Influence of TNF-α-308 G/A gene polymorphism on temporomandibular disorder.

INTRODUCTION: Tumor necrosis factor alpha (TNF-α) levels are significantly upregulated in the synovial fluid of patients with temporomandibular joint disorder (TMD). The TNF-α influences pain generation and maintenance. Therefore, the aim of this study was to investigate the influence of single nucleotide polymorphism TNFA-308 (rs1800629) on TMD risk and on the pressure pain threshold. METHODS: The genotypic and allelic frequencies of candidate single nucleotide polymorphisms were compared among 152 TMD patients and 91 sex- and age-matched healthy subjects in the control group using the real-time polymerase chain reaction technique. The pressure pain threshold in the temporomandibular joint, anterior fascicle of the temporal muscle, masseter muscle, and Achilles tendon were recorded with an algometer. After the pressure test, all participants received a complete physical examination, including masticatory muscle evaluation, temporomandibular joint palpation, and assessment of mandibular range of motion. RESULTS: The TNFA-308 polymorphism is positively associated with TMD. Subjects with TMD had a 2.87 (95% confidence interval, 1.256-6.569) times greater chance of having the GA genotype than did the control group. Rare A-allele homozygotes demonstrated decreased pain sensitivity for the temporomandibular joint and anterior fascicle of the temporal muscle in the pressure pain threshold test compared with ancestral allele homozygotes. CONCLUSIONS: This study presents an unprecedented association between the TNFA-308 (rs1800629) polymorphism and TMD. Future studies are needed to enlighten the association between TNFA-308 G/A single nucleotide polymorphism and mechanical pain sensitivity.

Painful Temporomandibular Joint Clicking: Genetic Point of View.

AIMS: To determine whether there is an association between gene polymorphisms and patients with painful temporomandibular joint (TMJ) clicking when compared to patients with painless TMJ clicking and a healthy control group. METHODS: In this pilot study, the genotypic and allelic frequencies of candidate single-nucleotide polymorphisms (SNP) were compared among 60 individuals divided equally into three groups: patients with painful TMJ clicking (n = 20); patients with painless TMJ clicking (n = 20); and healthy controls (n = 20). Participants were genotyped for the following SNPs using real-time polymerase chain reaction: MMP1 -16071G/2G, COMT Val158Met, TNFα -308, IL1ß +3954, IL6 -174, and IL10 -1082. The pressure pain threshold (PPT) of the TMJ was also assessed. All variables were compared among groups. RESULTS: Patients with painful TMJ clicking had a significant association and a higher frequency of MMP1 -16071G/2G (P = .042), COMT Val158Met (P = .030), and TNFα -308 (P = .016) when compared to the other groups, as well as a lower frequency of IL10 -1082. Considering PPT values, a progressively lower mean was found in individuals with painful TMJ clicking, followed sequentially by the painless TMJ clicking and the control groups. CONCLUSION: This pilot study showed that patients with painful TMJ clicking had a significant association with mutant genotypes related to degradation of extracellular matrix components, pain, proinflammation, and anti-inflammation. Furthermore, these patients also had significantly lower TMJ PPT values in all comparisons.

Insights for temporomandibular disorders management: From psychosocial factors to genetics-A case report.

AIMS: This case report aimed to discuss the multifactorial etiology and also the management of temporomandibular disorders (TMD) by addressing important associated psychosocial and biological factors, emphasizing the interaction between these factors and a probable genetic predisposition. METHODS AND RESULTS: A 21-year-old female patient was evaluated according to Research Diagnostic Criteria for TMD and diagnosed with arthralgia, myofascial pain, disc displacement without reduction, and temporomandibular joint (TMJ) degenerative disease. TMJ alterations were confirmed through magnetic resonance imaging and cone-beam computed tomography. Pressure pain threshold of masticatory structures was evaluated using a pressure algometer. Sleep bruxism, poor sleep quality, migraine with aura, mild anxiety, and history of facial trauma were also identified through anamnesis and clinical examination. Following this, genetic analysis was performed to evaluate the presence of single nucleotide polymorphisms (SNPs) already associated with TMD: SNP COMT Val158 Met (rs4680), MMP1-1607 (rs1799750), and tumor necrosis factor alpha-308 (rs1800629), which were all present. A personalized treatment for TMD management was performed, and it included self-management programs, occlusal appliance therapy, pharmacotherapy, anxiety management, and stress control. An 8-year follow-up demonstrated long-term stabilization of TMJ degenerative disease. CONCLUSION: Genetic evaluation, added to anamnesis and clinical examination, could be useful for TMD prognosis and management.

