Normalized Hip and Knee Strength in Two Age Groups of Adolescent Female Soccer Players.

ABSTRACT: Hannon, JP, Wang-Price, S, Garrison, JC, Goto, S, Bothwell, JM, and Bush, CA. Normalized hip and knee strength in two age groups of adolescent female soccer players. J Strength Cond Res 36(1): 207-211, 2022-Limb symmetry strength measures are used for clinical decision-making considering when an athlete is ready to return to sport after anterior cruciate ligament (ACL) injuries. However, changes in bilateral muscle strength occur after ACL injury resulting in potentially altered limb symmetry calculations. Adolescent female soccer players are at increased risk of sustaining ACL injuries. Published age and sex-matched strength values in this population may be of benefit to clinicians to improve clinical decision-making. The purpose of this study was to establish normative hip and knee strength data of both the dominant and nondominant limbs in adolescent female soccer players. Sixty-four female soccer players (ages 10-18) were enrolled in this study. Subjects were divided by age into 2 groups (group 1: 10-14 years; group 2: 15-18 years). Subjects underwent Biodex isokinetic strength testing at 60°·s-1 and 180°·s-1 to assess quadriceps and hamstring strength. Isometric hip strength (abduction and external rotation) was measured using a hand-held dynamometer. No significant differences were found between groups on either limb in regards to quadriceps or hamstring strength. No significant differences were found between groups on either limb for hip external rotation strength. Significant differences in hip abduction strength were found between groups on the dominant (group 1: 0.21 ± 0.04; group 2: 0.18 ± 0.04; p = 0.014) and nondominant (group 1: 0.21 ± 0.05; group 2: 0.18 ± 0.05; p = 0.019) limbs. The results of this study shed light on normative strength values for a high-risk injury population.

Energy Absorption Contribution Deficits in Participants Following Anterior Cruciate Ligament Reconstruction: Implications for Second Anterior Cruciate Ligament Injury.

CONTEXT: Lower-extremity loading patterns change after anterior cruciate ligament reconstruction (ACLR). However, there is limited research regarding energy absorption contribution (EAC) of athletes following ACLR who reinjure their ACL and those who do not. EAC can be utilized as a measure of joint loading during tasks. DESIGN: Cross-sectional study. METHODS: Three groups of individuals (13 in each group) with matched age, sex, height, weight, and sports were enrolled. Data were collected at time of return-to-sport testing for the 2 ACLR groups. An 8-camera 3D motion capture system with a sampling rate of 120 Hz and 2 force plates capturing at 1200 Hz were used to capture joint motions in all 3 planes during a double-limb jump landing. RESULTS: Participants in the ACLR no reinjury and ACLR reinjury groups had significantly greater hip EAC (55.8 [21.5] and 56.7 [21.2]) compared with healthy controls (19.5 [11.1]), P < .001 and P < .001, respectively. The ACLR no reinjury and ACLR reinjury groups had significantly lower knee EAC (24.6 [22.7] and 27.4 [20.8]) compared with healthy controls (57.0 [12.2]), P < .001 and P < .001, respectively. However, the ACLR reinjury group had significantly lower ankle EAC (15.9 [4.6]) than healthy controls (23.5 [6.6]), whereas there was no statistical difference between the ACLR no reinjury group (19.7 [7.8]) and healthy controls. CONCLUSIONS: Athletes who had a second ACL injury after ACLR, and those without second ACL injury, appear to have similar hip, knee, and ankle joint loading of the surgical limb at return-to-sport testing. Nevertheless, joint loading patterns were significantly different from healthy controls. The study suggests that EAC as a measure of joint loading during a double-limb jump landing at time of return to sport may not be a strong predictor for second injury following ACLR.

Effect of Meniscal Repair on Joint Loading in Athletes With Anterior Cruciate Ligament Reconstruction at 3 Months Following Surgery.

CONTEXT: Joint loading following anterior cruciate ligament reconstruction (ACL-R) is thought to influence long-term outcomes. However, our understanding of the role of meniscus repair at the time of ACL-R on early joint loading is limited. OBJECTIVE: To assess if differences in total energy absorption and energy absorption contribution of the hip, knee, and ankle exist in the early stages of rehabilitation between patients who received an isolated ACL-R and those with concomitant meniscal repairs. DESIGN: Cross-sectional. SETTING: Clinical laboratory. PATIENTS: Fifty-nine human subjects, including 27 who underwent ACL-R and 32 who underwent ACL-R with concomitant meniscal repairs. MAIN OUTCOME MEASURE: The total energy absorption and the energy absorption contribution of each joint of both the involved and uninvolved limbs during a double-limb squat task. RESULTS: There were significant differences in energy absorption contribution between groups at the knee joint (P = .01) and the hip joint (P = .04), but not at the ankle joint (P = .48) of the involved limb. Post hoc analysis indicates that preoperative hip and knee loading differences exist and when you control for preoperative loading (analysis of covariance), the postsurgery difference was not significant. CONCLUSIONS: The results of the study suggest that the additional surgical procedure of MR may not have had negative effects on joint loading during squatting at 12 weeks.

