Shewanella oneidensis MR-1 biofilm. The maximum biofilm density was observed inside the porous electrode close to the electrode-medium interface. Inside the biofilm, mass transport by diffusion is lowered down to 45% compared to the bulk growth medium. The presented data and the methods can be used for detailed models of bioelectrochemical systems and for the design of improved electrode structures.

Local Acidification Limits the Current Production and Biofilm Formation of Shewanella oneidensis MR-1 With Electrospun Anodes.

The anodic current production of Shewanella oneidensis MR-1 is typically lower compared to other electroactive bacteria. The main reason for the low current densities is the poor biofilm growth on most anode materials. We demonstrate that the high current production of Shewanella oneidensis MR-1 with electrospun anodes exhibits a similar threshold current density as dense Geobacter spp biofilms. The threshold current density is a result of local acidification in the biofilm. Increasing buffer concentration from 10 to 40 mM results in a 1.8-fold increase of the current density [(590 ± 25) µA cm-2] while biofilm growth stimulation by riboflavin has little effect on the current production. The current production of a reference material below the threshold did not respond to the increased buffer concentration but could be enhanced by supplemented riboflavin that stimulated the biofilm growth. Our results suggest that the current production with S. oneidensis is limited (1) by the biofilm growth on the anode that can be enhanced by the choice of the electrode material, and (2) by the proton transport through the biofilm and the associated local acidification.

Characterization of microbial current production as a function of microbe-electrode-interaction.

Microbe-electrode-interactions are keys for microbial fuel cell technology. Nevertheless, standard measurement routines to analyze the interplay of microbial physiology and material characteristics have not been introduced yet. In this study, graphite anodes with varying surface properties were evaluated using pure cultures of Shewanella oneidensis and Geobacter sulfurreducens, as well as defined and undefined mixed cultures. The evaluation routine consisted of a galvanostatic period, a current sweep and an evaluation of population density. The results show that surface area correlates only to a certain extent with population density and anode performance. Furthermore, the study highlights a strain-specific microbe-electrode-interaction, which is affected by the introduction of another microorganism. Moreover, evidence is provided for the possibility of translating results from pure culture to undefined mixed species experiments. This is the first study on microbe-electrode-interaction that systematically integrates and compares electrochemical and biological data.

Systematic screening of carbon-based anode materials for microbial fuel cells with Shewanella oneidensis MR-1.

We present a systematic screening of carbon-based anode materials for microbial fuel cells with Shewanella oneidensis MR-1. Under anoxic conditions nanoporous activated carbon cloth is a superior anode material in terms of current density normalized to the projected anode area and anode volume (24.0±0.3 µA cm(-2) and 482±7 µA cm(-3) at -0.2 vs. SCE, respectively). The good performance can be attributed to the high specific surface area of the material, which is available for mediated electron transfer through self-secreted flavins. Under aerated conditions no influence of the specific surface area is observed, which we attribute to a shift from primary indirect electron transfer by mediators to direct electron transfer via adherent cells. Furthermore, we show that an aerated initial growth phase enhances the current density under subsequent anoxic conditions fivefold when compared to a similar experiment that was conducted under permanently anoxic conditions.