RESUMO
BACKGROUND AND PURPOSE: Until now, except thrombolysis, the therapeutical strategies targeting the acute phase of cerebral ischemia have been proven ineffective, and no approach is available to attenuate the delayed cell death mechanisms and the resulting functional deficits in the late phase. Then, we investigated whether a targeted and delayed delivery of pituitary adenylate cyclase-activating polypeptide (PACAP), a peptide known to exert neuroprotective activities, may dampen delayed pathophysiological processes improving functional recovery. METHODS: Three days after permanent focal ischemia, PACAP-producing stem cells were transplanted intracerebro ventricularly in nonimmunosuppressed mice. At 7 and 14 days post ischemia, the effects of this stem cell-based targeted delivery of PACAP on functional recovery, volume lesions, and inflammatory processes were analyzed. RESULTS: The delivery of PACAP in the vicinity of the infarct zone 3 days post stroke promotes fast, stable, and efficient functional recovery. This was correlated with a modulation of the postischemic inflammatory response. Transcriptomic and Ingenuity Pathway Analysis-based bioinformatic analyses identified several gene networks, functions, and key transcriptional factors, such as nuclear factor-κB, C/EBP-ß, and Notch/RBP-J as PACAP's potential targets. Such PACAP-dependent immunomodulation was further confirmed by morphometric and phenotypic analyses of microglial cells showing increased number of Arginase-1(+) cells in mice treated with PACAP-expressing cells specifically, demonstrating the redirection of the microglial response toward a neuroprotective M2 phenotype. CONCLUSIONS: Our results demonstrated that immunomodulatory strategies capable of redirecting the microglial response toward a neuroprotective M2 phenotype in the late phase of brain ischemia could represent attractive options for stroke treatment in a new and unexploited therapeutical window.
Assuntos
Polaridade Celular/fisiologia , Macrófagos/metabolismo , Microglia/metabolismo , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/administração & dosagem , Recuperação de Função Fisiológica/fisiologia , Acidente Vascular Cerebral/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Polaridade Celular/efeitos dos fármacos , Sistemas de Liberação de Medicamentos/métodos , Injeções Intraventriculares , Macrófagos/efeitos dos fármacos , Masculino , Camundongos , Camundongos da Linhagem 129 , Camundongos Transgênicos , Microglia/efeitos dos fármacos , Recuperação de Função Fisiológica/efeitos dos fármacos , Transplante de Células-Tronco/métodos , Acidente Vascular Cerebral/terapia , Fatores de TempoRESUMO
CONTEXT: Neurotensin (NT) modulates corticosteroid secretion from the mammalian adrenal gland. OBJECTIVE: The objective of this study was to investigate the possible involvement of NT in the control of cortisol secretion in the human adrenal gland. DESIGN: In vitro studies were conducted on cultured human adrenocortical cells. SETTING: This study was conducted in a university research laboratory. PATIENTS: Adrenal explants from patients undergoing expanded nephrectomy for kidney cancer were studied. MAIN OUTCOME MEASURE: Cortisol secretion from cultured adrenocortical cells was measured. RESULTS: NT1-11, the N-terminal fragment of NT, dose-dependently inhibited basal and ACTH-stimulated cortisol production by human adrenocortical cells in primary culture. In contrast, NT had no influence on cortisol output at concentrations up to 10(-6) m. HPLC and RT-PCR analyses failed to detect any significant amounts of NT and NT mRNA, respectively, in adrenal extracts. Molecular and pharmacological studies were performed to determine the type of NT receptor involved in the corticostatic effect of NT1-11. RT-PCR analysis revealed the expression of NT receptor type (NTR) 3 mRNA but not NTR1 and NTR2 mRNAs in the human adrenal tissue. However, the pharmacological profile of the adrenal NT1-11 receptor was different from that of NTR3, indicating that this receptor type is not involved in the action of NT1-11 on corticosteroidogenesis. CONCLUSION: Our results indicate that NT1-11 may act as an endocrine factor to inhibit cortisol secretion through activation of a receptor distinct from the classical NTR1, NTR2, and NTR3.
