RESUMO
Extensive biochemical analysis of whole temporal lobe from cases of dementia and controls suggests that Alzheimer's disease is a primary degenerative nerve-cell disorder and not the result of accelerated ageing. There is selective loss of neocortical cholinergic neurones. Transmitter systems apart from the cholinergic system appears to be affected, but to a lesser extent, and there are no significant changes in the caudate nucleus. The change in cholinergic neurones has been confirmed in biopsy samples.
Assuntos
Envelhecimento , Doença de Alzheimer/etiologia , Demência/etiologia , Receptores Colinérgicos , Lobo Temporal/patologia , Idoso , Doença de Alzheimer/metabolismo , Doença de Alzheimer/patologia , Atrofia , Núcleo Caudado/patologia , Contagem de Células , Colina O-Acetiltransferase/deficiência , Feminino , Frutose-Bifosfato Aldolase/deficiência , Glicólise , Humanos , Ácido Hidroxi-Indolacético/deficiência , Masculino , Pessoa de Meia-Idade , Degeneração Neural , Fosfofrutoquinase-1/deficiência , Serotonina/deficiência , Lobo Temporal/metabolismoRESUMO
Choline acetyltransferase activity (presynaptic cholinergic system) and high affinity binding of cholinergic antagonists (postsynaptic cholinergic system) were measured in brain tissue removed after death from both mentally normal and demented old people. Muscarinic receptor binding sites in frontal cortex decreased with advancing years only in old people without appreciable morphological evidence of senile degeration. Preliminary data for temporal lobe suggested that also in Pick's disease the density of receptor binding sites is reduced. The markers are not significantly reduced in cases of mixed senile and vascular dementia. However, in non-vascular senile dementia of the Alzheimer type, there were indications that the presynaptic marker is selectively depleted. Therefore, centrally acting anticholinesterases might be beneficial, particularly in the early stages of the disease.
Assuntos
Fibras Colinérgicas/patologia , Demência/patologia , Lobo Frontal/patologia , Receptores Colinérgicos , Receptores Muscarínicos , Fatores Etários , Idoso , Envelhecimento , Doença de Alzheimer/enzimologia , Doença de Alzheimer/etiologia , Doença de Alzheimer/patologia , Sítios de Ligação , Colina O-Acetiltransferase/deficiência , Inibidores da Colinesterase/uso terapêutico , Demência/tratamento farmacológico , Demência/enzimologia , Demência/etiologia , Lobo Frontal/enzimologia , HumanosRESUMO
Atrophy with ageing of human whole brain, entire temporal lobe, and caudate nucleus was assessed in autopsy specimens, by biochemical techniques. Only the caudate nucleus showed changes. Markers for several neurotransmitter systems were also examined for changes with age. In neocortex and temporal lobe of human brain, small decreases were detected in markers of cholinergic nerve terminals, whereas a large decrease (79%) occurred in the caudate nucleus. Findings were similar in striatum from 3--33-month-old rats. No change occurred in binding of [3H]quinuclidinyl benzilate by human samples. Markers of serotonergic terminals were also unchanged in human and rat brain. By contrast, binding of [3H]lysergic acid diethylamide and [3H]serotonin was decreased (32-81%) in human neocortex and temporal lobe, but not in caudate nucleus. A 43% loss of a marker of gamma-aminobutyrate terminals occurred in human neocortex, while [3H]muscimol binding increased (179%). No changes were detected in markers of catecholamine synapses in temporal lobe or rat striatum. Hence, with human ageing there appears to be a loss of markers of gamma-aminobutyrate neurones intrinsic to neocortex and acetylcholine cells intrinsic to the caudate nucleus, as well as a change in postsynaptic serotonin receptors in neocortex. These losses are accompanied by relative preservation of markers of ascending projections from basal forebrain and brain stem.
Assuntos
Encéfalo/crescimento & desenvolvimento , Idoso , Envelhecimento , Animais , Atrofia , Encéfalo/patologia , Núcleo Caudado/crescimento & desenvolvimento , Núcleo Caudado/patologia , Colina O-Acetiltransferase/metabolismo , Feminino , Humanos , Técnicas In Vitro , Cinética , Masculino , Pessoa de Meia-Idade , Ratos , Receptores de Superfície Celular/metabolismo , Receptores de Serotonina/metabolismo , Serotonina/metabolismo , Especificidade da Espécie , Lobo Temporal/crescimento & desenvolvimento , Lobo Temporal/patologia , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
Markers of serotonin synapses in entire temporal lobe and frontal and temporal neocortex were examined for changes in Alzheimer's disease by use of both neurosurgical and autopsy samples. Uptake of [3H]serotonin, binding of [3H]imipramine, and content of indolamines were all significantly reduced, indicating that serotonin nerve terminals are affected. Binding of [3H]serotonin was also reduced, whereas that of [3H]quinuclidinyl benzilate, [3H]muscimol, and [3H]dihydroalprenolol were unaltered. When the Alzheimer's samples were subdivided according to age, the reduction in [3H]serotonin binding was a feature of only autopsy samples from younger patients. In contrast, presynaptic cholinergic activity was reduced in all groups of Alzheimer's samples, including neurosurgical specimens. Five markers, thought to reflect cerebral atrophy, cytoplasm, nerve cell membrane, and neuronal perikarya were measured in the entire temporal lobe. In Alzheimer's disease the reductions (mean 25%, range 20-35%) were thought to be too large to be due only to loss of structures associated with the presumed cholinergic perikarya in the basal forebrain and monoamine neurones in the brain stem.