Translation and validation of 17-item Wound-QoL questionnaire in a Chinese population.

We aimed to translate the 17-item questionnaire to measure the quality of life of patients with chronic wounds (Wound-QoL-17) and verify its reliability and validity in the Chinese population. The standard Chinese version of the Wound-QoL-17 was determined through translation, back translation, and cultural adaptation. A total of 121 patients with chronic wounds from the wound center of a tertiary hospital in Beijing were recruited. Through a questionnaire and physical examination, we tested the criterion-related validity, known group validity, structural validity, internal consistency coefficient (Cronbach's alpha), and test-retest correlation. A new structure of four factors was extracted by exploratory factor analysis, and the cumulative contribution rate was 72.23%. The total score and that of the four factors, which were significantly correlated with the EuroQol Five Dimensions Questionnaire (EQ-5D) and the Short Form-36 Health Survey (SF-36) (P < 0.05), also showed statistically significant differences between patients with different pain grades, with or without wound odour, and between different groups of patients reporting wound changes in the past 2 weeks. Cronbach's alpha was between 0.779 and 0.906, while the test-retest reliability was between 0.532 and 0.802. We concluded that the Chinese Wound-QoL-17 has good reliability and validity and is suitable for evaluating the quality of life of patients with chronic wounds.

Does Social Support Moderate Wound Pain and Health-Related Quality of Life in Patients With Chronic Wounds? A Multicenter Descriptive Cross-sectional Study.

PURPOSE: We sought to explore the relationships among social support, health-related quality of life (HRQOL), and wound pain, and to examine whether social support would moderate the relationship between wound pain and HRQOL. DESIGN: A multicenter descriptive cross-sectional study. SUBJECTS AND SETTINGS: Individuals with chronic wounds attending wound clinics affiliated with 3 public hospitals in Beijing, China. METHODS: Sociodemographic and wound characteristics of 162 participants were retrieved from medical records. Participants completed questionnaires for wound-related pain intensity (Numerical Rating Scale), social support (Social Support Rating Scale), and HRQOL (Medical Outcome Study Short Form-36). The moderating effect analysis was examined using the PROCESS analytic tool developed by Hayes, based on the bias-corrected bootstrapping method. RESULTS: Results revealed that higher pain intensity was significantly related to lower HRQOL (P < .01), and higher social support was associated with better HRQOL (P < .01). However, there was no significant correlation between social support and wound pain (P = .55). Importantly, the moderating effect of social support on the relationship between wound pain and HRQOL was statistically significant (P = .008). CONCLUSIONS: We found that social support moderated the impact of wound pain on HRQOL in patients with chronic wounds. This finding suggests that support obtained from social networks may be a beneficial intervention to improve the HRQOL of patients with chronic wounds, especially those suffering from high-intensity wound pain.

A Numerical Study of Micro-Droplet Spreading Behaviors on Wettability-Confined Tracks Using a Three-Dimensional Phase-Field Lattice Boltzmann Model.

Wettability-confined tracks have been extensively used in open-surface microfluidic devices for their high capacity of transporting droplet pumplessly. Inspired by the experimental work of Sen et al. [ Langmuir 2018 , 34 , 1899 - 1907 ], in the present study, a three-dimensional phase-field lattice Boltzmann model is developed and used to investigate the spreading behaviors of microdroplet on a series of wettability-confined tracks. The experimental findings are successfully reproduced through our simulation, where three distinct stages of droplet spreading on the horizontal wettability-confined diverging track are fairly exhibited, that is, the initial stage with droplet front spreading quickly, the intermediate stage with both droplet front and bulge moving forward at a constant speed, and the final stage with droplet front decelerating gradually. Moreover, a parametric study of track divergence angle is further performed, and the influential mechanism of track divergence angle on droplet spreading is further revealed. It is demonstrated that track divergence is responsible for the Laplace pressure gradient and capillary force inside the droplet, which drives the droplet bulge to move forward on the diverging track. With an increase in divergence angle, the capillary force increases linearly, which increases the droplet spreading speed at the initial and intermediate stages, while the peak capillary force comes earlier, and consequently the final decelerating stage comes earlier. On the basis of the parametric study and droplet volume conservation rule, a power law relation between track divergence angle and droplet spreading is proposed, which helps to identify the start of final decelerating stage. Finally, the droplet spreading over various inclined tracks is explored, which can be achieved only when the capillary force at the beginning is larger than the droplet gravity component along the inclined track surface.