Correlation Among Y-Balance Test-Lower Quarter Composite Scores, Hip Musculoskeletal Characteristics, and Pitching Kinematics in NCAA Division I Baseball Pitchers.

Context: Numerous studies have reported kinematic data on baseball pitchers using three-dimensional (3D) motion analysis, but no studies to date have correlated this data with clinical outcome measures. Objective: To examine the relationship among Y-Balance Test-Lower Quarter (YBT-LQ) composite scores, musculoskeletal characteristics of the hip, and pitching kinematics in National Collegiate Athletic Association (NCAA) Division I baseball pitchers. Design: Cross-sectional. Setting: 3D motion analysis laboratory. Participants: Nineteen healthy male college baseball pitchers. Main Outcome Measures: Internal and external hip passive range of motion, hip abduction strength, YBT-LQ composite scores, and kinematic variables of the pitching motion. Results: Stride length demonstrated a moderate positive correlation with dominant limb YBT-LQ composite score (r = .524, P = .02) and nondominant limb YBT-LQ composite score (r = .550, P = .01), and a weak positive correlation with normalized time to maximal humerus velocity (r = .458, P = .04). Stride length had a moderate negative correlation with normalized time to maximal thorax velocity (r = -.522, P = .02) and dominant hip total rotational motion (TRM; r = -.660, P = .002), and had a strong negative correlation with normalized time from stride foot contact to maximal knee flexion (r = -.722, P < .001). Dominant limb YBT-LQ composite score had a weak negative correlation with hip abduction strength difference (r = -.459, P = .04) and normalized time to maximal thorax velocity (r = -.468, P = .04). Nondominant limb YBT-LQ composite score demonstrated a weak negative correlation with normalized time to maximal thorax velocity (r = -.450, P = .05) and had a moderate negative correlation with dominant hip TRM (r = -.668, P = .001). There were no other significant relationships between the remaining variables. Conclusions: YBT-LQ is a clinical measure that can be used to correlate with hip musculoskeletal characteristics and pitching kinematics in NCAA Division I pitchers.

Lower-Extremity Muscle Activity, Kinematics, and Dynamic Postural Control in Individuals With Patellofemoral Pain.

CONTEXT: Altered lower-extremity muscle activity has been associated with lower-extremity kinematics in individuals with patellofemoral pain (PFP). However, few studies have examined these relationships, and the results are inconsistent. OBJECTIVE: To compare the lower-extremity muscle activity, kinematics, pain level, and reach distance during the anterior reach of the star excursion balance test (SEBT) between participants with PFP and healthy individuals (control [CON] group). DESIGN: Case control. SETTING: Research laboratory. PARTICIPANTS: Twenty-eight (PFP = 14 and CON = 14) participants volunteered. INTERVENTION: Each participant performed 3 maximal voluntary isometric contractions of the gluteus maximus, gluteus medius (GMED), adductor longus (AL), and vastus medialis, and 5 anterior reaches of the SEBT. MAIN OUTCOME MEASURES: Three-dimensional joint kinematics of the hip and knee at the time of touchdown of the SEBT and integrated electromyography of each muscle were recorded during the descent phase of the SEBT. Coactivation ratios between the GMED and AL were calculated (GMED/AL). Pain level was assessed at the baseline and during performance of the SEBT, using a visual analog scale. RESULTS: Participants with PFP demonstrated decreased GMED/AL coactivation ratio (P = .01) and shorter reach distance (P = .01) during anterior reach of the SEBT compared with the CON group. Participants with PFP demonstrated higher pain levels at baseline (P = .03) and during test performance (P < .001) compared with the CON group and increased pain level during the test performance compared with the baseline (P < .001). No other significant differences were observed. CONCLUSIONS: There were alterations in muscle activity during SEBT performance, suggesting that overactivity of AL relative to GMED is a unique neural recruitment pattern in those with PFP. However, hip and knee joint kinematics did not seem to contribute to deficits in the anterior reach distance, suggesting a need for continued assessment of these deficiencies.

Predicting clinical concussion measures at baseline based on motivation and academic profile.

