[Both alpha1- and beta-adrenergic receptors participate in generation of a Ca2+-response to noradrenaline in murine brown preadipocytes]. / V generatsii Ca2+-otveta na noradrenalin v burykh preadipotsitakh myshi uchastvuiut kak alpha1-, tak i beta-adrenoretseptory.

It has been shown that in Ca(2+)-signal generation, at the addition of norepinephrine (NE) to a suspension of freshly isolated brown preadipocytes, beta-receptors participate alongside with alpha 1-adrenoreceptors. The amplitude of cell response to 10(-5)-10(-8) M NE is approximately equal to the sum of effects of alpha 1-specific agonists (oxymetazoline or cirazoline) and beta-selective agonist isoproterenol. beta-Selective antagonist nadolol practically completely prevented the effect of NE on the intracellular Ca2+ level, while phentolamine, an antagonist of alpha-receptors, caused only a approximately 25% inhibition of the cellular response.