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Ion-selective membrane has broad application in various fields, while the present solution-processed techniques can only prepare uniform membrane with microscale thickness. Herein, a high-quality polymer membrane with nanoscale thickness and uniformity is precisely prepared by controlling solution spreading and solvent evaporation stability/rate. With the arrayed capillaries, the stable spreading of polymer solution with volume of microliter induces the formation of solution film with micrometers thickness. Moreover, the fast increase of solution dynamic viscosity during solvent evaporation inhibits nonuniform Marangoni flow and capillary flow in solution film. Consequently, the uniform Nafion-Li membranes with â¼200 nm thickness are prepared, while their Li+ conductivity is 2 orders of magnitude higher than that of commercially Nafion-117 membrane. Taking lithium-sulfur battery as a model device, the cells (capacities of 8-10 mAh cm-2) can stably operate for 150 cycles at a S loading of 12 mg cm-2 and an electrolyte/sulfur ratio of â¼7.
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Metal electrode with long cycle life is decisive for the actual use of metal rechargeable batteries, while the dendrite growth and side reaction limit their cyclic stability. Herein, the construction of polymer and inorganic-rich SEI tandem layer structure on Li metal can be used for extraordinarily extending its cycle life is reported, which is generated by an in situ PVDF/LiF/LiNO3 (PLL) gel layer on the surface of Li metal with a chemically compatible ether solvent. The cycle life of Li//Li cells with the tandem layer structure is over 6000 h, six times longer than those with LiNO3 homogeneous electrolyte. It highlights the importance of LiNO3 concentration gradient electrolyte formed by the in situ PLL gel layer, in which highly concentrated LiNO3 is confined on the surface of Li metal to generate the uniform and inorganic-rich LiF/Li2O/Li3N layer on the bottom of PVDF/LiF with good mechanical strength, resulting in the dendrite free anode in cell cycling. The assembled Li//LiFePO4 and Li//NMC811 batteries show the capacity retention rate of 80.9% after 800 cycles and 82.3% after 500 cycles, respectively, much higher than those of references.
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Copper/zinc superoxide dismutase (Cu/Zn-SOD) can effectively eliminate reactive oxygen species (ROS)ï¼avoid damage from O2 to the body, and maintain O2 balance. In this study, multi-step high-performance liquid chromatography (HPLC), combined with Mass Spectrometry (MS), was used to isolate and identify Cu/Zn-SOD from the serum of Pinctada fucata martensii (P. f. martensii) and was designated as PmECSOD. With a length of 1864 bp and an open reading frame (ORF) of 1422 bp, the cDNA encodes a 473 amino acid protein. The PmECSOD transcript was detected in multiple tissues by quantitative real-time PCR (qRT-PCR), with its highest expression level being in the gills. Additionally, the temporal expression of PmECSOD mRNA in the hemolymph was highest at 48 h after in vivo stimulation with Escherichia coli and Micrococcus luteus. The results from this study provide a valuable base for further exploration of molluscan innate immunity and immune response.
