Combining ability of tropical and temperate inbred lines of popcorn.

In Brazil, using combining ability of popcorn genotypes to achieve superior hybrids has been unsuccessful because the local genotypes are all members of the same heterotic group. To overcome this constraint, 10 lines (P(1) to P(10)) with different adaptations to tropical or temperate edaphoclimatic environments were used to obtain 45 F(1) hybrids in a complete diallel. These hybrids and three controls were evaluated in two environments in Rio de Janeiro State. Grain yield (GY), popping expansion (PE), plant height (PH), ear height (EH), and days to silking (FL) were evaluated in randomized complete blocks with three replications. Significant differences between genotypes (P

Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays).

We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize hybrid, one sweet corn, one flint-type corn, and three popcorn. Fifteen inter-simple sequence repeat primers were used: (CT)(8)RC, (CT)(8)TG, (GA)(8)T, (GA)(8)YC, (CTC)(5)RC, (GTC)(6), (GA)(6)CC, (GT)(6)CC, (CAC)(3)GC, (AG)(8)YT, (AC)(8)T, (AC)(8)YG, (CT)(8)RG, (GGAT)(3)GA, and (GAA)(6)AA. The annealing temperature and the melting temperature for each primer were estimated using a formula for RW Genes products, or we used the temperatures indicated by the manufacturer (Invitrogen). The touchdown method was then applied to each primer, varying the number of final cycles (10 or 12) and the decrease in temperature (0.5 degrees or 1.0 degrees C intervals). The gels were compared, considering the revelation quality, band sharpness and the number of bands visualized. The touchdown-PCR method was more efficient for band amplification for most of the primers, especially at higher annealing temperatures. This type of system is useful for reducing the resources, time and effort needed for optimizing temperature conditions for a group of representative primers.

Improvement of a popcorn population using selection indexes from a fourth cycle of recurrent selection program carried out in two different environments.

We estimated genetic gains for popcorn varieties using selection indexes in a fourth cycle of intrapopulation recurrent selection developed in the campus of the Universidade Estadual do Norte Fluminense. Two hundred full-sib families were obtained from the popcorn population UNB-2U of the third recurrent selection cycle. The progenies were evaluated in a randomized block design with two replications at sites in two different environments: the Colégio Estadual Agrícola Antônio Sarlo, in Campos dos Goytacazes, and the Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (PESAGRO-RIO), in Itaocara, both in the State of Rio de Janeiro. There were significant differences between families within sets in all traits, indicating genetic variability that could be exploited in future cycles. Thirty full-sib families were selected to continue the program. The selection indexes used to predict the gains were those of Mulamba and Mock, Smith and Hazel. The best results were obtained with the Mulamba and Mock index, which allowed the prediction of negative gains for the traits number of diseased ears and ears attacked by pests, number of broken plants and lodging, as well as ears with poor husk cover. It also provided higher gains for popping expansion and grain yield than with the other indexes, giving values of 10.55 and 8.50%, respectively, based on tentatively assigned random weights.