The greening of the Northern Great Plains and its biogeochemical precursors.

Vegetation greenness has increased across much of the global land surface over recent decades. This trend is projected to continue-particularly in northern latitudes-but future greening may be constrained by nutrient availability needed for plant carbon (C) assimilation in response to CO2 enrichment (eCO2 ). eCO2 impacts foliar chemistry and function, yet the relative strengths of these effects versus climate in driving patterns of vegetative greening remain uncertain. Here we combine satellite measurements of greening with a 135 year record of plant C and nitrogen (N) concentrations and stable isotope ratios (δ13 C and δ15 N) in the Northern Great Plains (NGP) of North America to examine N constraints on greening. We document significant greening over the past two decades with the highest proportional increases in net greening occurring in the dries and warmest areas. In contrast to the climate dependency of greening, we find spatially uniform increases in leaf-level intercellular CO2 and intrinsic water use efficiency that track rising atmospheric CO2 . Despite large spatial variation in greening, we find sustained and climate-independent declines in foliar N over the last century. Parallel declines in foliar δ15 N and increases in C:N ratios point to diminished N availability as the likely cause. The simultaneous increase in greening and decline in foliar N across our study area points to increased N use efficiency (NUE) over the last two decades. However, our results suggest that plant NUE responses are likely insufficient to sustain observed greening trends in NGP grasslands in the future.

Multiproxy isotopic analyses of human skeletal material from Rapa Nui: Evaluating the evidence from carbonates, bulk collagen, and amino acids.

OBJECTIVES: Stable isotope ratio analysis of bulk bone collagen dominates research into past diet; however, bone carbonate and compound specific isotope analyses (CSIA) of amino acids provide alternative, yet complementary, lines of evidence toward that same research goal. Together they inform on different aspects of diet, allowing greater certainty in reconstructions. Here we present new data on carbonate isotopes for Rapa Nui and reevaluate prehistoric diet in the context of these new and previously published bulk collagen and CSIA data. MATERIALS AND METHODS: We analyzed carbon isotopes in bone carbonate from 28 prehistoric human teeth from Rapa Nui. These represent a subset of material examined previously for carbon and nitrogen isotope ratios in bulk collagen. We then reevaluate prehistoric diet in light of these and other published data. In addition, we analyzed carbon and nitrogen isotope ratios in 28 modern plant specimens from Rapa Nui to better approximate the isotopic value of the terrestrial endmember. RESULTS: Bulk data suggest a predominantly terrestrial diet, with the amount of marine sources incorporated varying though time. While previously argued to reveal greater amounts of marine consumption, reanalysis of recently published CSIA data suggests this result may relate to the proportion of carbon assimilated rather than consumed. Utilizing models incorporating concentration dependence for estimating dietary proportions results in much lower estimates of marine consumption, in line with findings of the bulk data. DISCUSSION: While these data indicate a larger focus on terrestrial resources, limitations in all forms of analysis make it difficult to determine exact dietary contributions in this mixed system. Better understanding of the complex physiological processes governing isotopic routing and fractionation, and knowledge of appropriate isotopic endmember values are needed to advance this research.

Early human use of anadromous salmon in North America at 11,500 y ago.

Salmon represented a critical resource for prehistoric foragers along the North Pacific Rim, and continue to be economically and culturally important; however, the origins of salmon exploitation remain unresolved. Here we report 11,500-y-old salmon associated with a cooking hearth and human burials from the Upward Sun River Site, near the modern extreme edge of salmon habitat in central Alaska. This represents the earliest known human use of salmon in North America. Ancient DNA analyses establish the species as Oncorhynchus keta (chum salmon), and stable isotope analyses indicate anadromy, suggesting that salmon runs were established by at least the terminal Pleistocene. The early use of this resource has important implications for Paleoindian land use, economy, and expansions into northwest North America.

Compound-specific amino acid δ15 N values in archaeological shell: Assessing diagenetic integrity and potential for isotopic baseline reconstruction.