OBJECTIVE: The purpose of this study was to predict baseline neurocognitive and postural control performance using a measure of motivation, high school grade point average (hsGPA), and Scholastic Aptitude Test (SAT) score. DESIGN: Cross-sectional. SETTING: Clinical research center. PARTICIPANTS: Eighty-eight National Collegiate Athletic Association Division I incoming student-athletes (freshman and transfers). INTERVENTIONS: Participants completed baseline clinical concussion measures, including a neurocognitive test battery (CNS Vital Signs), a balance assessment [Sensory Organization Test (SOT)], and motivation testing (Rey Dot Counting). Participants granted permission to access hsGPA and SAT total score. MAIN OUTCOME MEASURES: Standard scores for each CNS Vital Signs domain and SOT composite score. RESULTS: Baseline motivation, hsGPA, and SAT explained a small percentage of the variance of complex attention (11%), processing speed (12%), and composite SOT score (20%). CONCLUSIONS: Motivation, hsGPA, and total SAT score do not explain a significant amount of the variance in neurocognitive and postural control measures but may still be valuable to consider when interpreting neurocognitive and postural control measures.

Altered Knee Loading Following Primary ACL Repair versus ACL Reconstruction.

Background: ACL repair (ACL-r) has recently gained renewed clinical interest for treatment of ACL tears. ACL-r has several potential benefits over ACL reconstruction (ACL-R) including maintaining the native ACL innervation and blood supply, no graft site morbidity, and possible improved knee biomechanics and decrease in osteoarthritis. The purpose of this study was to assess for differences in metrics of knee joint loading during a single limb squat task between individuals following a primary ACL-r versus those who underwent a standard ACL-R with a patella bone-tendon-bone autograft. Study type: Case Control Study. Methods: The ACL-r group [n: 15, age(yrs): 38.8±13.9] sustained a proximal ACL disruption that was amenable to repair, while the ACL-R group [n: 15, age(yrs): 25.60±1.7] underwent primary reconstruction with patella bone-tendon-bone autograft. At 12-weeks post-operation, both groups completed the IKDC questionnaire and biomechanical testing during performance of the single limb squat. Bilateral peak knee extension moment and total knee joint power as a measure of eccentric loading (contraction) during the descent phase of the squat were calculated on the surgical and non-surgical limb and averaged across the middle three of five trials. Participants also completed quadriceps strength testing on both limbs three months after surgery on an isokinetic dynamometer at 60°/sec. LSI (Limb Strength Index) was calculated for all variables. Separate ANCOVAs were performed on each biomechanical variable to examine differences between groups. Results: The ACL-r had a significantly greater peak knee extension moment LSI (ACL-r: 78.46±5.79%; ACL-R: 56.86±5.79%; p=0.019, ηp2=.186) and total knee joint power LSI (ACL-r: 72.47±7.39%; ACL-R: 39.70±7.39%, p=0.006, ηp2=.245) than the ACL-R group. The ACL-r also had a significantly greater quadriceps LSI than the ACL-R group (ACL-r: 66.318±4.61%, ACL-R: 48.03±4.61%, p=0.013, ηp2=.206). Conclusions: Individuals following ACL-r demonstrate increased knee joint loading symmetry during a single leg squat task and greater quadriceps strength symmetry at 12 weeks post-surgery compared to those who underwent ACL-R. Level of Evidence: 3.

Effects of Limb Dominance on Patellofemoral Joint Loading During Gait at 12 Weeks After Anterior Cruciate Ligament Reconstruction.

Background: Patellofemoral joint complications have commonly been reported in long-term outcome studies for anterior cruciate ligament reconstruction (ACLR); however, the biomechanics in the early phases of rehabilitation that could be associated with the development of these abnormalities is unclear. Limb dominance may affect the biomechanics of the knee joint in patients after ACLR. Purpose: To compare knee joint loading between surgical and nonsurgical limbs at 12 weeks postoperatively in patients who underwent ACLR on either their dominant limb (ACL-D) or nondominant limb (ACL-ND). Study Design: Controlled laboratory study. Methods: Included were 54 patients (32 ACL-D and 22 ACL-ND). Peak and integrated patellofemoral joint stress (PFJS), peak patellofemoral joint reaction force (PFJRF), and peak knee extension moment (KEM) were assessed during the stance phase of gait while participants walked on a 10-m runway at a self-selected speed. Results: The surgical limb of the ACL-D group had significantly decreased peak PFJS (P < .001), integrated PFJS (P < .001), peak PFJRF (P < .001), and peak KEM (P < .001) compared to the nonsurgical limb. The surgical limb of the ACL-ND group demonstrated significantly increased peak PFJS (P = .001), integrated PFJS (P = .023), peak PFJRF (P < .001), and peak KEM (P = .001) compared to the nonsurgical limb. For the surgical limb, the ACL-ND group demonstrated significantly greater peak PFJS (P < .001), peak PFJRF (P < .001) , (PFJRF [P<.001]) and peak KEM (P < .001) than the ACL-D group. For the nonsurgical limb, the ACL-D group demonstrated greater peak PFJS (P < .001), integrated PFJS (P = .023), peak PFJRF (P = .003), and peak KEM (P < .001) than the ACL-ND group. Conclusion: Significantly larger knee joint loading on the surgical limb of the ACL-ND group and smaller knee joint loading on the surgical limb of the ACL-D group were observed compared to the contralateral nonsurgical limb, which suggests that limb dominance has a key role in loading at the knee joint during gait. Clinical Relevance: Altered knee joint loading during gait at 12 weeks after ACLR may lead to the development of patellofemoral joint abnormalities.

