RESUMO
Mobile introns and inteins self-propagate by 'homing', a gene conversion process initiated by site-specific homing endonucleases. The VMA intein, which encodes the PI-SceI endonuclease in Saccharomyces cerevisiae, is present in several different yeast strains. Surprisingly, a wild wine yeast (DH1-1A) contains not only the intein(+) allele, but also an inteinless allele that has not undergone gene conversion. To elucidate how these two alleles co-exist, we characterized the endonuclease encoded by the DH1-1A intein(+) allele and the target site in the intein(-) allele. Sequence analysis reveals seven mutations in the 31 bp recognition sequence, none of which occurs at positions that are individually critical for activity. However, binding and cleavage of the sequence by PI-SceI is reduced 10-fold compared to the S.cerevisiae target. The PI-SceI analog encoded by the DH1-1A intein(+) allele contains 11 mutations at residues in the endonuclease and protein splicing domains. None affects protein splicing, but one, a R417Q substitution, accounts for most of the decrease in DNA cleavage and DNA binding activity of the DH1-1A protein. Loss of activity in the DH1-1A endonuclease and target site provides one explanation for co-existence of the intein(+) and intein(-) alleles.
Assuntos
Endodesoxirribonucleases/genética , Endodesoxirribonucleases/metabolismo , Endodesoxirribonucleases/fisiologia , Sequências Repetitivas Dispersas , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Alelos , Sequência de Bases , Sítios de Ligação , Candida/genética , Clonagem Molecular , DNA Fúngico/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Mutação , Processamento de Proteína , Eletricidade EstáticaRESUMO
RecA-mediated cleavage of the bacteriophage lambda repressor results in inactivation of the protein and leads to induction of the lambda prophage. Here, we report the identification of three mutations in lambda repressor that significantly increase the rate of RecA-mediated cleavage. These mutations were isolated as intragenic second-site suppressors of a mutation (ind-) which prevents cleavage. Purified repressor proteins that contain both the ind- mutation and one of the second-site mutations undergo cleavage at near wild-type rates. Purified repressors that contain the second-site mutations in otherwise wild-type backgrounds undergo RecA-mediated cleavage at significantly faster rates than wild-type, and form dimers more poorly than the wild-type protein. In related experiments, we found that other repressor mutants that dimerize poorly are also better substrates for RecA-mediated cleavage. Conversely, we show that a covalent disulfide-bonded repressor dimer is resistant to cleavage. These results support a model in which repressor monomers are the only substrate in the cleavage reaction.
Assuntos
Bacteriófago lambda/metabolismo , Recombinases Rec A/metabolismo , Proteínas Repressoras/metabolismo , Fatores de Transcrição/metabolismo , Proteínas Virais/metabolismo , Cinética , Mutação , Proteínas Repressoras/isolamento & purificação , Compostos de Sulfidrila/metabolismo , Supressão Genética , Proteínas Virais/isolamento & purificaçãoRESUMO
Under physiological conditions, lambda repressor can be inactivated in vivo or in vitro by RecA-mediated cleavage of the polypeptide chain. The repressor protein is thought to cleave itself, with RecA acting to stimulate autodigestion. ind- repressor mutants are resistant to RecA-mediated inactivation in vivo. In this paper, we report the purification of 15 ind- repressor proteins and the behaviors of these proteins in the RecA-mediated and autodigestion cleavage reactions. None of these proteins undergoes substantial RecA-dependent cleavage. However, eight mutant proteins autodigest at the same rate as wild-type repressor, six mutants do not autodigest or autodigest slower, and one mutant autodigests faster than wild-type. We discuss these results with respect to repressor structure and RecA-binding, and suggest possible roles for the RecA protein in the cleavage reaction.