RATIONALE: Reconstructing stable isotope (SI) ratios at the base of paleo-food webs is often challenging. For coastal systems, the SI ratios of organic matter in archeological shell represents a possible solution, providing a direct record of primary consumer SI ratios in the littoral zone. However, shell is often porous, with organic compounds susceptible to diagenetic alteration or contamination. If molecular isotopic information is well preserved, compound-specific amino acid isotope analysis (CSI-AA) has the potential to provide direct proxies for baseline SI ratios, bypassing many contamination issues, and to allow assessment of the diagenetic state. METHODS: We collected shell from both archeological middens and nearby littoral zones in coastal Alaska, and used a simple organic extraction approach based on decalcification with sequential weak HCl additions to liberate organic material. We measured CSI-AA patterns, molar AA distributions, and the CSI-AA degradation parameter (ΣV), in the context of bulk SI ratios in fossil shell, modern shell, and soft tissue from five common taxa (urchin, limpet, mussel, periwinkle, chiton). RESULTS: CSI-AA patterns in both soft tissue and shell were consistent with primary consumers, and were indistinguishable in most modern and fossil shell pairs, showing that amino acid δ15 N values can be well preserved in archeological shell. AA molar distributions were also similar, although most fossil shell was enriched in Asx and Gly. Comparison between CSI-AA results from modern specimens confirmed that the source AA group (tracking isotopic baselines) are transferred without substantial modification into the shell record. In contrast, the Trophic AA group had elevated δ15 N values in shell versus soft tissue for all taxa examined, suggesting that a correction factor will be required for any CSI-AA proxies using these AAs. CONCLUSIONS: Overall, this new data indicates that the CSI-AA analysis of fossil shell represents a promising new approach to determining isotopic baselines in coastal paleo-ecosystems.

Maternal exposure to carbamazepine at environmental concentrations can cross intestinal and placental barriers.

Psychoactive pharmaceuticals have been found as teratogens at clinical dosage during pregnancy. These pharmaceuticals have also been detected in minute (ppb) concentrations in drinking water in the US, and are environmental contaminants that may be complicit in triggering neurological disorders in genetically susceptible individuals. Previous studies have determined that psychoactive pharmaceuticals (fluoxetine, venlafaxine and carbamazepine) at environmentally relevant concentrations enriched sets of genes regulating development and function of the nervous system in fathead minnows. Altered gene sets were also associated with potential neurological disorders, including autism spectrum disorders (ASD). Subsequent in vitro studies indicated that psychoactive pharmaceuticals altered ASD-associated synaptic protein expression and gene expression in human neuronal cells. However, it is unknown if environmentally relevant concentrations of these pharmaceuticals are able to cross biological barriers from mother to fetus, thus potentially posing risks to nervous system development. The main objective of this study was to test whether psychoactive pharmaceuticals (fluoxetine, venlafaxine, and carbamazepine) administered through the drinking water at environmental concentrations to pregnant mice could reach the brain of the developing embryo by crossing intestinal and placental barriers. We addressed this question by adding (2)H-isotope labeled pharmaceuticals to the drinking water of female mice for 20 days (10 pre-and 10 post-conception days), and quantifying (2)H-isotope enrichment signals in the dam liver and brain of developing embryos using isotope ratio mass spectrometry. Significant levels of (2)H enrichment was detected in the brain of embryos and livers of carbamazepine-treated mice but not in those of control dams, or for fluoxetine or venlafaxine application. These results provide the first evidence that carbamazepine in drinking water and at typical environmental concentrations is transmitted from mother to embryo. Our results, combined with previous evidence that carbamazepine may be associated with ASD in infants, warrant the closer examination of psychoactive pharmaceuticals in drinking water and their potential association with neurodevelopmental disorders.

Centennial-scale fluctuations and regional complexity characterize Pacific salmon population dynamics over the past five centuries.

Observational data from the past century have highlighted the importance of interdecadal modes of variability in fish population dynamics, but how these patterns of variation fit into a broader temporal and spatial context remains largely unknown. We analyzed time series of stable nitrogen isotopes from the sediments of 20 sockeye salmon nursery lakes across western Alaska to characterize temporal and spatial patterns in salmon abundance over the past ∼500 y. Although some stocks varied on interdecadal time scales (30- to 80-y cycles), centennial-scale variation, undetectable in modern-day catch records and survey data, has dominated salmon population dynamics over the past 500 y. Before 1900, variation in abundance was clearly not synchronous among stocks, and the only temporal signal common to lake sediment records from this region was the onset of commercial fishing in the late 1800s. Thus, historical changes in climate did not synchronize stock dynamics over centennial time scales, emphasizing that ecosystem complexity can produce a diversity of ecological responses to regional climate forcing. Our results show that marine fish populations may alternate between naturally driven periods of high and low abundance over time scales of decades to centuries and suggest that management models that assume time-invariant productivity or carrying capacity parameters may be poor representations of the biological reality in these systems.