Adolescent Athletes Demonstrate Inferior Objective Profiles at the Time of Return to Sport After ACLR Compared With Healthy Controls.

BACKGROUND: Athletes display persistent muscle deficits and altered limb-loading mechanics at the time of return to sport (RTS) after anterior cruciate ligament reconstruction (ACLR). PURPOSE: To compare an objective profile of adolescent athletes at RTS after ACLR to matched healthy controls. STUDY DESIGN: Cross-sectional study; Level of evidence, 3. METHODS: Included were 124 participants; 62 patients who underwent ACLR (15.4 ± 1.7 years) and 62 healthy controls (15.3 ± 1.7 years). Motion capture and force plates were used to capture joint motions during jump landing (JL) and single-limb squat (SLS) tasks. Energy absorption contribution (EAC) was calculated, and repeated-measures analysis of variance was used to assess for EAC differences between groups. Participants completed an International Knee Documentation Committee (IKDC) Subjective Knee Form, and isokinetic quadriceps and hamstring strength testing was performed on each limb. Independent t tests were run to examine age, height, weight, and IKDC scores as well as compare differences between groups for quadriceps and hamstring strength. RESULTS: A significant group × joint interaction was found for JL (P < .001) and SLS (P < .001). For JL, patients who underwent ACLR utilized significantly greater hip (P < .001) and significantly less knee (P < .001) EAC on the surgical limb compared with controls. During SLS, patients who underwent ACLR utilized significantly greater hip (P < .001) and significantly less knee (P < .001) EAC on the surgical limb compared with controls. The ACLR cohort demonstrated lower IKDC scores (P < .001) and significantly lower quadriceps strength on the surgical limb (P < .001) than controls. There were no differences in surgical limb hamstring strength between the ACLR cohort and healthy controls (P = .701). CONCLUSION: Compared with matched healthy controls, the participants who underwent ACLR in this study demonstrated an inferior objective profile at RTS, consisting of deficits in surgical limb loading, self-reported outcomes, and strength.

Movement Patterns During a Jump-Landing Task in Athletes After Sport-Related Concussion and Healthy Control Individuals.

CONTEXT: A relationship between a history of sport-related concussion (SRC) and lower extremity injury has been well established in the literature. OBJECTIVE: To determine if biomechanical differences existed during a double-limb jump landing between athletes who had been released to return to play after SRC and healthy matched control individuals. DESIGN: Cross-sectional study. SETTING: Health system-based outpatient sports medicine center. PATIENTS OR OTHER PARTICIPANTS: A total of 21 participants with SRC (age = 15.38 ± 1.77 years, height = 169.23 ± 8.59 cm, mass = 63.43 ± 7.39 kg, time since release to return to sport after SRC = 16.33 ± 12.7 days) were compared with 21 age-, sex-, and activity-matched healthy participants serving as controls (age = 15.36 ± 1.73 years, height = 169.92 ± 11.1 cm, mass = 65.62 ± 12.08 kg). MAIN OUTCOME MEASURE(S): Biomechanical performance during the double-limb jump landing was assessed using a motion-capture system and force plates. The average of 3 consecutive trials was used to calculate lower extremity joint kinetics and kinematics. The variables of interest were internal knee-extension moment, internal varus moment, and total sagittal-plane knee displacement for the dominant and nondominant limbs. Independent t tests were performed to examine the differences between SRC and control groups for the variables of interest. RESULTS: No differences existed between groups for the descriptive data. The SRC group demonstrated greater internal knee-extension moments in the dominant (-0.028 ± 0.009 Nm/kg, P = .003) and nondominant (-0.018 ± 0.007, P = .02) limbs. The SRC group also exhibited greater internal varus moments in the dominant (0.012 ± 0.004 Nm/kg, P = .005) and nondominant (0.010 ± 0.003, P = .005) limbs. For sagittal-plane knee displacement, the SRC group displayed less knee-flexion displacement in the dominant (-12.56 ± 4.67°, P = .01) but not the nondominant (-8.30 ± 4.91°, P = .10) limb. CONCLUSIONS: Athletes who had been released for return to sport after SRC landed with greater knee valgus than healthy matched control participants.

Female Adolescent Soccer Players Utilize Different Neuromuscular Strategies Between Limbs During the Propulsion Phase of a Lateral Vertical Jump.