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Sequência de Aminoácidos , Imunidade Inata , Filogenia , Pinctada , Superóxido Dismutase , Animais , Pinctada/imunologia , Pinctada/genética , Pinctada/enzimologia , Superóxido Dismutase/genética , Superóxido Dismutase/química , Superóxido Dismutase/metabolismo , Superóxido Dismutase/imunologia , Imunidade Inata/genética , Perfilação da Expressão Gênica/veterinária , Sequência de Bases , Alinhamento de Sequência/veterinária , Escherichia coli , DNA Complementar/genética , Micrococcus luteus/fisiologia , Regulação da Expressão Gênica/imunologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
Cowpea or black-eyed pea [Vigna unguiculata (L.) Walp.] is a dual-purpose leguminous crop grown for food and fodder. In September 2022, cowpea plants exhibiting symptoms of a leaf spot and blight were observed in Renda Town located in Jingning County of Gansu Province, China, with the disease incidence in individual cowpea fields as high as 100%. Diseased leaves showed variable-sized, nearly circular brown blotches, large blotches with dark brown margins, and the adaxial surfaces of blotches had small black dots and whorls (Fig. 1). Multiple isolates with consistent colony characteristics were obtained from cowpea leaves with typical symptoms. The isolates were transferred to fresh potato dextrose agar medium (PDA) and then purified by transferring hyphal tips to PDA. Three isolates, JNJD-1, JNJD-2, and JNJD-3, were selected for subsequent identification and pathogenicity determination. After eight days at 25â on PDA, the colonies appeared irregular, aerial mycelium dense, cottony, atrovirens to olive brown, with white hyphae on the undulate margin (Fig. 2A and B). The pycnidia were globose to sub-globose, brown to dark brown, with 70-110 µm diameters. Single celled hyaline conidia were ellipsoidal to oblong with obtuse ends, and measured 6.6-9.3 × 2.8-4.1 µm (xÌ = 7.8 × 3.5 µm, n = 50) (Fig. 2C). Morphological characteristics are similar to the description of the genus Boeremia (Aveskamp et al, 2010). Primer pairs ITS1/ITS4, LR0R/LR5, fRPB2-5F2/fRPB2-7cR, and TUB2FD/TUB4RD were used to amplify portions of the ITS, LSU, RPB2, and TUB genes, respectively (Chen et al, 2015). The obtained sequences (Accession numbers: PP033662 to PP033664 for ITS, PP033667 to PP033669 for LSU, PP035531 to PP035533 for RPB2, and PP035534 to PP035536 for TUB) were 97% identical to that of a B. exigua strain CBS 431.74 (accession no. FJ427001, EU754183, GU371780, and FJ427112) (Table 1). The constructed maximum likelihood tree indicated close relationships between three isolates and B. exigua, which clustered together (Fig. 3). Cowpea plants (cultivar Junlintianxia) at the three-leaf stage were inoculated by spraying a spore suspension (1×106 conidia/ml) of JNJD-1, JNJD-2, and JNJD-3 until run off and incubated at greenhouse conditions (25°C and 12 h light). Inoculations with sterile water were used as a control and each treatment was repeated 3 times with five plants per replicate. Small brown spots appeared on the infected leaves at 2 dpi, followed by the appearance of large blotches, with dark brown at the margin and grayish-white in the center at 5 dpi (Fig. 4A). These lesions gradually increase and coalesce, causing leaf chlorosis and finally defoliation in serious cases. Disease incidence in inoculated cowpea plants treated with the isolates JNJD-1, JNJD-2, and JNJD-3 reached almost 100%. In contrast, control plants developed no symptoms (Fig. 4B). The pathogens were re-isolated from the inoculated leaves and identified as B. exigua using morphological and molecular analysis, whereas no fungus was isolated from control leaves. The experiment was repeated once under the same conditions, yielding similar results. B. exigua has a broad host range, infecting 19 families and 31 genera of plant species, and causing leaf spots, leaf blight, and tuber rot (Lan and Duan 2022). To our knowledge, this is the first report of the pathogen B. exigua causing spot blight on cowpeas. It has been reported that B. exigua infects leguminous crops from multiple genera, such as field pea, soybean, white clover, and Dumasia villosa (Liu et al, 2023). This study further enriches the host range of this pathogen and the pathogen species of cowpea leaf diseases.
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The reaction rate bottleneck during interconversion between insulating S8 (S) and Li2 S fundamentally leads to incomplete conversion and restricted lifespan of Li-S battery, especially under high S loading and lean electrolyte conditions. Herein, we demonstrate a new catalytic chemistry: soluble semiquinone, 2-tertbutyl-semianthraquinone lithium (Li+ TBAQâ - ), as both e- /Li+ donor and acceptor for simultaneous S reduction and Li2 S oxidation. The efficient activation of S and Li2 S by Li+ TBAQâ - in the initial discharging/charging state maximizes the amount of soluble lithium polysulfide, thereby substantially improve the rate of solid-liquid-solid reaction by promoting long-range electron transfer. With in situ Raman spectra and theoretical calculations, we reveal that the activation of S/Li2 S is the rate-limiting step for effective S utilization under high S loading and low E/S ratio. Beyond that, the S activation ratio is firstly proposed as an accurate indicator to quantitatively evaluate the reaction rate. As a result, the Li-S batteries with Li+ TBAQâ - deliver superior cycling performance and over 5 times higher S utilization ratio at high S loading of 7.0â mg cm-2 and a current rate of 1â C compared to those without Li+ TBAQâ - . We hope this study contributes to the fundamental understanding of S redox chemical and inspires the design of efficient catalysis for advanced Li-S batteries.