1,500 year quantitative reconstruction of winter precipitation in the Pacific Northwest.

Multiple paleoclimate proxies are required for robust assessment of past hydroclimatic conditions. Currently, estimates of drought variability over the past several thousand years are based largely on tree-ring records. We produced a 1,500-y record of winter precipitation in the Pacific Northwest using a physical model-based analysis of lake sediment oxygen isotope data. Our results indicate that during the Medieval Climate Anomaly (MCA) (900-1300 AD) the Pacific Northwest experienced exceptional wetness in winter and that during the Little Ice Age (LIA) (1450-1850 AD) conditions were drier, contrasting with hydroclimatic anomalies in the desert Southwest and consistent with climate dynamics related to the El Niño Southern Oscillation (ENSO) and the Pacific Decadal Oscillation (PDO). These findings are somewhat discordant with drought records from tree rings, suggesting that differences in seasonal sensitivity between the two proxies allow a more compete understanding of the climate system and likely explain disparities in inferred climate trends over centennial timescales.

Drought variability in the Pacific Northwest from a 6,000-yr lake sediment record.

We present a 6,000-yr record of changing water balance in the Pacific Northwest inferred from measurements of carbonate δ(18)O and grayscale on a sediment core collected from Castor Lake, Washington. This subdecadally resolved drought record tracks the 1,500-yr tree-ring-based Palmer Drought Severity Index reconstructions of Cook et al. [Cook ER, Woodhouse CA, Eakin CM, Meko DM, Stahle DW (2004) Science 306:1015-1018] in the Pacific Northwest and extends our knowledge back to 6,000 yr B.P. The results demonstrate that low-frequency drought/pluvial cycles, with occasional long-duration, multidecadal events, are a persistent feature of regional climate. Furthermore, the average duration of multidecadal wet/dry cycles has increased since the middle Holocene, which has acted to increase the amplitude and impact of these events. This is especially apparent during the last 1,000 yr. We suggest these transitions were driven by changes in the tropical and extratropical Pacific and are related to apparent intensification of the El Niño Southern Oscillation over this interval and its related effects on the Pacific Decadal Oscillation. The Castor Lake record also corroborates the notion that the 20th century, prior to recent aridity, was a relatively wet period compared to the last 6,000 yr. Our findings suggest that the hydroclimate response in the Pacific Northwest to future warming will be intimately tied to the impact of warming on the El Niño Southern Oscillation.

Weather and leaf age separately contribute to temporal shifts in phyllosphere community structure and composition.

Microbial communities living on plant leaves can positively or negatively influence plant health and, by extension, can impact whole ecosystems. Most research into the leaf microbiome consists of snapshots, and little is known about how microbial communities change over time. Weather and host physiological characteristics change over time and are often collinear with other time-varying factors, such as substrate availability, making it difficult to separate the factors driving microbial community change. We leveraged repeated measures over the course of an entire year to isolate the relative importance of environmental, host physiological, and substrate age-related factors on the assembly, structure, and composition of leaf-associated fungal communities. We applied both culturing and sequencing approaches to investigate these communities, focusing on a foundational, widely-distributed plant of conservation concern: basin big sagebrush ( Artemisia tridentata subsp. tridentata ). We found that changes in alpha diversity were independently affected by the age of a community and the air temperature. Surprisingly, total fungal abundance and species richness were not positively correlated and responded differently, sometimes oppositely, to weather. With regard to beta diversity, communities were more similar to each other across similar leaf ages, air temperatures, leaf types, and δ 13 C stable isotope ratios. Nine different genera were differentially abundant with air temperature, δ 13 C, leaf type, and leaf age, and a set of 20 genera were continuously present across the year. Our findings highlight the necessity for longer-term, repeated sampling to parse drivers of temporal change in leaf microbial communities. Open Research Statement: All ITS DNA amplicon sequence raw data are deposited in the NCBI Sequence Read Archive (SRA), BioProject number PRJNA1107252, data will be released upon publication. All community data, metadata, taxonomic data, and R code necessary to reproduce these results are deposited in the GitHub repository archived on Zenodo: 10.5281/zenodo.11106439.