Prevalence of myopia and vision impairment in school students in Eastern China.

BACKGROUND: Prevention of myopia has become a public health priority in China. This study is to investigate the prevalence of myopia and vision impairment, and their associated factors in school students in eastern China. METHOD: In this cross-sectional school-based study of 4801 students from 16 schools ranging from kindergarten to high school, students underwent refraction using non-cycloplegic autorefractor and visual acuity testing using logMAR chart with tumbling E. Myopia was defined as spherical equivalent (SPHE) ≤ - 0.5 diopter (D) and uncorrected visual acuity (UCVA) 20/25 or worse. High myopia was defined as SPHE ≤ - 6.0 D and UCVA 20/25 or worse. Vision impairment was defined as UCVA 20/40 or worse. Logistic regression models were used to determine factors associated with myopia and vision impairment. RESULTS: Among 4801 children (55% male) with mean age (standard deviation) 12.3 (3.8) years, 3030 (63.1, 95% CI: 61.7-64.5%) had myopia, 452 (9.4, 95% CI: 8.6-10.3%) had high myopia, and 2644 (55.1, 95% CI, 53.7-56.5%) had vision impairment. The prevalence rate of myopia increased with grade in a non-linear manner, 12% in kindergarten, 32% in grade 2, 69% in grade 5, and approximately 90% by grade 10 or above. The prevalence rate of high myopia was relatively low in grade 4 or below (< 1.5%), 4-7% in grade 5 to 7, 13-15% in grade 8-9, and > 20% in grade 10 to 12. The prevalence rate of vision impairment was 4% in kindergarten, 37% in elementary school, 77% in middle school and 87% in high school students. Higher grade (p < 0.0001), female (p < 0.0001) and higher school workload (p = 0.007) were independently associated with higher prevalence rates of myopia and vision impairment, while higher grade (p < 0.0001) and higher school workload (p < 0.0001) were independently associated with higher prevalence of high myopia. CONCLUSION: Prevalence of myopia and vision impairment was high among Chinese school students and increased with grade in a non-linear manner, reaching alarming high in high school students accompanied by high prevalence of high myopia. Increasing study burden on school students at younger age plays an important role on the higher prevalence rate of myopia and vision impairment.

Astigmatism in school students of eastern China: prevalence, type, severity and associated risk factors.

BACKGROUND: China has been undergoing dramatic economic development, accompanied by increased education load on the young children. This study is to investigate the prevalence, type, severity, and associated risk factors of astigmatism in school students in eastern China. METHOD: In this cross-sectional school-based study, students underwent refraction using NIDEK non-cycloplegic autorefractor. Astigmatism was defined as cylinder 1.5 diopter (D) or greater, and high astigmatism was defined as cylinder 3.0 D or greaterMultivariate regression models were used to determine factors associated with astigmatism. RESULTS: Among 4801 children (55% male) with mean age (±standard deviation) 12.3 (±3.8) years, 680 (14.2, 95% confidence interval (95% CI): 13.2-15.2%) had astigmatism (85% were with-the-rule) and 103 (2.2, 95% CI: 1.8-2.6%) had high astigmatism. The prevalence rate of astigmatism was 7-8% in grades 4 or below, 15-16% in grades 6-8, 20% in grade 9, and 20-25% in grade 10 or above. In multivariate analyses, higher grade and male gender were associated with higher prevalence of astigmatism (all p < 0.0001) and high astigmatism (p = 0.04 for grade, p = 0.001 for gender). When multivariate models were further adjusted by spherical equivalent, only gender remained statistically associated with astigmatism (odds ratio (OR) = 1.65, p < 0.0001) and high astigmatism (OR = 2.21, p = 0.0004), myopic and hyperopic refractive error were significantly associated with higher risk of astigmatism and high astigmatism (all p < 0.0001). CONCLUSION: Astigmatism is common in Chinese school-age children and increases with grade. Majority of astigmatism is with-the-rule. Male gender and myopic or hyperopic refractive error are significantly associated with higher prevalence and severity of astigmatism.