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Pursuing a more efficient signal amplification strategy is highly demanded for improving the performance of the promising cathodic photoelectrochemical (PEC) sensors. In this work, we present an extremely effective dual signal amplification strategy by the integration of a Z-scheme nanohybrids-based photocathode with the effective signal modulation of an organic photoelectrochemical transistor (OPECT) device. Specifically, photocathodic gate material of CdTe-BiOBr nanohybrids with a Z-scheme electron-transfer route was designed and synthesized for preliminary improvement of the activity of the photogate; afterward, signal modulation of the OPECT system by the photocathodic gate of CdTe-BiOBr was then accomplished for further signal amplification by 2 orders of magnitude. As a result, the output PEC signal of CdTe-BiOBr was enhanced by 17.5-fold as compared to BiOBr, and the channel current (IDS) of the OPECT device was 117-fold magnified than its gate current (IG) response. Exemplified by tetracycline (TC) as a model target and aptamer as the specific recognition element, a versatile cathodic aptasensing platform was constructed based on the proposed OPECT device. The introduced OPECT aptasensor merits advantages, including a good linear range (1.0 × 10-12 to 1.0 × 10-6 M), a low limit of detection (4.2 × 10-13 M), and superior sensitivity than the traditional PEC methods for TC detection, which represents a universal protocol for developing the innovative photocathodic OPECT sensing platform toward accurate analysis.
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Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Compostos de Cádmio , Pontos Quânticos , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Limite de Detecção , Telúrio , Tetraciclina/análise , Antibacterianos/análiseRESUMO
The construction of high sulfur (S) loading cathode is one of the critical parameters to obtain lithium-sulfur (Li-S) batteries with high energy density, but the slow redox reaction rate of high S loading cathode limits the development process. In this paper, a metal coordinated polymer-based three-dimensional network binder, which can improve the reaction rate and stability of S electrode. Compared with traditional linear polymer binders, the metal coordinated polymer binder can not only increase the load amount of S through the three-dimensional cross-linking, but also promote the interconversion reactions between S and lithium sulfide (Li2 S), avoiding the passivation of electrode and improving the stability of the positive electrode. At an S load of 4-5 mg cm-2 and an E/S ratio of 5.5 µL mg-1 , the discharged voltage in the second platform is 2.04 V and the initial capacity is 938 mA h g-1 with metal coordinated polymer binder. Moreover, the capacity retention rate approaches 87% after 100 cycles. In comparison, the discharged voltage in the second platform is lost and the initial capacity is 347 mA h g-1 with PVDF binder. It demonstrates the advanced properties of metal-coordinated polymer binders for improving the performance of Li-S batteries.
