RESUMO
The therapeutic radiomitigating effect of cystamine and indralin was studied in experiments on mice and rats and pharmacological analysis of these drugs was carried out. The animals were subjected to whole-body 60Co γ-irradiation. The mice were exposed to single (9-10 Gy) or double (8 Gy) irradiation with an interval of 1 month. The rats were exposed to 10 Gy with partial shielding of the upper quarter of the abdomen. In experiments on mice, pretreatment with reserpine abolished the therapeutic effect of cystamine administered repeatedly every 15 min over 1 h after irradiation. Moreover, summation of the radioprotective and therapeutic effects of the radioprotector was revealed under these conditions. In mice and rats, α1-adrenoreceptor blocker terazosin did not abolish the therapeutic effect of indralin administrated after irradiation, but blocked the radioprotective effect of indralin applied prior to irradiation. At the same time, 5-HT2 serotonin receptor blocker tropoxin abolished the therapeutic effect of indralin without affecting its radioprotective activity.
Assuntos
Cistamina/farmacologia , Raios gama , Fenóis/farmacologia , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/farmacologia , Antagonistas de Receptores Adrenérgicos alfa 1/farmacologia , Animais , Compostos Aza/farmacologia , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Cistamina/antagonistas & inibidores , Relação Dose-Resposta à Radiação , Esquema de Medicação , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Fenóis/antagonistas & inibidores , Prazosina/análogos & derivados , Prazosina/farmacologia , Lesões Experimentais por Radiação/metabolismo , Lesões Experimentais por Radiação/patologia , Ratos , Receptores Adrenérgicos alfa 1/metabolismo , Receptores 5-HT2 de Serotonina/metabolismo , Reserpina/farmacologia , Irradiação Corporal TotalRESUMO
Female rats were exposed to local γ-irradiation of the right hindpaw in doses of 30-50 Gy at 131-154 sGy/min dose rate. Radioprotector indralin was administered per os 15 min prior to irradiation, monizol was injected intraperitoneally 5 min after irradiation. Indralin showed marked radioprotective properties both for acute and delayed symptoms of local radiation injuries. In combination with monizol, radioprotective effect of indralin was potentiated to dose reduction factor of 1.4-1.5 both for radiation burn severity reduction and for restriction of postradiational contracture development and amputation of the irradiated limb.
Assuntos
Raios gama , Dinitrato de Isossorbida/análogos & derivados , Fenóis/administração & dosagem , Lesões Experimentais por Radiação/tratamento farmacológico , Protetores contra Radiação/administração & dosagem , Dermatopatias/tratamento farmacológico , Doença Aguda , Administração Oral , Animais , Quimioterapia Combinada , Feminino , Injeções Intraperitoneais , Dinitrato de Isossorbida/administração & dosagem , Dinitrato de Isossorbida/farmacologia , Fenóis/farmacologia , Lesões Experimentais por Radiação/patologia , Protetores contra Radiação/farmacologia , Ratos , Pele/patologia , Pele/efeitos da radiação , Dermatopatias/etiologia , Resultado do TratamentoRESUMO
We studied the effect of long-term administration of melatonin to male C57Bl/6 mice starting from day 3 after whole-body γ-irradiation (9.5-10.0 Gy, 7.7-17.1 cGy/min). It was found that replacement of drinking water with melatonin solution (5 mg/liter) did not reduce the amount of fluid intake throughout the period of acute radiation injury. The daily dose of melatonin was 0.9-1.2 mg/kg body weight (this parameter was lower at the peak of the disease and increased during the recovery stage). Melatonin by more than 20% (p<0.05) improved survival of mice exposed to γ-irradiation in a dose of LD97/30, reduced leukopenia during the stage of acute manifestations of the disease and maximum mortality, and increased blood leukocyte count by 40% (p<0.05) by day 12 after irradiation.
Assuntos
Síndrome Aguda da Radiação/tratamento farmacológico , Antioxidantes/uso terapêutico , Melatonina/uso terapêutico , Protetores contra Radiação/uso terapêutico , Síndrome Aguda da Radiação/mortalidade , Animais , Raios gama/efeitos adversos , Contagem de Leucócitos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Irradiação Corporal Total/efeitos adversosRESUMO
On the basis of graphic models of the human response to environmental factors, two main types of complex quantitative influence as well as interrelation between determined effects at the level of an individual, and stochastic effects on population were revealed. Two main kinds of factors have been suggested to be distinguished. They are essential factors and accidental factors. The essential factors are common for environment. The accidental factors are foreign for environment. The above two kinds are different in approaches of hygienic standardization Accidental factors need a dot-like approach, whereas a two-level range approach is suitable for the essential factors.