A numerical study of droplet dynamic behaviors on a micro-structured surface using a three dimensional color-gradient lattice Boltzmann model.

Inspired by the experimental work of Raj et al. (high-resolution liquid patterns via three-dimensional droplet shape control), in the present study, a three-dimensional multiphase color-gradient lattice Boltzmann model developed previously by some of the authors is used to simulate droplet dynamic behaviors with different surface micro-pillar arrays. To facilitate the present simulation, wetting boundary conditions are used and the accuracy of color gradient prediction at boundary nodes is enhanced. The experimental findings are confirmed and non-circular contact lines are reproduced numerically for the first time. To justify the existing contact angle formula proposed based on the Wenzel model, a systematic parametric study is conducted, based on which the pillar density is redefined to allow for the influence of pillar height, and then it is used to modify the contact angle. In addition, the evolution of the contact line motion for various droplet shapes is investigated systematically, and both circular and non-circular contact characteristics are well-depicted for different surface micro-pillar arrays.

[Gene construction and screening of humanized single chain antibody library against VSTM1-v2 cytokine].

To rapidly select potent anti-VSTM1-v2 scFv (single-chain antibody fragment) by construction and screening of a humanized scFv library in which a murine VH-CDR3 library was grafted onto a human scFv framework. A murine VH-CDR3 library was amplified from anti-VSTM1-v2 murine cDNA and grafted on human scFv (VH3-VK1) framework. Anti-VSTM1-v2 scFv templates were selected and enriched through ribosome display, TA-cloned into expression vector, and transformed into BL21 (DE3) for soluble expression of target scFv. A total of 1000 clones were randomly picked. Positive ones were first identified using colony PCR, indirect ELISA, Western blotting and then verified with sequencing and dose response ELISA. At last an anti-VSTM1-v2 humanized scFv with good binding affinity (EC50 = 21.35 nmol x L(-1)) was selected from the humanized library of 10(12) members generated in this study. This scFv antibody might have potential applications. This study provides a new approach for rapid screening of humanized antibodies.

Prevalence of the metabolic syndrome in Pudong New Area of Shanghai using three proposed definitions among Chinese adults.

BACKGROUND: The prevalence of metabolic syndrome (MS) has been increasing in China in recent years. The aim of this study is to estimate and compare the prevalence of MS among Chinese adults in Shanghai, one of the most economic developed areas in China, using definitions proposed by World Health Organization (WHO), National Cholesterol Education Program Adult Treatment Panel (modified ATP III) and International Diabetes Federation (IDF). METHODS: This cross-sectional study included 5,584 adults at age 20-79 randomly selected from Pudong New Area of Shanghai, China, through a three-stage sampling. All participants were interviewed in-person between April and July of 2008 to collect information on demographic and lifestyle characteristics. At the interview, anthropometry and blood pressure were measured and bio-specimens were collected. RESULTS: The prevalence estimates for the MS increased with age for each definition in men and women, but the estimates varied greatly between the definitions and by sex. The prevalence of the MS was higher in men (20.2%) than in women (18.7%) using WHO definition but this sex difference was reversed when using the modified ATP III (28.4% for men vs. 35.1% for women) and the IDF (15.9% for men vs. 26.7% for women) criteria. The most common metabolic disorder in this population was dyslipidaemia, regardless of the definition used. Substantial agreement, estimated using the kappa statistic, was found between the modified ATP III and IDF definition, whereas the lowest agreement was observed between the WHO and ATP III criteria. CONCLUSIONS: The MS is highly prevalent among Chinese adults in Pudong New Area of Shanghai and the most prevalent component was dyslipidemia. These findings underscore the importance of prevention and control efforts for the MS in this area and the need for a unified predictive definition for the syndrome for use by clinical practitioners and public health agencies.

Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.

MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO). Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1. The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail. The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C. The rate-determining step was the boundary diffusion and intra-particle diffusion. The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.

N-Doped Carbon-Coated ZnS with Sulfur-Vacancy Defect for Enhanced Photocatalytic Activity in the Visible Light Region.