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Because it is difficult to isolate standard antimicrobial peptides (AMPs) using traditional biochemical approaches, we designed, synthesized, and evaluated a series of structurally altered histone-derived AMPs (HDAPs) from the pearl oyster Pinctada fucata martensii using molecular cloning approaches. Four histone-homolog genes (PmH2A, PmH2B, PmH3, and PmH4-1) were identified, of which PmH2A and PmH2B had yet to be described. PmH2A and PmH2B were therefore cloned using Rapid Amplification of cDNA Ends (RACE) and characterized. Constitutive PmH2A and PmH2B mRNA expression was detected in all six pearl oyster tissues tested, with comparatively greater transcript abundance in the gonads. Because α-helical content, hydrophilicity index, and the presence of a proline hinge may be the three important factors influencing the antimicrobial efficacy of HDAPs, we synthesized a series of eight N- and C-terminally truncated or amino acid-substituted synthetic candidate HDAP analogs derived from PmH2A, PmH2B, PmH3, and PmH4-1. Only the PmH2A- and PmH4-derived AMPs inhibited bacterial growth. The PmH2A-derived AMPs were α-helical proteins, while the PmH4-derived AMPs were extended strand/random coil proteins. Our results suggested that having an α-helical structure was particularly important for the antibacterial efficacy of the PmH2A-derived peptides; amphipathic structures (hydrophilic index, 0.3 to -0.3) may enhance the antimicrobial function of both the PmH2A- and PmH4-derived peptides. The high antibacterial efficacy of one of the HDAP analogs studied, PmH2A-AMP (5-13) [KLLK]3, indicated that this protein may represent a promising candidate for the treatment of bacterial infections in aquaculture mollusk species. This first study of HDAPs from the pearl oyster P. f. martensii provides new insights into the design and function of highly effective antimicrobial peptides.
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Pinctada , Animais , Pinctada/metabolismo , Histonas/metabolismo , Peptídeos Antimicrobianos , Peptídeos/farmacologiaRESUMO
Antimicrobial peptides (AMPs) play important roles in the immune defense against pathogenic microorganisms. For instance, histone 2A (H2A)-derived AMPs is an antimicrobial peptide involved in the host's innate immune defense and immunoregulation. AMPs have been isolated from the pearl oyster Pinctada fucata martensii but their role in host defense remains poorly understood. To elucidate the structural features of P. f. martensii H2A (PmH2A)-derived AMPs and their potential immune functions, we synthesized a series of laboratory-designed synthetic analogs of PmH2A and examined their antimicrobial properties, as well as their mechanisms of action. This analysis revealed inhibitory effects on the growth of Gram-positive and Gram-negative bacteria. Further assessment by transmission electron microscopy (TEM) of two of the three peptides, PmH2A-AMP and PmH2A-AMP(5-13)[KLLK]3, confirmed that it exerted an anti-bacterial activity through membrane lysis. Finally, we found that the hemocytes and gills of P. f. martensii released antimicrobial H2A histones in response to LPS exposure, mimicking tissue damage and infection. This immune response is reminiscent of the neutrophil extracellular traps (NETs) recently described in oysters. Thus, the LPS challenge is sufficient to induce histone-derived peptide accumulation in pearl oyster P.f. martensii.
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Pinctada , Animais , Histonas , Peptídeos Antimicrobianos , Lipopolissacarídeos/farmacologia , Antibacterianos/farmacologia , Bactérias Gram-Negativas , Bactérias Gram-Positivas , Peptídeos/farmacologiaRESUMO
BACKGROUND: Primary osteoporosis refers to a disease of aging characterized by reduced bone mass, damage to bone tissue microarchitecture, and predisposition to fracture.Sling core stabilization training emphasizes activating the deep local muscles of the spine under unstable conditions, and enhancing the body's balance and control during exercise. CASE PRESENTATION: A 70-year-old female went to the Acupuncture and Rehabilitation Department due to low back pain caused by osteoporosis.The patient received sling core stabilization training three times a week based on Calcium and Vitamin D Supplementation. After training, the patient's back pain was significantly relieved and insisted one year. In the physical examination of bone mineral density, the results showed that the value of bone mineral density was better than last year.The patients adhered to sling core stabilization training and observed the changes of bone mineral density every year basis on calcium and vitamin D supplementation. DISCUSSION: However, cases of calcium and vitamin D supplementation-based regular sling core stabilization training that improves bone density in osteoporosis patients have been rarely reported. Our group shared cases and analyzed possible mechanisms, hoping to provide reference for the prevention and treatment of primary osteoporosis.