BACKGROUND: Multiplanar dynamic stability is an important unilateral function in soccer performance but has been scarcely examined in female soccer players. The lateral vertical jump task assesses unilateral functional performance, and energy generation contribution examines how each joint (hip, knee, ankle) contributes to the vertical component of the vertical jump phase to measure inter- and intra-limb differences. PURPOSE: To examine dominant versus non-dominant limb performance using energy generation contribution of the hip, knee, and ankle during the vertical jump component of the lateral vertical jump. STUDY DESIGN: Cross-sectional observational study. METHODS: Seventeen healthy, adolescent female soccer players (age 13.4±1.7 years; height 160.6±6.0 cm; mass 53.1±8.2 kg) participated. Quadriceps strength was measured via isokinetic dynamometry. Energy generation contribution (measured from maximal knee flexion to toe off) and vertical jump height were measured during the vertical component of the lateral vertical jump. RESULTS: There was no significant difference between limbs for quadriceps strength (p=0.64), jump height (p=0.59), or ankle energy generation contribution (p=0.38). Energy generation contribution was significantly greater in the dominant hip (dominant 29.7±8.6%, non-dominant 18.4±6.3%, p<0.001) and non-dominant knee (dominant 22.8±6.8%, non-dominant 36.2±8.5%, p<0.001). CONCLUSION: High demand on coordination and motor control during the lateral vertical jump and inherent limb dominance may explain different intra-limb strategies for task performance despite jump height symmetry. Non-dominant affinity for stability and dominant compensatory performance may neutralize potential asymmetries. Implications for symmetry in observable outcomes such as jump height must consider underlying internal asymmetries. LEVELS OF EVIDENCE: 3B. CLINICAL RELEVANCE: Symmetrical findings on functional tasks have underlying internal asymmetries observed here in female adolescent soccer players. The lateral vertical jump may highlight these internal asymmetries (hip- versus knee-dominant movement strategies) due to the high coordinative demand to perform the task. Clinicians should be cognizant of underlying, potentially inherent, asymmetries even when observing functional symmetry in a task. WHAT IS KNOWN ABOUT THE SUBJECT: Female adolescent soccer players are a high-risk cohort for sustaining anterior cruciate ligament injuries. Limb dominance may play a role in the performance of functional tasks, and limb dominance in soccer players is quite specialized: the dominant limb is the preferred kicking limb, while the non-dominant limb is the preferred stabilizing limb (plant leg). Functional performance in female soccer players has been studied in kicking, dribbling, sprinting, change of direction, and jumping - however, these tasks were measured independent of limb dominance. It remains to be seen how unilateral functional tasks may be affected by limb dominance in female adolescent soccer players. WHAT THIS STUDY ADDS TO EXISTING KNOWLEDGE: This study provides data on functional performance relative to limb dominance in female adolescent soccer players, and captures the lateral vertical jump task in both inter- and intra-limb measures. This highlights that intra-limb strategies to perform a coordinated motor task may be different between limbs, herein attributed to limb dominance. Even if gross motor outputs between limbs are symmetrical (i.e. jump height), the underlying movement strategies to achieve that output may be different (hip- versus knee-dominant movement strategies). These findings are important to research on functional performance measures related to attaining between-limb symmetry, as measures of energy generation contribution open the door for a more thorough understanding of joint-by-joint intra-limb contributions during a functional task.

Limb dominance influences energy absorption contribution (EAC) during landing after anterior cruciate ligament reconstruction.

OBJECTIVE: To determine the role of limb dominance on energy absorption contribution (EAC) during a jump landing (JL) task at return to sport (RTS) after ACL-R. DESIGN: Cross-sectional study. SETTING: Clinical Research Laboratory. PARTICIPANTS: One hundred eight participants (age = 16.19 ± 1.74, Height = 172.25 ± 9.96 cm, Weight = 72.61 ± 15.48 kg). MAIN OUTCOME MEASURES: Participants were grouped into two groups: those who injured their dominant limb ACL (D-ACL) and those who injured their non-dominant limb ACL (ND-ACL). A multiple analysis of variance (MANOVA) was used to assess for between group differences in EAC across the three joints. RESULTS: In the surgical limb, D-ACL demonstrated smaller hip (D-ACL = 32.23 ± 10.44%, ND-ACL = 69.68 ± 8.51%, p < 0.008) and greater knee (D-ACL = 45.86 ± 10.36%, ND-ACL = 9.41 ± 5.68%, p < 0.008) EAC than ND-ACL. In the non-surgical limb, D-ACL demonstrated greater hip (D-ACL = 62.59 ± 9.03%, ND-ACL = 25.95 ± 7.15%, p < 0.008), and smaller knee (D-ACL = 13.79 ± 5.57%, ND-ACL = 58.01 ± 7.86%, p < 0.008), EAC than ND-ACL. CONCLUSION: After ACL-R, eccentric loading strategies during a JL task at RTS are different depending upon limb dominance. D-ACL demonstrated a greater knee loading strategy on the surgical side compared to ND-ACL.

