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1.
Prog Urol ; 27(17): 1091-1097, 2017 Dec.
Artigo em Francês | MEDLINE | ID: mdl-28964687

RESUMO

OBJECTIVE: Treatment with transcutaneous posterior tibial neurostimulation (NTPT) has been shown to be effective in the treatment of overactive bladder (OAB), but its outcomes in diabetic patients have never been assessed. The aim of this study was to compare the efficacy of NTPT in diabetic OAB patients and in OAB patients without diabetes. METHODS: A single-center prospective study included all patients treated with NTPT for lower urinary tract storage symptoms between 2012 and 2016. The primary endpoint was symptoms improvement≥50% assessed using a Visual Analog Scale (VAS) two months after starting NTPT. Treatment consisted in a daily 20-minute NTPT single-session. The secondary endpoints were lower urinary tract symptoms reported by bladder diary, the Urinary Symptom Profile, the impact on mood and on daily activities. RESULTS: Seventy-one patients were included, 10 of whom were diabetic. The efficacy rate (EVA>50%) was not significantly different in the diabetic group (70% vs. 44.1%, P=0.17), like the mean EVA efficacy was similar in both groups (4/10 vs. 4/10, P=0.98). OAB USP sub-score diminished significantly in both groups at 2 months (-3 points in the diabetic group; -1.9 points in the non-diabetic group; P=0.03 and P<0.0001, respectively). There was no significant difference between the groups, except for the rate of patients who stopped treatment at 6 months, higher in diabetic patients (100% vs. 63.5%, P=0.04). CONCLUSION: The functional outcomes of NTPT appear to be similar in the treatment of OAB in diabetic patients and in non-diabetic patients. LEVEL OF EVIDENCE: 4.


Assuntos
Complicações do Diabetes/terapia , Nervo Tibial , Estimulação Elétrica Nervosa Transcutânea , Bexiga Urinária Hiperativa/terapia , Idoso , Complicações do Diabetes/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Bexiga Urinária Hiperativa/etiologia
2.
Prog Urol ; 26(4): 245-53, 2016 Mar.
Artigo em Francês | MEDLINE | ID: mdl-26452712

RESUMO

OBJECTIVES: Specify urinary functional impairment associated with diabetic pathology. Propose guidance for screening, monitoring of clinical signs of lower urinary tract (LUTS) and describe the specifics of the urological treatment of patients. METHODS: A review of literature using PubMed library was performed using the following keywords alone or in combination: "diabetes mellitus", "diabetic cystopathy", "overactive bladder", "bladder dysfunction", "urodynamics", "nocturia". RESULTS: LUTS are more common in the diabetic population with an estimated prevalence between 37 and 70 %, and are probably underevaluated in routine practice. They are heterogeneous and are frequently associated with other diabetic complications. Both storage and voiding symptoms can coexist. Despite a major evaluation in the literature, no recommendation supervises the assessment and management of LUTS in this specific population. An annual screening including medical history, bladder and kidney ultrasound and post-void residual measurement is required in the follow-up of diabetic patients. Specific urologial referral and urodynamic investigations will be performed according to the findings of first-line investigations. The type of bladder dysfunction, the risk of urinary tract infections and dysautonomia should be considered in the specific urological management of these patients. CONCLUSION: Diabetes mellitus significantly impacts on the lower urinary tract function. A screening of LUTS is required as well as other complications of diabetes. The management of LUTS must take into consideration the specific risks of the diabetic patient regarding the loss of bladder contractility, the possibility of dysautonomia and infectious complications.


Assuntos
Complicações do Diabetes/complicações , Sintomas do Trato Urinário Inferior/etiologia , Doenças da Bexiga Urinária/etiologia , Algoritmos , Complicações do Diabetes/diagnóstico , Complicações do Diabetes/terapia , Humanos , Sintomas do Trato Urinário Inferior/diagnóstico , Sintomas do Trato Urinário Inferior/terapia , Doenças da Bexiga Urinária/diagnóstico , Doenças da Bexiga Urinária/terapia
3.
medRxiv ; 2024 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-39148830

