RESUMO
Some microbiological assays and statistical analyses of test results used by the National Center for Antibiotics Analysis are described for the establishment of offical antibiotic reference standards. Examples are given of both cylinder plate agar diffusion assays and turbidimetric assays. Formulas providing simple and quick analyses of data are shown for calculating potency, determining limits for the potency, and performing validity tests on the results.
Assuntos
Antibacterianos/normas , Antibacterianos/análise , Bioensaio/métodos , Nefelometria e Turbidimetria , Estatística como AssuntoAssuntos
Bovinos/metabolismo , Sulfato de Di-Hidroestreptomicina/metabolismo , Penicilinas/metabolismo , Animais , Sulfato de Di-Hidroestreptomicina/administração & dosagem , Sulfato de Di-Hidroestreptomicina/sangue , Sulfato de Di-Hidroestreptomicina/urina , Injeções Intramusculares/veterinária , Injeções Subcutâneas/veterinária , Rim/metabolismo , Fígado/metabolismo , Músculos/metabolismo , Penicilina G Benzatina/administração & dosagem , Penicilina G Benzatina/sangue , Penicilina G Benzatina/metabolismo , Penicilina G Benzatina/urina , Penicilina G Procaína/administração & dosagem , Penicilina G Procaína/sangue , Penicilina G Procaína/metabolismo , Penicilina G Procaína/urina , Penicilinas/administração & dosagemRESUMO
The Microbiological Assay Branch of the National Center for Antibiotics Analysis assays over 100,000 samples of antibiotic products annually, using more than 1000 Ib dehydrated media. The media must be consistently dependable to produce accurate, reliable test results. To assure that the supply of media will meet the established requirements, each lot before purchase is subjected to a series of trials designed to examine growth support, sensitivity, and behavioral and physical factors. Actual antibiotic assays are conducted with the test medium, and performance is rated against a control medium. Controls on the system reduce the variables to allow appraisal of the medium itself.