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1.
Acta Clin Belg ; 78(3): 245-247, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35876324

RESUMO

We present the first documented isolation of Wohlfahrtiimonas chitiniclastica from a patient in Belgium. The isolate was identified as W. chitiniclastica using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing. Our methodology corresponds with the previous conclusions on the superior performance of MALDI-TOF MS for bacterial identification. The patient was treated with amoxicillin/clavulanate and was discharged home after wound management. Although the clinical relevance of the isolate in our case is inconclusive, the pathogenicity of such isolate has been described and therefore must be considered as a potential pathogen in chronic and ulcerating wound cultures.


Assuntos
Pé Diabético , Gammaproteobacteria , Humanos , RNA Ribossômico 16S/genética , Bélgica , Gammaproteobacteria/genética , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
2.
Acta Clin Belg ; 78(6): 521-523, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37589240

RESUMO

We present a case of a 80-year-old patient with a catheter-related bloodstream infection caused by Chimaeribacter species. The Chimaeribacter genus represents a novel genus within the Yersiniaceae family. To the best of our knowledge, as of today, nothing is known about the pathogenicity of Chimaeribacter species, nor about the appropriate antimicrobial management. In the present case, we demonstrate, for the first time, a potential clinical relevance of the Chimaeribacter species. Antimicrobial susceptibility data are presented.

3.
Acta Clin Belg ; 76(2): 152-154, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31545159

RESUMO

We present a case of a young man with known urinary tract abnormalities who developed pyelonephritis and bacteremia caused by Haemophilus parainfluenzae. Since routine urine culture usually does not include enriched media for Haemophilus spp., the true incidence of urinary tract infections caused by H. parainfluenzae is currently unknown. Our case, however, demonstrates that H. parainfluenzae is a potential urinary pathogen, at least in patients with urinary tract anomalies. Clinical laboratories should consider expanding their culture efforts to detect unusual pathogens in patients with underlying risk factors.


Assuntos
Bacteriemia , Infecções por Haemophilus , Pielonefrite , Bacteriemia/diagnóstico , Haemophilus , Infecções por Haemophilus/diagnóstico , Haemophilus parainfluenzae , Humanos , Masculino , Pielonefrite/diagnóstico
4.
J Clin Virol ; 33(1): 1-6, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15797358

RESUMO

BACKGROUND: Group A rotavirus genotypes G1, G2, G3 and G4 are the main etiological agents of infantile diarrhea. The G9 rotavirus has recently emerged as a fifth important genotype all over the world. OBJECTIVE: To characterize the VP7 gene of group A rotaviruses from gastroenteritis patients admitted to the Gasthuisberg University Hospital, Leuven, Belgium, during 1999-2003. STUDY DESIGN: Rotavirus antigen was detected in stool specimens using an enzyme immunoassay. G-typing was performed by reverse transcriptase polymerase chain reaction (RT-PCR) amplification and sequencing of the complete VP7 gene. RESULTS: The genotype distribution varied markedly over the four rotavirus years in Belgium. In the 1999-2000 rotavirus year, G1 was the predominating type (72%), and G9 was present in 5% of the rotavirus-positive patients. In the 2000-2001 and 2002-2003 years, G9 appeared as the dominating strain (45% and 53%, respectively). In the 2001-2002 year, between two G9 epidemic years, G1 was dominating (66%) but G9 was still present in 24%. All the G9 isolates were combined with P[8] and shared a high gene sequence similarity (<3% sequence divergence on the nucleotide and amino acid level). Phylogenetic analysis of the VP7 genes revealed that our Belgian G9 strains clustered together with recent G9 strains from all over the world, distinct from the prototype G9 strains isolated in the 1980s. CONCLUSION: Our study indicates that although the first introduction of G9 isolates in the Belgian population was recorded in 1997, G9 strains were able to establish themselves quickly as the predominant genotype. The emergence of G9 as an important pathogen in both developing as industrialized countries necessitates the urgent consideration of the G9 moiety in rotavirus vaccines.


Assuntos
Antígenos Virais/genética , Proteínas do Capsídeo/genética , Gastroenterite/epidemiologia , Hospitalização , Infecções por Rotavirus/epidemiologia , Rotavirus/classificação , Bélgica/epidemiologia , Pré-Escolar , Gastroenterite/virologia , Genótipo , Humanos , Lactente , Recém-Nascido , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Rotavirus/genética , Rotavirus/isolamento & purificação , Infecções por Rotavirus/virologia , Análise de Sequência de DNA
5.
J Clin Microbiol ; 42(4): 1605-8, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15071012

RESUMO

We developed a novel method that uses sodium dodecyl sulfate-EDTA-treated chromatography paper strips to collect unconcentrated fresh stool samples. After the paper strips were stored for 4 months at room temperature, rotavirus RNA could be successfully amplified by using reverse transcriptase PCR. The use of filter paper strips as a specimen support allows (self-)collection of stool samples by untrained persons. Diarrheal stool samples from remote areas can be stored and transported to a central diagnostic laboratory without the need for freezers or special shipping conditions. This convenient and inexpensive rotavirus sample collection system can be of use in epidemiological surveillance studies and vaccine trials.


