RESUMO
The Anacostia River, a Chesapeake Bay tributary running through Washington, D.C., is small but highly polluted with nutrients and contaminants. There is currently a multi-billion dollar tunnel project underway, being built in several phases, aimed at diverting effluent to sewage treatment, especially during high flow periods, and improving water quality of the Anacostia and the river into which it flows, the Potomac. Here, 4 years of biweekly to monthly nutrient and phytoplankton data are analyzed to assess pre-tunnel eutrophication status and relationships to flow conditions. Under all flow conditions, nutrients prior to tunnel implementation were well in excess of values normally taken to be limiting for growth, and hypoxia was apparent during summer. Chlorophyll a was higher in summer (averaging 26.9 µg L-1) than in spring (averaging 14.8 µg L-1), and based on pigment composition, summer communities had proportionately more cyanobacteria (> 2-fold higher zeaxanthin to chlorophyll a ratios) compared to spring, which had proportionately more diatoms (> 2-fold higher fucoxanthin to chlorophyll a ratios). When all data from all years and sites were considered, there was a decrease in diatoms and increase in cyanobacteria with decreasing NO3- and increasing NH4+ concentrations, increasing ratios of NH4+ to NO3-, and increasing temperature. Tunnel implementation and associated nutrient reductions may reduce the severity of summer blooms but reductions of spring assemblages may be even greater because river flows are typically higher at that time of year.
Assuntos
Monitoramento Ambiental , Política Ambiental , Poluição da Água/prevenção & controle , Baías , Clorofila/análise , Clorofila A , Cianobactérias , District of Columbia , Eutrofização , Maryland , Nitrogênio/análise , Fósforo/análise , Fitoplâncton/crescimento & desenvolvimento , Rios/química , Estações do Ano , Poluição da Água/estatística & dados numéricosRESUMO
Human activity causes ocean acidification (OA) though the dissolution of anthropogenically generated CO2 into seawater, and eutrophication through the addition of inorganic nutrients. Eutrophication increases the phytoplankton biomass that can be supported during a bloom, and the resultant uptake of dissolved inorganic carbon during photosynthesis increases water-column pH (bloom-induced basification). This increased pH can adversely affect plankton growth. With OA, basification commences at a lower pH. Using experimental analyses of the growth of three contrasting phytoplankton under different pH scenarios, coupled with mathematical models describing growth and death as functions of pH and nutrient status, we show how different conditions of pH modify the scope for competitive interactions between phytoplankton species. We then use the models previously configured against experimental data to explore how the commencement of bloom-induced basification at lower pH with OA, and operating against a background of changing patterns in nutrient loads, may modify phytoplankton growth and competition. We conclude that OA and changed nutrient supply into shelf seas with eutrophication or de-eutrophication (the latter owing to pollution control) has clear scope to alter phytoplankton succession, thus affecting future trophic dynamics and impacting both biogeochemical cycling and fisheries.
Assuntos
Biodiversidade , Eutrofização , Fitoplâncton/fisiologia , Água do Mar/química , Carbonatos/química , Concentração de Íons de Hidrogênio , Modelos Teóricos , Fitoplâncton/crescimento & desenvolvimentoRESUMO
Harmful algal blooms (HABs), those proliferations of algae that can cause fish kills, contaminate seafood with toxins, form unsightly scums, or detrimentally alter ecosystem function have been increasing in frequency, magnitude, and duration worldwide. Here, using a global modeling approach, we show, for three regions of the globe, the potential effects of nutrient loading and climate change for two HAB genera, pelagic Prorocentrum and Karenia, each with differing physiological characteristics for growth. The projections (end of century, 2090-2100) are based on climate change resulting from the A1B scenario of the Intergovernmental Panel on Climate Change Institut Pierre Simon Laplace Climate Model (IPCC, IPSL-CM4), applied in a coupled oceanographic-biogeochemical model, combined with a suite of assumed physiological 'rules' for genera-specific bloom development. Based on these models, an expansion in area and/or number of months annually conducive to development of these HABs along the NW European Shelf-Baltic Sea system and NE Asia was projected for both HAB genera, but no expansion (Prorocentrum spp.), or actual contraction in area and months conducive for blooms (Karenia spp.), was projected in the SE Asian domain. The implications of these projections, especially for Northern Europe, are shifts in vulnerability of coastal systems to HAB events, increased regional HAB impacts to aquaculture, increased risks to human health and ecosystems, and economic consequences of these events due to losses to fisheries and ecosystem services.
