RESUMO
Association studies have identified dozens of genetic variants linked to training responses and sport-related traits. However, no intervention studies utilizing the idea of personalised training based on athlete's genetic profile have been conducted. Here we propose an algorithm that allows achieving greater results in response to high- or low-intensity resistance training programs by predicting athlete's potential for the development of power and endurance qualities with the panel of 15 performance-associated gene polymorphisms. To develop and validate such an algorithm we performed two studies in independent cohorts of male athletes (study 1: athletes from different sports (n = 28); study 2: soccer players (n = 39)). In both studies athletes completed an eight-week high- or low-intensity resistance training program, which either matched or mismatched their individual genotype. Two variables of explosive power and aerobic fitness, as measured by the countermovement jump (CMJ) and aerobic 3-min cycle test (Aero3) were assessed pre and post 8 weeks of resistance training. In study 1, the athletes from the matched groups (i.e. high-intensity trained with power genotype or low-intensity trained with endurance genotype) significantly increased results in CMJ (P = 0.0005) and Aero3 (P = 0.0004). Whereas, athletes from the mismatched group (i.e. high-intensity trained with endurance genotype or low-intensity trained with power genotype) demonstrated non-significant improvements in CMJ (P = 0.175) and less prominent results in Aero3 (P = 0.0134). In study 2, soccer players from the matched group also demonstrated significantly greater (P < 0.0001) performance changes in both tests compared to the mismatched group. Among non- or low responders of both studies, 82% of athletes (both for CMJ and Aero3) were from the mismatched group (P < 0.0001). Our results indicate that matching the individual's genotype with the appropriate training modality leads to more effective resistance training. The developed algorithm may be used to guide individualised resistance-training interventions.
RESUMO
Risk variants of fat mass and obesity-associated (FTO) gene have been associated with increased obesity. However, the evidence for associations between FTO genotype and macronutrient intake has not been reviewed systematically. Our aim was to evaluate the potential associations between FTO genotype and intakes of total energy, fat, carbohydrate and protein. We undertook a systematic literature search in OVID MEDLINE, Scopus, EMBASE and Cochrane of associations between macronutrient intake and FTO genotype in adults. Beta coefficients and confidence intervals (CIs) were used for per allele comparisons. Random-effect models assessed the pooled effect sizes. We identified 56 eligible studies reporting on 213,173 adults. For each copy of the FTO risk allele, individuals reported 6.46 kcal day(-1) (95% CI: 10.76, 2.16) lower total energy intake (P = 0.003). Total fat (P = 0.028) and protein (P = 0.006), but not carbohydrate intakes, were higher in those carrying the FTO risk allele. After adjustment for body weight, total energy intakes remained significantly lower in individuals with the FTO risk genotype (P = 0.028). The FTO risk allele is associated with a lower reported total energy intake and with altered patterns of macronutrient intake. Although significant, these differences are small and further research is needed to determine whether the associations are independent of dietary misreporting.
Assuntos
Obesidade/genética , Polimorfismo de Nucleotídeo Único/genética , Proteínas/genética , Adulto , Dioxigenase FTO Dependente de alfa-Cetoglutarato , Carboidratos da Dieta/metabolismo , Gorduras na Dieta/metabolismo , Ingestão de Energia , Interação Gene-Ambiente , Predisposição Genética para Doença , Estudo de Associação Genômica Ampla , Genótipo , Humanos , Obesidade/epidemiologia , Estudos Observacionais como Assunto , Fatores de RiscoRESUMO
More than 80% of patients with testicular germ cell tumours (TGCT) are cured using cisplatin-based combination chemotherapy, and resistance to cisplatin is the final barrier to the cure of nearly all patients with this disease. In this study, we used quantitative polymerase chain reaction (Q-PCR) to investigate the role of DNA repair in cisplatin resistance in two genes, one transcribed and one not transcribed. Three pairs of cisplatin-sensitive and resistant cell lines were used, two derived from TGCT and one from a bladder cancer. In these pairs of sublines, we observed no major differences between the repair of cisplatin-induced damage in the transcribed and nontranscribed genes, nor did there appear to be any relationship between DNA repair capacity and the development of cisplatin resistance. Despite the strong indication that the sensitivity of testis tumour cells to cisplatin is related to their reduced ability to repair cisplatin-damaged DNA, these cells apparently do not become resistant to cisplatin by acquiring DNA repair capacity.