Role of inflammatory and pain genes polymorphisms in temporomandibular disorder and pressure pain sensitivity.

OBJECTIVE: The aim of this study was to assess the correlation of inflammatory and pain genes polymorphisms with the presence of temporomandibular disorder (TMD) patients and with pressure pain sensitivity. DESIGN: Data was collected from 268 consecutive subjects at Bauru School of Dentistry. Subjects aged younger than 20 years, with dental and neuropathic pain, sinusitis, cognitive and neurologic disorder were excluded. Included subjects were evaluated using the Research Diagnostic Criteria for Temporomandibular disorders and divided into two groups: TMD cases and healthy controls. Groups were submitted to pressure pain threshold (PPT) test for the temporomandibular joint, anterior temporalis and masseter muscles and genotyped for Val158Met, IL6-174, IL-1ß-3954 and TNFA-308. Student's t-test and Pearson chi-square test were used to comparisons between groups. A linear multiple regression was used to evaluate the influence of genetics variables on the PPT and a bivariate analysis was used to assesses the influence of genetics variables on pain sensitivity below the PPT cut off of the structures in TMD group. RESULTS: TMD group showed significantly lower PPT values for all structures when compared with control group (p < 0.001). SNP IL6-174 predicted higher pain sensitivity in the temporomandibular joint (p < 0.005) and in anterior temporalis muscle (p < 0.044) and SNP Val158Met in the masseter muscle (p < 0.038); when TMD group was divided according to PPT cut-off values the SNP Val158Met influenced increase pain sensibility in the masseter muscle. CONCLUSION: TNFA-308 was associated with TMD and SNP IL6-174 and SNP Val158Met influenced pain sensitivity of patients with TMD.

The dilemma of the open gingival embrasure between maxillary central incisors.

AIM: The aim of this report is to present the etiology, diagnosis, and treatment planning strategy in the presence of an open gingival embrasure between the maxillary central incisors. BACKGROUND: The open gingival embrasure or "black triangle" is a visible triangular space in the cervical region of the maxillary incisors. It appears when the gingival papilla does not completely fill in the interdental space. The space may occur due to: (1) disease or surgery with periodontal attachment loss resulting in gingival recession; (2) severely malaligned maxillary incisors; (3) divergent roots; or (4) triangular-shaped crowns associated with or without periodontal problems and alveolar bone resorptions. REPORT: The post-treatment prevalence in adult orthodontic patients is estimated to be around 40% compromising the esthetic result. CONCLUSION: Several methods of managing patients with open gingival embrasure exist, but the interdisciplinary aspects of treatment must be emphasized to achieve the best possible result. The orthodontist can play a significant role in helping to manage these cases. CLINICAL SIGNIFICANCE: Various treatment strategies are available to treat cases of an undesirable black triangle and are dependent on the etiology of the condition.

Comparative effects of the Mandibular Protraction Appliance in adolescents and adults.

OBJECTIVE: The aim of this study was to compare the skeletal, dental, and soft tissue effects of the Mandibular Protraction Appliance (MPA) application in adolescent and adult Class II malocclusion patients. METHODS: The sample comprised the pretreatment and posttreatment lateral cephalograms of 39 subjects presenting Class II malocclusion treated with the MPA and fixed appliances. Sample was divided into two groups: Group 1 comprised 23 subjects (10 male; 13 female), at a mean pretreatment age of 11.75 years, with a mean treatment time of 3.32 years; Group 2 included 16 subjects (7 male; 9 female), at a mean pretreatment age of 22.41 years, with a mean treatment time of 4.24 years. Intergroup comparison of the initial and final stages and treatment changes between the groups was performed with t tests, at p< 0.05. RESULTS: The adults showed less significant amounts of skeletal, dentoalveolar and soft tissue changes than the adolescents. There was significantly greater palatal tipping of the maxillary incisors and retrusion of the upper lip in the adolescents. The adult group showed greater mandibular incisor proclination in the posttreatment stage. CONCLUSION: Adult patients treated with MPA showed less significant amounts of skeletal, dentoalveolar and soft tissue changes than adolescents.