Assuntos
Proteínas de Ligação a DNA , Recombinases Rec A/metabolismo , Proteínas Repressoras/metabolismo , Fatores de Transcrição/metabolismo , DNA Viral/metabolismo , Cinética , Mutação , Regiões Operadoras Genéticas , Proteínas Repressoras/genética , Proteínas Repressoras/isolamento & purificação , Proteínas Virais , Proteínas Virais Reguladoras e AcessóriasRESUMO
PI-SceI, a double-stranded DNA endonuclease from Saccharomyces cerevisiae, is generated by protein splicing of an intein, which is an internal polypeptide within a larger precursor protein. The enzyme initiates the mobility of the intein by cleaving at inteinless alleles of the VMA1 gene. Genetic and biochemical studies reveal that the enzyme makes numerous base-specific and phosphate backbone contacts with its 31 bp asymmetrical recognition site. This site can be divided into two regions, both of which contain nucleotides that are essential for cleavage by PI-SceI. Region I contains the PI-SceI cleavage site while Region II includes an adjacent sequence that covers two helical turns. Mutational, interference and DNA mobility shift analyses demonstrate that Region II is sufficient for high-affinity PI-SceI binding. Within this region, PI-SceI uses primarily phosphate backbone and some major groove interactions to contact the DNA, while within Region I, protein binding involves predominantly major groove interactions that overlap and lie proximal to the cleavage site. Interestingly, DNA binding by PI-SceI induces DNA conformational changes within Region II that are entirely exclusive of Region I sequences. Furthermore, additional distortion occurs when PI-SceI binds to Region I in conjunction with Region II. The importance of this latter distortion in the cleavage pathway is underscored by substrate mutations at or near the cleavage site that reduce or eliminate both Region I DNA bending and substrate cleavage. Based on these findings, we propose a model in which sequence-specific contacts made by PI-SceI contribute to its localization to the cleavage site and to its stabilization of a DNA conformation that is required for catalysis. Finally, we discuss how the recognition characteristics of PI-SceI may have allowed the evolution of other endonucleases with altered, but similar, specificities.
Assuntos
DNA/metabolismo , Endodesoxirribonucleases/metabolismo , Processamento de Proteína , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/enzimologia , Sítios de Ligação/genética , DNA/genética , Endodesoxirribonucleases/genética , Mutagênese , Mutação , Saccharomyces cerevisiae/genética , Análise de Sequência de DNA , Especificidade por SubstratoRESUMO
Several protein processing events that involve related chemical mechanisms have been observed in nature. Now, new methods have been developed, based on the same chemical reactions, that permit proteins to be modified in ways that were not previously possible.
Assuntos
Processamento de Proteína , Proteínas/metabolismo , Animais , Proteínas/genéticaRESUMO
Persistence of a mobile DNA element in a population reflects a balance between the ability of the host to eliminate the element and the ability of the element to survive and to disseminate to other individuals. In each of the three biological kingdoms, several families of a mobile DNA element have been identified which encode a single protein that acts on nucleic acids. Collectively termed homing endonuclease genes (HEGs), these elements employ varied strategies to ensure their survival. Some members of the HEG families have a minimal impact on host fitness because they associate with genes having self-splicing introns or inteins that remove the HEGs at the RNA or protein level. The HEG and the intron/intein gene spread throughout the population by a gene conversion process initiated by the HEG-encoded endonuclease called 'homing' in which the HEG and intron/intein genes are copied to cognate alleles that lack them. The endonuclease activity also contributes to a high frequency of lateral transmission of HEGs between species as has been documented in plants and other systems. Other HEGs have positive selection value because the proteins have evolved activities that benefit their host organisms. The success of HEGs in colonizing diverse genetic niches results from the flexibility of the encoded endonucleases in adopting new specificities.
Assuntos
Endonucleases/genética , Evolução Molecular , Genes , Endonucleases/química , Endonucleases/metabolismo , Conversão Gênica , Íntrons/genéticaRESUMO
The lambda cI repressor and the P22 c2 repressor contain two structural domains. In both proteins, the N-terminal domains mediate operator recognition and positive control of transcription, and the C-terminal domains mediate subunit oligomerization and recognition of the recA protein. In some cases, structural, biochemical, and genetic studies implicate particular repressor side chains in these processes.
Assuntos
Bacteriófago lambda/genética , Proteínas de Ligação a DNA , Proteínas Repressoras , Fagos de Salmonella/genética , Fatores de Transcrição , Proteínas Virais , Sequência de Aminoácidos , Sítios de Ligação , Modelos Moleculares , Dados de Sequência Molecular , Regiões Operadoras Genéticas , Conformação Proteica , Proteínas Repressoras/genética , Fatores de Transcrição/genética , Proteínas Virais/genética , Proteínas Virais Reguladoras e AcessóriasRESUMO
In this paper, we report on the isolation and sequence analysis of mutations that confer an induction-deficient phenotype to lambda repressor. A total of 16 different mutations, which occur at 13 different sites in the repressor gene, have been characterized. For most of the mutant lysogens, frequencies of spontaneous induction in a recA+ strain were reduced dramatically in comparison with those for a wild-type phage, and these mutant lysogens showed little or no prophage induction after UV irradiation. The immunity properties of cells containing the mutant repressors show that all of the mutants but one exhibit operator-binding properties indistinguishable from wild-type repressor.
