Visually guided associative learning in pediatric and adult migraine without aura.

INTRODUCTION: The Rutgers Acquired Equivalence Test is a visually guided equivalence learning paradigm that involves rule acquisition and generalization. Earlier we found impaired performance in this paradigm among adult migraine patients without aura. The aim of the study was to investigate if similar impairments can be found already in the pediatric form of the disease and to compare the performance of the pediatric study population with that of an adult study population. We hypothesized that the deficits observed in adults would be observable already in the pediatric population. METHODS: Twenty-seven children and adolescents newly diagnosed with migraine without aura and 27 age- and sex-matched healthy controls were tested with the Rutgers Acquired Equivalence Test. Their performance data were compared to each other and those of an earlier adult study population involving 22 patients and 22 age- and sex-matched healthy controls. Four parameters characterizing performance in the two main phases of the paradigm were calculated for each of the four groups. Performance parameters were compared with Mann-Whitney U test. RESULTS: In contrast to the decreased performance of the adult patients in the Rutgers Acquired Equivalence Test, no significant difference was found between pediatric patients and controls in any phase of the paradigm. CONCLUSION: Children living with migraine without aura do not exhibit the same cognitive deficits in the Rutgers Acquired Equivalence Test as their adult counterparts. It can be hypothesized that the deficit of equivalence learning is not an inherent feature of the migrainous cognitive profile, rather the result of the interference of the disease with normal development.

Predicting Stimulus Modality and Working Memory Load During Visual- and Audiovisual-Acquired Equivalence Learning.

Scholars have extensively studied the electroencephalography (EEG) correlates of associative working memory (WM) load. However, the effect of stimulus modality on EEG patterns within this process is less understood. To fill this research gap, the present study re-analyzed EEG datasets recorded during visual and audiovisual equivalence learning tasks from earlier studies. The number of associations required to be maintained (WM load) in WM was increased using the staircase method during the acquisition phase of the tasks. The support vector machine algorithm was employed to predict WM load and stimulus modality using the power, phase connectivity, and cross-frequency coupling (CFC) values obtained during time segments with different WM loads in the visual and audiovisual tasks. A high accuracy (>90%) in predicting stimulus modality based on power spectral density and from the theta-beta CFC was observed. However, accuracy in predicting WM load was higher (≥75% accuracy) than that in predicting stimulus modality (which was at chance level) using theta and alpha phase connectivity. Under low WM load conditions, this connectivity was highest between the frontal and parieto-occipital channels. The results validated our findings from earlier studies that dissociated stimulus modality based on power-spectra and CFC during equivalence learning. Furthermore, the results emphasized the importance of alpha and theta frontoparietal connectivity in WM load.

Impairment of visually guided associative learning in children with Tourette syndrome.

The major symptoms of Tourette syndrome are motor and vocal tics, but Tourette syndrome is occasionally associated with cognitive alterations as well. Although Tourette syndrome does not affect the majority of cognitive functions, some of them improve. There is scarce evidence on the impairment of learning functions in patients with Tourette syndrome. The core symptoms of Tourette syndrome are related to dysfunction of the basal ganglia and the frontostriatal loops. Acquired equivalence learning is a kind of associative learning that is related to the basal ganglia and the hippocampi. The modified Rutgers Acquired Equivalence Test was used in the present study to observe the associative learning function of patients with Tourette syndrome. The cognitive learning task can be divided into two main phases: the acquisition and test phases. The latter is further divided into two parts: retrieval and generalization. The acquisition phase of the associative learning test, which mainly depends on the function of the basal ganglia, was affected in the entire patient group, which included patients with Tourette syndrome with attention deficit hyperactivity disorder, obsessive compulsive disorder, autism spectrum disorder, or no comorbidities. Patients with Tourette syndrome performed worse in building associations. However, the retrieval and generalization parts of the test phase, which primarily depend on the function of the hippocampus, were not worsened by Tourette syndrome.

Power-spectra and cross-frequency coupling changes in visual and Audio-visual acquired equivalence learning.

The three phases of the applied acquired equivalence learning test, i.e. acquisition, retrieval and generalization, investigate the capabilities of humans in associative learning, working memory load and rule-transfer, respectively. Earlier findings denoted the role of different subcortical structures and cortical regions in the visual test. However, there is a lack of information about how multimodal cues modify the EEG-patterns during acquired equivalence learning. To test this we have recorded EEG from 18 healthy volunteers and analyzed the power spectra and the strength of cross-frequency coupling, comparing a unimodal visual-guided and a bimodal, audio-visual-guided paradigm. We found that the changes in the power of the different frequency band oscillations were more critical during the visual paradigm and they showed less synchronized activation compared to the audio-visual paradigm. These findings indicate that multimodal cues require less prominent, but more synchronized cortical contribution, which might be a possible biomarker of forming multimodal associations.

ABCA1 rs2230805 and rs2230806 common gene variants are associated with Alzheimer's disease.

The ATP-binding cassette, sub-family A, member 1 gene (ABCA1) is a relevant positional and functional candidate gene for Alzheimer's disease (AD). A case-control association study of genetic variations covering the ABCA1 locus was performed in relation to AD risk in a Hungarian sample. Five single nucleotide polymorphisms (rs2422493: C-477T, rs2740483: G-17C, rs2230805: G474A/L158L, rs2230806: G656A/R219K and rs2066718: G2311A/V771M) were genotyped in 431 AD patients and 302 cognitively healthy, elderly controls. In single marker analysis, significant associations were found in the case of rs2230805 and rs2230806 polymorphisms: the minor A allele containing genotypes for both polymorphisms were more frequent in the control compared to the AD group. Haplotype analysis revealed that rs2230805, rs2230806 and rs2066718 polymorphisms created a linkage disequilibrium (LD) block with a strong LD between rs2230805 and rs2230806 polymorphisms. In the haplotype risk association tests, A-A-G haplotype of the rs2230805-rs2230806-rs2066718 polymorphisms was found to be nominally significantly more frequent in the control group. After correcting p values for multiple testing, only the effects of the rs2230805 and rs2230806 polymorphisms remained significant in the recessive model suggesting a modest protective effect of their minor alleles in AD, which should be interpreted with considerable caution, until further studies elucidate their role in AD pathology.