Assuntos
Mudança Climática , Dinoflagellida/crescimento & desenvolvimento , Ecossistema , Previsões/métodos , Proliferação Nociva de Algas/fisiologia , Modelos Biológicos , Geografia , Oceanos e Mares , Movimentos da ÁguaRESUMO
During 2020-2021, an unusually prolonged bloom of the toxigenic dinoflagellate Karenia brevis persisted for more than 12 months along the Gulf coast of Florida, resulting in severe environmental effects. Motivated by the possibility that unusual nutrient conditions existed during summer 2021, the short-term interactions of temperature, nitrogen (N) forms (ammonium (NH4+), nitrate (NO3-), and urea) and availability on photosynthesis-irradiance responses and N uptake rates were examined in summer 2021 and compared to such responses from the earlier winter. Winter samples were exposed to temperatures of 15, 20, 25, 30 °C while summer samples were incubated at 15, 25, 30, 33 °C, representing the maximum range the cells might experience throughout the water column due to daytime surface heating or extreme weather events. Depending on thermal history of the cells, photosynthetic performance differed when cells were exposed to the same temperature, showing a capacity for thermal acclimation in this species. Although blooms generally do not persist throughout the summer, bloom biomass was remarkably higher in summer than during the winter. However, most of the photosynthetic parameters and N uptake rates, as well as total carbon (C) and N cell-1 were significantly lower in the summer populations, showing that the summer populations were photosynthetically and nutritionally stressed. When the summer cells were treated with urea, however, uptake rates and total C and N cell-1 were higher than with the other N substrates, especially in warmer waters, showing differential thermal responses depending on N forms.
Assuntos
Dinoflagellida , Nitrogênio , Temperatura , Água , Fotossíntese , UreiaRESUMO
Phago-mixotrophy, the combination of photoautotrophy and phagotrophy in mixoplankton, organisms that can combine both trophic strategies, have gained increasing attention over the past decade. It is now recognized that a substantial number of protistan plankton species engage in phago-mixotrophy to obtain nutrients for growth and reproduction under a range of environmental conditions. Unfortunately, our current understanding of mixoplankton in aquatic systems significantly lags behind our understanding of zooplankton and phytoplankton, limiting our ability to fully comprehend the role of mixoplankton (and phago-mixotrophy) in the plankton food web and biogeochemical cycling. Here, we put forward five research directions that we believe will lead to major advancement in the field: (i) evolution: understanding mixotrophy in the context of the evolutionary transition from phagotrophy to photoautotrophy; (ii) traits and trade-offs: identifying the key traits and trade-offs constraining mixotrophic metabolisms; (iii) biogeography: large-scale patterns of mixoplankton distribution; (iv) biogeochemistry and trophic transfer: understanding mixoplankton as conduits of nutrients and energy; and (v) in situ methods: improving the identification of in situ mixoplankton and their phago-mixotrophic activity.