Assuntos
Bacteriófago lambda/genética , Mutação , Recombinases Rec A/farmacologia , Proteínas Repressoras/genética , Fatores de Transcrição/genética , Sequência de Bases , Fenótipo , Ativação ViralRESUMO
The PI-SceI endonuclease from yeast belongs to a protein family whose members contain two conserved dodecapeptide motifs within their primary sequences. The function of two acidic residues within these motifs, Asp218 and Asp326, was examined by substituting alanine, asparagine, and glutamic acid residues at these positions. All of the purified mutant proteins bind to the PI-SceI recognition site with the same affinity and specificity as the wild-type enzyme. By contrast, substituting alanine or asparagine amino acids at the two positions completely eliminates strand cleavage of substrate DNA, whereas substitution with glutamic acid markedly reduces the cleavage activity. Experiments using nicked substrates demonstrate that the wild-type enzyme shows no strand preference during cleavage. These results are consistent with a model in which both acidic residues are part of a single catalytic center that cleaves both DNA strands. Furthermore, substrate binding by wild-type PI-SceI stimulates hydroxyl radical or hydroxide ion attack at the cleavage site while binding by the alanine-substituted proteins either stimulates this attack significantly less or protects the DNA at this position. These finding are discussed in terms of possible reaction mechanisms for PI-SceI-mediated endonucleolytic cleavage.
Assuntos
Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/metabolismo , Endodesoxirribonucleases/química , Endodesoxirribonucleases/metabolismo , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/enzimologia , Sequência de Aminoácidos , Ácido Aspártico , Sequência de Bases , Sítios de Ligação , Sequência Conservada , DNA/química , DNA/metabolismo , Desoxirribonuclease I , Endodesoxirribonucleases/biossíntese , Radical Hidroxila/metabolismo , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Conformação de Ácido Nucleico , Oligodesoxirribonucleotídeos , Oligopeptídeos/química , Plasmídeos , Mutação Puntual , Mapeamento por Restrição , Homologia de Sequência de AminoácidosRESUMO
An unusual protein splicing reaction joins the N-terminal segment (A) and the C-terminal segment (C) of the 119K primary translation product (ABC) of the yeast VMA1 gene to yield a 69K vacuolar H(+)-ATPase subunit (AC) and an internal 50K polypeptide (B). This 50K protein is a site-specific DNA endonuclease that shares 34% identity with the homothallic switching endonuclease. The site cleaved by the VMA1-derived endonuclease exists in a VMA1 allele that lacks the derived endonuclease segment of the open reading frame. Cleavage at this site only occurs during meiosis and initiates 'homing', a genetic event that converts a VMA1 allele lacking the endonuclease coding sequence into one that contains it.
Assuntos
Desoxirribonuclease I/genética , Conversão Gênica , Genes Fúngicos , Meiose/genética , Saccharomyces cerevisiae/genética , Alelos , Sequência de Bases , Deleção Cromossômica , DNA Fúngico/química , Desoxirribonuclease I/metabolismo , Escherichia coli/enzimologia , Escherichia coli/genética , Membranas Intracelulares/enzimologia , Íntrons , Dados de Sequência Molecular , Plasmídeos , ATPases Translocadoras de Prótons/genética , Proteínas Recombinantes/metabolismo , Esporos Fúngicos/fisiologia , Transformação Bacteriana , Vacúolos/enzimologiaRESUMO
The 119-kDa primary translation product of the VMA1 gene of Saccharomyces cerevisiae undergoes a self-catalyzed rearrangement ("protein splicing") that excises an internal 50-kDa segment of the polypeptide and joins the amino-terminal and carboxyl-terminal segments to generate the 69-kDa subunit of the vacuolar membrane-associated H(+)-ATPase. We have shown previously that the internal segment is a site-specific endonuclease (Gimble, F. S., and Thorner, J. (1992) Nature 357, 301-306). Here we describe methods for the high level expression and purification to near homogeneity of both the authentic VMA1-derived endonuclease (or VDE) from yeast (yield 18%) and a recombinant form of VDE made in bacteria (yield 29%). Detailed characterization of these preparations demonstrated that the yeast-derived and bacterially produced enzymes were indistinguishable, as judged by: (a) behavior during purification; (b) apparent native molecular mass (50 kDa); (c) immunological reactivity; and (d) catalytic properties (specific activity; cleavage site recognition; and optima for pH, temperature, divalent cation and ionic strength). The minimal site required for VDE cleavage was delimited to a 30-base pair sequence within its specific substrate (the VMA1 delta vde allele).