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Densidade Óssea , Osteoporose , Feminino , Humanos , Idoso , Cálcio , Osteoporose/complicações , Osteoporose/terapia , Cálcio da Dieta , Vitamina D/uso terapêutico , Suplementos NutricionaisRESUMO
Polymer based quasi-solid-state electrolyte (QSE) has attracted great attention due to its assurance for high safety of rechargeable batteries including lithium metal batteries (LMB). However, it faces the issue of low ionic conductivity of electrolyte and solid-electrolyte-interface (SEI) layer between QSE and lithium anode. Herein, we firstly demonstrate that the ordered and fast transport of lithium ion (Li+ ) can be realized in QSE. Due to the higher coordination strength of Li+ on tertiary amine (-NR3 ) group of polymer network than that on carbonyl (-C=O) group of ester solvent, Li+ can diffuse orderly and quickly on -NR3 of polymer, significantly increasing the ionic conductivity of QSE to 3.69â mS cm-1 . Moreover, -NR3 of polymer can induce in situ and uniform generation of Li3 N and LiNx Oy in SEI. As a result, the Li||NCM811 batteries (50â µm Li foil) with this QSE show an excellent stability of 220â cycles at ≈1.5â mA cm-2 , 5 times to those with conventional QSE. LMBs with LiFePO4 can stably run for ≈8300â h. This work demonstrates an attractive concept for improving ionic conductivity of QSE, and also provides an important step for developing advanced LMB with high cycle stability and safety.
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Polymer-based quasi-solid-state electrolyte (QSE) is an effective means to solve the safety problem of lithium (Li) metal batteries, and stable solid-electrolyte-interface (SEI) layers between electrolyte and anode/cathode are highly required for their long-term stability. Herein, it is demonstrated that a silicon-doped polyether functions as a multifunctional unit, which can induce the formation of stable and robust SEI layers with rich Lix SiOy on both the surfaces of cathode and anode. It simultaneously solves the compatibility of electrolyte and electrodes in the quasi-solid-state Li-metal battery. Moreover, the robust polymer skeleton with a cross-linked network is beneficial to inhibit liquid volatilization and improve battery safety. The assembled Li|QSE|LiFePO4 batteries show a capacity retention rate as high as 97.5% after 400 cycles at 1 C (30 °C), and reach 78.1% after 1000 cycles. Furthermore, there is almost no attenuation of reversible capacity after 100 cycles for the assembled Li|QSE|LiNi0.8 Mn0.1 Co0.1 O2 batteries. The concept of silicon-doped polymer with a crosslinking structure provides an important strategy for designing solid-state or quasi-solid-state polymer electrolytes for the stable long-term operation of both anode and cathode.
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Trypsin-like serine proteases (TLSs) play various roles in dietary protein digestion, hemolymph coagulation, antimicrobial peptide synthesis, and, in particular, the rapid immune pathways activated in response to pathogen detection. The cultured pearl industry, of which Pinctada fucata martensii is one of the most important species, is plagued by disease, thus leading to large economic losses. Herein, the molecular mechanisms underlying the innate immune response of P.f. martensii were explored. First, immune effector molecules from the P.f. martensii genome were screened and a TLS-like gene encoding a protein with a trypsin domain, herein designated as PmTLS, was identified. A multi-sequence alignment indicated a low sequence homology between PmTLS and other mollusk TLS-like proteins. Furthermore, a neighbor-joining phylogenetic analysis indicated that PmTLS has the closest genetic relationship to a Crassostrea gigas TLS. Additionally, real-time quantitative PCR (qPCR) analysis showed that PmTLS mRNA is constitutively expressed in all of the 6 examined P.f. martensii tissues, with significantly higher expression noted in hemocytes relative to the other tissues examined (p < 0.05). P.f. martensii samples were then challenged with various pathogen-associated molecular patterns (PAMPs), including lipopolysaccharide, peptidoglycan, and polyinosinic acid. In the challenge groups, PmTLS was significantly upregulated in hemocytes at 48 h post-challenge when compared to the unchallenged controls. Furthermore, treatment with recombinant PmTLS (rPmTLS) also significantly inhibited the growth of most of the examined gram-negative bacteria tested in vitro (p < 0.05), but it had little effect on the growth of the examined gram-positive bacteria. When examining morphological changes via transmission electron microscopy, rPmTLS treated bacteria exhibited morphological changes such as plasma wall separation. Thus, rPmTLS appears to play a bactericidal role by destroying bacterial cell membranes or cell walls, which subsequently leads to a release of the cellular contents and cell death. The findings presented herein have enabled further characterization of the immune defense mechanisms in P.f. martensii and may lead to improved disease control methods for the pearl cultivation industry.