A stable isotope (δ13C and δ15N) perspective on human diet on Rapa Nui (Easter Island) ca. AD 1400-1900.

Ecological and environmental evidence suggests that Rapa Nui was among the most marginally habitable islands in Eastern Polynesia, with only a fraction of the biotic diversity found on archipelagos to the west, and capable of sustaining many fewer cultigens traditionally transported by Polynesian colonizers. However, archaeological evidence for human dietary adaptations under such restrictions is limited. Little is known about the particulars of the subsistence base and dietary changes on Rapa Nui that may be associated with a hypothesized late prehistoric decline in the quality and diversity of food sources. To better understand prehistoric Rapa Nui diet we examined stable carbon and nitrogen isotope compositions of human teeth along with archaeological faunal material thought to comprise the Rapa Nui food web. Our results indicate that contrary to previous zooarchaeological studies diet was predominantly terrestrial throughout the entire sequence of occupation, with reliance on rats, chickens and C3 plants. While a few individuals may have had access to higher trophic level marine resources, this is evident only later in time (generally post-AD 1600). A decline in (15)N through time was observed, and may be attributed to declines in available terrestrial proteins; however, presently we cannot rule out the effect of changing soil and plant baseline δ(15)N. Our results also suggest differential access to higher trophic level marine resources among contemporaneous populations, but more research is required to clarify this observation.

Freshwater and anadromous fishing in Ice Age Beringia.

While freshwater and anadromous fish have been critical economic resources for late prehistoric and modern Native Americans, the origin and development of fishing is not well understood. We document the earliest known human use of freshwater and anadromous fish in North America by 13,000 and 11,800 years ago, respectively, from primary anthropogenic contexts in central Alaska (eastern Beringia). Fish use appears conditioned by broad climatic factors, as all occurrences but one are within the Younger Dryas chronozone. Earlier Bølling-Allerød and later early Holocene components, while exhibiting similar organic preservation, did not yield evidence of fishing, suggesting that this was a response to changing environmental factors, perhaps reductions in higher ranked resources such as large terrestrial mammals. Late Pleistocene and recent Indigenous peoples harvested similar fish taxa in the region (salmon, burbot, whitefish, and pike). We characterize late Pleistocene fishing in interior Beringia as an important element of broad-spectrum foraging rather than the intensive communal fishing and storage common among recent peoples.

Multiscale responses and recovery of soils to wildfire in a sagebrush steppe ecosystem.

Ecological theory predicts a pulse disturbance results in loss of soil organic carbon and short-term respiration losses that exceed recovery of productivity in many ecosystems. However, fundamental uncertainties remain in our understanding of ecosystem recovery where spatiotemporal variation in structure and function are not adequately represented in conceptual models. Here we show that wildfire in sagebrush shrublands results in multiscale responses that vary with ecosystem properties, landscape position, and their interactions. Consistent with ecological theory, soil pH increased and soil organic carbon (SOC) decreased following fire. In contrast, SOC responses were slope aspect and shrub-microsite dependent, with a larger proportional decrease under previous shrubs on north-facing aspects compared to south-facing ones. In addition, respiratory losses from burned aspects were not significantly different than losses from unburned aspects. We also documented the novel formation of soil inorganic carbon (SIC) with wildfire that differed significantly with aspect and microsite scale. Whereas pH and SIC recovered within 37 months post-fire, SOC stocks remained reduced, especially on north-facing aspects. Spatially, SIC formation was paired with reduced respiration losses, presumably lower partial pressure of carbon dioxide (pCO2), and increased calcium availability, consistent with geochemical models of carbonate formation. Our findings highlight the formation of SIC after fire as a novel short-term sink of carbon in non-forested shrubland ecosystems. Resiliency in sagebrush shrublands may be more complex and integrated across ecosystem to landscape scales than predicted based on current theory.

Using stable isotopes to infer stock-specific high-seas distribution of maturing sockeye salmon in the North Pacific.