In this work, N-doped carbon-coated ZnS with a sulfur-vacancy defect (ZnS@N-C) was performed for the visible-light-driven photodegradation of tetracycline hydrochloride (TCH). The obtained ZnS@N-C exhibited enhanced photocatalytic activity compared with ZnS for TCH removal. Among these ZnS@N-C composites, ZnS@N-C-3 with N-doped content of 3.01% (100 nm) presented the best visible-light photocatalytic activity and superior long-term photocatalytic stability after five cycle times for TCH removal in the visible light region. This may be ascribed to the interface between the N-doped carbon shell and ZnS with a sulfur-vacancy defect for efficient charge transfer and the restrained recombination of charge carriers. Electron spin resonance (ESR) results indicate that the ·O2‒ radical plays a crucial role in the enhanced photocatalytic activity of ZnS@N-C-3.

CuInS2/Mg(OH)2 Nanosheets for the Enhanced Visible-Light Photocatalytic Degradation of Tetracycline.

CuInS2/Mg(OH)2 (CIS/Mg(OH)2) nanosheets have been prepared for the visible light activated photodegradation of tetracycline hydrochloride (TCH). The introduction of CuInS2 has proven to enhance the photocatalytic activity of Mg(OH)2 nanosheets. It's ascribed to the enhanced transfer and separation of charge carriers at the junction interface between CuInS2 and Mg(OH)2. The photocatalytic activity of obtained CIS/Mg(OH)2 is greatly affected by CuInS2 content, pH value, and inorganic ions. Among these samples, 2-CIS/Mg(OH)2 exhibits the excellent photocatalytic activity and durability for the visible light driven removal of TCH after five cycle times. Atomic force microscope (AFM) images indicate that the surface roughness of 2-CIS/Mg(OH)2 is intensively influenced in adsorption-photocatalysis process. The •O2- and •OH radicals are vital for the visible light driven photocatalytic activity of 2-CIS/Mg(OH)2 for TCH removal.

Enhanced Visible-Light Photocatalytic Activity of Ag QDs Anchored on CeO2 Nanosheets with a Carbon Coating.

Ag quantum dots (QDs) anchored on CeO2 nanosheets with a carbon coating (Ag/CeO2@C) (composites) were prepared via an in situ reduction approach for the photocatalytic degradation of Cr(VI) and tetracycline hydrochloride (TCH) in the visible-light region. The photocatalytic activity of Ag/CeO2@C was greatly affected by carbon content, Ag-doping content, Cr(VI) concentration, pH value, and inorganic ions. Enhanced photocatalytic activity was obtained by Ag/CeO2@C (compared to CeO2 and CeO2@C), of which 3-Ag/CeO2@C-2 with an Ag-doping content of 5.41% presented the best removal efficiency and the most superior stability after five cycles. ·O2- and ·OH radicals were crucial for the photocatalytic capacity of 3-Ag/CeO2@C-2. The combined effect of the surface plasma resonance (SPR) of Ag QDs, an electron trapper of carbon shells, and the redox activity of the Ce(III)/Ce(IV) coupling induced efficient charge transfer and separation, suppressing the recombination of electron-hole pairs.

Comparison of fibrin sealant and sutures for conjunctival autograft fixation in pterygium surgery: one-year follow-up.

AIMS: To evaluate the efficacy and safety of fibrin sealant in conjunctival autograft fixation in pterygium surgery. METHODS: Prospective, comparative, interventional case series. 40 eyes of 40 patients with nasal primary pterygium, 24 male and 16 female, were enrolled. The patients were assigned to two groups and each contained 12 male and 8 female based on the pterygium area encroaching onto the cornea. In one group the conjunctival autograft was attached to the sclera with fibrin sealant and the other group with nylon 10-0 sutures. All the patients were followed up postoperatively on days 1, 3, 7 and 14 then at months 1, 2, 6 and 12. The main outcome measures included operating time, postoperative symptoms, graft success, recurrence rate and complications. RESULTS: The average operating time was significantly shorter (p < 0.001) in the fibrin sealant group and fewer postoperative symptom complaints were received as well. By the end of 1-year follow-up, the recurrence rate was 5% in the fibrin sealant group and 10% in the suture group (p = 1.000), and there was no statistically significant difference in complications (p > 0.05) between the two groups. CONCLUSIONS: It is safe and effective to use fibrin sealant for conjunctival autograft fixation. This method causes much fewer postoperative symptoms and shortens surgery time significantly, and the long-term results are also favorable.