RESUMO

Background: Mood and anxiety disorders are highly prevalent and comorbid worldwide, with variability in symptom severity that fluctuates over time. Digital phenotyping, a growing field that aims to characterize clinical, cognitive and behavioral features via personal digital devices, enables continuous quantification of symptom severity in the real world, and in real-time. Methods: In this study, N=114 individuals with a mood or anxiety disorder (MA) or healthy controls (HC) were enrolled and completed 30-days of ecological momentary assessments (EMA) of symptom severity. Novel real-world measures of anxiety, distress and depression were developed based on the established Mood and Anxiety Symptom Questionnaire (MASQ). The full MASQ was also completed in the laboratory (in-lab). Additional EMA measures related to extrinsic and intrinsic motivation, and passive activity data were also collected over the same 30-days. Mixed-effects models adjusting for time and individual tested the association between real-world symptom severity EMA and the corresponding full MASQ sub-scores. A graph theory neural network model (DEPNA) was applied to all data to estimate symptom interactions. Results: There was overall good adherence over 30-days (MA=69.5%, HC=71.2% completion), with no group difference (t(58)=0.874, p=0.386). Real-world measures of anxiety/distress/depression were associated with their corresponding MASQ measure within the MA group (t's > 2.33, p's < 0.024). Physical activity (steps) was negatively associated with real-world distress and depression (IRRs > 0.93, p's ≤ 0.05). Both intrinsic and extrinsic motivation were negatively associated with real-world distress/depression (IRR's > 0.82, p's < 0.001). DEPNA revealed that both extrinsic and intrinsic motivation significantly influenced other symptom severity measures to a greater extent in the MA group compared to the HC group (extrinsic/intrinsic motivation: t(46) = 2.62, p < 0.02, q FDR < 0.05, Cohen's d = 0.76; t(46) = 2.69, p < 0.01, q FDR < 0.05, Cohen's d = 0.78 respectively), and that intrinsic motivation significantly influenced steps (t(46) = 3.24, p < 0.003, q FDR < 0.05, Cohen's d = 0.94). Conclusions: Novel real-world measures of anxiety, distress and depression significantly related to their corresponding established in-lab measures of these symptom domains in individuals with mood and anxiety disorders. Novel, exploratory measures of extrinsic and intrinsic motivation also significantly related to real-world mood and anxiety symptoms and had the greatest influencing degree on patients' overall symptom profile. This suggests that measures of cognitive constructs related to drive and activity may be useful in characterizing phenotypes in the real-world.

4.
Acta Chir Belg ; 113(6): 455-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24494476

RESUMO

We present a new surgical technique where skin closure was obtained in the treatment of a thoracolumbar myelomeningocele by mobilization of four muscle flaps, two reverse latissimus dorsi muscle flaps combined with the caudal portions of two trapezius muscle flaps.


Assuntos
Meningomielocele/cirurgia , Procedimentos Neurocirúrgicos/métodos , Procedimentos de Cirurgia Plástica/métodos , Retalhos Cirúrgicos , Feminino , Humanos , Recém-Nascido , Vértebras Lombares , Meningomielocele/diagnóstico , Diagnóstico Pré-Natal , Vértebras Torácicas
5.
Rev Med Brux ; 34(4): 287-90, 2013 Sep.
Artigo em Francês | MEDLINE | ID: mdl-24195242

RESUMO

The use of aesthetic medicine and surgery is increasingly popular, and becomes a true phenomenon of society. Many women and men are asking for such treatments. A large proportion of the population carry the idea that this branch of medicine is a true consumer product. The acts of aesthetic medicine and surgery are not without consequences. They produce important changes in the human body, and carry risks of complications that must be taken into account. The overrated media interest of this subject produce commercial drifts that act against the general health of the patients. The invasive acts of medical aesthetics must be placed in a precise legal and ethical framework to protect the patients. A project of a new Belgian law is on the way, awaiting for publication in "Le Moniteur": this law (proposed by Senator Dominique Tilmans) clarifies the competences required for performing non-surgical aesthetic treatments and specific aesthetic invasive treatments. Other projects of law are being studied, and will concern publicity, information of the public, and rules of private clinics where aesthetic invasive acts are performed. Recent international news have shown, with the PIP breast prosthesis scandal, that surveillance of the medical aesthetic field is mandatory. To provide a better protection of patients, the legislator has decided legislate over the subject.


