The role of temperature and carbon dioxide climatic stress factors on the growth kinetics of Escherichia coli.

AIMS: The global level of carbon dioxide and temperature in the atmosphere is expected to increase, which may affect the survival of the stress-adapted bacteria. In this study, the effect of temperature and dissolved carbon dioxide on the growth rate of Escherichia coli-eGFP tagged strain was studied, thus assessing its response to induced environmental stress factors. METHODS AND RESULTS: A kinetic assay has been performed using a microplate reader with a spectrofluorometer to determine the specific growth rates. Polynomial models were developed to correlate the environmental conditions of temperature and carbon dioxide with Escherichia coli BL21 (DE3) growth in culture media and dairy by-products. At a temperature of 42°C, as the dissolved CO2 increased, a decrease in µmax by 0.76 h-1 was observed. In contrast, at 27°C, this increase led to an increase in µmax by 0.99 h-1. Moreover, a correction factor was added when applying the model to dairy whey samples. CONCLUSIONS: The application of this developed model can be considered a useful tool for predicting the growth of Escherichia coli using climate projections.

HPLC Analysis of Phenolic Compounds and Flavonoids with Overlapping Peaks.

The identification and quantification of phenolic compounds and flavonoids in various natural food products is typically conducted using HPLC analysis. Their analysis is particularly complex since most natural food products contain a large number of different phenolic compounds, many of which have similar chemical characteristics such as polarity, which makes complete separation of all eluents extremely difficult. In this work we present and validate a method for the quantitative determination of the concentration of two compounds with similar retention times, i.e. they show overlapping peaks in a mixed solution. Two pairs of phenolic compounds were investigated: caffeic and vanillic acids and ferulic and p-coumaric acids. This technique takes advantage of the different absorbances of the two phenolic compounds in the eluent at various wavelengths and can be used for the quantitative determination of the concentration of these compounds even if they are not separated in the HPLC column. The presented method could be used to interpret the results of HPLC analysis of food products which possess a vast spectrum of phenolic compounds and flavonoids.

A mathematical model for pressure-based organs behaving as biological pressure vessels.

We introduce a mathematical model that describes the allometry of physical characteristics of hollow organs behaving as pressure vessels based on the physics of ideal pressure vessels. The model was validated by studying parameters such as body and organ mass, systolic and diastolic pressures, internal and external dimensions, pressurization energy and organ energy output measurements of pressure-based organs in a wide range of mammals and birds. Seven rules were derived that govern amongst others, lack of size efficiency on scaling to larger organ sizes, matching organ size in the same species, equal relative efficiency in pressurization energy across species and direct size matching between organ mass and mass of contents. The lung, heart and bladder follow these predicted theoretical relationships with a similar relative efficiency across various mammalian and avian species; an exception is cardiac output in mammals with a mass exceeding 10 kg. This may limit massive body size in mammals, breaking Cope's rule that populations evolve to increase in body size over time. Such a limit was not found in large flightless birds exceeding 100 kg, leading to speculation about unlimited dinosaur size should dinosaurs carry avian-like cardiac characteristics.

Modelling the growth of pear postharvest fungal isolates at different temperatures.

The effect of temperature on the mycelium growth kinetics of four postharvest fungal isolates (i.e., Penicillium expansum, Alternaria alternata, Botrytis cinerea and Rhizopus stolonifer) was assessed. A cardinal model with inflection (CMI) was used to describe the effect of the temperature on the growth rate (µ) and the lag time (λ) of each isolate. Cardinal temperature values such as Tmin, Tmax and Topt were estimated and isolates were sorted according to their growth rate and lag time duration. Additionally, model validation was performed on a medium prepared from mashed pear pulp and on artificially wound-inoculated pear fruits. P. expansum was shown to be the most psychotrophic fungus with the lowest estimated Tmin = -8.78. Model validation on pear pulp agar showed growth rate over-prediction in the case of R. stolonifer and B. cinerea but a good correlation in the case of P. expansum and A. alternata. In vivo experiments on pear fruits showed discrepancies from the synthetic and the simulated counterparts for all the fungi with the only exception of P. expansum.

Internal rib structure can be predicted using mathematical models: An anatomic study comparing the chest to a shell dome with application to understanding fractures.