Assuntos
Ecologia , Exposição Ambiental , Poluentes Ambientais/normas , Higiene/normas , Modelos Teóricos , Gráficos por Computador , Exposição Ambiental/análise , Exposição Ambiental/normas , Humanos , Concentração Máxima Permitida , Saúde Pública , Fatores de Risco , Federação Russa , Fatores SocioeconômicosRESUMO
The study of indralin radioprotective properties at its joint application with cystamine and mexamine was carried out in the experiments on inbred mice and rats. The mice and rats were exposed to whole-body y-irradiation at a dose of 9.0 and 9.5 Gy, correspondingly. A combined parenteral administration ofindralin and cystamine at a dose of 25 mg/kg showed ponentiaton of indralin radioprotective properties up to a level of the ED50 effect versus the absence of or a weak radioprotective effect in the case of their separate application. In the experiments on rats, indralin (50 mg/kg) and mexamine (12 mg/kg) injected intraperitoneally almost completely eliminated the animal mortality from the intestinal syndrome of acute radiation sickness amounting in the control radiation group to 60% on the 7th day after exposure to radiation at a dose of 9.5 Gy. However, at the above conditions, radioprotectors at these doses had a low-level radioprotective action at the onset of the bone marrow syndrome of acute radiation sickness. Combined application of indralin and mexamine at the same doses and at the same conditions led to a radiation protection 50% as high as in the case when radioprotectors were applied separately at a double dose.
Assuntos
5-Metoxitriptamina/uso terapêutico , Cistamina/uso terapêutico , Raios gama/efeitos adversos , Fenóis/uso terapêutico , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/uso terapêutico , 5-Metoxitriptamina/administração & dosagem , Animais , Cistamina/administração & dosagem , Relação Dose-Resposta a Droga , Relação Dose-Resposta à Radiação , Quimioterapia Combinada , Feminino , Injeções Intramusculares , Injeções Intraperitoneais , Masculino , Camundongos , Fenóis/administração & dosagem , Protetores contra Radiação/administração & dosagem , RatosRESUMO
Experiments on mice-hybrids F1(CBA x C57B1/6) have detected a favorable effect of the associated application of quercetin (30-60 minutes before y-exposure of an animal) and a radioprotectant of urgent action indralin (in the case of its application after y-exposure) on a post-irradiation repair of the hematopoietic tissue in acute radiation sickness after y-exposure at a non-lethal dose of 6.7 Gy, which manifested itself in the accelerated formation of endogenous spleen colonies and spleen mass recovery, as well as in the lesser degree of leukopenia on the 12th and the 16th day after acute radiation injury. Quercetin per se did not have a radio-protective effect.
Assuntos
Hematopoese/efeitos da radiação , Fenóis/uso terapêutico , Quercetina/uso terapêutico , Lesões Experimentais por Radiação/sangue , Protetores contra Radiação/uso terapêutico , Doença Aguda , Animais , Relação Dose-Resposta a Droga , Esquema de Medicação , Quimioterapia Combinada , Hematopoese/efeitos dos fármacos , Masculino , Camundongos , Fenóis/administração & dosagem , Quercetina/administração & dosagem , Protetores contra Radiação/administração & dosagem , Baço/efeitos da radiaçãoRESUMO
The investigation involved 307 patients operated on for gastric adenocarcinoma. Nutritional support was given enterally (50), parenterally (98) and by standard methods (159). Enteral nutrition should be recommended because it was followed by relatively higher total protein and serum albumin levels.
Assuntos
Adenocarcinoma/dietoterapia , Apoio Nutricional , Neoplasias Gástricas/dietoterapia , Adenocarcinoma/sangue , Adenocarcinoma/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Nutrição Enteral , Feminino , Humanos , Hipoproteinemia/dietoterapia , Hipoproteinemia/etiologia , Masculino , Pessoa de Meia-Idade , Apoio Nutricional/métodos , Nutrição Parenteral , Desnutrição Proteico-Calórica/dietoterapia , Desnutrição Proteico-Calórica/etiologia , Estudos Retrospectivos , Albumina Sérica/metabolismo , Neoplasias Gástricas/sangue , Neoplasias Gástricas/cirurgia , Fatores de Tempo , Resultado do TratamentoRESUMO
In experiences on white rats at a gamma-irradiation in a lethal dose LD97/30 in conditions of local schielding of an abdomen (in the field of a liver) and application of a radioprotector indraline after irradiation the expressed efficiency of combined protection up to 87.5% is scored at 31.3% of a survival in local schielding of an abdomen group and absence of effect from a drug.
