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1.
Planta ; 245(2): 409-424, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27830397

RESUMO

MAIN CONCLUSION: Two subtilisin-like proteases show highly specific and complementary expression patterns in developing grains. These genes label the complete surface of the filial-maternal interface, suggesting a role in filial epithelial differentiation. The cereal endosperm is the most important source of nutrition and raw materials for mankind, as well as the storage compartment enabling initial growth of the germinating plantlets. The development of the different cell types in this tissue is regulated environmentally, genetically and epigenetically, resulting in the formation of top-bottom, adaxial-abaxial and surface-central axes. However, the mechanisms governing the interactions among the different inputs are mostly unknown. We have screened a kernel cDNA library for tissue-specific transcripts as initial step to identify genes relevant in cell differentiation. We report here on the isolation of two maize subtilisin-related genes that show grain-specific, surficial expression. zmsbt1 (Zea mays Subtilisin1) is expressed at the developing aleurone in a time-regulated manner, while zmsbt2 concentrates at the pedicel in front of the endosperm basal transfer layer. We have shown that their presence, early in the maize caryopsis development, is dependent on proper initial tissue determination, and have isolated their promoters to produce transgenic reporter lines that assist in the study of their regulation.


Assuntos
Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Zea mays/crescimento & desenvolvimento , Zea mays/genética , Diferenciação Celular , Endosperma/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Sementes/genética , Sementes/crescimento & desenvolvimento , Serina Proteases/genética , Subtilisinas/genética , Fatores de Tempo
2.
Planta ; 229(2): 235-47, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18830706

RESUMO

Transfer cells have specializations that facilitate the transport of solutes across plant exchange surfaces. ZmMRP-1 is a maize (Zea mays) endosperm transfer cell-specific transcriptional activator that plays a central role in the regulatory pathways controlling transfer cell differentiation and function. The present work investigates the signals controlling the expression of ZmMRP-1 through the production of transgenic lines of maize, Arabidopsis, tobacco and barley containing ZmMRP-1promoter:GUS reporter constructs. The GUS signal predominantly appeared in regions of active transport between source and sink tissues, including nematode-induced feeding structures and at sites of vascular connection between developing organs and the main plant vasculature. In those cases, promoter induction was associated with the initial developmental stages of transport structures. Significantly, transfer cells also differentiated in these regions suggesting that, independent of species, location or morphological features, transfer cells might differentiate in a similar way under the influence of conserved induction signals. In planta and yeast experiments showed that the promoter activity is modulated by carbohydrates, glucose being the most effective inducer.


Assuntos
Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Regiões Promotoras Genéticas/genética , Transativadores/genética , Zea mays/genética , Animais , Arabidopsis/citologia , Arabidopsis/genética , Arabidopsis/parasitologia , Transporte Biológico , Germinação , Hordeum/genética , Nematoides , Especificidade de Órgãos , Plantas Geneticamente Modificadas , Plântula/citologia , Plântula/genética , Sementes/citologia , Sementes/genética , Propriedades de Superfície , Nicotiana/genética , Zea mays/citologia , Zea mays/parasitologia
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