The human rib cage resembles a masonry dome in shape. Masonry domes have a particular construction that mimics stress distribution. Rib cortical thickness and bone density were analyzed to determine whether the morphology of the rib cage is sufficiently similar to a shell dome for internal rib structure to be predicted mathematically. A finite element analysis (FEA) simulation was used to measure stresses on the internal and external surfaces of a chest-shaped dome. Inner and outer rib cortical thickness and bone density were measured in the mid-axillary lines of seven cadaveric rib cages using computerized tomography scanning. Paired t tests and Pearson correlation were used to relate cortical thickness and bone density to stress. FEA modeling showed that the stress was 82% higher on the internal than the external surface, with a gradual decrease in internal and external wall stresses from the base to the apex. The inner cortex was more radio-dense, P < 0.001, and thicker, P < 0.001, than the outer cortex. Inner cortical thickness was related to internal stress, r = 0.94, P < 0.001, inner cortical bone density to internal stress, r = 0.87, P = 0.003, and outer cortical thickness to external stress, r = 0.65, P = 0.035. Mathematical models were developed relating internal and external cortical thicknesses and bone densities to rib level. The internal anatomical features of ribs, including the inner and outer cortical thicknesses and bone densities, are similar to the stress distribution in dome-shaped structures modeled using FEA computer simulations of a thick-walled dome pressure vessel. Fixation of rib fractures should include the stronger internal cortex.

A hypothesis for reactivation of pulmonary tuberculosis: How thoracic wall shape affects the epidemiology of tuberculosis.

This study was aimed at determining the cause for the high incidence of tuberculosis (TB) reactivation occurring in males with a low body mass index (BMI). Current thinking about pulmonary TB describes infection in the lung apex resulting in cavitation after reactivation. A different hypothesis is put forward for TB infection, suggesting that this occurs in subclinical apical cavities caused by increased pleural stress due to a low BMI body habitus. A finite element analysis (FEA) model of a lung was constructed including indentations for the first rib guided by paramedian sagittal CT reconstructions, and simulations were conducted with varying antero-posterior (AP) diameters to mimic chests with a different thoracic index (ratio of AP to the transverse chest diameters). A Pubmed search was conducted about gender and thoracic index, and the effects of BMI on TB. FEA modeling revealed a tenfold increase in stress levels at the lung apex in low BMI chests, and a four-fold increase with a low thoracic index, r(2) = 0.9748 P < 0.001. Low thoracic index was related to BMI, P = 0.001. The mean thoracic index was statistically significantly lower in males, P = 0.001, and increased with age in both genders. This article is the first to suggest a possible mechanism linking pulmonary TB reactivation to low BMI due to the flattened thoracic wall shape of young male adults. The low thoracic index in young males may promote TB reactivation due to tissue destruction in the lung apex from high pleural stress levels.

External rib structure can be predicted using mathematical models: An anatomical study with application to understanding fractures and intercostal muscle function.

As ribs adapt to stress like all bones, and the chest behaves as a pressure vessel, the effect of stress on the ribs can be determined by measuring rib height and thickness. Rib height and thickness (depth) were measured using CT scans of seven rib cages from anonymized cadavers. A Finite Element Analysis (FEA) model of a rib cage was constructed using a validated approach and used to calculate intramuscular forces as the vectors of both circumferential and axial chest wall forces at right angles to the ribs. Nonlinear quadratic models were used to relate rib height and rib thickness to rib level, and intercostal muscle force to vector stress. Intercostal muscle force was also related to vector stress using Pearson correlation. For comparison, rib height and thickness were measured on CT scans of children. Rib height increased with rib level, increasing by 13% between the 3rd and 7th rib levels, where the 7th/8th rib was the widest part or "equator" of the rib cage, P < 0.001 (t-test). Rib thickness showed a statistically significant 23% increase between the 3rd and 7th ribs, P = 0.004 (t-test). Intercostal muscle force was significantly related to vector stress, Pearson correlation r = 0.944, P = 0.005. The three nonlinear quadratic models developed all had statistically significant parameter estimates with P < 0.03. External rib morphology, in particular rib height and thickness, can be predicted using statistical mathematical models. Rib height is significantly related to the calculated intercostal muscle force, showing that environmental factors affect external rib morphology.

Exploring hydrodynamic cavitation for citrus waste valorisation in Malta: from beverage enhancement to potato sprouting suppression and water remediation.