Assuntos
Ética Médica , Cirurgia Plástica/ética , Instituições de Assistência Ambulatorial/ética , Instituições de Assistência Ambulatorial/legislação & jurisprudência , Feminino , Humanos , Legislação Médica/tendências , Masculino , Cirurgia Plástica/legislação & jurisprudência
6.
Eur Ann Otorhinolaryngol Head Neck Dis ; 137(1): 59-60, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31178429

RESUMO

INTRODUCTION: Robotic thyroidectomy (RT) has been reported to achieve satisfactory results. However, its long-term oncological results have not been validated. The use of RT to treat thyroid cancer comprises specific risks that are not encountered with open thyroidectomy (OT). CASE SUMMARY: We report the case of a patient operated by two-stage RT for stage pT3a follicular carcinoma, followed by tumour seeding along the incision despite adjuvant Iodine-131 therapy and external beam radiotherapy. DISCUSSION: This case illustrates the limitations and insufficient perspective of RT for thyroid cancer, with the recent discovery of a risk of tumour seeding along the incision. Rigorous patient selection should help to avoid this risk of tumour seeding.


Assuntos
Adenocarcinoma Folicular/cirurgia , Procedimentos Cirúrgicos Robóticos , Neoplasias da Glândula Tireoide/cirurgia , Tireoidectomia/métodos , Axila , Feminino , Humanos , Masculino , Procedimentos Cirúrgicos Robóticos/métodos
7.
Mol Cell Biol ; 12(4): 1507-14, 1992 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-1549108

RESUMO

Four ADR1c mutations that occur close to Ser-230 of the Saccharomyces cerevisiae transcriptional activator ADR1 and which greatly enhance the ability of ADR1 to activate ADH2 expression under glucose-repressed conditions have been shown to reduce or eliminate cyclic AMP-dependent protein kinase (cAPK) phosphorylation of Ser-230 in vitro. In addition, unregulated cAPK expression in vivo blocks ADH2 depression in an ADR1-dependent fashion in which ADR1c mutations display decreased sensitivity to unregulated cAPK activity. Taken together, these data have suggested that ADR1c mutations enhance ADR1 activity by blocking cAPK phosphorylation and inactivation of Ser-230. We have isolated and characterized an additional 17 ADR1c mutations, defining 10 different amino acid changes, that were located in the region defined by amino acids 227 through 239 of ADR1. Three observations, however, indicate that the ADR1c phenotype is not simply equivalent to a lack of cAPK phosphorylation. First, only some of these newly isolated ADR1c mutations affected the ability of yeast cAPK to phosphorylate corresponding synthetic peptides modeled on the 222 to 234 region of ADR1 in vitro. Second, we observed that strains lacking cAPK activity did not display enhanced ADH2 expression under glucose growth conditions. Third, when Ser-230 was mutated to a nonphosphorylatable residue, lack of cAPK activity led to a substantial increase in ADH2 expression under glucose-repressed conditions. Thus, while cAPK controls ADH2 expression and ADR1 is required for this control, cAPK acts by a mechanism that is independent of effects on ADR1 Ser-230. It was also observed that deletion of the ADR1c region resulted in an ADR1c phenotype. The ADR1c region is, therefore, involved in maintaining ADR1 in an inactive form. ADR1c mutations may block the binding of a repressor to ADR1 or alter the structure of ADR1 so that transcriptional activation regions become unmasked.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Proteínas Fúngicas/genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Fatores de Transcrição/genética , Transcrição Gênica , Sequência de Aminoácidos , Análise Mutacional de DNA , Proteínas de Ligação a DNA/genética , Modelos Genéticos , Dados de Sequência Molecular , Peptídeos/síntese química , Peptídeos/metabolismo , Fenótipo , Fosforilação , Proteínas Quinases/metabolismo , Processamento de Proteína Pós-Traducional , Especificidade por Substrato
8.
Acta Chir Belg ; 107(4): 424-8, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17966539

RESUMO

Post-surgical pyoderma gangrenosum (PSPG) represents a specific entity: it shares some clinical aspects of pyoderma gangrenosum (PG), but has a series of its own features. In this paper, two cases of PSPG associated with breast surgery are presented: their analysis, combined with a review of the few other cases reported in the literature, show the particular clinical presentation and evolution of this condition. Firstly, the onset of PSPG follows a sequence. After an apparently normal evolution of scar formation following a surgical procedure, the scar presents with many small dehiscences, which will progressively coalesce to some larger areas of wound ulceration, with no visible granulation tissue. Secondly, the delay between surgery and the beginning of symptoms is variable, from 4 days to sometimes 6 weeks. The process will evolve well beyond what would be expected for the initial wound that was created by surgery, with no self-limitation. The skin ulcerations become larger, despite any local treatment or antibiotics. Thirdly, PSPG can affect any anatomical location with the exception of the nipple-areolar complex. Fourthly, a dramatic response to immunomodulatory drugs will be observed. The pain is an inconstant symptom. Present in the first week after surgery, it can be severe and disproportionate regarding clinical presentation.