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Pinctada , Sequência de Aminoácidos , Animais , Clonagem Molecular , Filogenia , Pinctada/genética , Serina Endopeptidases , Tripsina/genéticaRESUMO
Implanting a spherical nucleus into a recipient oyster is a critical step in artificial pearl production using the pearl oyster Pinctada fucata martensii. However, little is known about the role of post-translational modifications (PTMs) in the response of the pearl oyster to this operation. Lysine acetylation, a highly conserved PTM, may be an essential adaptive strategy to manage multiple biotic or abiotic stresses. We conducted the first lysine acetylome analysis of the P. f. martensii gill 12 h after nucleus implantation, using tandem mass tags (TMT) labeling and Kac affinity enrichment. We identified 2443 acetylated sites in 1301 proteins, and 1511 sites on 895 proteins were quantitatively informative. We found 25 conserved motifs from all of the identified lysine sites, particularly motifs Kac H, Kac S, and Kac Y were strikingly conserved, of which Kac Y, Kac H, Y Kac, Kac K, Kac *K, Kac R, and Kac F which have been observed in other species and are therefore highly conserved. We identified 58 sites that were significantly differently acetylated in P. f. martensii in response to allograft (|fold change|>1.2, P-value ≤ 0.05); 38 newly acetylated and 20 deacetylated. According to GO functional analysis, subcellar location, and KOG classIfication, these proteins were divided into four categories: cytoskeleton, response to stimulus, metabolism, and other. The differentially acetylated proteins (DAPs) enriched pathways include aminoacyl-tRNA biosynthesis, salmonella infection, and longevity regulating pathway-worm-Caenorhabditis elegans (nematode). Parallel reaction-monitoring (PRM) validation of the differential acetylation of 10 randomly selected differentially acetylated sites from the acetylome analysis. These results indicated that our acetylome analysis results were sufficiently reliable and reproducible. These results provide an essential resource for in-depth exploration of the stress responses and adaptation mechanisms associated with lysine acetylation in marine invertebrates and P. f. martensii.
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Pinctada , Acetilação , Aloenxertos , Animais , Lisina , Pinctada/genética , Pinctada/metabolismo , Processamento de Proteína Pós-Traducional , RNA de Transferência/metabolismoRESUMO
Redox molecules (RMs) as electron carriers have been widely used in electrochemical energy-storage devices (ESDs), such as lithium redox flow batteries and lithium-O2 batteries. Unfortunately, migration of RMs to the lithium (Li) anode leads to side reactions, resulting in reduced coulombic efficiency and early cell death. Our proof-of-concept study utilizes a biphasic organic electrolyte to resolve this issue, in which nonafluoro-1,1,2,2-tetrahydrohexyl-trimethoxysilane (NFTOS) and ether (or sulfone) with lithium bis(trifluoromethane)sulfonimide (LiTFSI) can be separated to form the immiscible anolyte and catholyte. RMs are extracted to the catholyte due to the enormous solubility coefficients in the biphasic electrolytes with high and low polarity, resulting in inhibition of the shuttle effect. When coupled with a lithium anode, the Li-Li symmetric, Li redox flow and Li-O2 batteries can achieve considerably prolonged cycle life with biphasic electrolytes. This concept provides a promising strategy to suppress the shuttle effect of RMs in ESDs.
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OBJECTIVE: To enhance the biodesulfurization rate using a kinetic model that directs the ratio of Dsz enzymes. RESULTS: This study established a kinetic model that predicted the optimal ratio of Dsz enzymes in the 4S biodesulfurization system to be A:B:C = 1:2:4 and 1:4:2. When BCAD+1A+4B+2C, the conversion rate of dibenzothiophene (DBT) to 2-hydroxybiphenyl (HBP) was close to 100% in vitro. When the gene dose of dszC was increased, the HBP yield of the recombinant strain BL21(DE3)/BCAD + C reached approximately 0.012 mM in vivo, which was approximately 6-fold higher than that of the BCAD strain. CONCLUSIONS: According to the results predicted by the enzyme kinetic model, maintaining higher concentrations of DszC and DszB in the desulfurization system can effectively improve the desulfurization efficiency.
