Emulsions stabilized by soy protein nanoparticles as potential functional non-dairy yogurts.

BACKGROUND: Rice bran oil and soy protein nanoparticles (SPNs) may be considered as novel functional food ingredients for soy yogurt production. Formulation of soy yogurt with SPNs and rice bran oil, which has significant physiological functions, will convert them into functional food products. This study was conducted to develop rice bran oil-based soy protein nanoparticles emulsion (SPNE) and to evaluate physical properties, antioxidant activities, oxidative stability and microbiological load as well as textural attributes of SPNs incorporated yogurt (SPNY) during storage at 4 °C for 45 days. RESULTS: SPNs were prepared from soy protein isolate of defatted soy flour. Solubilization, crystallization and ultrasonication was carried out six times. After the sixth cycle of repeated solubilizing, crystallization and ultrasonication, the size of nano protein was reduced to 72.42 ± 0.22 nm from 586.72 ± 0.75 nm (after first cycle). Viscosity, penetration values and water-holding capacity of SPNs added to yogurt were decreased with increase in reduction size of SPNs. SPNs added to yogurt exhibited greater antiradical scavenging ability and ferric reducing antioxidant property than control yogurt. Fortified soy yogurt had significant higher oxidative stability and proteolytic activity. CONCLUSION: Fortification of non-dairy food products with SPNs, which has significant physiological functions, convert conventional soy yogurt into functional food products. © 2019 Society of Chemical Industry.

Dermatoglyphic Patterns in Patients Having Impacted and Erupted Third Molars-A Comparative Study.

Background: The dermatoglyphics have been used in several researches for predicting various pathologies of oral cavity like periodontitis, dental caries, impacted teeth, mal-occlusion, developmental defects such as cleft lip and palate, and potentially malignant disorders. Impacted teeth may be associated with various pathologies such as caries, pericoronitis, dentigerous cysts, and keratocystic odontogenic tumors; therefore, predicting impacted teeth through dermatoglyphic patterns can help in diagnosis and future treatment planning of such event. Aims and Objectives: The aim and objective of the study were to evaluate and compare various dermatoglyphic patterns in the patients with the impacted maxillary and/or mandibular third molar (group 1) and patients having erupted maxillary and/or mandibular third molar (group 2). Materials and Methods: This study comprised totally 300 patients, which were divided into two groups, according to erupted or impacted maxillary and/or mandibular third molar visiting outpatient department, who fulfilled the selection criteria for the study. After a comprehensive clinical examination, dermatoglyphic patterns were recorded from the same individuals. Panoramic radiographs were taken to confirm the diagnosis. Data collected were entered in the spreadsheet and analyzed. For all tests, P value was set at <0.05 and was considered statistically significant. Result: There was statistical significance noted in frequencies of whorl pattern in patients of group 1 and loop pattern in patients of group 2. Conclusion: This study concluded that dermatoglyphic patterns can be used to assess the prevalence of impacted wisdom teeth.

Role of Metabolic Risk Factors, Family History, and Genetic Polymorphisms (PPARγ and TCF7L2) on Type 2 Diabetes Mellitus Risk in an Asian Indian Population.

INTRODUCTION: Women with family history of diabetes (FHD) are at significantly increased risk of developing gestational diabetes mellitus which may eventually lead to type 2 diabetes mellitus (T2DM) in later life. OBJECTIVE: This study investigates the role of FHD on metabolic markers and gene polymorphisms and hence on T2DM susceptibility in nondiabetic pregnant women and the subsequent risks in their newborns. MATERIALS AND METHODS: The present study was conducted on 200 healthy (nondiabetic and normotensive) adult Asian Indian women, including 100 with and 100 without FHD, living in and around Kolkata, India. During the gestational period, they were studied twice and followed up till delivery. During delivery, both mothers' venous blood and cord blood were collected to estimate serum CRP, glucose, and lipid profiles of the respective mothers and their newborns. Genotyping of PPARγ and TCF7L2 polymorphisms was done from these blood samples. RESULTS: A comparison of the metabolic variables among the subjects with and without FHD revealed significant differences among them. We also found close relationship between mothers and their newborn babies in terms of both PPARγ (rs1801282) C/G and TCF7L2 (rs7903146) C/T polymorphisms. More specifically, genotyping results for mothers with FHD and their newborn babies showed high concordance in inheritance of alleles: (i) for PPARγ via the risk allele G (74.0%) which is carried over to the newborn babies (64.5%) and (ii) for TCF7L2 via the risk allele T (73.0%) which is carried over to the newborn babies (68.5%). CONCLUSION: This study leads to the conclusion that Asian Indian women population based in Kolkata, India, are ethnically and genetically predisposed to the risk factors of diabetes through FHD, which is reflected in their gestational phase, and it has a significant implication on their birth outcomes.

GST profile expression study in some selected plants: in silico approach.