Assuntos
Implante Mamário , Complicações Pós-Operatórias , Pioderma Gangrenoso/etiologia , Deiscência da Ferida Operatória , Adulto , Anti-Inflamatórios/uso terapêutico , Feminino , Humanos , Pessoa de Meia-Idade , Pioderma Gangrenoso/tratamento farmacológico
9.
Acta Chir Belg ; 107(2): 155-61, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17515264

RESUMO

BACKGROUND: Pressure ulcers are a frequent complication of bed rest. The development of an efficient and low cost pressure relieving system for the prevention of bed-sores would be of considerable hospital health and economic interest. Our study was designed to determine the effectiveness in pressure-sore prevention of an interface pressure-decreasing mattress, the Kliniplot mattress, used in our institution since 1978. METHODS: In a prospective randomised controlled 7-month clinical trial we compared the Kliniplot mattress with our standard hospital mattress in 1729 patients admitted to medical and surgical departments (neurology, cardiology, oncology-haematology, neurosurgery, thoracic surgery and orthopaedic surgery). Two groups (Klinipot mattress and standard hospital mattress) were monitored for the prevention of pressure sores. The patients were evaluated on a daily basis from their admission until the eventual occurrence of a bed-sore. Patients' characteristics and pressure-sore risk factors were similar at the baseline in both groups. Patients presenting with a pressure sore at the time of admission were excluded. RESULTS: Forty-two of the 1729 patients (2.4%) who entered the study developed at least one pressure sore. Twenty-one of the 657 patients (3.2%) nursed on the Kliniplot mattress, and 21 of the 1072 patients (1.9%) on the standard mattress developed bed-sores (p = 0.154). The median time for the occurrence of pressure sores was 31 days (range 6-87) with the Kliniplot mattress and 18 days (range 2 to 38) with the standard mattress (p < 0.001). The risk categories for developing bed-sores using the modified Ek's scale were no different at the baseline between both groups (p = 0.764). The severity of the pressure sores was no different between both groups (p = 0.918). CONCLUSIONS: Our results show that the occurrence of pressure sores is not reduced but is delayed when patients are nursed on a Kliniplot pressure-decreasing mattress.


Assuntos
Leitos , Hospitalização , Úlcera por Pressão/prevenção & controle , Bélgica/epidemiologia , Desenho de Equipamento , Humanos , Úlcera por Pressão/epidemiologia , Estudos Prospectivos , Índice de Gravidade de Doença , Fatores de Tempo
10.
Vet Comp Oncol ; 15(4): 1513-1526, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28150446

RESUMO

The modified Glasgow Prognostic Score (mGPS) assigns a numerical value (0-2) from pre-treatment serum concentrations of C-reactive protein (CRP) and albumin to predict patient outcome. CRP and albumin were evaluated in 77 untreated dogs with lymphoma to determine the relationship of mGPS to clinicopathological parameters and whether it could predict progression-free (PFS) and overall survival (OS) in treated dogs. mGPS distribution was significantly associated with clinical stage, substage b, weight loss, gastrointestinal disturbances and lethargy at presentation. On univariate analysis, mGPS was significantly associated with OS and PFS, with shorter median survival times for mGPS 2 compared to mGPS 0 and 1 combined. Hypoalbuminaemia significantly reduced OS and PFS, however increased CRP had no effect. Only clinical stage was significantly associated with OS and PFS on both univariate and multivariate analysis. mGPS has potential prognostic value for canine lymphoma , but further studies are needed.


