The distribution of different virulence grass carp reovirus strains in some neglected tissues.

Grass carp reovirus (GCRV) is the causative agent of hemorrhagic disease in infected grass carp. During an outbreak, a mortality rate of up to 85% can be experienced, thus leading to substantial economic losses. The current understanding of disease pathogenesis is limited, with the distribution and dynamics of replication amongst different GCRV strains in vivo largely unknown. We determined distribution of different GCRV strains in infected grass carp, especially in some neglected tissues, such as the gill, brain, blood and so on. The results showed elevated viral RNA copy numbers in the blood, with some tissues such as the kidney, heart, brain, and bladder exhibiting even higher viral loads following infection with the virulent GCRV-CL strain. Even more interesting is that the brain exhibited the highest viral load, with a copy number of 800,000 following GCRV-CL infection. Overall, this study provides further insight into GCRV viral load distributions following infection and potentially identified some new viral tropism sites to provide a foundation for further studies aimed at characterizing GCRV viral pathogenesis.

N-doped SiO2/TiO2 mesoporous nanoparticles with enhanced photocatalytic activity under visible-light irradiation.

Mesoporous nanocrystalline N-doped SiO2/TiO2 visible-light photocatalysts were prepared by treating SiO2/TiO2 xerogels in a flow of nitrogen gas bubbled through concentrated ammonia solution. Structural characterization and performance analysis results revealed that the addition of SiO2 remarkably altered the phase composition, specific surface area, microstructure, as well as the photocatalytic activity of N-doped TiO2. The presence of SiO2 in N-doped TiO2 particles suppressed the formation of rutile phase and the crystal growth of N-doped TiO2 particles during thermal calcinations. When weight ratio of SiO2/TiO2 was in 0.05-0.20, the N-doped SiO2/TiO2 exhibited higher photocatalytic activity than the N-doped TiO2, and optimum ratio was found to be 0.05. The enhanced photocatalytic activity could be attributed to the higher specific area, larger pore volume, and more surface hydroxyl groups in the catalyst.

A study on the ecological habit of Armigeres subalbatus in Dawa area of the Mengshan Mountain in Shandong Province.

Armigeres subalbatus (A.s.) was reported in Shandong Province for the first time in 1965 and found in five counties of south Shandong including Pingyi, Linyi etc. in 1986. In Dawa area of the Mengshan mountain A.s. alults could be found in the first ten days of May, which increased in number in July, and become the dominant species in mosquito colonies in Aug. and Sept., then decreased gradually in number in Oct. and disappeared in Nov. There were two peaks of activity and blood-sucking behavior during the 24 hours of a day, one at dusk and the other at dawn. When the temperature dropped to 16 degrees C and below in the last ten days of Oct., the wigglers began their diapause period. The survival ratio reached 90.5% after 12 h freezing at -5 degrees C and none survived after 60 hours freezing. When the temperature rose to 17 degrees C and above the over-winterting larvae developed into adults, which could suck blood only at the temperature above 17.5 degrees C.

[Experimental safety study using rats and mice irradiated with the complex field cure instrument].

The complex field cure instrument is a new medical instrument. The complex field is composed of several sorts of physical quantum fields. Mice and rats are continuously irradiated by the complex field for 20 days to 90 days. The result shows that WBC, Hb and PLT value of the animals irradiated with the instrument are more than the control group, and their biochemical index is normal. The internal organs of the experimental animals show no toxicity. The visceval coefficient (liver, spleen) of the experimental animals is more than the control group. The marrow micronucleas test is negative. The chromosome test show no distortion. All of the results have proved that the instrument produces no toxicity side effect. Application of the instrument is safe and reliable and it may increase immune function of the body.

A rule-based approach for RNA pseudoknot prediction.

RNA plays a critical role in mediating every step of cellular information transfer from genes to functional proteins. Pseudoknots are functionally important and widely occurring structural motifs found in all types of RNA. Therefore predicting their structures is an important problem. In this paper, we present a new RNA pseudoknot structure prediction method based on term rewriting. The method is implemented using the Mfold RNA/DNA folding package and the term rewriting language Maude. In our method, RNA structures are treated as terms and rules are discovered for predicting pseudoknots. Our method was tested on 211 pseudoknots in PseudoBase and achieves an average accuracy of 74.085% compared to the experimentally determined structure. In fact, most pseudoknots discovered by our method achieve an accuracy of above 90%. These results indicate that term rewriting has a broad potential in RNA applications ranging from prediction of pseudoknots to discovery of higher level RNA structures involving complex RNA tertiary interactions.