RESUMO
The DNA sequence (AT)26 of barley genome has been cloned. This sequence is arranged in intraspecific locus and is repeated 1500 times per haploid genome. This fragment is not translated and can form cruciform structures in the AT region.
Assuntos
DNA/genética , Hordeum/genética , Sequências Repetitivas de Ácido Nucleico , Sequência de Bases , Clonagem Molecular , DNA/metabolismo , DNA Super-Helicoidal/metabolismo , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Endodesoxirribonucleases/metabolismo , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Polimorfismo de Fragmento de Restrição , Endonucleases Específicas para DNA e RNA de Cadeia Simples/metabolismoRESUMO
After the accident at the Chernobyl nuclear power plant, a considerable increase in the incidence of thyroid cancer among children in Belarus was observed. In the present study, the frequency of the c-ret protooncogene rearrangements in samples of thyroid carcinomas resected and diagnosed in 1998 from individuals in Belarus was investigated. The ret/PTC1 oncogene was detected in 19% of the samples, and the ret/PTC3r1 oncogene, in 14%. The number of ret/PTC1 rearrangements observed in tumor cells from the patients whose age at the time of the accident was from 1 to 10 years, was greater compared to those whose age at the time was from 10 to 20 years, irrespective of the year of surgery (1996 or 1998). The majority of the patients with ret/PTC3r1 rearrangements lived in Gomel oblast, which was contaminated by the Chernobyl meltdown.
Assuntos
Rearranjo Gênico , Neoplasias Induzidas por Radiação/genética , Proteínas de Fusão Oncogênica/genética , Proteínas Oncogênicas/genética , Centrais Elétricas , Liberação Nociva de Radioativos , Neoplasias da Glândula Tireoide/genética , Fatores de Transcrição , Criança , Humanos , Coativadores de Receptor Nuclear , Proteínas Tirosina Quinases , Neoplasias da Glândula Tireoide/etiologia , UcrâniaRESUMO
The peculiarities of alternative CD44 mRNA splicing in thyroid cancer tissue of children from radiocontaminated areas was investigated. CD44 gene expression in thyroid cancer tissues of children exposed to radiation resembled that in spontaneously emerged cancers. It was concluded that CD44 gene expression is not the primary target of radioactive irradiation. Probably, the CD44 mRNA splicing deregulation is the consequence of cancer.