Glutathione acts as a protein disulphide reductant, which detoxifies herbicides by conjugation, either spontaneously or by the activity of one of a number of glutathione-S-transferases (GSTs), and regulates gene expression in response to environmental stress and pathogen attack. GSTs play roles in both normal cellular metabolisms as well as in the detoxification of a wide variety of xenobiotic compounds, and they have been intensively studied with regard to herbicide detoxification in plants. A newly discovered plant GST subclass has been implicated in numerous stress responses, including those arising from pathogen attack, oxidative stress and heavy-metal toxicity. In addition, plants GSTs play a role in the cellular response to auxins and during the normal metabolism of plant secondary products like anthocyanins and cinnamic acid. The present work involves two in silico analytical approaches-general secondary structure prediction studies of the proteins and detailed signature pattern studies of some selected GST classes in Arabdiopsis thaliana, mustard, maize and bread wheat by standard Bioinformatics tools; structure prediction tools; signature pattern tools; and the evolutionary trends were analyzed by ClustalW. For this purpose, sequences were obtained from standard databases. The work reveals that these proteins are mainly alpha helical in nature with specific signature pattern similar to phosphokinase C, tyrosine kinase and casein kinase II proteins, which are closely related to plant oxidative stress. This study aims to comprehend the relationship of GST gene family and plant oxidative stress with respect to certain specific conserved motifs, which may help in future studies for screening of biomodulators involved in plant stress metabolism.

Hypoglycemic activity of the antioxidant saponarin, characterized as alpha-glucosidase inhibitor present in Tinospora cordifolia.

Tinospora cordifolia, used in anti-diabetic herbal drug preparations, was reported [12] to contain an alpha-glucosidase inhibitor, characterized as saponarin (apigenin-6-C-glucosyl-7-O-glucoside). The leaf extract had appreciable antioxidant and hydroxyl radical scavenging activities and contained the flavonoid in the range of 32.1 +/- 1.5-45.5 +/- 3.5 mg/g of dry solid. Saponarin showed mixed competitive inhibition on activities of alpha-glucosidase and sucrase of different origins. IC(50), Ki and ki' values determined were 48 muM, 8 muM and 19.5 microM respectively for intestinal maltase and 35 microM, 6 microM and 13 microM respectively for intestinal sucrase. When given orally to maltose-fed rat, saponarin showed hypoglycemic activity in the range of 20-80 mg/kg compared to 100-200 mg/kg for acarbose as reported.

Clustering of cardiometabolic risk factors in Asian Indian women: Santiniketan women study.

OBJECTIVE: The aim of the present cross-sectional study was to investigate the clustering of cardiometabolic risk factors in premenopausal and postmenopausal Asian Indian women. METHODS: A total of 214 healthy women (25-65 y) from the Bolpur-Santiniketan area, West Bengal, India, took part in the study. The women were categorized into two groups, namely, premenopausal (n = 161) and postmenopausal (n = 53). Anthropometric measures, namely, minimum waist circumference (WC) and the sum of four (biceps + triceps + subscapular + suprailiac) skinfolds (SF4), were measured accordingly. Intra-abdominal visceral fat (IVF) was also measured. Left arm systolic (SBP) and diastolic (DBP) blood pressure was taken in participants. Metabolic profiles, namely, total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), fasting plasma glucose (FPG), insulin, testosterone, and estrogen, were measured accordingly. RESULTS: The four factors identified in premenopausal women were factor 1: WC, SF4, IVF, TC, and TG; factor 2: HDL, SBP, DBP, and insulin; factor 3: TC, TG, LDL, and testosterone; and factor 4: FPG, testosterone, and estrogen. These four factors cumulatively explained 72.97% of the total phenotypic variation. In postmenopausal women, the four factors identified were factor 1: TC, TG, HDL, LDL, and DBP; factor 2: FPG, SBP, and DBP; factor 3: WC, SF4, and IVF; and factor 4: FPG, insulin, testosterone, and estrogen. These four factors together explained 90.71% of the total phenotypic variation in cardiometabolic risk factors. CONCLUSIONS: No common underlying physiological variables in premenopausal and postmenopausal women indicate that a single risk axis for clustering of cardiometabolic phenotypes is highly unlikely.

Prevalence of cardiovascular disease risk factors in people of Asian Indian origin: Age and sex variation.

BACKGROUND: No study has been undertaken on people of Asian Indian origin to investigate the age and sex variation in the prevalence of cardiovascular disease (CVD) risk factors. OBJECTIVES: To investigate the age and sex variation in the prevalence of CVD risk factors among the people of Asian Indian origin. MATERIALS AND METHODS: A total of 682 (302 males and 380 females) participants aged 25-85 years took part in the study. The subjects were categorized into 4 groups, namely, Group I (25-34 years), Group II (35-44 years), Group III (45-54 years), and Group IV (55 years and above). Height, weight, and the circumferences of minimum waist (MWC) and maximum hip were collected using standard techniques. Waist-hip ratio (WHR) was then calculated. Percentage of body fat (%BF) and body mass index (BMI) were measured using an Omron body fat analyzer. Left arm systolic (SBP) and diastolic (DBP) blood pressure were taken from each participant with the help of an Omron MI digital electronic blood/pulse monitor. Metabolic profiles, namely, total cholesterol (TC), triglyceride (TG), high (HDL), low (LDL), very low-density lipoprotein (VLDL), and fasting blood glucose (FBG) were also measured using an autoanalyzer. RESULTS: One-way analysis of variance revealed significant differences for age, BMI, MWC, WHR, SBP, DBP, TC, TG, LDL, VLDL, and TC:HDL and TG:HDL ratios across the groups. It was observed that there were significant sex-specific group differences (male [χ(2) (12)] =29.22, P < 0.01 and female [χ(2) (12)] =56.69, P < 0.001) for obesity, high BP, high TC, high TG, and high FBG. But no significant group-specific sex difference was evident for either of the risk factors, except for Group IV. CONCLUSION: Age irrespective of sex modulates CVD risk factors and warranted prevention as early as middle age.