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Blooms of dinoflagellate Karlodinium veneficum are widely distributed in estuarine and coastal waters and have been found to cause fish kills worldwide. K. veneficum has a mixed nutritional mode and relies on both photosynthesis and phagotrophy for growth; it is a mixotroph. Here, a model of mixotrophic growth of K. veneficum (MIXO) was developed, calibrated with previously-reported laboratory physiological data, and subsequently embedded in a 3D-coupled hydrodynamic (ROMS)-biogeochemical (RCA) model of eutrophic Chesapeake Bay, USA. The resulting ROMS-RCA-MIXO model was applied in hindcast mode to investigate seasonal and spatial distributions. Simulations showed that K. veneficum blooms occurred during June-August and were confined to the upper and middle Bay, consistent with long-term field observations. Autotrophic growth dominated in spring but heterotrophic growth dominated during the summer. The number of prey ingested by K. veneficum varied from 0.1 to 0.6 day-1 and the food vacuole content reached up to 50% of the core mixotroph biomass. The ingestion rate increased with prey density and also when P:N ratio fell below â¼0.03 (N:P â¼ 33), indicating that K. veneficum only switched to mixotrophic feeding in P-deficient waters when sufficient prey were available; this occurred during the summer months. The digestion rate increased with both the food vacuole content and temperature. The modeling analysis affirms K. veneficum as a phagotrophic 'alga' which is primarily photosynthetic but switches to mixotrophic feeding under nutrient deficient conditions.
Assuntos
Dinoflagellida , Estuários , Animais , Dinoflagellida/fisiologia , PeixesRESUMO
A 2-year study was undertaken to understand feeding preferences of the eastern oyster Crassostrea virginica when growing in conditions of eutrophication and variable flow. Oysters were suspended in the Rhode River, a tributary of Chesapeake Bay, Maryland, USA, and a subset of these oysters was collected monthly, measured in height to determine growth, and the phytoplankton in their gut were examined both microscopically and using indicator pigments and compared with phytoplankton abundance and composition in the water column. The data herein summarize the oyster growth and the gut contents with respect to phytoplankton cell numbers and composition and with respect to signature pigments.
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Planktonic Prorocentrum, common harmful dinoflagellate, are increasing in frequency, duration, and magnitude globally, as exemplified by the number of blooms of P. minimum in Chesapeake Bay that have nearly doubled over the past 3 decades. Although the dynamics of transport and seasonal occurrence of this species have been previously described, it has been challenging to predict the timing and location of P. minimum blooms in Chesapeake Bay. We developed a new three-dimensional mechanistic model of this species that integrates physics, nutrient cycling and plankton physiology and embedded it within a coupled hydrodynamic-biogeochemical model originally developed for simulating water quality in eutrophic estuarine and coastal waters. Hindcast simulations reproduced the observed time series and spatial distribution of cell density, in particular capturing well its peak in May in the mid-to-upper part of the estuary. Timing and duration of the blooms were mostly determined by the temperature-dependent growth function, while mortality due to grazing and respiration played a minor role. The model also reproduced the pattern of overwintering populations, which are located in bottom waters of the lower Bay, and are transported upstream in spring by estuarine flow. Blooms develop in the mid-upper parts of the estuary when these transported cells encounter high nutrient concentrations from the Susquehanna River and favorable light conditions. Diagnostic analysis and model-sensitivity experiments of nutrient conditions showed that high nitrogen:phosphorus conditions favor bloom development. The model also captured the observed interannual variations in the magnitude and spatial distribution of P. minimum blooms.
Assuntos
Baías , Dinoflagellida , Estuários , Eutrofização , Nitrogênio , Estações do AnoRESUMO
Worldwide increases in human and wildlife diseases have challenged ecologists to understand how large-scale environmental changes affect host-parasite interactions. One of the most profound changes to Earth's ecosystems is the alteration of global nutrient cycles, including those of phosphorus (P) and especially nitrogen (N). Along with the obvious direct benefits of nutrient application for food production, anthropogenic inputs of N and P can indirectly affect the abundance of infectious and noninfectious pathogens. The mechanisms underpinning observed correlations, however, and how such patterns vary with disease type, have long remained conjectural. Here, we highlight recent experimental advances to critically evaluate the relationship between environmental nutrient enrichment and disease. Given the interrelated nature of human and wildlife disease emergence, we include a broad range of human and wildlife examples from terrestrial, marine, and freshwater ecosystems. We examine the consequences of nutrient pollution on directly transmitted, vector-borne, complex life cycle, and noninfectious pathogens, including West Nile virus, malaria, harmful algal blooms, coral reef diseases, and amphibian malformations. Our synthetic examination suggests that the effects of environmental nutrient enrichment on disease are complex and multifaceted, varying with the type of pathogen, host species and condition, attributes of the ecosystem, and the degree of enrichment; some pathogens increase in abundance whereas others decline or disappear. Nevertheless, available evidence indicates that ecological changes associated with nutrient enrichment often exacerbate infection and disease caused by generalist parasites with direct or simple life cycles. Observed mechanisms include changes in host/vector density, host distribution, infection resistance, pathogen virulence or toxicity, and the direct supplementation of pathogens. Collectively, these pathogens may be particularly dangerous because they can continue to cause mortality even as their hosts decline, potentially leading to sustained epidemics or chronic pathology. We suggest that interactions between nutrient enrichment and disease will become increasingly important in tropical and subtropical regions, where forecasted increases in nutrient application will occur in an environment rich with infectious pathogens. We emphasize the importance of careful disease management in conjunction with continued intensification of global nutrient cycles.