Assuntos
Antineoplásicos/farmacologia , Cisplatino/farmacologia , Reparo do DNA , Reação em Cadeia da Polimerase , Cisplatino/farmacocinética , Resistência a Medicamentos , Humanos , Células Tumorais CultivadasRESUMO
Very-low-carbohydrate diets or ketogenic diets have been in use since the 1920s as a therapy for epilepsy and can, in some cases, completely remove the need for medication. From the 1960s onwards they have become widely known as one of the most common methods for obesity treatment. Recent work over the last decade or so has provided evidence of the therapeutic potential of ketogenic diets in many pathological conditions, such as diabetes, polycystic ovary syndrome, acne, neurological diseases, cancer and the amelioration of respiratory and cardiovascular disease risk factors. The possibility that modifying food intake can be useful for reducing or eliminating pharmaceutical methods of treatment, which are often lifelong with significant side effects, calls for serious investigation. This review revisits the meaning of physiological ketosis in the light of this evidence and considers possible mechanisms for the therapeutic actions of the ketogenic diet on different diseases. The present review also questions whether there are still some preconceived ideas about ketogenic diets, which may be presenting unnecessary barriers to their use as therapeutic tools in the physician's hand.
Assuntos
Dieta com Restrição de Carboidratos , Dieta Cetogênica , Cetose , Doenças Metabólicas/dietoterapia , Neoplasias/dietoterapia , Doenças do Sistema Nervoso/dietoterapia , Redução de Peso/fisiologia , Doenças Cardiovasculares/dietoterapia , Doenças Cardiovasculares/metabolismo , Dieta Redutora , Carboidratos da Dieta/administração & dosagem , Glucose/metabolismo , Humanos , Doenças Metabólicas/metabolismo , Doenças do Sistema Nervoso/metabolismo , Obesidade/dietoterapia , Obesidade/metabolismoRESUMO
Obesity is reaching epidemic proportions in Western countries and is a strong risk factor for cardiovascular disease. Despite the constant recommendations of health care organizations regarding the importance of weight control, this goal often fails. Although there is a common agreement about the concept that exercise and diet are two key factors for the control of body weight, the ideal amount and type of exercise and also the ideal diet for weight control are still under debate. A widely accepted nutritional regime is the Mediterranean diet that has evident health benefits although less attention has been paid to see if the effects are due to other lifestyle factors which may contribute to the health benefits perhaps as much as specific food choices. There are several other options available to the physician that may produce good weight loss results in the short/medium term and also for maintenance of the goal achieved. One of these strategies is the ketogenic diet or VLCKD (very low carbohydrate ketogenic diet) that has been widely studied in recent years. Most studies show that this diet has a solid physiological and biochemical basis which is able to induce effective weight loss and improvement of several parameters of cardiovascular risk. This review discusses the physiological basis of VLCKD and the main applications together with its strengths and weaknesses compared to common dietary recommendations.
Assuntos
Dieta com Restrição de Carboidratos , Dieta Cetogênica , Dieta Redutora , Obesidade/dietoterapia , Tecido Adiposo/metabolismo , Doenças Cardiovasculares/prevenção & controle , Dieta com Restrição de Carboidratos/efeitos adversos , Dieta Cetogênica/efeitos adversos , Dieta Mediterrânea , Dieta Redutora/efeitos adversos , Carboidratos da Dieta/metabolismo , Metabolismo Energético , Humanos , Hidroximetilglutaril-CoA Redutases/metabolismo , Corpos Cetônicos/metabolismo , Obesidade/epidemiologia , Obesidade/metabolismo , Fatores de Risco , Redução de PesoAssuntos
Adutos de DNA/análise , Reação em Cadeia da Polimerase/métodos , Antineoplásicos/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Sequência de Bases , Sítios de Ligação , DNA/química , DNA/efeitos dos fármacos , DNA/genética , DNA/metabolismo , Dano ao DNA , Primers do DNA/genética , DNA de Neoplasias/química , DNA de Neoplasias/efeitos dos fármacos , DNA de Neoplasias/genética , DNA de Neoplasias/metabolismo , Humanos , Técnicas In VitroAssuntos
Dano ao DNA , Reparo do DNA , DNA/genética , Genes ras , Reação em Cadeia da Polimerase/métodos , Animais , Sequência de Bases , Biotinilação , Adesão Celular , Células Cultivadas , Cisplatino/toxicidade , DNA/efeitos dos fármacos , DNA/isolamento & purificação , DNA de Cadeia Simples/genética , DNA de Cadeia Simples/isolamento & purificação , Eletroforese em Gel de Ágar/métodos , Humanos , Indicadores e Reagentes , Íntrons , Dados de Sequência Molecular , Mutagênese , Mutagênicos/toxicidadeAssuntos