Assuntos
Cromatografia em Papel , Fezes/virologia , Fitas Reagentes , Rotavirus/isolamento & purificação , Manejo de Espécimes/métodos , Diarreia/virologia , Ácido Edético , Humanos , RNA Viral/análise , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rotavirus/genética , Infecções por Rotavirus/virologia , Dodecilsulfato de Sódio
6.
J Clin Microbiol ; 41(5): 2088-95, 2003 May.
Artigo em Inglês | MEDLINE | ID: mdl-12734253

RESUMO

A binary classification system has been established for group A rotaviruses, with the viral capsid protein VP7 defining G types and VP4 defining P types. At least 15 G types and 21 P types have been isolated globally with various G and P combinations. Most of the currently circulating human rotaviruses belong to G1P[8], G2P[4], G3P[8], and G4P[8]. We report a human rotavirus strain (B1711) with a novel genotypic VP7/VP4 combination of G6P[6]. This unique rotavirus was isolated from a 13-month-old human immunodeficiency virus (HIV)- negative child of an HIV-seropositive Malian mother that was hospitalized with severe diarrhea in Belgium after returning from a trip to Mali. The VP7 and VP4 genes of the rotavirus strain were sequenced, and phylogenetic trees were constructed. Nucleotide and amino acid sequence comparisons with 15 known G genotypes indicated that the VP7 sequence of strain B1711 was most closely related to an American (Se584) and an Italian (PA151) human G6 strain (95 to 96% nucleotide and 98% amino acid identity). Comparison of the VP4 sequence with 21 P types showed the closest similarity to P[6] genotypes, with greatest similarity to a G8P[6] Malawi strain (mw131) (97% nucleotide and 98% amino acid identity). The B1711 strain is the first reported rotavirus isolate with a G6P[6] genotypic combination. The discovery and surveillance of novel human and nonhuman rotavirus G or P types or of novel G/P combinations is essential for the design of future rotavirus vaccines and for our understanding of rotavirus diversity and evolution.


Assuntos
Antígenos Virais , Recombinação Genética , Rotavirus/classificação , Rotavirus/genética , Sequência de Bases , Bélgica , Proteínas do Capsídeo/genética , DNA Viral/genética , Feminino , Gastroenterite/virologia , Genes Virais , Humanos , Lactente , Mali , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rotavirus/isolamento & purificação , Infecções por Rotavirus/virologia , Viagem
7.
Virology ; 325(1): 11-7, 2004 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-15231381

RESUMO

Group A rotaviruses are the main cause of severe diarrhoea in humans and animals throughout the world. We report the first description of a clinically apparent infection with a P[14], G3 rotavirus (strain B4106) in a hospitalized 6-year-old child. The VP7 gene of the B4106 strain had the closest sequence similarity (94% and 97% on the nucleotide and amino acid level, respectively) with strain 30/96 (P[14], G3), a lapine rotavirus isolated in an Italian rabbit in 1996 while the VP4 gene had the closest similarity with strain 30/96 on the nucleotide level (96%), and with lapine strains C-11 (P[14], G3) and Alabama (P[14], G3), isolated in the United States in the 1980s on the amino acid level (99%). The host restriction determinant gene NSP4 of B4106 was also most similar to lapine strain Alabama (95% nt identity and 97% aa identity). Phylogenetic analysis showed that the VP4, VP7, and NSP4 genes of the B4106 strain share a common evolutionary lineage with those of lapine rotavirus strains. We therefore hypothesize that a lapine rotavirus was able to cross the host species barrier and caused disease in a new host. The increasing detection of strains in humans that were previously believed to be restricted to animals raises questions whether interspecies transmission of rotaviruses is a common event in nature.


Assuntos
Antígenos Virais , Infecções por Rotavirus/virologia , Rotavirus/classificação , Sequência de Bases , Proteínas do Capsídeo/química , Criança , Genótipo , Glicoproteínas/química , Humanos , Dados de Sequência Molecular , Filogenia , RNA Viral/análise , Toxinas Biológicas , Proteínas não Estruturais Virais/química
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