Assuntos
Córtex Suprarrenal/efeitos dos fármacos , Córtex Suprarrenal/metabolismo , Hidrocortisona/metabolismo , Neurotensina/farmacologia , Fragmentos de Peptídeos/farmacologia , Proteínas Adaptadoras de Transporte Vesicular , Córtex Suprarrenal/química , Hormônio Adrenocorticotrópico/farmacologia , Células Cultivadas , Cromatografia Líquida de Alta Pressão , Relação Dose-Resposta a Droga , Humanos , Glicoproteínas de Membrana/genética , Proteínas do Tecido Nervoso/genética , Neurotensina/genética , Fragmentos de Peptídeos/genética , RNA Mensageiro/análise , Receptores de Neurotensina/genética , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
We have previously shown that endothelin (ET)-1 stimulates corticosterone and aldosterone secretion by the frog adrenal gland through activation of ETA receptors positively coupled to both the adenylyl cyclase and phospholipase C (PLC) pathways. The purpose of the present study was to investigate the involvement of calcium in ET-1-induced stimulation of corticosteroid secretion. Cytoautoradiographic labeling using [125I]ET-1 as a tracer revealed the presence of ET-1 binding sites on adrenocortical cells. Administration of graded concentrations of ET-1 in the vicinity of adrenocortical cells provoked a dose-dependent increase in cytosolic calcium concentrations ([Ca2+]i). ET-1 induced a biphasic response consisting of an immediate and transient peak of [Ca2+]i followed by a plateau phase. Preincubation of the cells with the calcium-ATPase inhibitor thapsigargin or the PLC inhibitor U-73122 reduced the amplitude of the transient phase. Administration of the calcium chelator EGTA or the protein kinase A inhibitor H-89 attenuated the plateau phase. The [Ca2+]i response to ET-1 was markedly reduced during concomitant administration of U-73122 and H-89. Preincubation of the cells with the L-type calcium channel blocker nifedipine attenuated the plateau phase. Corticosteroid secretion from perifused frog adrenal slices was almost completely suppressed by thapsigargin and reduced by nifedipine. Taken together, these data indicate that activation of ETA receptors in frog adrenocortical cells provokes immediate stimulation of PLC, which causes an early mobilization of calcium from intracellular stores, and activates adenylyl cyclase, which results in delayed calcium influx through L-type calcium channels. The resulting increase in [Ca2+]i plays a pivotal role in ET-1-induced corticosteroid secretion.
Assuntos
Córtex Suprarrenal/metabolismo , Canais de Cálcio Tipo L/metabolismo , Cálcio/metabolismo , Membranas Intracelulares/metabolismo , Receptor de Endotelina A/fisiologia , Córtex Suprarrenal/citologia , Córtex Suprarrenal/efeitos dos fármacos , Animais , Sítios de Ligação , Transporte Biológico , Células Cultivadas , Corticosterona/metabolismo , Endotelina-1/metabolismo , Endotelina-1/farmacologia , Masculino , Concentração Osmolar , Rana esculenta , Distribuição TecidualRESUMO
Beyond their motor impairments, the cerebellar Lurcher mutant mice show an alteration of the anxiety-related behaviors we called "behavioral disinhibition". This is characterized by a low avoidance towards the open arms of the elevated plus-maze device paradoxically combined with a dramatic blood corticosterone level rise induced by the exposure to the experimental conditions. The present study was aimed at determining if the disinhibition of the mutants could be caused by their stress-induced high corticosterone rate. For this purpose, we compared the behaviors of Lurcher and control mice in the elevated plus-maze test after injection of either 2-methyl-1.2-di-3-pyridil-1-propanone (metyrapone; 75 mg/kg), a corticosterone synthesis inhibitor, or vehicle alone (Tween 80, 5%). Our results showed that metyrapone, although efficiently reducing their blood corticosterone rate, provoked only modest modifications of the anxiety-related behaviors in mice of both genotypes. As a result, the behavioral distance between the Lurcher and control mice slightly decreased, without being totally abolished. Thus, it seems that the behavioral disinhibition of the mutants is caused only in part by their stress-provoked high corticosterone level. As a complementary hypothesis, we propose that the behavioral disturbances observed in the Lurcher mice also might arise from dysfunctions of the neural pathways connecting the cerebellum with some limbic structures known to be highly involved in the regulation of emotions.