RESUMO
600 Golden Hamsters were divided into 3 groups: 1--free from opisthorchis invasion, 2--once infected, 3--repeatedly infected. Seasonal variability of liver, portal and mesentery lymph node and adrenal gland relative mass was investigated in winter, spring and autumn. Diurnal stereotype variability of parenchymatous and lymphoid organ relative mass is associated with a season and invasion number, and in a considerable degree is reflected in the meaning of average diurnal index.
Assuntos
Opistorquíase/patologia , Periodicidade , Glândulas Suprarrenais/patologia , Animais , Peso Corporal , Ritmo Circadiano , Cricetinae , Fígado/patologia , Linfonodos/patologia , Masculino , Tamanho do Órgão , Estações do AnoRESUMO
570 males of golden hamsters were divided into 3 groups: I--free from invasion, II--once infected, III--repeatedly infected. Diurnal activity of cyclic nucleotides and enzyme of pentose-phosphate cycle (G-6-PhDG) was investigated in spring, autumn, and winter at 3, 7, 11 a.m. and 3, 7, 11 p.m. The golden hamsters once infected with opisthorchiasis do not show any seasonal changes in G-6-PhDG activity, but this invasion influences the total content of protein, diurnal oscillation of which depends upon seasons. In spring time the repeated invasion induces the diurnal rhythms of G-6-PhDG and of total protein content. Diurnal stereotype of cyclic nucleotides (cGMP, cAMP) demonstrates the dependence upon seasons and invasion frequency without any changes of the average diurnal level.
Assuntos
Ritmo Circadiano/fisiologia , Glucosefosfato Desidrogenase/sangue , Nucleotídeos Cíclicos/sangue , Opistorquíase/sangue , Via de Pentose Fosfato/fisiologia , Estações do Ano , Animais , Cricetinae , Fígado/metabolismo , Mesocricetus , Opistorquíase/parasitologia , Fatores de TempoRESUMO
By means of immunochemical methods a considerable increase in Ca(2+)-transporting glycoprotein (GP) in liver tissues of golden hamsters at early stages of opisthorchiasis has been established. On the 3rd day after the infection GP amount increases 6 fold, on the 7th day--15 fold. On the 14th day GP amount decreases but does not reach the control level. The control level is reached only on the 22nd day after the infection of animals. Ca(2+)-transporting GP belongs to the system of electrogenic transport of Ca2+ in mitochondria (MCh) and its increase in MCh of the liver is, apparently, the basis of destructive changes of these organelles.
Assuntos
Proteínas de Ligação ao Cálcio/metabolismo , Mitocôndrias Hepáticas/metabolismo , Opistorquíase/metabolismo , Animais , Proteínas de Ligação ao Cálcio/análise , Calibragem , Cricetinae , Imunodifusão , Masculino , Mesocricetus , Mitocôndrias Hepáticas/química , Testes de Precipitina , Fatores de TempoRESUMO
540 golden hamsters were selected into 3 groups: 1--free from invasion, 2--once infected, 3--repeatedly infected with Opisthorchis felineus. Diurnal activity of peripheral blood composition was investigated in spring, autumn and winter at 3, 7, 11 a.m. and 3, 7, 11 p.m. The dependence of biorhythmical organisation of peripheral blood cell composition upon seasons and the opisthorchis invasion frequency was recovered. The eosinophilia is the marker reaction of opisthorchiasis acute phase and it depend upon the invasion frequency. Seasons and invasion frequency affect on all diurnal rhythm components (mezor, acrophase, amplitude) with most largest drift in spring.
Assuntos
Ritmo Circadiano , Opistorquíase/sangue , Periodicidade , Estações do Ano , Animais , Contagem de Células Sanguíneas , Cricetinae , Masculino , Mesocricetus , Fatores de TempoRESUMO
540 male golden hamsters were divided into 3 groups: I--free from invasion, II--once infected, III--repeatedly infected. Diurnal activity of portal lymph node immunocompetent cells was investigated in spring, autumn and winter at 3, 7, 11 a. m. and 3, 7, 11 p. m Peculiarities of diurnal immunocompetent cell activity at on acute opisthorchiasis stage, depending upon a season invasion frequency is determined by immunoreactivity level. Eosinophilic and lymphocytic reactions and the ratio of mitotic activity to degenerative elements are the lymph-node immunocompetence indices.
Assuntos
Ritmo Circadiano , Linfonodos/imunologia , Opistorquíase/imunologia , Periodicidade , Estações do Ano , Animais , Cricetinae , Imunidade Celular , Leucócitos/imunologia , Mesocricetus , Recidiva , Fatores de TempoRESUMO
Opisthorchosis of golden hamster causes the disarrangement of the number and structure of chromosomes of bone marrow cells 15 days after the infection. Analogous changes take place in man's leucocytes under the influence of water-salt extract from adult trematodes. The injury of the chromosomal apparatus advances with the increasing period of the infection and is coordinated with modified immunoreactivity of the organism.
Assuntos
Reações Antígeno-Anticorpo , Aberrações Cromossômicas/genética , Opistorquíase/genética , Animais , Medula Óssea/ultraestrutura , Aberrações Cromossômicas/imunologia , Transtornos Cromossômicos , Cricetinae , Imunidade Celular , Tecido Linfoide/imunologia , Mesocricetus , Opistorquíase/imunologia , Fatores de TempoRESUMO
The investigations conducted on Syrian hamsters have revealed that multiple enteral infusions of cold inactivated (t = -10 degrees C) O. felineus metacercariae resulted in the formation of immunocompetent cell and fibroblast infiltrations around the branches of the portal vein. The infestation of animals by viable pathogens led to reduced accommodation of helminths and suppressed cell infiltration in the liver. It is assumed that the native population of the foci become tolerant to the disease with the penetration of noninvasive Opisthorchis larvae into their body and the development of latent forms of helminthiasis.
Assuntos
Opistorquíase/imunologia , Opisthorchis/patogenicidade , Animais , Cricetinae , Suscetibilidade a Doenças/imunologia , Suscetibilidade a Doenças/parasitologia , Suscetibilidade a Doenças/patologia , Larva/patogenicidade , Fígado/imunologia , Fígado/parasitologia , Fígado/patologia , Masculino , Mesocricetus , Opistorquíase/parasitologia , Opistorquíase/patologia , Fatores de TempoRESUMO
25 women in labour with chronic opisthorchiasis have been examined. They were representatives of native (7), local (8) and migrated population of the focus. Control group comprised 10 women from the local population free of invasion. Their immune status was assessed by the level of IgA, IgG, IgM, circulating immune complexes (CIC) and specific antibodies in the serum obtained from peripheral and funic veins, as determined by enzyme immunoassay (ELISA). To detect opisthorchis antigens in the serum modified radial immunodiffusion test was used. Antibody titres in ELISA, IgG and CIC levels were the highest in migrated women. Antibody titres in ELISA and IgG level in the newborn of migrated women were also considerably higher than in other newborn. Serum IgM level in all groups of newborn was considerably lower than in their mothers and IgA was altogether undetectable. In 3 native and 2 local women the results of immunodiffusion test were positive. It is suggested that transplacental migration of opisthorchis throughout the neonatal period may lead to tolerance formation.