Assuntos
Endodesoxirribonucleases/isolamento & purificação , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/enzimologia , Sequência de Bases , Western Blotting , Catálise , Cromatografia em Gel , Primers do DNA , Eletroforese em Gel de Poliacrilamida , Endodesoxirribonucleases/metabolismo , Dados de Sequência Molecular , Peso Molecular , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismoRESUMO
The PI-SceI protein is a member of the LAGLIDADG family of homing endonucleases that is generated by a protein splicing reaction. PI-SceI has a bipartite domain structure, and the protein splicing and endonucleolytic reactions are catalyzed by residues in domains I and II, respectively. Structural and mutational evidence indicates that both domains mediate DNA binding. Treatment of the protein with trypsin breaks a peptide bond within a disordered region of the endonuclease domain situated between residues Val-270 and Leu-280 and interferes with the ability of this domain to bind DNA. To identify specific residues in this region that are involved in DNA binding and/or catalysis, alanine-scanning mutagenesis was used to create a set of PI-SceI mutant proteins that were assayed for activity. One of these mutants, N281A, was >300-fold less active than wild-type PI-SceI, and two other proteins, R277A and N284A, were completely inactive. These decreases in cleavage activity parallel similar decreases in substrate binding by the endonuclease domains of these mutant proteins. We mapped the approximate position of the disordered region to one of the ends of the 31 base pair PI-SceI recognition sequence using mutant proteins that were substituted with cysteine at residues Asn-274 and Glu-283 and tethered to the chemical nuclease FeBABE. These mutational and affinity cleavage data strongly support a model of PI-SceI docked to its DNA substrate that suggests that one or more residues identified here are responsible for contacting base pair A/T(-)(9), which is essential for substrate binding.
Assuntos
Proteínas de Ligação a DNA/metabolismo , DNA/metabolismo , Endodesoxirribonucleases/metabolismo , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Alanina , Sequência de Aminoácidos , Sítios de Ligação , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Endodesoxirribonucleases/química , Endodesoxirribonucleases/genética , Hidrólise , Modelos Moleculares , MutagêneseRESUMO
In somatic mammalian cells, homologous recombination is a rare event. To study the effects of chromosomal breaks on frequency of homologous recombination, site-specific endonucleases were introduced into human cells by electroporation. Cell lines with a partial duplication within the HPRT (hypoxanthine phosphoribosyltransferase) gene were created through gene targeting. Homologous intrachromosomal recombination between the repeated regions of the gene can reconstruct a functioning, wild-type gene. Treatment of these cells with the restriction endonuclease Xba I, which has a recognition site within the repeated region of HPRT homology, increased the frequency or homologous recombination bv more than 10-fold. Recombination frequency was similarly increased by treatment with the rare-cutting yeast endonuclease PI-Sce I when a cleavage site was placed within the repeated region of HPRT. In contrast, four restriction enzymes that cut at positions either outside of the repeated regions or between them produced no change in recombination frequency. The results suggest that homologous recombination between intrachromosomal repeats can be specifically initiated by a double-strand break occurring within regions of homology, consistent with the predictions of a model.
Assuntos
Cromossomos Humanos/genética , Eletroporação/métodos , Endonucleases/administração & dosagem , Recombinação Genética , Sequência de Bases , Sítios de Ligação/genética , Linhagem Celular , DNA/genética , Marcação de Genes , Vetores Genéticos , Humanos , Hipoxantina Fosforribosiltransferase/genética , Dados de Sequência MolecularRESUMO
PI-Scel is a bifunctional yeast protein that propagates its mobile gene by catalyzing protein splicing and site-specific DNA double-strand cleavage. Here, we report the 2.4 A crystal structure of the PI-Scel protein. The structure is composed of two separate domains (I and II) with novel folds and different functions. Domain I, which is elongated and formed largely from seven beta sheets, harbors the N and C termini residues and two His residues that are implicated in protein splicing. Domain II, which is compact and is primarily composed of two similar alpha/beta motifs related by local two-fold symmetry, contains the putative nuclease active site with a cluster of two acidic residues and one basic residue commonly found in restriction endonucleases. This report presents prototypic structures of domains with single endonuclease and protein splicing active sites.
