Photobiomodulation therapy improves human dental pulp stem cell viability and migration in vitro associated to upregulation of histone acetylation.

This in vitro study evaluated the role of photobiomodulation therapy (PBMT) on viability and migration of human dental pulp stem cells (hDPSCs) and its association to epigenetic mechanisms such as histone acetylation. The hDPSCs were characterized and assigned into control and PBMT groups. For the PBMT, five laser irradiations at 6-h intervals were performed using a continuous-wave InGaAlP diode laser. Viability (MTT), migration (scratch), and histone acetylation H3 (H3K9ac immunofluorescence) were evaluated immediately after the last irradiation. PBMT significantly increased the viability (P = 0.004). Also, PBMT group showed significantly increased migration of cells in the wound compared to the control in 6 h (P = 0.002), 12 h (P = 0.014) and 18 h (P = 0.083) being faster than the control, which only finished the process at 24 h. PBMT induced epigenetic modifications in hDPSC due to increased histone acetylation (P = 0.001). PBMT increased viability and migration of hDPSCs, which are related with the upregulation of histone acetylation and could be considered a promising adjuvant therapy for regenerative endodontic treatment.

Periodontal disease as a risk indicator for poor physical fitness: a cross-sectional observational study.

BACKGROUND: Physical inactivity has been associated with poor oral health. The aim of this study is to assess whether periodontal disease is a risk indicator for poor physical fitness. METHODS: This cross-sectional study included 111 males who performed a physical fitness test (PFT) composed of four exercises: 1) push-ups conducted by pushing the body up and lowering it down using the arms; 2) pull-ups with the body suspended by the arms gripped on a bar; 3) sit-ups in which the upper and lower vertebrae are lifted from the floor; and 4) running for 12 minutes. A PFT score (range of 1 to 300) was determined for each participant, with higher scores indicating better physical fitness. One periodontist assessed attachment loss (AL) and probing depth (PD). Physical fitness was dichotomized according to whether the highest PFT score was "achieved" or "not achieved." Multivariable logistic models were fitted adjusting for age, overweight (body mass index of 25 to 29.9 kg/m(2)), and frequency of daily exercise. RESULTS: The mean age of the sample was 34.8 ± 10.3 years. Overweight individuals demonstrated significantly lower PFT scores (276.9 ± 24.1 points) than normal-weight individuals (289.3 ± 16.8 points). Individuals presenting at least one tooth with AL ≥ 4 mm had significantly lower PFT scores (277.8 ± 23.6 points) compared with those without this status (285.9 ± 20.2 points). A 1-mm increment in PD or AL significantly decreased the chance of reaching the highest PFT score by 69% or 75%, respectively. CONCLUSION: Periodontal disease may be considered a risk indicator for poor physical fitness in males.