Assuntos
Doenças Transmissíveis Emergentes/veterinária , Nitrogênio , Fósforo , Animais , Animais Selvagens , Doenças Transmissíveis Emergentes/transmissão , Ecossistema , Fertilizantes , Interações Hospedeiro-Patógeno , Humanos , Modelos Biológicos , ZoonosesRESUMO
Nutrient pollution and greenhouse gas emissions related to crop agriculture and confined animal feeding operations (CAFOs) in the US have changed substantially in recent years, in amounts and forms. This review is intended to provide a broad view of how nutrient inputs-from fertilizer and CAFOs-as well as atmospheric NH3 and greenhouse gas emissions, are changing regionally within the US and how these changes compare with nutrient inputs from human wastewater. Use of commercial nitrogen (N) fertilizer in the US, which now exceeds 12,000,000 metric tonnes (MT) continues to increase, at a rate of 60,000 MT per year, while that of phosphorus (P) has remained nearly constant over the past decade at around 1,800,000 MT. The number of CAFOs in the US has increased nearly 10% since 2012, driven largely by a near 13% increase in hog production. The annualized inventory of cattle, dairy cows, hogs, broiler chickens and turkeys is approximately 8.7 billion, but CAFOs are highly regionally concentrated by animal sector. Country-wide, N applied by fertilizer is about threefold greater than manure N inputs, but for P these inputs are more comparable. Total manure inputs now exceed 4,000,000 MT as N and 1,400,000 MT as P. For both N and P, inputs and proportions vary widely by US region. The waste from hog and dairy operations is mainly held in open lagoons that contribute to NH3 and greenhouse gas (as CH4 and N2O) emissions. Emissions of NH3 from animal waste in 2019 were estimated at > 4,500,000 MT. Emissions of CH4 from manure management increased 66% from 1990 to 2017 (that from dairy increased 134%, cattle 9.6%, hogs 29% and poultry 3%), while those of N2O increased 34% over the same time period (dairy 15%, cattle 46%, hogs 58%, and poultry 14%). Waste from CAFOs contribute substantially to nutrient pollution when spread on fields, often at higher N and P application rates than those of commercial fertilizer. Managing the runoff associated with fertilizer use has improved with best management practices, but reducing the growing waste from CAFO operations is essential if eutrophication and its effects on fresh and marine waters-namely hypoxia and harmful algal blooms (HABs)-are to be reduced.
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Climate projections suggest-with substantial certainty-that global warming >1.5 °C will occur by mid-century (2050). Population is also projected to increase, amplifying the demands for food, fuel, water and sanitation, which, in turn, escalate nutrient pollution. Global projections of nutrient pollution, however, are less certain than those of climate as there are regionally decreasing trends projected in Europe, and stabilization of nutrient use in North America and Australia. In this review of the effects of eutrophication and climate on harmful algae, some of the complex, subtle, and non-intuitive effects and interactions on the physiology of both harmful and non-harmful taxa are emphasized. In a future ocean, non-harmful diatoms may be disproportionately stressed and mixotrophs advantaged due to changing nutrient stoichiometry and forms of nutrients, temperature, stratification and oceanic pH. Modeling is advancing, but there is much yet to be understood, in terms of physiology, biogeochemistry and trophodynamics and how both harmful and nonharmful taxa may change in an uncertain future driven by anthropogenic activities.