Assuntos
Doenças do Cão/diagnóstico , Linfoma/veterinária , Animais , Proteína C-Reativa/análise , Doenças do Cão/mortalidade , Doenças do Cão/patologia , Cães , Feminino , Linfoma/diagnóstico , Linfoma/mortalidade , Linfoma/patologia , Masculino , Prognóstico , Índice de Gravidade de Doença
12.
Commun Agric Appl Biol Sci ; 71(2 Pt A): 39-48, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-17390771

RESUMO

Regulatory requirements, and in particular phytosanitary quality standards change rapidly. As ACP producers/exporters race to become more competitive, to keep their market share and to satisfay their customers' commercial demands (e.g. EUREP-GAP certification), the need for competent staff who are aware of the company's quality objectives and trained to follow instructions is crucial. Mastering sanitary quality is only possible if matched with a programme to build the skills of companies' human resources. The Pesticide Initiative Programme (PIP), mindful of the importance of making operators autonomous and of training them to monitor EU food safety regulations and technology on their own, has successfully developed a training programme while building a quality network of local/ACP service providers. By building the capacities of ACP experts and then securing their services as trainers, PIP also guarantees companies' access to expertise and the sustainability of their efforts to comply with new EU regulations. The training strategy developed by PIP rests on two pilars: instructor training and collective training. Instructor training consists in reinforcing the technical knowledge of local experts (agronomists, hygienists, etc.) by providing them with active teaching methods. Once the ACP experts have gained enough technical knowledge of the key areas of crop protection--mainly pesticides management--and food safety, and have demonstrated their capacity to train the technical staff of local companies, the PIP has carried out a collective training programme in 2004, 2005 and 2006. To date, more than 130 consultants covering about 15 ACP countries have received instructor training, and more than 700 people have participated in collective and in-company training sessions.


Assuntos
Qualidade de Produtos para o Consumidor , Produtos Agrícolas/química , Produtos Agrícolas/normas , Indústria Alimentícia , Resíduos de Praguicidas/análise , União Europeia , Indústria Alimentícia/legislação & jurisprudência , Indústria Alimentícia/normas , Frutas/química , Frutas/normas , Humanos , Legislação sobre Alimentos , Controle de Qualidade , Verduras/química , Verduras/normas , Recursos Humanos
13.
J Small Anim Pract ; 57(11): 600-609, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27709617

RESUMO

OBJECTIVES: To perform a preliminary study to assess whether single-agent palliative or adjuvant chemotherapy has an impact on objectively measured physical activity in dogs. METHODS: Fifteen dogs with neoplasia (treatment group) wore ActiGraph™ accelerometers for 5-day periods before, during and after receiving single-agent adjuvant or palliative chemotherapy. Mean 5-day total physical activity and time spent in three different intensities of activity (sedentary, light-moderate and vigorous) before, during and after receiving chemotherapy were compared to a group of 15 healthy dogs (control group). Results were also compared within the treatment group across time. RESULTS: Prior to chemotherapy, treated dogs tended to be less active than control dogs. Treatment group dogs were slightly more active at restaging than they were prior to treatment but had similar activity levels to control dogs. Marked effects of chemotherapy on physical activity were not detected. Physical activity was slightly lower in treated dogs during chemotherapy when compared to control dogs but there was a slight increase in physical activity of treated dogs during chemotherapy when compared with pretreatment recordings. There was little change in the mean 5-day total physical activity between treated dogs during chemotherapy and at restaging but a mild decrease in time spent sedentary and increase in time spent in light-moderate activity at this comparison of time points. CLINICAL SIGNIFICANCE: Single-agent adjuvant or palliative chemotherapy had minimal impact on physical activity levels in dogs with neoplasia.


Assuntos
Doenças do Cão/fisiopatologia , Neoplasias/veterinária , Condicionamento Físico Animal , Qualidade de Vida , Acelerometria/veterinária , Animais , Antineoplásicos/uso terapêutico , Estudos de Casos e Controles , Doenças do Cão/tratamento farmacológico , Cães , Feminino , Masculino , Neoplasias/tratamento farmacológico , Neoplasias/fisiopatologia , Estudos Prospectivos
14.
Thyroid ; 15(1): 85-8, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15687829

RESUMO

We report the case of a 36-year old woman with a history of long-term fever associated with a biologic inflammatory syndrome that was not corrected by several courses of corticosteroid treatment. The only remarkable result during previous investigations was the presence of a positive Epstein-Barr virus (EBV) serology. Clinical examination revealed an heterogenous thyroid with a nodule on the right lobe. Serum thyrotropin (TSH) concentration was normal. The levels of antiperoxidase antibodies and thyrocalcitonin were normal. Ultrasound examination of the neck showed a 3-cm hypoechogenous nodule in the right lobe of the thyroid. A total thyroidectomy was performed. Histopathologic findings led to the diagnosis of Riedel's thyroiditis. We observed a dramatic improvement after surgery with absence of fever and normalization of inflammatory parameters. The role of EBV infection in the process of this unusual form of Riedel's thyroiditis is discussed.