Twelve-Week Quadriceps Strength as A Predictor of Quadriceps Strength At Time Of Return To Sport Testing Following Bone-Patellar Tendon-Bone Autograft Anterior Cruciate Ligament Reconstruction.

BACKGROUND: Restoration of quadriceps strength following anterior cruciate ligament reconstruction (ACL-R) continues to challenge both patients and clinicians. Failure to adequately restore quadriceps strength has been linked to decreased patients' self-reported outcomes and an increased risk for re-injury. Early identification of quadriceps strength deficits may assist in tailoring early interventions to better address impairments. PURPOSE: The purpose of this study was to assess the relationship between early (12 weeks following ACL-R) isokinetic peak torque and isokinetic peak torque at time of return to sport (RTS) testing. STUDY DESIGN: Cohort Study. METHODS: A total of 120 participants (males = 55; females =65) were enrolled in the study (age = 16.1±1.4 yrs; height = 1.72±10.5 m; mass = 70.7±16.3 kg). All participants were level 1 or 2 cutting and pivoting sport athletes who underwent a primary bone-patellar tendon-bone autograft ACL-R. Participants were tested at two time points: 12 weeks following surgery and again at time of RTS testing. A linear regression model was carried out to investigate the relationship between age, sex, and isokinetic peak torque at 12 weeks following ACL-R and isokinetic peak torque at time of RTS testing. RESULTS: When 12-week isokinetic peak torque was entered first for the hierarchy regression analysis, this factor was predictive of the peak torque at the time of RTS testing, F(1, 118) = 105.6, p < 0.001, R 2 = 0.472, indicating that the 12-week quadriceps strength accounted for 47% of the variance in the quadriceps strength at the time of RTS testing. When age and sex were added in the regression analysis, both factors only added 0.8% of variance for the quadriceps strength at the time of RTS testing. CONCLUSION: Isokinetic peak torque at 12 weeks following surgery was shown to be a significantly strong predictor (47%) for isokinetic quadriceps strength recovery at time of RTS. This finding underscores the importance of early restoration of quadriceps strength and that while non-modifiable factors such as sex and age are important, early restoration of quadriceps strength most strongly influences late stage quadriceps strength. LEVEL OF EVIDENCE: 3.

Effects of anterior cruciate ligament reconstruction on patellofemoral joint stress and lower extremity biomechanics at 12 weeks post-surgery and at time of return to sport in adolescent females.

BACKGROUND: The purpose of this study was to examine kinematic and kinetic differences associated with patellofemoral pain after anterior cruciate ligament reconstruction between limbs at 12-week post-surgery and at time of return to sport. METHOD: Twenty-four adolescent females completed 5 consecutive single leg squats on each limb at 12-weeks post-surgery and again during their RTS assessment. Peak knee extension moment, peak hip adduction angle, and patellofemoral joint stress at 45 degrees of knee flexion were calculated. Separate two by two repeated measures ANOVA were performed. FINDINGS: There was a significant interaction (limb × time) for knee extension moment (p < 0.001). Surgical limb knee extension moment was significantly less than the non-surgical limb at return to sport (p < 0.001). At 12-weeks the surgical limb was significantly less than non-surgical limb (p < 0.001), additionally the surgical limb was significantly greater at time of return to sport than at 12 weeks (p < 0.001). There was a significant main effect of limb for hip adduction angle (p = 0.002). Surgical limb was significantly greater than non-surgical limb (Surgical = 9.84 (SE 1.53) degree, non-surgical = 4.79 (SE 1.01) degree). There was also a main effect of time and limb for patellofemoral joint stress. Return to sport was significantly greater than 12 weeks and the surgical limb was significantly less than non-surgical limb (Surgical = 4.93 (SE 0325) MPa, Nonsurgical = 5.29 (SE 0.30) MPa). INTERPRETATION: The surgical limb of participants following ACL-R demonstrated variables that have been associated with the development of patellofemoral pain.

Quadriceps strength changes across the continuum of care in adolescent male and female athletes with anterior cruciate ligament injury and reconstruction.