Dano ao DNA , Reparo do DNA , DNA/isolamento & purificação , Genes ras , Reação em Cadeia da Polimerase/métodos , Animais , Sequência de Bases , Biotinilação , Adesão Celular , Células Cultivadas , DNA/efeitos dos fármacos , DNA/efeitos da radiação , Primers do DNA , DNA de Cadeia Simples/genética , Eletroforese em Gel de Poliacrilamida/métodos , Humanos , Indicadores e Reagentes , Íntrons , Dados de Sequência Molecular , Mutagênese , Mutagênicos/toxicidade , Regiões Promotoras Genéticas , Taq Polimerase , Raios UltravioletaAssuntos
Grânulos Citoplasmáticos/metabolismo , Ilhotas Pancreáticas/metabolismo , Adenosina Trifosfatases/fisiologia , Animais , Carboxipeptidase H , Carboxipeptidases/biossíntese , Cromogranina A , Cromograninas/biossíntese , Grânulos Citoplasmáticos/análise , Humanos , Insulina/metabolismo , Secreção de Insulina , Ilhotas Pancreáticas/ultraestrutura , Proteínas de Membrana/biossíntese , Proinsulina/metabolismo , Prótons , RatosRESUMO
Monoclonal antibodies to insulin secretory granule membranes were obtained following immunization of mice with granule membranes purified from a rat transplantable insulinoma. The specificities of the antibodies were investigated by using binding assays with different insulinoma subcellular fractions, by indirect immunofluorescence studies with intact and permeabilized cells, and by immunoblotting of granule membrane proteins fractionated by SDS/polyacrylamide-gel electrophoresis. Fifty-six antibodies were characterized initially, and 21 representative cell lines were cloned. The antibodies fell into four categories: (1) binding preferentially to secretory granules, and reacting with a component of approx. 80,000 Da on immunoblots (antigen designated SGM 80); (2) binding preferentially to secretory granules, and reacting with components of approx. 110,000 and 50,000 Da on immunoblots (antigen designated SGM 110); (3) binding preferentially to secretory granules but unreactive on immunoblots; (4) binding to membrane antigen(s) with a widespread intracellular distribution which included granules and plasma membranes. The antigens SGM 80 and SGM 110 were studied in more detail and both were shown to be integral membrane glycoproteins with antigenic determinants located on the internal face of the secretory granule membrane. These antigens were also present in normal rat islets of Langerhans and similar components were detected by immunoblotting in secretory granules from anterior pituitary and adrenal medulla. Proteins which were immunologically related to SGM 80 and SGM 110, but distinct in molecular size, were also identified in liver. It is concluded that secretory granules contain specific components which are restricted in subcellular location but widespread in tissue distribution. The antibodies obtained will be valuable reagents in the further investigation of the biogenesis and turnover of insulin secretory granules.
Assuntos
Adenoma de Células das Ilhotas Pancreáticas/imunologia , Anticorpos Monoclonais/isolamento & purificação , Grânulos Citoplasmáticos/imunologia , Insulina/metabolismo , Insulinoma/imunologia , Neoplasias Pancreáticas/imunologia , Animais , Especificidade de Anticorpos , Antígenos de Neoplasias/análise , Sítios de Ligação , Eletroforese em Gel de Poliacrilamida , Epitopos/análise , Glicosilação , Imunoeletroforese , Secreção de Insulina , Glicoproteínas de Membrana/imunologia , Ratos , Frações Subcelulares/imunologiaRESUMO
The biosynthesis of a component SGM 110, specifically localized to the membrane of insulin secretory granules, was studied in rat insulinoma cells and in normal islets of Langerhans. Cells or islets were labelled with [35S]methionine or [3H]mannose and SGM 110 was immunoprecipitated by using a monoclonal antibody. Pulse-chase experiments demonstrated that the nascent polypeptide was cotranslationally glycosylated to form a 97,000 Da peptide which in turn was processed to the mature 110,000 Da form. A 50,000 Da form detected by immunoblotting with the same antibody was not conspicuously labelled even after a 20 h chase incubation, suggesting that it represented late processing of SGM 110 in lysosomes. With insulinoma cells, an increase in medium glucose concentration from 3 mM to 20 mM was without effect on the secretion of insulin or on the biosynthesis of (pro)insulin or SGM 110. In normal islets, however, 20 mM-glucose produced a 17-fold increase in (pro)insulin biosynthesis and a 13-fold increase in SGM 110 biosynthesis, compared with only a 2-fold increase in total protein synthesis, as judged by incorporation of [35S]methionine during a 1 h incubation. The effect of glucose on both (pro)insulin and SGM 110 biosynthesis was blocked by the addition of mannoheptulose, but not by the removal of extracellular calcium, both of which conditions inhibit insulin secretion. In contrast tolbutamide, an agent which stimulates insulin secretion, did not enhance the biosynthesis of (pro)insulin or SGM 110. It is concluded that at least one protein component of the insulin secretory granule membrane is synthesized co-ordinately with proinsulin and is subject to similar regulatory mechanisms. Factors which acutely control insulin secretion may also control granule biogenesis, although the two processes are not coupled in an obligatory fashion.