Assuntos
Doenças Raras/diagnóstico , Tireoidite/diagnóstico , Adulto , Diagnóstico Diferencial , Técnicas de Diagnóstico por Cirurgia , Infecções por Vírus Epstein-Barr/complicações , Feminino , Humanos , Doenças Raras/patologia , Doenças Raras/cirurgia , Doenças Raras/virologia , Glândula Tireoide/patologia , Tireoidectomia , Tireoidite/patologia , Tireoidite/cirurgia , Tireoidite/virologia
15.
Biol Psychiatry ; 27(3): 304-10, 1990 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-2302439

RESUMO

Brain mapping studies have shown abnormal changes in cerebral blood volume and oxygen consumption, or other neurophysiological abnormalities, in panic disorder (PD) patients. Because of these intriguing reports, we decided to assess the neuroanatomical aspects of patients with PD using magnetic resonance imaging (MRI). We included 31 consecutive cases with a diagnosis of PD according to the DSM-III criteria, and 20 controls. All subjects had to be right-handed and between 20 and 40 years of age. The usual exclusion criteria were applied. We carried out the MRI tests with a General Electric Signa Machine operating at 1.5 Tesla. Over 100 images were obtained per patient with an emphasis on assessing temporal lobe. There were no significant differences in age, gender, or weight between the patients and controls. We found a statistically significant higher number of abnormalities in PD patients (40%), as compared with the controls (10%). The most striking findings were focal abnormalities in the temporal lobes: areas of abnormal signal activity, and asymmetric atrophy of the temporal lobe occurred mostly on the right side. These results implicated the limbic system and may prove to be of particular relevance in panic and phobic disorders. However, the significance of our findings remains unknown and challenging. Further MRI studies in PD will be required for a better understanding of the illness.


Assuntos
Medo/fisiologia , Imageamento por Ressonância Magnética , Transtornos Neurocognitivos/diagnóstico , Pânico/fisiologia , Lobo Temporal/patologia , Adulto , Feminino , Humanos , Masculino , Transtornos Neurocognitivos/patologia , Estudos Prospectivos
16.
Gene ; 184(2): 291-8, 1997 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-9031642

RESUMO

A physical map of the chromosome of Dichelobacter nodosus strain A198 was constructed using the restriction endonucleases EagI and StuI. Mapping data indicated the presence of a single, circular chromosome of 1.54 Mb. The three rRNA operons and the virulence related locus (vrl) were precisely positioned at the junctions of EagI and StuI fragments, and their transcriptional orientations were also determined. Other D. nodosus genes were assigned to specific EagI and StuI fragments. Analysis of the resultant map revealed that the putative virulence genes were not clustered on the chromosome which suggests that the D. nodosus virulence determinants have been acquired gradually and that virulence in D. nodosus is an evolving trait.


Assuntos
Cromossomos Bacterianos , Bactérias Anaeróbias Gram-Negativas/genética , Mapeamento Cromossômico , DNA Bacteriano , DNA Circular , Desoxirribonucleases de Sítio Específico do Tipo II , Eletroforese em Gel de Campo Pulsado , Ligação Genética , Bactérias Anaeróbias Gram-Negativas/patogenicidade , Virulência/genética
17.
Peptides ; 7(4): 645-9, 1986.
Artigo em Inglês | MEDLINE | ID: mdl-3763438

RESUMO

Wistar rats were submitted to portacaval anastomosis (PCA). Control rats were sham-operated and pair-fed (SOPF). After 3 weeks, PCA led to the hypertrophy of right atrium (+50%), left atrium (+67%) and both ventricles (+26%). The response of adenylate cyclase activity to secretin was specifically and markedly decreased in membranes from atria (-51 to 59%) and ventricles (-68 to 69%). These data suggest a decrease in the number of functional secretin receptors in heart considering that: the half-maximal stimulatory secretin concentration was unchanged; glucagon stimulations were unaltered and D,L-isoproterenol stimulations were hardly affected; the Gpp(NH)p-, NaF-, and forskolin-stimulated adenylate cyclase activities were moderately decreased (in ventricles, by 14-28%) or unchanged (in atria).