OBJECTIVES: To examine the quadriceps strength (QUADS) on the surgical (SURG) and non-surgical (Non-SURG) limbs in adolescent male and female athletes at pre-operative (PRE), 12 weeks post-operative (12WK), and return to sport (RTS) time points following ACL injury and reconstruction. DESIGN: Prospective cohort study design. SETTING: Clinical Research Laboratory. PARTICIPANTS: 66 adolescent athletes. MAIN OUTCOME MEASURES: Isokinetic QUADS of the SURG and Non-SURG limbs at the PRE, 12WK, and RTS time points were assessed and compared between each time point. RESULTS: Both male and female participants had significantly lower 12 WK QUADS in the SURG limb than the PRE QUADS, but the RTS QUADS was significantly greater than the 12WK QUAD (p < 0.05). However, only female participants had greater RTS QUADS as compared to the PRE QUADS (p < 0.001). For the Non-SURG limb, only male participants had a significant improvement over time (PRE vs RTS; p < 0.001). CONCLUSION: Adolescent males and females differ in their QUADS recovery across the continuum of care following ACLR. Clinicians should consider this pattern of recovery when treating adolescent males and females.

Identification and characterization of flavonoids as sialyltransferase inhibitors.

Sialyltransferases biosynthesize sialyl-glycoconjugates involved in many biological and pathological processes. We investigated and characterized synthetic flavonoid derivatives as sialyltransferase inhibitors. We first examined 54 compounds by solid-phase enzyme assay using beta-galactoside alpha2,6-sialyltransferase 1 (ST6Gal I) and beta-galactoside alpha2,3-sialyltransferase. Several compounds inhibited sialyltransferase enzyme activity regardless of sialyl-linkage reactions. Among them, two compounds showed inhibitory activity against ST6Gal I with IC(50) values less than 10 microM. Three characteristic features of flavonoids were determined by structure-inhibitory activity relationships. First, a double bond between C2-C3 linkages is required for the activity. Second, increasing hydrophilic properties on the B-ring markedly augmented the inhibitory effect. Third, a hydrophobic functional group introduced on the hydroxyl groups of the A-ring enhanced the inhibitory activity. Kinetic analysis using human ST6Gal I indicated a mixed inhibition mechanism of the compounds. In conclusion, the flavonoids identified could be applied for control of cellular expression of sialic acid.

Differential effects of topically applied formalin and aromatic compounds on neurogenic-mediated microvascular leakage in rat skin.

Various volatile organic compounds (VOCs) act as a causative agent of skin inflammation. We investigated the effect of topical application of several VOCs and formalin on microvascular leakage in rat skin. We tested capsaicin, which is a reagent that specifically causes the skin response via endogenously released tachykinins. Evans blue dye extravasation served as an index of the increase in skin vascular permeability. After shaving the abdomen, we applied formalin, m-xylene, toluene, styrene, benzene, ethylbenzene, acetone, diethyl ether, hexane, heptane, cyclohexane and capsaicin to the skin. At 40min after application, skin samples were collected. Among all of the VOCs tested, all of the aromatic compounds significantly produced skin microvascular leakage that was similar to formalin and capsaicin. We also investigated the skin responses seen after the intravenous administration of CP-99,994 (1.5 or 5mg/kg), which is a tachykinin NK1 receptor antagonist, ketotifen (1 or 3mg/kg), which is a histamine H1 receptor antagonist that stabilizes the mast cells, and the topical application of capsazepine (22.5 or 50mM), which is the transient receptor potential vanilloid 1 (TRPV1) antagonist. The response induced by formalin and capsaicin was completely inhibited by CP-99,994. On the other hand, the antagonist partially reduced the response induced by m-xylene, toluene and styrene by 39%, 50% and 46%, respectively. Capsazepine and ketotifen did not alter the response induced by formalin or any of the aromatic compounds. Like capsaicin, formalin and the aromatic compounds at least partially caused skin microvascular leakage, which was due to tachykinin NK1 receptor activation related to the release of tachykinins from the sensory nerve endings. However, it is unlikely that mast cells and TRPV1 play an important role in the skin response.

Knee Loading After ACL-R Is Related to Quadriceps Strength and Knee Extension Differences Across the Continuum of Care.