Assuntos
Mudança Climática , Eutrofização , Austrália , Europa (Continente) , América do Norte , Oceanos e MaresRESUMO
Ulva prolifera is a macroalgae that forms massive blooms, negatively impacting natural communities, aquaculture operations and recreation. The effects of the natural products, eugenol, ß-myrcene, citral and nonanoic acid on the growth rate, antioxidative defense system and photosynthesis of Ulva prolifera were investigated as a possible control strategy for this harmful taxon. Negative effects on growth were observed with all four chemicals, due to the excessive production of reactive oxygen species and oxidative damage to the thalli. However, the response of U. prolifera under the four chemicals stress was different at the cellular level. ß-myrcene, the most effective compound in terms of growth inhibition, induced oxidative stress as shown by the damage of total antioxidant capacity (T-AOC) and the downregulation of the glutathione-ascorbate (GSH-ASA) cycle which inhibited the antioxidative system. This chemical also inhibited photosynthesis and photoprotection mechanisms in U. prolifera, resulting in growth limitation. In contrast, U. prolifera was less affected by the second tested chemical, eugenol, and showed no significant change on photosynthetic efficiency in the presence of the chemical. The inhibition effects of the third and fourth tested chemicals, nonanoic acid and citralon, on growth and on the antioxidant defense system in U. prolifera were inferior. These results provide a potential avenue for controlling green tides in the future.
Assuntos
Antioxidantes/metabolismo , Feromônios/toxicidade , Fotossíntese/efeitos dos fármacos , Alga Marinha/efeitos dos fármacos , Ulva/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Fotossíntese/fisiologia , Alga Marinha/metabolismo , Alga Marinha/fisiologia , Ulva/crescimento & desenvolvimento , Ulva/metabolismoRESUMO
Few studies have been carried out on benthic dinoflagellates along the Florida Keys, and little is known about their distribution or toxicity in Florida Bay. Here, the distribution and abundance of benthic dinoflagellates was explored in northern and eastern Florida Bay and along the bay and ocean sides of the Florida Keys. Isolates were brought into culture and their toxicity was tested with oyster larvae bioassays. Seven genera were detected, including Prorocentrum, Coolia, Ostreopsis, Amphidinium, Gambierdiscus, Fukuyoa (all included potentially toxic species) and Sinophysis. In general, distribution increased with water temperature and nutrient availability, especially that of phosphate. This study documented the first record of Coolia santacroce in the Florida Keys. Potential toxic effects of Gambierdiscus caribaeus, the abundance of which exceeded 1000 cells g-1 fw at some sites, were established using oyster larvae as a bioassay organism. These findings suggest a potential risk of ciguatera fish poisoning in this area.
Assuntos
Dinoflagellida/isolamento & purificação , Animais , Oceano Atlântico , Baías , Ciguatera , Ciguatoxinas , Florida , TemperaturaRESUMO
Retrospective analysis of water quality monitoring data reveals strong interannual shifts in the spatial distribution of two harmful algal species (Prorocentrum minimum and Karlodinium veneficum) in eutrophic Chesapeake Bay. A habitat model, based on the temperature and salinity tolerance of the two species as well as their nutrient preferences, provides a good interpretation for the observed seasonal progression and spatial distribution of these taxa. It also points to climate-induced variability in the hydrological forcing as a mechanism driving the interannual shifts in the algal distributions: both P. minimum and K. veneficum shift downstream during wetter years but upstream during dry years. Climate downscaling simulations using the habitat model show upstream shifts of the two species in the estuary and longer blooming seasons by the mid-21st century. Salt intrusion due to sea level rise will raise salinity in the estuary and cause these HAB species to migrate upstream, but increasing winter-spring flows may also drive favorable salinity habitat downstream. Warming leads to longer growing seasons of P. minimum and K. veneficum but may suppress bloom habitat during their respective peak bloom periods.