The stock-specific distribution of maturing salmon in the North Pacific has been a persistent information gap that has prevented us from determining the ocean conditions experienced by individual stocks. This continues to impede understanding of the role of ocean conditions in stock-specific population dynamics. We assessed scale archives for 17 sockeye salmon (Oncorhynchus nerka) stocks covering the entire North Pacific, from the Columbia River (Washington State and British Columbia) to Kamchatka Peninsula (Russia), to infer salmon locations during their last growing season before returning to their spawning grounds. The approach used, first pioneered in salmon stocks in the Atlantic, relies on the relationship between temporal changes in δ13C in salmon scales and sea surface temperature to estimate salmon distribution based on correlation strength. An advantage of this approach is that it does not require fish sampling at sea, but relies on existing fishery agency collections of salmon scales. Significant correlations were found for 7 of the stocks allowing us to propose plausible feeding grounds. Complementary information from δ15N, historical tagging studies, and connectivity analysis were used to further refine distribution estimates. This study is a first step toward estimating stock-specific distributions of salmon in the North Pacific and provides a basis for the application of the approach to other salmon scale archives. This information has the potential to improve our ability to relate stock dynamics to ocean conditions, ultimately enabling improved stock management. For example, our estimated distributions of Bristol Bay and NE Pacific stocks demonstrated that they occupy different areas with a number of the former being distributed in the high productivity shelf waters of the Aleutian Islands and Bering Sea. This may explain why these stocks seem to have responded differently to changes in ocean conditions, and the long-term trend of increased productivity of Bristol Bay sockeye.

Differentiating salmonid migratory ecotypes through stable isotope analysis of collagen: Archaeological and ecological applications.

The ability to distinguish between different migratory behaviours (e.g., anadromy and potamodromy) in fish can provide important insights into the ecology, evolution, and conservation of many aquatic species. We present a simple stable carbon isotope (δ13C) approach for distinguishing between sockeye (anadromous ocean migrants) and kokanee (potamodromous freshwater residents), two migratory ecotypes of Oncorhynchus nerka (Salmonidae) that is applicable throughout most of their range across coastal regions of the North Pacific Ocean. Analyses of kokanee (n = 239) and sockeye (n = 417) from 87 sites spanning the North Pacific (Russia to California) show that anadromous and potamodromous ecotypes are broadly distinguishable on the basis of the δ13C values of their scale and bone collagen. We present three case studies demonstrating how this approach can address questions in archaeology, archival, and conservation research. Relative to conventional methods for determining migratory status, which typically apply chemical analyses to otoliths or involve genetic analyses of tissues, the δ13C approach outlined here has the benefit of being non-lethal (when applied to scales), cost-effective, widely available commercially, and should be much more broadly accessible for addressing archaeological questions since the recovery of otoliths at archaeological sites is rare.

Ancient Beringian paleodiets revealed through multiproxy stable isotope analyses.

The earliest Native Americans have often been portrayed as either megafaunal specialists or generalist foragers, but this debate cannot be resolved by studying the faunal record alone. Stable isotope analysis directly reveals the foods consumed by individuals. We present multi-tissue isotope analyses of two Ancient Beringian infants from the Upward Sun River site (USR), Alaska (~11,500 years ago). Models of fetal bone turnover combined with seasonally-sensitive taxa show that the carbon and nitrogen isotope composition of USR infant bone collagen reflects maternal diets over the summer. Using comparative faunal isotope data, we demonstrate that although terrestrial sources dominated maternal diets, salmon was also important, supported by carbon isotope analysis of essential amino acids and bone bioapatite. Tooth enamel samples indicate increased salmon use between spring and summer. Our results do not support either strictly megafaunal specialists or generalized foragers but indicate that Ancient Beringian diets were complex and seasonally structured.

Historical analysis of salmon-derived polychlorinated biphenyls (PCBs) in lake sediments.

Several recent studies have highlighted the importance of salmon as a means to deliver biomagnifying contaminants to nursery lakes. There is a lack of studies, however, which demonstrate empirically how this source has varied through time. This is of great significance because past salmon-derived contaminant loading was potentially greater than it is today. By analyzing radiometrically dated sediment cores collected from ten lakes in Alaska and British Columbia (B.C.), we relate historical numbers of sockeye salmon spawners to SigmaPCB concentrations and delta(15)N values (a paleolimnological proxy for past salmon-derived nitrogen) in the sediments. The results confirm that sockeye salmon have provided an important route for PCBs to enter the lakes in the past, a finding that is especially evident when the data of all lakes are pooled. Significant relationships between sockeye salmon numbers and delta(15)N, as well as SigmaPCB concentrations and delta(15)N in sediments, were also found. However, it is difficult to establish relationships between salmon numbers, SigmaPCBs and delta(15)N in individual lakes. This may be due to a number of factors which may influence contaminant loadings to the lakes. The factors include: a) changing salmon contaminant loads over time resulting from a lag in the upper ocean reservoir and/or changing salmon feeding locations; b) greater importance of atmospheric transport in lakes with relatively low salmon returns; and c) increased PCB scavenging due to higher algae productivity in the lakes in recent years.