Introduction: The endorsement of circular economy, zero-waste, and sustainable development by the EU and UN has promoted non-thermal technologies in agro-food and health industries. While northern European countries rapidly integrate these technologies, their implementation in Mediterranean food-supply chains remains uncertain. Aims: We evaluated the usefulness of hydrodynamic cavitation (HC) for valorizing orange peel waste in the fresh orange juice supply chain of the Maltese Islands. Method: We assessed: a) the effectiveness of HC in extracting bioactive compounds from orange peels (Citrus sinensis) in water (35°C) and 70% (v/v) ethanol (-10°C) over time, compared to conventional maceration, and b) the potato sprouting-suppression and biosorbent potential of the processed peel for copper, nitrate, and nitrite binding. Results: Prolonged HC-assisted extractions in water (high cavitation numbers), damaged and/or oxidized bioactive compounds, with flavonoids and ascorbic acid being more sensitive, whereas cold ethanolic extractions preserved the compounds involved in radical scavenging. HC-processing adequately modified the peel, enabling its use as a potato suppressant and biosorbent for copper, nitrate, and nitrite. Conclusion: Coupling HC-assisted bioactive compound extractions with using leftover peel for potato-sprouting prevention and as biosorbent for water pollutant removal offers a straightforward approach to promoting circular economic practices and sustainable agriculture in Malta.

The Traffic Light System: A user-friendly alternative for gait data representation.

BACKGROUND: Instrumented gait analysis is an established procedure in biomechanical assessment, requiring specially-trained analysts to interpret the complex graphical output generated. RESEARCH QUESTION: Does a new method of visual representation of lower limb kinematic gait analysis data provide a reliable and valid method of interpretation of biomechanical data for healthcare professionals? METHODS: An innovative system based on the Traffic Lights System (TLS) was developed. Simulated abnormal gait was captured using a 16-camera optoelectronic motion capture system, and the results were presented in both the Traditional Graphical System (TGS) format and the new TLS. An online form was filled by health professionals who attempted to interpret normal and abnormal motion in the joints presented in the 2 output formats. RESULTS: Out of 26 raters, 18 preferred the new system because of its user-friendliness and its ease of interpretation. 2 raters preferred the TGS, with one of these raters clarifying that the preference is due to colour blindness. For intra-rater reliability, 2 trained raters provided a second response for the TGS (Cronbach's Alpha ranging between 0.733 and 0.918), whilst the TLS resulted in Cronbach's Alpha between 0.817 and 1.00 amongst 3 untrained raters. The Fleiss Multi-rater Kappa Test demonstrated low inter-rater reliability amongst raters in the TGS, whereas the overall Fleiss Multi-rater Kappa values of the TLS surpassed the TGS in all 3 studies. SIGNIFICANCE: This study showed that whilst trained health professionals have high intra-rater reliability in interpreting traditional gait analysis results, those professionals inexperienced in the system, do not always comprehend the complex graphs generated by the system when presenting gait analysis data. When these graphs are transformed into coloured outputs representing the extent of the movement, the TLS has demonstrated high validity and high intra- and inter-rater reliability, significantly exceeding those of the TGS, especially in untrained health professionals.

A rapid prototyped atmospheric non-thermal plasma-activated aerosol device and anti-bacterial characterisation.

Non-plasma technologies are being extensively investigated for their potential to mitigate microbial growth through the production of various reactive species. Predominantly, studies utilise atmospheric non-thermal plasma to produce plasma-activated liquids. The advancement of plasma-liquid applications has led to the investigation of plasma-activated aerosols (PAAs). This study aimed to produce a rapid-prototyped plasma-activated aerosol setup and perform chemical and anti-bacterial characterisation on the resultant activated aerosols. The setup was produced using stereolithography 3D printing, and air was used as the carrier gas. The novel design of the device allowed for the direct production of PAAs without the prior generation of plasma-activated water and subsequent aerosolisation. The generated PAAs were assessed for nitrite, hydrogen peroxide and ozone content using colourimetric assays. Anti-bacterial efficacy was tested against three human pathogenic strains: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella enterica. It was observed that nitrite and ozone contact concentration increased with exposure time, yet no hydrogen peroxide was detected. The generated PAAs showed significant zones of no growth for all bacterial strains. These devices, therefore, show potential to be used as anti-bacterial disinfection technologies.

Auxetics and FEA: Modern Materials Driven by Modern Simulation Methods.

Auxetics are materials, metamaterials or structures which expand laterally in at least one cross-sectional plane when uniaxially stretched, that is, have a negative Poisson's ratio. Over these last decades, these systems have been studied through various methods, including simulations through finite elements analysis (FEA). This simulation tool is playing an increasingly significant role in the study of materials and structures as a result of the availability of more advanced and user-friendly commercially available software and higher computational power at more reachable costs. This review shows how, in the last three decades, FEA proved to be an essential key tool for studying auxetics, their properties, potential uses and applications. It focuses on the use of FEA in recent years for the design and optimisation of auxetic systems, for the simulation of how they behave when subjected to uniaxial stretching or compression, typically with a focus on identifying the deformation mechanism which leads to auxetic behaviour, and/or, for the simulation of their characteristics and behaviour under different circumstances such as impacts.

