1.
STAR Protoc
; 4(4): 102702, 2023 Dec 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-37948185
RESUMO
Here, we present a multiplexed assay for variant effect protocol to assess the functional impact of all possible genetic variations within a particular genomic region. We describe steps for saturation genome editing by designing and cloning of single-guide RNA (sgRNA). We then detail steps for nucleofection of sgRNA, testing drug response on variants, and amplification of genomic DNA for next-generation sequencing. For complete details on the use and execution of this protocol, please refer to Sahu et al.1.