TMD and chronic pain: a current view.

This review aims at presenting a current view on the physiopathologic mechanisms associated with temporomandibular disorders (TMDs). While joint pain is characterized by a well-defined inflammatory process mediated by tumor necrosis factor-α and interleukin, chronic muscle pain presents with enigmatic physiopathologic mechanisms, being considered a functional pain syndrome similar to fibromyalgia, irritable bowel syndrome, interstitial cystitis and chronic fatigue syndrome. Central sensitization is the common factor unifying these conditions, and may be influenced by the autonomic nervous system and genetic polymorphisms. Thus, TMDs symptoms should be understood as a complex response which might get worse or improve depending on an individual's adaptation.

Effects of mandibular protraction appliance associated to fixed appliance in adults.

OBJECTIVE: This retrospective study aimed to conduct a cephalometric evaluation of the skeletal, dental and soft tissue effects resulting from treatment of adult patients presenting Class II malocclusion, performed with a Mandibular Protraction Appliance (MPA) combined with a fixed orthodontic appliance. METHODS: The sample consisted of teleradiographs obtained before and after treatment of 9 adult patients (initial mean age of 22.48 years) with bilateral Class II, division 1, malocclusion. Paired t test (p < 0.05) was applied to compare initial and final values. RESULTS: t test revealed an increase in anteroinferior facial height and posterior facial height. The dental changes include: extrusion of upper incisors, buccal inclination, protrusion of lower incisors, mesialization and extrusion of mandibular molars. Regarding the soft tissue component, there was an increase in nasolabial angle in addition to upper lip retrusion. CONCLUSIONS: The effects of treating Class II malocclusion adult patients, by means of using Mandibular Protraction Appliance (MPA) combined with a fixed appliance were mostly observed in the mandibular arch, and consisted of buccal inclination, protrusion and intrusion of incisors, and mesialization and extrusion of the molars.

Comparative effects of the Mandibular Protraction Appliance in adolescents and adults

ABSTRACT Objective: The aim of this study was to compare the skeletal, dental, and soft tissue effects of the Mandibular Protraction Appliance (MPA) application in adolescent and adult Class II malocclusion patients. Methods: The sample comprised the pretreatment and posttreatment lateral cephalograms of 39 subjects presenting Class II malocclusion treated with the MPA and fixed appliances. Sample was divided into two groups: Group 1 comprised 23 subjects (10 male; 13 female), at a mean pretreatment age of 11.75 years, with a mean treatment time of 3.32 years; Group 2 included 16 subjects (7 male; 9 female), at a mean pretreatment age of 22.41 years, with a mean treatment time of 4.24 years. Intergroup comparison of the initial and final stages and treatment changes between the groups was performed with t tests, at p< 0.05. Results: The adults showed less significant amounts of skeletal, dentoalveolar and soft tissue changes than the adolescents. There was significantly greater palatal tipping of the maxillary incisors and retrusion of the upper lip in the adolescents. The adult group showed greater mandibular incisor proclination in the posttreatment stage. Conclusion: Adult patients treated with MPA showed less significant amounts of skeletal, dentoalveolar and soft tissue changes than adolescents.