Assuntos
Adenilil Ciclases/metabolismo , Miocárdio/enzimologia , Derivação Portocava Cirúrgica , Secretina/farmacologia , Animais , Membrana Celular/enzimologia , Colforsina/farmacologia , Feminino , Glucagon/farmacologia , Guanosina Trifosfato/farmacologia , Guanilil Imidodifosfato/farmacologia , Átrios do Coração/enzimologia , Isoproterenol/farmacologia , Cinética , Ratos , Ratos Endogâmicos , Fluoreto de Sódio/farmacologia
18.
AJNR Am J Neuroradiol ; 10(3): 615-7, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2501997

RESUMO

Postmyelography headaches are produced mostly by CSF leakage at the dural puncture site and are therefore largely dependent on the size of the needle used. Our study of 300 consecutive outpatients who had lumbar myelograms performed with 25- and 26-gauge spinal needles shows that the procedure has become virtually innocuous. We recommend that 26-gauge spinal needles be widely adopted as the standard for fluoroscopically controlled intrathecal injections of contrast material.


Assuntos
Mielografia/métodos , Agulhas , Punção Espinal/instrumentação , Adolescente , Adulto , Idoso , Assistência Ambulatorial , Meios de Contraste/administração & dosagem , Estudos de Avaliação como Assunto , Humanos , Pessoa de Meia-Idade , Mielografia/instrumentação , Punção Espinal/métodos
19.
AJNR Am J Neuroradiol ; 16(8): 1605-13; discussion 1614-5, 1995 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-7502962

RESUMO

PURPOSE: To locate the origin of the pain during lumbar diskography by means of a limited intradiskal injection of a local anesthetic. METHODS: Lumbar diskography by the direct central posterior approach was performed in 235 consecutive patients. In 17 patients, severe and persistent low back pain, with unilateral or bilateral radiation to the lower extremities, was provoked by contrast injection into only one disk. One milliliter of 1% lidocaine was then slowly injected in the center of these disks. RESULTS: A 75% to 100% reduction of the low back pain was experienced by 13 patients, and a 75% to 100% reduction of the radiating pain was experienced by 16 patients within 60 seconds after the intradiskal injection of lidocaine. Radiographs demonstrated radial tears through the entire annulus thickness in 16 of 17 disks. CONCLUSION: Our results suggest that, in some patients with low back pain and unilateral or bilateral radiation to the lower extremities, the pain arises from within the disk. In these cases, pain radiating to the lower limb seems to be a referred type and seems unrelated to direct nerve root compression or irritation by a disk fragment in the epidural space.


Assuntos
Deslocamento do Disco Intervertebral/diagnóstico por imagem , Dor Lombar/diagnóstico por imagem , Vértebras Lombares/diagnóstico por imagem , Síndromes de Compressão Nervosa/diagnóstico por imagem , Ciática/diagnóstico por imagem , Raízes Nervosas Espinhais/diagnóstico por imagem , Adulto , Anestesia Local , Feminino , Humanos , Injeções Espinhais , Deslocamento do Disco Intervertebral/tratamento farmacológico , Lidocaína , Dor Lombar/tratamento farmacológico , Vértebras Lombares/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Mielografia , Síndromes de Compressão Nervosa/tratamento farmacológico , Medição da Dor , Ciática/tratamento farmacológico , Raízes Nervosas Espinhais/efeitos dos fármacos , Tomografia Computadorizada por Raios X
20.
Eur J Surg Oncol ; 23(1): 86-7, 1997 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-9066754

RESUMO

Verrucous carcinoma is a rare form of squamous cell carcinoma. It behaves as a slow-growing, locally aggressive and rarely metastasizing tumour. Three clinical cases are presented, with emphasis on clinical presentation, pathological diagnosis and therapeutic approach. The pathogenesis of verrucous carcinoma, including chronic inflammation and human papillomavirus, is also discussed.


Assuntos
Carcinoma Verrucoso , Perna (Membro) , Idoso , Idoso de 80 Anos ou mais , Carcinoma Verrucoso/diagnóstico , Carcinoma Verrucoso/cirurgia , Carcinoma Verrucoso/virologia , Diagnóstico Diferencial , Antepé Humano , Humanos , Masculino , Papillomaviridae
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