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1.
Eur J Clin Nutr ; 74(1): 209, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31624362

RESUMO

Since publication of the original paper, the authors realised that the units of measurement in Table 1 were incorrect. These were changed from "(mg/l)" to "(% dose excreted)". Furthermore a minor typo in the title of the article was also corrected. These changes are now present in the HTML and PDF versions of the paper.

2.
Eur J Clin Nutr ; 72(8): 1180-1182, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29872160

RESUMO

Sodium intake is assessed using 24 h urinary excretion; it is important to ensure urine collections are complete. This can be validated by monitoring urinary excretion of p-aminobenzoic acid (PABA) administered in tablet form at intervals during the urine collection. Unavoidable change of PABA tablet supplier and analytical procedure required re-establishment of the thresholds consistent with a complete collection. Reference ranges for adults without reported intestinal or renal disease were determined by HPLC (70-103%) and colorimetry (84-120%). Some individuals excreted a small, measurable amount of PABA the following day but this did not represent the balance of the PABA ingested. Assay of the PABA tablets confirmed the stated dose (80 mg) and demonstrated their stability up to 8 years (duration of study) at room temperature. These tablets have been used and the reference ranges applied in UK national population surveys since 2008.


Assuntos
Inquéritos sobre Dietas/métodos , Inquéritos Nutricionais/métodos , Coleta de Urina/métodos , Ácido 4-Aminobenzoico/urina , Adulto , Cromatografia Líquida de Alta Pressão , Colorimetria , Estabilidade de Medicamentos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Reino Unido , Coleta de Urina/normas
3.
J AOAC Int ; 100(5): 1288-1293, 2017 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-28797319

RESUMO

The Vitamin D Standardization Program (VDSP) coordinated a study in 2012 to assess the commutability of reference materials and proficiency testing/external quality assurance materials for total 25-hydroxyvitamin D [25(OH)D] in human serum, the primary indicator of vitamin D status. A set of 50 single-donor serum samples as well as 17 reference and proficiency testing/external quality assessment materials were analyzed by participating laboratories that used either immunoassay or LC-MS methods for total 25(OH)D. The commutability test materials included National Institute of Standards and Technology Standard Reference Material 972a Vitamin D Metabolites in Human Serum as well as materials from the College of American Pathologists and the Vitamin D External Quality Assessment Scheme. Study protocols and data analysis procedures were in accordance with Clinical and Laboratory Standards Institute guidelines. The majority of the test materials were found to be commutable with the methods used in this commutability study. These results provide guidance for laboratories needing to choose appropriate reference materials and select proficiency or external quality assessment programs and will serve as a foundation for additional VDSP studies.


Assuntos
Análise Química do Sangue/normas , Ensaio de Proficiência Laboratorial , Vitamina D/análogos & derivados , Humanos , Controle de Qualidade , Padrões de Referência , Estados Unidos , Vitamina D/sangue
4.
J AOAC Int ; 100(5): 1244-1252, 2017 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-28822355

RESUMO

The Vitamin D Standardization Program (VDSP) coordinated an interlaboratory study to assess the comparability of measurements of total 25-hydroxyvitamin D [25(OH)D] in human serum, which is the primary marker of vitamin D status. A set of 50 individual donor samples were analyzed by 15 different laboratories representing national nutrition surveys, assay manufacturers, and clinical and/or research laboratories to provide results for total 25(OH)D using both immunoassays (IAs) and LC tandem MS (MS/MS). The results were evaluated relative to bias compared with the target values assigned based on a combination of measurements at Ghent University (Belgium) and the U.S. National Institute of Standards and Technology using reference measurement procedures for the determination of 25(OH)D2 and 25(OH)D3. CV and mean bias for each laboratory and assay platform were assessed and compared with previously established VDSP performance criteria, namely CV ≤ 10% and mean bias ≤ 5%. Nearly all LC-MS/MS results achieved VDSP criteria, whereas only 50% of IAs met the criterion for a ≤10% CV and only three of eight IAs achieved the ≤5% bias. These results establish a benchmark for the evaluation of 25(OH)D assay performance and standardization activities in the future.


Assuntos
Análise Química do Sangue/normas , Vitamina D/análogos & derivados , Cromatografia Líquida/normas , Humanos , Imunoensaio/normas , Padrões de Referência , Espectrometria de Massas em Tandem/normas , Vitamina D/sangue
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