RESUMO Objetivo: o objetivo do presente estudo foi comparar os efeitos esqueléticos, dentários e em tecidos moles do Aparelho de Protração Mandibular (APM) em pacientes adolescentes e adultos com má oclusão de Classe II. Métodos: a amostra consistiu das telerradiografias pré- e pós-tratamento de 39 indivíduos com má oclusão de Classe II tratados com APM e aparelhos fixos. A amostra foi dividida em dois grupos: o Grupo 1 compreendeu 23 indivíduos (10 homens e 13 mulheres), com idade inicial de 11,75 anos, com um tempo médio de tratamento de 3,32 anos; o Grupo 2 incluiu 16 pacientes (7 do sexo masculino e 9 do sexo feminino), com idade média inicial de 22,41 anos, com um tempo médio de tratamento de 4,24 anos. A comparação intergrupos dos estágios inicial e final e das alterações com o tratamento foi realizada com testes t, considerando-se um nível de significância de p< 0,05. Resultados: os adultos apresentaram quantidades menos significativas de alterações esqueléticas, dentoalveolares e de tecidos moles do que os adolescentes. Houve uma inclinação palatina significativamente maior dos incisivos superiores e retrusão do lábio superior nos adolescentes. O grupo de adultos apresentou maior proclinação dos incisivos inferiores no estágio pós-tratamento. Conclusão: pacientes adultos tratados com APM apresentaram quantidades menos significativas de alterações esqueléticas, dentoalveolares e de tecidos moles do que os adolescentes.

Maxillary protraction after surgically assisted maxillary expansion.

This case report describes the orthodontic treatment of a 32-year-old woman with a Class III malocclusion, whose chief compliant was her dentofacial esthetics. The pretreatment lateral cephalometric tracings showed the presence of a Class III dentoskeletal malocclusion with components of maxillary deficiency. After discussion with the patient, the treatment option included surgically assisted rapid maxillary expansion (SARME) followed by orthopedic protraction (Sky Hook) and Class III elastics. Patient compliance was excellent and satisfactory dentofacial esthetics was achieved after treatment completion.

TMD and chronic pain: A current view

This review aims at presenting a current view on the physiopathologic mechanisms associated with temporomandibular disorders (TMDs). While joint pain is characterized by a well-defined inflammatory process mediated by tumor necrosis factor-α and interleukin, chronic muscle pain presents with enigmatic physiopathologic mechanisms, being considered a functional pain syndrome similar to fibromyalgia, irritable bowel syndrome, interstitial cystitis and chronic fatigue syndrome. Central sensitization is the common factor unifying these conditions, and may be influenced by the autonomic nervous system and genetic polymorphisms. Thus, TMDs symptoms should be understood as a complex response which might get worse or improve depending on an individual's adaptation.

Esta revisão teve como objetivo apresentar uma visão atualizada dos mecanismos fisiopatológicos relacionados às disfunções temporomandibulares (DTMs). Enquanto a dor articular é caracterizada por um processo inflamatório bem descrito - mediado pelo fator de necrose tumoral alfa (TNF-α) e interleucinas -, a dor muscular crônica apresenta mecanismos fisiopatológicos mais obscuros, sendo considerada uma síndrome dolorosa funcional, assim como a fibromialgia, a síndrome do intestino irritável, a cistite intersticial e a síndrome da fadiga crônica. A sensibilização central é o processo comum, unificador, dessas condições, podendo sofrer influência do sistema nervoso autonômico e de polimorfismos genéticos. Portanto, os sintomas das DTMs devem ser entendidos como uma resposta complexa, podendo ser amplificados ou atenuados em função da adaptação individual.

Effects of mandibular protraction appliance associated to fixed appliance in adults

OBJECTIVE: This retrospective study aimed to conduct a cephalometric evaluation of the skeletal, dental and soft tissue effects resulting from treatment of adult patients presenting Class II malocclusion, performed with a Mandibular Protraction Appliance (MPA) combined with a fixed orthodontic appliance. METHODS: The sample consisted of teleradiographs obtained before and after treatment of 9 adult patients (initial mean age of 22.48 years) with bilateral Class II, division 1, malocclusion. Paired t test (p < 0.05) was applied to compare initial and final values. RESULTS: t test revealed an increase in anteroinferior facial height and posterior facial height. The dental changes include: extrusion of upper incisors, buccal inclination, protrusion of lower incisors, mesialization and extrusion of mandibular molars. Regarding the soft tissue component, there was an increase in nasolabial angle in addition to upper lip retrusion. CONCLUSIONS: The effects of treating Class II malocclusion adult patients, by means of using Mandibular Protraction Appliance (MPA) combined with a fixed appliance were mostly observed in the mandibular arch, and consisted of buccal inclination, protrusion and intrusion of incisors, and mesialization and extrusion of the molars.