Assuntos
Endodesoxirribonucleases/química , Endodesoxirribonucleases/metabolismo , Processamento de Proteína , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Sítios de Ligação , Evolução Biológica , Cristalografia por Raios X , DNA Fúngico/metabolismo , Eletroquímica , Proteínas Fúngicas/metabolismo , Modelos Moleculares , Estrutura Molecular , Conformação Proteica , Dobramento de Proteína , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/metabolismoRESUMO
Superposition of the PI-SceI and I-CreI homing endonuclease three-dimensional x-ray structures indicates general similarity between the I-CreI homodimer and the PI-SceI endonuclease domain. Saddle-shaped structures are present in each protein that are proposed to bind DNA. At the putative endonucleolytic active sites, the superposition reveals that two lysine (Lys-301 and Lys-403 in PI-SceI and Lys-98 and Lys-98' in I-CreI) and two aspartic acid residues (Asp-218 and Asp-326 in PI-SceI and Asp-20 and Asp-20' in I-CreI) are related by 2-fold symmetry. The critical role of Lys-301, Asp-218, and Asp-326 in the PI-SceI reaction pathway was reported previously. Here, we demonstrate the significance of the active-site symmetry by showing that alanine substitution at Lys-403 reduces cleavage activity by greater than 50-fold but has little effect on the DNA binding activity of the mutant enzyme. Substitution of Lys-403 with arginine, which maintains the positive charge, has only a modest effect on activity. Interestingly, even though the Lys-301 and Lys-403 residues display pseudosymmetry, PI-SceI mutant proteins with substitutions at these positions have different behaviors. The presence of similar basic and acidic residues in many LAGLIDADG homing endonucleases suggests that these enzymes use a common reaction mechanism to cleave double-stranded DNA.
Assuntos
Domínio Catalítico , Enzimas de Restrição do DNA/química , Endodesoxirribonucleases/química , Lisina/análise , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Chlamydomonas/enzimologia , Sondas de DNA/metabolismo , Dimerização , Endodesoxirribonucleases/genética , Magnésio/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Conformação Proteica , Alinhamento de SequênciaRESUMO
A structure-based model describing the interaction of the two-domain PI-SceI endonuclease with its 31-base pair DNA substrate suggests that the endonuclease domain (domain II) contacts the cleavage site region of the substrate, while the protein splicing domain (domain I) interacts with a distal region that is sufficient for high affinity binding. To support this model, alanine-scanning mutagenesis was used to assemble a set of 49 PI-SceI mutant proteins that were purified and assayed for their DNA binding and cleavage properties. Fourteen mutant proteins were 4- to >500-fold less active than wild-type PI-SceI in cleavage assays, and one mutant (T225A) was 3-fold more active. Alanine substitution at two positions in domain I reduces overall binding >60-fold by perturbing the interaction of PI-SceI with the minimal binding region. Conversely, mutations in domain II have little effect on binding, reduce binding to the cleavage site region only, or affect binding to both regions. Interestingly, substitutions at Lys301, which is part of the endonucleolytic active site, eliminate binding to the cleavage site region but permit contact with the minimal binding region. This experimental evidence demonstrates that the protein splicing domain as well as the endonuclease domain is involved in binding of a DNA substrate with the requisite length.
Assuntos
Aminoácidos/metabolismo , DNA/metabolismo , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Splicing de RNA , Aminoácidos/química , Desoxirribonucleases de Sítio Específico do Tipo II/química , Desoxirribonucleases de Sítio Específico do Tipo II/genética , Hidrólise , Mutagênese , Ligação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Proteínas de Saccharomyces cerevisiae , TermodinâmicaRESUMO
The PI-SceI protein is an intein-encoded homing endonuclease that initiates the mobility of its gene by making a double strand break at a single site in the yeast genome. The PI-SceI protein splicing and endonucleolytic active sites are separately located in each of two domains in the PI-SceI structure. To determine the spatial relationship between bases in the PI-SceI recognition sequence and selected PI-SceI amino acids, the PI-SceI-DNA complex was probed by photocross-linking and affinity cleavage methods. Unique solvent-accessible cysteine residues were introduced into the two PI-SceI domains at positions 91, 97, 170, 230, 376, and 378, and the mutant proteins were modified with either 4-azidophenacyl bromide or iron (S)-1-(p-bromoacetamidobenzyl)-ethylenediaminetetraacetate (FeBABE). The phenyl azide-coupled proteins cross-linked to the PI-SceI target sequence, and the FeBABE-modified proteins cleaved the DNA proximal to the derivatized amino acid. The results suggest that an extended beta-hairpin loop in the endonuclease domain that contains residues 376 and 378 contacts the major groove near the PI-SceI cleavage site. Conversely, residues 91, 97, and 170 in the protein splicing domain are in close proximity to a distant region of the substrate. To interpret our results, we used a new PI-SceI structure that is ordered in regions of the protein that bind DNA. The data strongly support a model of the PI-SceI-DNA complex derived from this structure.