Assuntos
Dinoflagellida , Proliferação Nociva de Algas , Baías , Ecossistema , Estudos RetrospectivosRESUMO
Coastal waters of the United States (U.S.) are subject to many of the major harmful algal bloom (HAB) poisoning syndromes and impacts. These include paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), amnesic shellfish poisoning (ASP), ciguatera fish poisoning (CFP) and various other HAB phenomena such as fish kills, loss of submerged vegetation, shellfish mortalities, and widespread marine mammal mortalities. Here, the occurrences of selected HABs in a selected set of regions are described in terms of their relationship to eutrophication, illustrating a range of responses. Evidence suggestive of changes in the frequency, extent or magnitude of HABs in these areas is explored in the context of the nutrient sources underlying those blooms, both natural and anthropogenic. In some regions of the U.S., the linkages between HABs and eutrophication are clear and well documented, whereas in others, information is limited, thereby highlighting important areas for further research.
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The proposed plan for enrichment of the Sulu Sea, Philippines, a region of rich marine biodiversity, with thousands of tonnes of urea in order to stimulate algal blooms and sequester carbon is flawed for multiple reasons. Urea is preferentially used as a nitrogen source by some cyanobacteria and dinoflagellates, many of which are neutrally or positively buoyant. Biological pumps to the deep sea are classically leaky, and the inefficient burial of new biomass makes the estimation of a net loss of carbon from the atmosphere questionable at best. The potential for growth of toxic dinoflagellates is also high, as many grow well on urea and some even increase their toxicity when grown on urea. Many toxic dinoflagellates form cysts which can settle to the sediment and germinate in subsequent years, forming new blooms even without further fertilization. If large-scale blooms do occur, it is likely that they will contribute to hypoxia in the bottom waters upon decomposition. Lastly, urea production requires fossil fuel usage, further limiting the potential for net carbon sequestration. The environmental and economic impacts are potentially great and need to be rigorously assessed.
Assuntos
Carbono/química , Ecossistema , Fertilizantes/análise , Ureia/química , Ureia/farmacologia , Efeito Estufa , Oceanos e MaresRESUMO
The harmful dinoflagellate, Karlodnium veneficum, has been implicated in fish-kill and other toxic, harmful algal bloom (HAB) events in waters worldwide. Blooms of K. veneficum are known to be related to coastal nutrient enrichment but the relationship is complex because this HAB taxon relies not only on dissolved nutrients but also particulate prey, both of which have also changed over time. Here, applying cross-correlations of climate-related physical factors, nutrients and prey, with abundance of K. veneficum over a 10-year (2002-2011) period, a synthesis of the interactive effects of multiple factors on this species was developed for Chesapeake Bay, where blooms of the HAB have been increasing. Significant upward trends in the time series of K. veneficum were observed in the mesohaline stations of the Bay, but not in oligohaline tributary stations. For the mesohaline regions, riverine sources of nutrients with seasonal lags, together with particulate prey with zero lag, explained 15%-46% of the variation in the K. veneficum time series. For the oligohaline regions, nutrients and particulate prey generally showed significant decreasing trends with time, likely a reflection of nutrient reduction efforts. A conceptual model of mid-Bay blooms is presented, in which K. veneficum, derived from the oceanic end member of the Bay, may experience enhanced growth if it encounters prey originating from the tributaries with different patterns of nutrient loading and which are enriched in nitrogen. For all correlation models developed herein, prey abundance was a primary factor in predicting K. veneficum abundance.