Nanoparticle Food Applications and Their Toxicity: Current Trends and Needs in Risk Assessment Strategies.

ABSTRACT: Nanotechnology has developed into one of the most groundbreaking scientific fields in the last few decades because it exploits the enhanced reactivity of materials at the atomic scale. The current classification of nanoparticles (NPs) used in foods is outlined in relation to the production and physicochemical characteristics. This review aims to concisely present the most popular and widely used inorganic and organic NPs in food industries. Considering that the toxicity of NPs is often associated with chemical reactivity, a series of in vitro toxicity studies are also summarized, integrating information on the type of NP studies and reported specifications, type of cells used, exposure conditions, and assessed end points. The important role of the digestive system in the absorption and distribution of nanoformulated foods within the body and how this affects the resultant cytotoxicity. Examples of how NPs and their accumulation within different organs are presented in relation to the consumption of specific foods. Finally, the role of developing human health risk assessments to characterize both the potential impact of the hazard and the likelihood or level of human exposure is outlined. Uncertainties exist around risk and exposure assessments of NPs due to limited information on several aspects, including toxicity, behavior, and bioaccumulation. Overall, this review presents current trends and needs for future assessments in toxicity evaluation to ensure the safe application of NPs in the food industry.

Controllable Hierarchical Mechanical Metamaterials Guided by the Hinge Design.

In this work, we use computer simulations (Molecular Dynamics) to analyse the behaviour of a specific auxetic hierarchical mechanical metamaterial composed of square-like elements. We show that, depending on the design of hinges connecting structural elements, the system can exhibit a controllable behaviour where different hierarchical levels can deform to the desired extent. We also show that the use of different hinges within the same structure can enhance the control over its deformation and mechanical properties, whose results can be applied to other mechanical metamaterials. In addition, we analyse the effect of the size of the system as well as the variation in the stiffness of its hinges on the range of the exhibited auxetic behaviour (negative Poisson's ratio). Finally, it is discussed that the concept presented in this work can be used amongst others in the design of highly efficient protective devices capable of adjusting their response to a specific application.

Aqueous and gaseous plasma applications for the treatment of mung bean seeds.

Sprouts are particularly prone to microbial contamination due to their high nutrient content and the warm temperatures and humid conditions needed for their production. Therefore, disinfection is a crucial step in food processing as a means of preventing the transmission of bacterial, parasitic and viral pathogens. In this study, a dielectric coplanar surface barrier discharge (DCSBD) system was used for the application of cold atmospheric plasma (CAP), plasma activated water (PAW) and their combination on mung bean seeds. Germination assessments were performed in a test tube set-up filled with glass beads and the produced irrigation water. Overall, it was found that the combined seed treatment with direct air CAP (350 W) and air PAW had no negative impact on mung bean seed germination and growth, nor the concentration of secondary metabolites within the sprouts. These treatments also reduced the total microbial population in sprouts by 2.5 log CFU/g. This research reports for first time that aside from the stimulatory effect of plasma discharge on seed surface disinfection, sustained plasma treatment through irrigation of treated seeds with PAW can significantly enhance seedling growth. The positive outcome and further applications of different forms, of plasma i.e., gaseous and aqueous, in the agro-food industry is further supported by this research.

The Multidirectional Auxeticity and Negative Linear Compressibility of a 3D Mechanical Metamaterial.

In this work, through the use of a theoretical model, we analyse the potential of a specific three-dimensional mechanical metamaterial composed of arrowhead-like structural units to exhibit a negative Poisson's ratio for an arbitrary loading direction. Said analysis allows us to assess its suitability for use in applications where materials must be able to respond in a desired manner to a stimulus applied in multiple directions. As a result of our studies, we show that the analysed system is capable of exhibiting auxetic behaviour for a broad range of loading directions, with isotropic behaviour being shown in some planes. In addition to that, we show that there are also certain loading directions in which the system manifests negative linear compressibility. This enhances its versatility and suitability for a number of applications where materials exhibiting auxetic behaviour or negative linear compressibility are normally implemented.

Impact resistance of composite magnetic metamaterials.