Assuntos
Síndrome Aguda da Radiação/tratamento farmacológico , Raios gama , Equipamentos de Proteção , Lesões Experimentais por Radiação/tratamento farmacológico , Protetores contra Radiação/uso terapêutico , Abdome , Síndrome Aguda da Radiação/prevenção & controle , Animais , Esquema de Medicação , Injeções Intraperitoneais , Masculino , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/efeitos adversos , RatosRESUMO
In experiences on mice F1 (CBAxC57B 1/6) at a gamma-irradiation in a lethal dose LD(35-70/30) the radioprotectant B-190 at administration after an exposure would rise an animal survival--on 35-55%, caused the increase of the amount of endogenic colony in a spleen and of leucocytes in blood on 11th and 30th day of an acute radiation desease accordingly. The drug has the effect in the interval of doses from 75 up to 150 mg/kg b.w. with the rise of radioprotective action on dose reduction factor from 1.1 up to 1.22. alpha(1)-adrenoblockers terasosin in a dose of 15 mg/kg b.w. partially would reduce radioprotective properties of B-190 as radioprotectant or and drug of early therapy of acute radiation desease.
Assuntos
Síndrome Aguda da Radiação/tratamento farmacológico , Raios gama , Lesões Experimentais por Radiação/tratamento farmacológico , Protetores contra Radiação/uso terapêutico , Síndrome Aguda da Radiação/imunologia , Administração Oral , Animais , Relação Dose-Resposta a Droga , Injeções Intraperitoneais , Contagem de Leucócitos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Lesões Experimentais por Radiação/imunologia , Protetores contra Radiação/administração & dosagem , Baço/imunologiaRESUMO
In experiments on mice radioprotective properties of indraline, phenyleprine and melatonin were compared at topical application as an ointment at a place of local exposure of animal hind to a dose of 38.3 Gy of 60Co gamma-quanta. Factor of dose reduction was 1.27-1.32 for indraline (1-10% ointment) and 1.29 for phenyleprine (0.25% ointment). Antioxidants were low efficient at radiation skin burn. At later local radiation injuries, such as hind contracture, the efficiency of indraline was 1.33-1.5, that of phenyleprine was 1.28, and that of melatonine (2 and 5% ointment) was 1.23-1.47.
Assuntos
Antioxidantes/administração & dosagem , Melatonina/administração & dosagem , Fenóis/administração & dosagem , Fenilefrina/administração & dosagem , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/administração & dosagem , Radiodermite/prevenção & controle , Animais , Antioxidantes/uso terapêutico , Radioisótopos de Cobalto/farmacologia , Interpretação Estatística de Dados , Feminino , Melatonina/uso terapêutico , Camundongos , Modelos Teóricos , Pomadas , Fenóis/uso terapêutico , Fenilefrina/uso terapêutico , Doses de Radiação , Protetores contra Radiação/uso terapêutico , Fatores de TempoRESUMO
Effects of cAMP and cGMP dibutyryl derivatives (dbt), calcium ionophore A23187 and the protein kinase C activator PMA phorbol ester on mobility of human blood neutrophils were studied using the Magiskan 2A system of image processing. These drugs were added at concentrations, which allowed to produce similar inhibition of the cells mobility using a pattern of the middle velocity measurement in the cell population: dbt cAMP 5.10(-5)M, A23187 10(-6)M, PMA 10(-5)M; dbt cGMP 5.10(-6)M stimulated the neutrophil mobility. Differences in the patterns of mobility inhibition were detected by means of spectral characteristics related to alteration in the area of individual neutrophils. The drugs with inhibitory action (cAMP, A23187 and PMA) caused a decrease in the spectrum capacity of the area alteration as compared with normal state, while they affected dissimilarly the spectrum frequency characteristics. Dbt cAMP did not alter the frequency characteristics, A23187 shifted the spectrum towards a high frequency region and PMA--towards a low frequency range. Dbt cGMP did not alter the spectrum capacity but shifted it slightly to low frequency region. These alterations in the spectrum frequency characteristics are discussed considering the effect of secondary messengers on three types of the neutrophil movement which exhibit inherent frequencies.
Assuntos
Movimento Celular/fisiologia , Neutrófilos/citologia , Sistemas do Segundo Mensageiro/fisiologia , Humanos , Modelos BiológicosRESUMO
Lipid peroxidation and mineral metabolism were studied in patients with III degree of obesity and bad standing of sodium restricted diet before and after treatment with including in diet of monosodium glutamate (MSG). It was established well being of MSG and absence of negative effect on loss of body mass in course of dietary treatment. MSG caused normalization of level of diene conjugates and some minerals in serum of patients.