BACKGROUND: Quadriceps strength and knee extension are believed to be important in the ability to effectively load the knee after anterior cruciate ligament (ACL) reconstruction (ACL-R). PURPOSE: To compare quadriceps strength (QUADS), side-to-side knee extension difference (ExtDiff), and knee energy absorption contribution (EAC) in patients preoperatively, 12 weeks postoperatively, and at return to sport (RTS). A secondary aim was to determine how the factors of QUADS and ExtDiff contributed to the ability to load the knee (knee EAC) at each of the 3 time points. STUDY DESIGN: Case series; Level of evidence, 4. METHODS: Overall, 41 individuals (mean ± SD age, 15.95 ± 1.63 years) were enrolled in this study. QUADS, ExtDiff, and knee EAC during a double-limb squat were collected preoperatively, 12 weeks postoperatively, and at RTS. Isokinetic QUADS was collected at 60 deg/s, normalized to body mass, and averaged across 5 trials. Knee extension was measured with a goniometer, and ExtDiff was calculated for analyses. Knee EAC was measured during double-limb squat descent and was calculated as a percentage of total energy absorption for the limb. Observations were obtained from both the surgical and nonsurgical limbs at the 3 time points. A mixed regression model with random intercept to compare change over the 3 time points was used, and a model selection was conducted with Akaike information criteria. Significance was set at P < .05. RESULTS: Surgical limb QUADS was significantly lower preoperatively (mean ± SD, 1.37 ± 0.49 N·m/kg; P = .0023) and at 12 weeks (1.11 ± 0.38 N·m/kg; P < .0001) than at RTS (1.58 ± 0.47 N·m/kg). Nonsurgical limb QUADS was also significantly lower preoperatively (2.01 ± 0.54 N·m/kg; P < .0256) and at 12 weeks (2.03 ± 0.48 N·m/kg; P < .0233) than at RTS (2.18 ± 0.54 N·m/kg). Knee EAC for the surgical limb was significantly lower at 12 weeks than at RTS (40.98% ± 13.73% vs 47.50% ± 12.04%; P < .0032), and ExtDiff was significantly greater preoperatively than at RTS (-2.68° ± 3.19° vs -0.63° ± 1.43°; P < .0001). Preoperatively, QUADS for both the surgical (P < .0003) and nonsurgical (P = .0023) limbs was a significant predictor of surgical limb knee EAC, explaining 33.99% of the variance. At 12 weeks, surgical limb QUADS was a significant predictor (P < .0051) of surgical limb knee EAC, explaining 18.83% of the variance. At RTS, ExtDiff was a significant predictor (P = .0201) of surgical limb knee EAC, explaining 12.92% of the variance. CONCLUSION: The ability to load the knee after ACL injury changes across the continuum of care and is related to QUADS and ExtDiff. These results provide clinicians with insight into potential contributing factors that may limit knee loading during the rehabilitation process.

Joint-Level Analyses of the Back Squat With and Without Intraset Rest.

Purpose: To provide a joint-level analysis of traditional (TS) and cluster (CS) set structure during the back-squat exercise. Methods: Eight men (24 [3] y, 177.3 [7.9] cm, 82.7 [11.0] kg, 11.9 [3.5] % body fat, and 150.3 [23.0] kg 1-repetition maximum [1RM]) performed the back-squat exercise (80%1RM) using TS (4 × 6, 2-min interset rest) and CS (4 × [2 × 3], 30-s intraset rest, 90-s interset rest), randomly. Lower-limb kinematics were collected by motion capture, as well as kinetic data by bilateral force platforms. Results: CS attenuated the loss in mean power (TS -21.6% [3.9%]; CS -12.4% [7.5%]; P = .042), although no differences in gross movement pattern (sagittal-plane joint angles) within and between conditions were observed (P ≥ .05). However, joint power produced at the hip increased from repetition (REP) 1 through REP 6 during TS, while a decrease was noted at the knee. A similar pattern was observed in the CS condition but was limited to the hip. Joint power produced at the hip increased from REP 1 through REP 3 but returned to REP 1 values before a similar increase through REP 6, resulting in differences between conditions (REP 4, P = .018; REP 5, P = .022). Conclusions: Sagittal-plane joint angles did not change in either condition, although CS elicited greater power. Differing joint power contributions (hip and knee) suggest potential central mechanism that may contribute to enhanced power output during CS and warrant further study. Practitioners should consider incorporating CS into training to promote greater power adaptations and to mitigate fatigue.

Association between double-leg squat and single-leg squat performance and injury incidence among incoming NCAA Division I athletes: A prospective cohort study.

OBJECTIVES: Determine the association between performance on the double-leg squat (DLS) and single-leg squat (SLS) and prospective injury incidence in athletes. DESIGN: Prospective cohort study. SETTING: National Collegiate Athletic Association (NCAA) Division I university. PARTICIPANTS: 111 incoming NCAA Division I athletes from 10 varsity sports teams. MAIN OUTCOME MEASURES: Performance on the DLS and SLS were assessed as "poor" or "non-poor." Lower extremity (LE) injury data for the following year were extracted from electronic medical records. Multivariate Poisson regression was used to compare the incidence of LE injuries in athletes with poor versus non-poor performance on the DLS and SLS. RESULTS: The final models for the DLS and SLS were adjusted for sex and LE injury history and yielded an incidence rate ratio (IRR) of 1.33 (95% CI: 0.80 2.22) for the DLS and 1.62 (95% CI: 0.98, 2.66) for the SLS when comparing poor to non-poor movers. CONCLUSIONS: Athletes with poor LE movement quality assessed on the DLS or SLS had greater incidence of LE injury than those with non-poor movement quality. This is the first study to demonstrate an association between performance on the double-leg squat and single-leg squat and injury risk in NCAA athletes.