RESUMO
The time course of muscular dystrophy on the androgen-sensitive levator ani muscle was compared to that of the diaphragm of dystrophic (mdx) mice aged 1-20 months. Muscle growth, isometric contractile properties and caffeine-induced contractures were determined to assess the hormone myotrophic effect, muscle strength and sarcoplasmic reticulum function, respectively, of both control and dystrophic muscles. Histological analysis of mdx muscles showed variable fiber size, centronucleated cells, infiltration of connective tissue, and necrosis which was less severe in the levator ani than in the diaphragm muscle. Tetanic tension per unit weight in the mdx levator ani was reduced (29%) after aging, while the contraction time remained unchanged. The tetanic tension of the mdx diaphragm muscle decreased with age from 3 to 20 months (20-64%), and the relaxation time was prolonged after aging (22%). Gonadectomy of young adult mdx mice caused atrophy of the levator ani muscle, accelerated muscle wasting, reduced the tetanic force (31%), but it did not affect the contraction time and caffeine responses. The results showed that testosterone does not prevent the progress of muscle disease in the mdx levator ani, but androgen withdrawal accelerates muscle wasting suggesting a normonal beneficial effect.
Assuntos
Androgênios/farmacologia , Distrofia Muscular Animal/fisiopatologia , Fatores Etários , Animais , Animais Recém-Nascidos , Peso Corporal/fisiologia , Cafeína/farmacologia , Estimulantes do Sistema Nervoso Central/farmacologia , Diafragma/crescimento & desenvolvimento , Diafragma/fisiopatologia , Contração Isométrica/efeitos dos fármacos , Contração Isométrica/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos mdx , Tamanho do Órgão/fisiologia , Tempo de Reação/efeitos dos fármacos , Tempo de Reação/fisiologia , Coloração e Rotulagem/métodosRESUMO
Chamaecrista nictans subs. patellaria var. ramosa Irwin and Barneby (Cassia patellaria DC), tambem conhecida pelo nome popular de peninha, e uma erva daninha originaria do Brasil cujo cha e utilizado na medicina popular para eliminacao de calculos renais. O ensaio farmacologico foi feito a fim de testar o efeito do tratamento cronico com extrato fluido da planta, na urolitiase experimental em ratos atraves da inducao de calculo vesical. Os resultados indicam que o extrato fluido da peninha nao apresentou atividade antilitiasica no modelo experimental testado