OBJETIVO: esse estudo retrospectivo teve como objetivo avaliar, cefalometricamente, os efeitos esqueléticos, dentários e tegumentares decorrentes do tratamento com o Aparelho de Protração Mandibular em conjunto ao aparelho fixo em pacientes adultos para correção da má oclusão de Classe II. MÉTODOS: a amostra foi composta por telerradiografias pré- e pós-tratamento de nove adultos (idade inicial média de 22,48 anos), portadores de má oclusão de Classe II, divisão 1 bilateral. O teste t pareado (p < 0,05) foi empregado para comparação dos valores iniciais e finais. RESULTADOS: de acordo com o teste t, observou-se aumento da altura facial anteroinferior e da altura facial posterior. As alterações dentárias foram: extrusão dos incisivos superiores, inclinação para vestibular e protrusão dos incisivos inferiores; e mesialização e extrusão dos molares inferiores. Com relação ao componente tegumentar, houve aumento do ângulo nasolabial e retrusão do lábio superior. CONCLUSÃO: os efeitos do tratamento com o Aparelho de Protração Mandibular em conjunto com aparelho fixo em pacientes adultos para correção da má oclusão de Classe II foram direcionados, principalmente, à arcada inferior, com inclinação vestibular, protrusão e intrusão dos incisivos e mesialização e extrusão dos molares.

Maxillary protraction after surgically assisted maxillary expansion

This case report describes the orthodontic treatment of a 32-year-old woman with a Class III malocclusion, whose chief compliant was her dentofacial esthetics. The pretreatment lateral cephalometric tracings showed the presence of a Class III dentoskeletal malocclusion with components of maxillary deficiency. After discussion with the patient, the treatment option included surgically assisted rapid maxillary expansion (SARME) followed by orthopedic protraction (Sky Hook) and Class III elastics. Patient compliance was excellent and satisfactory dentofacial esthetics was achieved after treatment completion.

Potencial influência de variantes genéticas nas disfunções temporomandibulares / Potential influence of genetic variants in temporomandibular disorders

O presente estudo teve como objetivos testar a hipótese nula de que não há diferença entre o grupo com Disfunção Temporomandibular (DTM) e o grupo Controle, para as frequências genotípicas e de alelos dos SNPs candidatos COMT Val(158)Met (rs4680); TNFA-308 (rs1800629); IL6-174 (rs1800795); IL-1β-3954 (rs1143634); IL10-592 (rs1800872); MMP1-1607 (rs1799750) e TLR4-896 (rs4986790); e determinar, nos casos com DTM, a influência dos SNPs candidatos na sensibilidade dolorosa mecânica experimental, na intensidade e no tempo de dor. As frequências genotípicas e de alelos dos sete SNPs candidatos foram comparadas entre 152 indivíduos com DTM e 91 controles, por meio da técnica de PCR em tempo real. A sensibilidade dolorosa mecânica foi avaliada pelo limiar de dor à palpação (LDP) e a intensidade de dor, utilizando-se uma escala analógica visual. As diferenças entre as frequências dos polimorfismos estudados foram avaliadas por meio do teste qui-quadrado (x2) e os valores de LDP entre os grupos foram comparados pelo teste t. A análise de possíveis diferenças entre os subgrupos de genótipos, com relação aos valores de LDP, EAV e tempo de dor, foi feita pelo teste estatístico one-way ANOVA, seguido pelo teste de Tukey. A hipótese nula foi rejeitada, uma vez que houve diferença entre as frequências genotípicas do SNP TNFA-308 entre os grupos, estando o polimorfismo TNFA-308 (1800629) associado às DTMs (p<0,05). Além do TNFA-308, os SNPs COMT Val(158)Met, IL6-174, IL-1β-3954 e TLR4-896 foram capazes de influenciar a sensibilidade dolorosa mecânica em indivíduos doentes (p<0,05). O presente estudo apresenta associação inédita entre o polimorfismo TNFA-308 (1800629) e as DTMs. Encorajamos estudos futuros que elucidem a associação entre genótipos pró-inflamatórios e uma menor sensibilidade dolorosa mecânica.