Assuntos
Adenoma de Células das Ilhotas Pancreáticas/metabolismo , Grânulos Citoplasmáticos/metabolismo , Insulina/metabolismo , Insulinoma/metabolismo , Glicoproteínas de Membrana/biossíntese , Neoplasias Pancreáticas/metabolismo , Animais , Cálcio/farmacologia , Precipitação Química , Glucose/farmacologia , Imunoeletroforese , Técnicas In Vitro , Secreção de Insulina , Manoeptulose/farmacologia , Proinsulina/biossíntese , Ratos , Tolbutamida/farmacologiaRESUMO
A new PCR based technique has been developed to investigate the sequence selectivity of adduct formation by DNA damaging agents in a single copy gene in isolated genomic DNA or in drug treated cells. Single-strand ligation PCR (sslig-PCR) demonstrated that cisplatin and nitrogen mustards reacted with guanine in an N-ras fragment with varying sequence specificity similar to that observed previously in plasmid DNA. In cisplatin-treated cells sslig-PCR demonstrated all the adducts found in isolated DNA and with the same sequence selectivity showing a preference for GG and AG sites. However, in cells an additional site of DNA binding of cisplatin was observed at the two occurrences of the sequence 5'-TACT-3' on the transcribed and non-transcribed strands. This sequence is not a recognised target for cisplatin and represents a novel adduct formed in cells.
Assuntos
Antineoplásicos/farmacologia , Cisplatino/farmacologia , Dano ao DNA , Reação em Cadeia da Polimerase/métodos , Sequência de Bases , Sítios de Ligação , Linhagem Celular , DNA , Mecloretamina/farmacologia , Dados de Sequência Molecular , Transcrição GênicaRESUMO
The quantitative polymerase chain reaction (QPCR)-based assay was used to measure DNA damage and repair to a small (523 bp) fragment of the single-copy human N-ras gene in K562 cells. Compared with previous methods DNA preparation from treated cells and the subsequent detection of the radioactive product were considerably simplified. The results demonstrated that QPCR can be used to measure damage in a small gene segment, caused by cisplatin, nitrogen, and quinacrine mustards. Drug-DNA adducts produced by two novel minor groove binding, sequence-specific molecules (AT-486 and DSB-120) could be detected at physiologically relevant concentrations of drug. For both cisplatin and nitrogen mustard the concentrations required to cause damage in cells were higher than those needed to cause equivalent damage in isolated DNA. In contrast both AT-486 and quinacrine mustard caused more damage at equimolar concentrations in cells than in isolated DNA. DSB-120, which is closely related to AT-486, was found to be 15-fold less effective than the latter at causing damage in treated cells despite similar reactivity with isolated DNA. Repair of damage caused by quinacrine mustard to the same small gene fragment was found to proceed at a constant rate over 24 h. The QPCR assay presented here is a simple quantitative method to measure damage and repair in subgene functional units such as promoters, introns, and exons.