In this work, through numerical studies, we show the possibility of designing composites in a form of magneto-mechanical metamaterials which are capable of exhibiting an enhanced impact resistance in comparison to their non-magnetic counterparts. We also show that it is possible to control the impact resistance of the system solely by means of the magnitude of the magnetic moment associated with magnetic inclusions inserted into the system as well as through the way how magnetic inclusions are distributed within the structure. The latter result is particularly interesting as in this work we show that through the appropriate distribution of magnetic inclusions it is possible to minimise the force that is being transferred to an object through the protective mechanical metamaterial. It is also suggested that the concept proposed in this work can be implemented in the case of already existing protective devices such as military-related protective devices and car bumpers in order to increase their efficiency.

Assessing the efficacy of zinc oxide nanoparticles against Penicillium expansum by automated turbidimetric analysis.

Public concerns about food safety have triggered a worldwide implementation of new legislations aimed at banning many of the most popular food conventional antifungal treatments. There is therefore an urgent need to identify novel and safer solutions to prevent fungal contamination of food. The antifungal effect of zinc oxide nanoparticles (ZnO NPs) against the postharvest pathogenic fungus Penicillium expansum has been investigated in this study. An automated turbidimetric assay, with a standard 96-well microplate, has been developed and optimised regarding the selection of the inoculum size in order to collect sequential optical density measurements. Data were processed by the updated version of the Lambert Pearson model to estimate the minimum inhibitory concentration and the non-inhibitory concentration values which were found to be 9.8 and 1.8 mM (i.e. 798 and 147 ppm), respectively. The current results show that turbidimetry is a reliable technique for assessing the antifungal activity of metal nanoparticles and that zinc oxide (ZnO) is an effective fungicide which can be potentially used to control food safety.

Turbidimetric Assessment of the Growth of Filamentous Fungi and the Antifungal Activity of Zinc Oxide Nanoparticles.

Rapid assessment of fungal growth and screening antifungal compounds, such as nanoparticles (NPs), for effectiveness is a challenging procedure because no primary standards exist as they do for yeasts and bacteria. Because fungi do not grow as single cells, but as hyphal filaments, they cannot be quantified by the usual enumeration techniques used in bacteriology. The growth of three postharvest fungal isolates ( Alternaria alternata, Rhizopus stolonifer, and Botrytis cinerea) was investigated at different inoculum concentrations and in three nutrient media (Sabouraud dextrose agar, potato dextrose agar, and yeast extract dextrose agar [YED]) with a turbidimetric assay. Sequential measurements were performed to generate optical density versus time plots, whereas the growth responses were expressed quantitatively as the generated trapezoidal area. YED medium showed the lowest variation among replicated experiments; potato dextrose agar showed the next lowest, but there was no significant difference. The inoculum size had a minimal effect on the variation of the fungal dynamics. Microscopic assessment of the fungal growth confirmed that YED medium allowed the most homogeneous development of the studied fungi. Therefore, we developed a rapid and reliable technique to evaluate the efficacy of novel antifungal compounds such as zinc oxide NPs. Turbidimetric assessment showed that these NPs were able to inhibit the growth of all three isolates. A. alternata and B. cinerea did not show a significant difference in the level of inhibition at 15 mM, whereas R. stolonifer showed the highest inhibition at the same concentration.

Pathophysiological mechanism of post-lobectomy air leaks.

BACKGROUND: Air leak post-lobectomy continues to remain a significant clinical problem, with upper lobectomy associated with higher air leak rates. This paper investigated the pathophysiological role of pleural stress in the development of post-lobectomy air leak. METHODS: Preoperative characteristics and postoperative data from 367 consecutive video assisted thoracic surgery (VATS) lobectomy resections from one centre were collected prospectively between January 2014 and March 2017. Computer modelling of a lung model using finite element analysis (FEA) was used to calculate pleural stress in differing areas of the lung. RESULTS: Air leak following upper lobectomy was significantly higher than after middle or lower lobectomy (6.3% versus 2.5%, P=0.044), resulting in a significant six-day increase in mean hospital stay, P=0.004. The computer simulation model of the lung showed that an apical bullet shape was subject to eightyfold higher stress than the base of the lung model. CONCLUSIONS: After upper lobectomy, the bullet shape of the apex of the exposed lower lobe was associated with high pleural stress, and a reduction in mechanical support by the chest wall to the visceral pleura due to initial post-op lack of chest wall confluence. It is suggested that such higher stress in the lower lobe apex explains the higher parenchymal air leak post-upper lobectomy. The pleural stress model also accounts for the higher incidence of right-sided prolonged air leak post-resection.