Lead effects on Brassica napus photosynthetic organs.

In this study, effects of lead on ultracellular structure and pigment contents of Brassica napus were examined. Pb(II) was added in soluble form to soil prior to sowing. Pb contents were measured in plant organs at the ontogenetic stages of flowering (FL) and physiological maturity (PM). Pigment contents were evaluated through reflectance measurements. Pb content in organs was found to decrease in the order; roots>stems>leaves. Lead content in senescent leaves at FL stage was significantly higher than harvested leaves, strongly suggesting a detoxification mechanism. Leaves and stems harvested at the PM stage showed damage at subcellular level, namely chloroplast disorganization, cell wall damage and presence of osmiophilic bodies. Chlorophyll content increased in the presence of Pb at the FL stage, compared with control; at the PM stage, chlorophyll contents decreased with low Pb concentration but showed no significant differences with control at high Pb soil concentration. The results suggest an increase in antioxidants at low Pb concentration and cell damage at higher lead concentration.

Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus.

The use of biomarkers as a tool to assess responses of organisms exposed to pollutants in toxicity bioassays, as well as in aquatic environmental risk assessment protocols, requires the understanding of the natural fluctuation of the particular biomarker. The aim of this study was to characterize the intrinsic variations of acetylcholinesterase (AChE) activity in tissues of a native freshwater teleost fish to be used as biomarker in toxicity tests, taking into account both seasonal influence and fish size. Specific AChE activity was measured by the method of Ellman et al. (1961) in homogenates of fish anterior section finding a seasonal variability. The highest activity was observed in summer, decreasing significantly below 40% in winter. The annual AChE activity cycle in the anterior section was fitted to a sinusoidal function with a period of 11.2 months. Moreover, an inverse relationship between enzymatic activity and the animal size was established. The results showed that both the fish length and seasonal variability affect AChE activity. AChE activity in fish posterior section showed a similar trend to that in the anterior section, while seasonal variations of the activity in midsection were observed but differences were not statistically significant. In addition, no relationship between AChE and total tissue protein was established in the anterior and posterior sections suggesting that the circannual rhythms observed are AChE-specific responses. Results highlight the importance of considering both the fish size and season variations to reach valid conclusions when AChE activity is employed as neurotoxicity biomarker.

Glyphosate as an acetylcholinesterase inhibitor in Cnesterodon decemmaculatus.

The toxic effect of sublethal concentrations (1, 17.5 and 35 mg L(-1)) of pure glyphosate was evaluated on acetylcholinesterase (AChE) activity in the fish species, Cnesterodon decemmaculatus. Acute bioassays (96 h) under laboratory conditions were conducted and homogenates for each specimen corresponding to the anterior, middle and posterior body sections were performed. Fish survival was 100%, even at the highest concentration tested (35 mg L(-1)), in accordance with the low lethal toxicity reported for glyphosate. However, a significant inhibitory effect on AChE activity was recorded even for the lowest herbicide concentration tested (1 mg L(-1)), in the homogenates corresponding to the anterior body section. The inhibition ranged from 23 to 36%. The analytical determination of glyphosate in assay media by ion chromatography, was used to verify its stability. These results indicate that AChE-a neurotoxicity biomarker-in C. decemmaculatus may be affected by exposure to environmentally relevant concentrations of glyphosate.

Biosolid compost with wood shavings and yard trimmings alleviates stress and improves grain quality in soybean grown in lead polluted soils.

Heavy metals induce stress in plants, thereby affecting growth, crop quality, and food security. Most studies addressing the mitigation of these effects by soil amendment have focused on metals in soils and plant uptake, with there still being a great deal of uncertainty about how amendment application in polluted soils can modify plant stress response and, consequently, yield and food safety. Thus, the aim of this study was to evaluate the effect of biosolid compost amendment on stress response, growth, and lead accumulation in Glycine max, when applied to lead polluted agricultural soils. Soybean was grown in lead polluted soils with 0%, 5%, or 10% (w/w) biosolid compost amendment under controlled conditions in a greenhouse, and the stress response indicators chlorophylls, proteins, sugars, malondialdehyde, glutathione S-transferase activity, carotenes, and the ferric reducing antioxidant power were investigated. In addition, the biomass and lead accumulation in different organs were determined and evaluated with respect to the plant stress. Our results revealed that the addition of 10% biosolid compost improved the grain biomass and appeared to reduce the amount of defective grains, which was related to higher Pb concentrations. Furthermore, 10% compost treatment reduced the stress in plants, leading to a better performance of the photosynthetic system, and with the antioxidant response being positively correlated to Pb accumulation. Lead uptake in plants was decreased by between 35 and 57% after this treatment in comparison with unamended soils. These results indicate that biosolid compost amendment may be an effective way to alleviate Pb uptake and metal stress in soybeans.

Availability of lead in agricultural soils amended with compost of biosolid with wood shavings and yard trimmings.

Lead-polluted agricultural soils are a serious problem for food safety, with organic amendment being a promising mitigation method from the environmental perspective. Therefore, the purpose of this study was to evaluate lead availability and the effectiveness of the application of compost of biosolid with wood shavings and yard trimmings in contaminated soils. The physicochemical (Pb distribution, organic matter, pH, electric conductivity, cation exchange capacity, nitrogen, phosphorus, carbon, carbonates, exchangeable cations, sodium) and biological parameters (the microbial activity obtained by fluorescein diacetate hydrolysis) in Pb-polluted and non-polluted agricultural soils were evaluated after the addition of biosolid with wood shavings and yard trimming compost. Topsoils (lead-polluted and control) were collected in the vicinity of a former battery-recycling plant, amended with compost (0%, 5%, and 10%), and incubated in controlled conditions for 118 days. The results showed that lead availability decreased significantly, and the nutritional quality of the soils increased in the soils amended with 10% of compost. Taken together, the results of the present study indicated that compost amendment could be an effective method for mitigating the negative effects of lead in agricultural soils.