Surface activity and collective behaviour of colloidally stable Janus-like particles at the air-water interface.

In this work we report an experimental study on the surface activity and the collective behaviour of colloidally stable Janus-like silver particles at the air-water interface. The colloidal stability of silver nanoparticles has been enhanced using different capping ligands. Two polymers coated the silver particles: 11-mercaptoundecanoic acid and 1-undecanthiol. These capping ligands adsorbed onto the particle surface are spontaneously rearranged at the air-water interface. This feature leads to Janus behaviour in the silver particles with amphiphilic character. The surface activity of the silver particles at the air-water interface has been measured using pendant drop tensiometry. The Janus-like silver particles revealed a surface activity similar to that shown by conventional amphiphilic molecules but with much larger area per particle. The variation of the surface pressure with the area per particle was described properly using the Frumkin isotherm up to the collapse state. Furthermore, oscillating pendant drop tensiometry provided very useful data on the rheological properties of Janus particle monolayers; these properties depended on the lateral interactions between particles and were closely related to the monolayer microstructure. We revealed the close relationship between the collective behavior and the surface activity of Janus-like silver particles.

Reconfigurable artificial microswimmers with internal feedback.

Self-propelling microparticles are often proposed as synthetic models for biological microswimmers, yet they lack the internally regulated adaptation of their biological counterparts. Conversely, adaptation can be encoded in larger-scale soft-robotic devices but remains elusive to transfer to the colloidal scale. Here, we create responsive microswimmers, powered by electro-hydrodynamic flows, which can adapt their motility via internal reconfiguration. Using sequential capillary assembly, we fabricate deterministic colloidal clusters comprising soft thermo-responsive microgels and light-absorbing particles. Light absorption induces preferential local heating and triggers the volume phase transition of the microgels, leading to an adaptation of the clusters' motility, which is orthogonal to their propulsion scheme. We rationalize this response via the coupling between self-propulsion and variations of particle shape and dielectric properties upon heating. Harnessing such coupling allows for strategies to achieve local dynamical control with simple illumination patterns, revealing exciting opportunities for developing tactic active materials.

Interfacial tension measurements using a new axisymmetric drop/bubble shape technique.

This paper introduces a new mathematical model that is used to compute either the interfacial tension of quiescent axisymmetric pendant/sessile drops and pendant/captive bubbles. This model consists of the Young-Laplace equation, that describes interface shape, together with suitable boundary conditions that guarantee a prescribed volume of drops/bubbles and a fixed position in the capillary. In order to solve the problem numerically, the Young-Laplace equation is discretized by using numerical differentiation and the numerical solutions are obtained applying the well-know Newton method. The paper contains a validation of the new methodology presented for what theoretical bubble/drops are used. Finally, some numerical results are presented for both drops and bubbles of water as well as several surfactant solutions to demonstrate the applicability, versatility and reproducibility of the proposed methodology.

Exploring the colloid-to-polymer transition for ultra-low crosslinked microgels from three to two dimensions.

Microgels are solvent-swollen nano- and microparticles that show prevalent colloidal-like behavior despite their polymeric nature. Here we study ultra-low crosslinked poly(N-isopropylacrylamide) microgels (ULC), which can behave like colloids or flexible polymers depending on dimensionality, compression or other external stimuli. Small-angle neutron scattering shows that the structure of the ULC microgels in bulk aqueous solution is characterized by a density profile that decays smoothly from the center to a fuzzy surface. Their phase behavior and rheological properties are those of soft colloids. However, when these microgels are confined at an oil-water interface, their behavior resembles that of flexible macromolecules. Once monolayers of ultra-low crosslinked microgels are compressed, deposited on solid substrate and studied with atomic-force microscopy, a concentration-dependent topography is observed. Depending on the compression, these microgels can behave as flexible polymers, covering the substrate with a uniform film, or as colloidal microgels leading to a monolayer of particles.

Quantifying mesenchymal stem cells in the mononuclear cell fraction of bone marrow samples obtained for cell therapy.

AIMS: The use of bone marrow mononuclear cells (BMMNCs) as a source of mesenchymal stem cells (MSCs) for therapy has recently attracted the attention of researchers because BMMNCs can be easily obtained and do not require in vitro expansion before their use. This study was designed to quantify the MSC population in bone marrow (BM) samples obtained for cell therapy using flow cytometry to detect the CD271 antigen. MATERIAL AND METHODS: Autologous BM was obtained by posterior superior iliac crest aspiration under topical anesthesia. Mononuclear cells isolated from the BM aspirate on a Ficoll density gradient were used to treat patients with pressure ulcer (n = 13) bone nonunions (n = 3) or diabetic foot ulcers (n = 5). RESULTS: Our flow cytometry data revealed a low percentage as well as a high variability among patients of CD271(+)CD45(-) cells (range, 0.0017 to 0.0201%). All cultured MSC adhered to plastic dishes showing a capacity to differentiate into adipogenic and osteogenic lineages. CONCLUSIONS: Our findings suggested that the success of cell therapy was independent of the number of MSCs present in the BM aspirate used for autologous cell therapy.

First highly regio- and diastereoselective [3+2] cycloaddition of chiral nonracemic Fischer carbene complexes with azomethine ylides: an enantioselective synthesis of (+)-rolipram.

A new procedure for the synthesis of 1,3,4-trisubstituted and 1,4-disubstituted pyrrolidin-2-one derivatives in an enantioselective fashion is reported. The 1,3-dipolar cycloaddition of (+/-)-menthol and (-)-8-phenylmenthol derived Fischer alkoxy alkenyl carbene complexes with in situ generated functionalized azomethine ylides gives the corresponding cycloadducts as chelated tetracarbonyl Fischer carbene complexes. Only one regioisomer is detected in all cases, and the diastereoselectivity of the reaction is very high when (-)-8-phenylmenthol derived carbenes are employed. Oxidation and further transformation of the cycloadducts provide an easy access to pyrrolidin-2-ones. The anti-inflammatory and antidepressant drug (+)-Rolipram is readily prepared in four steps in a 20% overall yield by taking advantage of this newly developed methodology.