This study aimed at testing the null hypothesis which claims that there is no difference between the group with temporomandibular joint disorder (TMD) and a control group regarding the genotypic and allelic frequencies of the candidate SNPs COMT Val(158)Met (rs4680); TNFA-308 (rs1800629); IL6-174 (rs1800795); IL-1β-3954 (rs1143634); IL10-592 (rs1800872); MMP1-1607 (rs1799750) and TLR4-896 (rs4986790). Additionally, it aimed at determining candidate SNPs influence on experimental mechanical pain sensitivity, intensity and duration of pain in the TMD cases. The genotypic and allelic frequencies of the seven candidate SNPs were compared among 152 individuals from the group with TMD and 91 individuals from the control group, by using the real-time PCR technique. Mechanical pain sensitivity was assessed through pressure pain threshold (PPT) and pain intensity was assessed using visual analogue scales (VAS). Differences between the frequencies regarding the studied polymorphisms were assessed through the chi-square test (x2) while the PPT values were compared through the t-test. Analysis of potential differences between the genotype subgroups with respect to the PPT, VAS and pain duration values was performed with ANOVA one-way test, followed by the Tukeys test. The null hypothesis was rejected, since there were differences among the genotypic frequencies of the TNFA-308 SNP between groups. The TNFA-308 (1800629) polymorphism is found to be associated with the TMDs (p<0.05). In addition to TNFA-308, the SNPs COMT Val(158)Met, IL6-174, IL-1β-3954 and TLR4-896 were capable of influencing mechanical pain sensitivity in individuals with TMD (p<0.05). This study presents an unprecedented association between the TNFA-308 (1800629) polymorphism and TMDs. We encourage future researches that enlighten the association between proinflammatory genotypes and lower mechanical pain sensitivity.

Relação entre DTM e tratamento ortodôntico na visão de clínicos gerais, especialistas em Ortodontia e especialistas em Disfunção Temporomandibular e Dor Orofacial / Relationship between TMD and orthodontic treatment in the opinion of dental general practitioners, specialists in Orthodontics and specialists in TMD and Orofacial Pain

Objetivo: o objetivo do presente estudo foi avaliar a visão de clínicos gerais (CG), especialistas em Ortodontia (EO) e especialistas em Disfunção Temporomandibular e Dor Orofacial (EDTM), por meio de questionário, quanto à inter-relação tratamento ortodôntico e DTM. Métodos: foi enviado questionário via correio eletrônico a 9.490 cirurgiões-dentistas de diferentes regiões do Brasil. Resultados: dos 132 profissionais que responderam à pesquisa, 75% relatou examinar, usualmente, seus pacientes quanto a sinais e sintomas de DTM previamente ao tratamento odontológico. A maioria dos profissionais (55%) afirmou acreditar que tratamento ortodôntico pode prevenir DTM; 68% dos EDTM não concordam com essa opinião. A maior parte (62%) associou tratamento ortodôntico à causa da DTM e não acredita que Ortodontia possa tratar a referida desordem (56%). A maioria dos CG (64%), no entanto, considera que se pode tratar DTM por meio de Ortodontia. Conclusão: a maioria dos profissionais acredita que tratamento ortodôntico pode prevenir DTM, mas que, ao mesmo tempo, não pode curar e pode ainda causar tal desordem. Foi encontrada diferença estatisticamente significativa em relação às respostas das três áreas de interesse (CG, EO e EDTM).

Tratamento ortodôntico de oclusões mutiladas / Orthodontics treatment of mutilated dentition

O presente artigo relata o tratamento ortodôntico de duas pacientes adultas portadoras de más oclusões com ausência múltipla de elementos dentários. Esses casos, em sua maior parte, demandam o concurso de outras especialidades, onde a reabilitação protética desempenha um papel essencial.