Tracing salmon-derived nutrients and contaminants in freshwater food webs across a pronounced spawner density gradient.

Many have demonstrated that anadromous Pacific salmon are significant vectors of nutrients from the ocean to freshwaters. Recently. however, it has been recognized that salmon spawners also input significant quantities of contaminants. The objectives of this paper are to delineate the extent to which salmon-derived nutrients are integrated into the freshwater food web using delta(15)N and delta(13)C and to assess the influence of the salmon pathway in the accumulation of contaminants in rainbow trout (Oncorhynchus mykiss). We found that the delta(15)N and delta(13)C of food web components were related positively and significantly to sockeye salmon (Oncorhynchus nerka) spawner density. Contaminant concentrations in rainbow trout also positively and significantly were related to sockeye salmon spawner density. These data suggest that the anadromous salmon nutrient and contaminant pathways are related and significantly impact the contaminant burden of resident fish.

Recent physical connections may explain weak genetic structure in western Alaskan chum salmon (Oncorhynchus keta) populations.

Low genetic divergence at neutral loci among populations is often the result of high levels of contemporary gene flow. Western Alaskan summer-run chum salmon (Oncorhynchus keta) populations demonstrate weak genetic structure, but invoking contemporary gene flow as the basis for the low divergence is problematic because salmon home to their natal streams and some of the populations are thousands of kilometers apart. We used genotypes from microsatellite and single nucleotide polymorphism loci to investigate alternative explanations for the current genetic structure of chum salmon populations from western Alaska. We also estimated current levels of gene flow among Kuskokwim River populations. Our results suggest that weak genetic structure is best explained by physical connections that occurred after the Holocene Thermal Maximum among the Yukon, Kuskokwim, and Nushagak drainages that allowed gene flow to occur among now distant populations.

A coherent signature of anthropogenic nitrogen deposition to remote watersheds of the Northern Hemisphere.

Humans have more than doubled the amount of reactive nitrogen (Nr) added to the biosphere, yet most of what is known about its accumulation and ecological effects is derived from studies of heavily populated regions. Nitrogen (N) stable isotope ratios ((15)N:(14)N) in dated sediments from 25 remote Northern Hemisphere lakes show a coherent signal of an isotopically distinct source of N to ecosystems beginning in 1895 ± 10 years (±1 standard deviation). Initial shifts in N isotope composition recorded in lake sediments coincide with anthropogenic CO(2) emissions but accelerate with widespread industrial Nr production during the past half century. Although current atmospheric Nr deposition rates in remote regions are relatively low, anthropogenic N has probably influenced watershed N budgets across the Northern Hemisphere for over a century.

Concentrations and fluxes of salmon-derived polychlorinated biphenyls (PCBs) in lake sediments.

Fourteen sediment cores were collected from 10 lakes spanning a large gradient of sockeye salmon returns (0-40 000 spawners km(-2)) in Alaska and British Columbia in 1995-98 and 2002/03. The cores were analyzed for 210Pb to determine sedimentation rates and focusing factors. Polychlorinated biphenyl (PCBs) concentrations in the surface sediments (0-2 cm) were highly correlated with the number of sockeye salmon returns to each nursery lake. For 2002/03, the correlation between PCB concentration and number of salmon spawners was best with no correction factors applied, but decreased considerably when corrected for sedimentation rates, and was improved again by correcting for sediment focusing. Although sigmaPCB concentrations were similar in 1995-98 and 2002/03, the congener patterns varied. Because salmon are the dominant source of PCBs for most of these lakes, variation in sediment congener pattern likely derives from variation in congener patterns carried by the salmon. Overall, total PCB input by salmon has remained relatively constant since 1995. Unlike temperate Great Lakes contaminant studies, the North American west-coast lakes dominated by salmon bio-transport showed no sign of recent decrease in PCBs.