Assuntos
Baías , Dinoflagellida/fisiologia , Proliferação Nociva de Algas , Modelos Biológicos , Monitoramento Ambiental , Toxinas Marinhas , Dinâmica Populacional , Fatores de TempoRESUMO
Eutrophication is a complex process and often associated with not only a change in overall algal biomass but also with a change in biodiversity. Common metrics of eutrophication (e.g., chlorophyll a), total nitrogen (TN) and phosphorus (TP) are not adequate for understanding biodiversity changes, especially those associated with harmful algal bloom (HAB) proliferations. Harmful algae can increase disproportionately with eutrophication, depending on which nutrients change and in what proportion. This paper challenges several classic paradigms in our understanding of eutrophication and associated biodiversity changes. The underlying message is that nutrient proportions and forms can alter biodiversity, even when nutrients are at concentrations in excess of those considered limiting. The global HAB problem is on a trajectory for more blooms, more toxins, more often, in more places. Our approach to management of HABs and eutrophication must consider the broader complexity of nutrient effects at scales ranging from physiological to ecological.
Assuntos
Biodiversidade , Eutrofização , Proliferação Nociva de Algas , Amônia/metabolismo , Biomassa , Cadeia Alimentar , Nitrogênio/metabolismo , Fósforo/metabolismoRESUMO
Noctiluca scintillans (Noctiluca) is a cosmopolitan red tide forming heterotrophic dinoflagellate. In this study, we investigated its ingestion, elemental growth yield and excretion when supplied with different quality food (nutrient-balanced, N-limited and P-limited). Total cellular elemental ratios of Noctiluca were nearly homeostatic, but the ratio of its intracellular NH4+ and PO43- was weakly regulated. Noctiluca thus seems able to differentially allocate N and P to organic and inorganic pools to maintain overall homeostasis, and it regulated its internal N more strongly and efficiently than P. The latter was substantiated by its comparatively stable C:N ratio and compensatory feeding on N-limited prey. Using both starvation experiments and mass balance models, it was found that excretion of C, N, and P by Noctiluca is highly affected by prey nutritional quality. However, based on modeling results, nutrients seem efficiently retained in actively feeding Noctiluca for reproduction rather than directly released as was shown experimentally in starved cells. Moreover, actively feeding Noctiluca tend to retain P and preferentially release N, highlighting its susceptible to P-limitation. Recycling of N and P by Noctiluca may supply substantial nutrients for phytoplankton growth, especially following bloom senescence.
Assuntos
Dinoflagellida/fisiologia , Ingestão de Alimentos/fisiologia , Proliferação Nociva de Algas/fisiologia , Homeostase/fisiologia , Fitoplâncton/fisiologia , Amônia/metabolismo , Carbono/metabolismo , Monitoramento Ambiental , Processos Heterotróficos/fisiologia , Hong Kong , Nitrogênio/deficiência , Nutrientes/metabolismo , Fosfatos/metabolismo , Fósforo/deficiência , Estações do AnoRESUMO
Lake Taihu has suffered an increasing number of cyanobacteria harmful algal blooms (CyanoHABs) over the past three decades, bringing about formidable ecological and economical losses. Efforts to control phosphate (P) and/or nitrogen (N) have been applied to mitigate these blooms, but there has been much less attention paid to N and its different forms on the ecology of the blooms. Therefore, kinetic and nutrient enrichment experiments were conducted to assess N uptake rates under differing conditions, and to examine effects of changes in N forms (NH4+, NO3- and urea) and P availability on phytoplankton community physiology. In 2014 these experiments involved mesocosm enrichments; in 2015 these experiments were conducted over a diurnal period. Both involved measurements of short-term N uptake. The kinetic results showed that the utilization of NH4+, NO3- and urea by Microcystis-dominated communities was not efficient at low ambient substrate concentrations. Maximum uptake rates by these phytoplankton was achieved on NH4+ and these rates were significantly higher than those on NO3- or urea with or without nutrient pretreatment. Moreover in the presence of PO43- enrichment, the maximal uptake velocity of NH4+ substantially increased without evidence of saturation. High amounts of NH4+ may have inhibited or repressed the uptake of NO3- at certain times in these studies. In the diurnal study, dissolved inorganic carbon and pH changed substantially throughout the day. The resulting high pH altered N and P in ways that may help to sustain nutrient cycling for the blooms.