Assuntos
Dieta Hipossódica , Aditivos Alimentares/uso terapêutico , Peroxidação de Lipídeos/fisiologia , Minerais/metabolismo , Obesidade/dietoterapia , Glutamato de Sódio/uso terapêutico , Peso Corporal/fisiologia , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Obesidade/metabolismoRESUMO
The radiation risk in aircraft altitude flights was evaluated from calculated dose fields at the altitude of 10-30 km 0-90 degrees North modulated by the galactic cosmic radiation (GCR) in quiescent heliophysical periods. Results of dose measurements in flight are discussed in the light of present-day radiation limits. The authors suggest that pilots should be subsumed under the category of professionals who work with sources of ionizing radiation.
Assuntos
Aeronaves , Altitude , Aviação , Lesões por Radiação/diagnóstico , Humanos , Valores de Referência , Fatores de RiscoRESUMO
The effect of radioprotector indralin on the graft-versus-host reaction was studied on the model of acute GVH disease induced in mice by intraperitoneal transplantation of 40x10(6)semiallogenic splenocytes. The effect was evaluated by animal mortality from GVH disease. Recipients were male F1(CBAxC57Bl/6) mice exposed to 7 Gy 24 h before transplantation. Donors were male C57Bl/6 mice. Indralin, intraperitoneally injected in a dose of 100 mg/kg 5 min after irradiation attenuated the severity of GVH disease. It eliminated phase I of acute GVH reaction and shifted to the right the dynamics of mortality. Estimated time of 50% mortality (LT50) was prolonged by more than 4 days (the parameter increased by 31.1%). Two (5.7%) animals recovered from acute GVH disease, while all controls died. Indralin treatment after irradiation resulted in a 30% increase in survival of exposed mice.
Assuntos
Doença Enxerto-Hospedeiro/tratamento farmacológico , Fenóis/uso terapêutico , Protetores contra Radiação/uso terapêutico , Animais , Transplante de Células/efeitos adversos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Baço/citologiaRESUMO
The dosimetric concept of measurements of electromagnetic radiations (EMR) during irradiation of biological objects can be realized by methods of mathematical modelling of EMR interactions with biological objects, which can be represented as an image of the human body as a uniform ellipsoid of revolution. The efficient surface of EMR absorption for the models shows a marked resonance dependence on the radiation frequency. The ratio of the largest to the smallest axes of the ellipsoid of revolution can be used as a resonance criterion. Besides, radiation polarization needs to be taken into consideration. This paper discusses the frequency dependence of the efficient absorption surface for three major types of radiation polarization. The paper demonstrates an applicability of the calculation method to the evaluation of the EMR absorbed dose rate.
Assuntos
Efeitos da Radiação , Radiação , Absorção , Relação Dose-Resposta à Radiação , Humanos , Matemática , Modelos Biológicos , RadiometriaRESUMO
Experiments were conducted on isolated strips of the guinea pig taenia coli by the double sucrose gap technique. The effect of papaverins on the relaxation kinetics of the polarized and depolarized smooth muscle after contractions induced by electric stimulation was investigated. Depolarized muscle was stimulated by long-lasting hyperpolarizing pulses; contraction arose in response to the termination of the current pulse (off-response). It was found that: 1) the relaxation phase of the depolarized muscle off-response was slowed down as compared with the relaxation phase of the spontaneous contractions in Krebs solution; 2) papeverine accelerates the relaxation phase of the off-response of the depolarized muscle; 3) papaverine does not accelerate the relaxation phase of the evoked contractions in Krebs solution; 4) hyperpolarization and additional depolarization accelerate the relaxation phase of the off-response of the depolarized muscle; 5) hyperpolarization does not accelerate the relaxation phase of the off-response of the depolarized papaverine-treated muscle. The data obtained suggest the existence of the slow electroexcitable calcium channels in the membrane of the smooth muscle cells blocked by papaverine. The calcium influx through these channels slows down the relaxation phase of the depolarized muscle off-response.
Assuntos
Canais Iônicos/efeitos dos fármacos , Contração Muscular/efeitos dos fármacos , Relaxamento Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Papaverina/fisiologia , Animais , Cálcio/metabolismo , Colo/efeitos dos fármacos , Estimulação Elétrica , Cobaias , Técnicas In Vitro , Músculo Liso/metabolismoRESUMO
The paper describes a simple method for registering the relative volume changes of the cells adhering to a glass surface. The changes in the luminosity of cells which followed changes in the osmolarity of the medium were registered by means of microscope with a dark-field condenser and the photoelement connected through the DC amplifier to the continuous ink recorder. It was proved that changes in the cells luminosity really represented the relative cell volume changes. The method allows continuous recording of the amplitude and time course of cell volume changes throughout the experiment with many changes of the solution perfusing the cell chamber, which makes it possible to study the effects of pharmacological agents on the mechanisms of cell volume regulation. Provided that the cell membranes are destroyed by detergents the method allows measurement of the activity of the cell contractile apparatus.