Assuntos
Antineoplásicos/toxicidade , Dano ao DNA , Reparo do DNA , Reação em Cadeia da Polimerase , Sequência de Bases , Primers do DNA , Humanos , Dados de Sequência Molecular , Células Tumorais CultivadasRESUMO
The biosynthesis of insulin and betagranin, a 20-21 kDa co-secreted chromogranin A-related protein, were investigated in isolated insulinoma cells and islets. The insulinoma tissue processed proinsulin to insulin with kinetics similar to those reported in islet tissue. Unlike islets, however, the insulinoma released almost one-quarter of the newly synthesized proinsulin into the medium 10-40 min after its formation. Betagranin was initially immunoprecipitated as a 100 kDa precursor form, which was indistinguishable from chromogranin A in size and immunoreactivity and by peptide mapping. After an initial lag of 10-20 min, the precursor was converted progressively into betagranin, which appeared to be a stable end product. Formation of betagranin and insulin from their respective precursors followed a parallel course and could likewise be inhibited by NH4+, chloroquine and monensin, added either before labelling or at any point of time up to 15 min after labelling. As with proinsulin, approximately one-quarter of the betagranin precursor was released 10-40 min after synthesis. It is concluded that betagranin is produced by limited proteolysis from a chromogranin A precursor in pancreatic beta-cells by a cellular pathway indistinguishable from that of insulin from proinsulin. Chromogranin A is highly conserved in the N-terminal region represented by betagranin, further suggesting that the biological activity of chromogranin A may reside in a derived peptide rather than in the parent molecule.
Assuntos
Cromograninas/biossíntese , Ilhotas Pancreáticas/metabolismo , Proteínas do Tecido Nervoso/biossíntese , Animais , Precipitação Química , Cloroquina/farmacologia , Cromogranina A , Cromograninas/imunologia , Cromograninas/metabolismo , Eletroforese em Gel de Poliacrilamida , Técnicas In Vitro , Insulina/imunologia , Insulinoma/metabolismo , Ilhotas Pancreáticas/efeitos dos fármacos , Neoplasias Pancreáticas/metabolismo , Proinsulina/biossíntese , RatosRESUMO
The levels of N-alkyl purine and DNA interstrand crosslink formation, produced by the clinically used nitrogen mustard antitumour drug mechlorethamine (HN2), were quantitated at the level of specific genes in a panel of human tumour cell lines using modified Southern blotting methods. When purified genomic DNA was treated with HN2 in vitro, no significant difference in the extent of N-alkyl purine or interstrand crosslink formation in the N-ras, c-myc or CD3delta genes was observed. When the cell lines LS174T, Colo320HSR, J6 and U937 were treated with HN2, however, there was significant heterogeneity in the levels of N-alkyl purine formation in the three genes. The rank order of the extent of damage in the three genes was also different in the cell lines. The level of alkylation did not correlate with either the transcriptional activity of a gene or drug sensitivity. Crosslinks were not detectable in the N-ras or c-myc genes of LS174T, J6 or U937 cells treated with HN2, and only detectable in the amplified c-myc gene of the Colo320HSR cell line. In the related cell line Colo320DM, which has both native and translocated c-myc alleles which are both amplified and episomal, crosslinks were detected in the amplified native and rearranged c-myc alleles, and also in the N-ras gene which is also amplified in this cell line. For bifunctional alkylating agents such as HN2, therefore, heterogeneity of DNA damage can occur between different genes in human cells and can also vary for different lesions produced by the same agent. In addition, this heterogeneity can differ between human tumour cell lines.
Assuntos
Antineoplásicos Alquilantes/farmacologia , DNA/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Mecloretamina/farmacologia , Alquilação/efeitos dos fármacos , Reagentes de Ligações Cruzadas , DNA de Neoplasias/efeitos dos fármacos , Humanos , Neoplasias/tratamento farmacológico , Neoplasias/genética , Células Tumorais CultivadasRESUMO
Metastatic testicular germ cell tumours can be cured using cisplatin-based combination chemotherapy. To investigate the role of DNA repair in cisplatin sensitivity, we measured the formation and removal of cisplatin adducts in the whole genome and in specific genomic regions in 3 testis and 3 bladder cancer cell lines. Following a 1 hr exposure to 50 microM cisplatin, the mean level of DNA platination was lower in the testis tumour cell lines. During a 72 hr post-treatment incubation period, the 3 bladder cell lines removed platinum from the overall genome, whereas 2 of the testis tumour cell lines showed relatively little reduction of DNA platination. The third testis tumour cell line, SuSa, showed an intermediate capacity to remove cisplatin. Cisplatin-induced damage and repair in selected regions of the actively transcribed N-ras gene and the inactive CD3delta gene were measured using quantitative PCR. The data were in agreement with those obtained with atomic absorption spectroscopy for the whole genome, showing that the bladder lines were repair-proficient: 2 of the testis tumour cell lines showed no repair, and the third testis line, SuSa, showed an intermediate level of repair in these 2 genes. Our findings confirm that reduced capacity to repair cisplatin-damaged DNA may contribute to the hypersensitivity of testis tumour cells to DNA-damaging agents.