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Proteínas de Bactérias/metabolismo , Enzimas/metabolismo , Modelos Biológicos , Enxofre/metabolismo , Proteínas de Bactérias/genética , Clonagem Molecular , Enzimas/genética , Escherichia coli/genética , Cinética , Redes e Vias MetabólicasRESUMO
The high activity and broad weed spectrum of BS has made it widely used in China. However, accidental crop injuries, particularly occurring in Jiangsu, Hunan, Hubei and Heilongjiang provinces in recent years, have resulted in limiting the application of BS in China. In this study, glutathione homeostasis was measured in the contrasting sensitivity of indica and japonica rice cultivar after bispyribac-sodium (BS) treatment. The results showed that japonica rice cultivar Nanjing 9108 was more sensitive to BS than indica rice Nanjing 11 and indica-hybrid cultivar Guangliangyou 6326. In response to the exposure of BS in all rice cultivars, especially Nanjing 9108, the perturbation of glutathione homeostasis occurred, including the decreased reduced glutathione (GSH) and increased oxidized glutathione (GSSG). These results were supported by increased activities of glutathione S-transferases (GSTs) in Nanjing 11 and Guangliangyou 6326. Further tests revealed that when Nanjing 11 was pretreated with the glutathione-depleting agents L-buthionine-sulfoximine (BSO) or diethylmaleate (DEM), the GSH levels, the activity of GSTs, and the gene expression levels of GR and GSTs decreased, finally increasing the phytotoxicity of BS. The aforementioned DEM inhibitory responses were further rescued by exogenously applied GSH. In contrast, the pretreatment of glutathione or N-acetyl-L-cysteine (NAC) not only increased the contents of GSH, the activities of GSTs, and the expression level of GR and GSTs gene, but also alleviated BS phytotoxicity in Nanjing 9108. In both cultivars, DEM increased phytotoxicity and GSH partially reversed this. This study suggests that increasing tolerance to BS was able to allow glutathione homeostasis to recover in indica rice cultivar compared with japonica rice cultivar.
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Benzoatos/toxicidade , Glutationa/metabolismo , Herbicidas/toxicidade , Oryza/efeitos dos fármacos , Proteínas de Plantas/metabolismo , Pirimidinas/toxicidade , Resistência a Herbicidas , Homeostase , Oryza/metabolismoRESUMO
OBJECTIVE: To develop an algorithm for the automated localization and measurement of levator hiatus (LH) dimensions (AI-LH) using 3-D pelvic floor ultrasound. METHODS: The AI-LH included a 3-D plane regression model and a 2-D segmentation model, which first achieved automated localization of the minimal LH dimension plane (C-plane) and measurement of the hiatal area (HA) on maximum Valsalva on the rendered LH images, but not on the C-plane. The dataset included 600 volumetric data. We compared AI-LH with sonographer difference (ASD) as well as the inter-sonographer differences (IESD) in the testing dataset (n = 240). The assessment encompassed the mean absolute error (MAE) for the angle and center point distance of the C-plane, along with the Dice coefficient, MAE, and intra-class correlation coefficient (ICC) for HA, and included the time consumption. RESULTS: The MAE of the C-plane of ASD was 4.81 ± 2.47° with 1.92 ± 1.54 mm. AI-LH achieved a mean Dice coefficient of 0.93 for LH segmentation. The MAE on HA of ASD (1.44 ± 1.12 mm²) was lower than that of IESD (1.63 ± 1.58 mm²). The ICC on HA of ASD (0.964) was higher than that of IESD (0.949). The average time costs of AI-LH and manual measurement were 2.00 ± 0.22 s and 59.60 ± 2.63 s (t = 18.87, p < 0.01), respectively. CONCLUSION: AI-LH is accurate, reliable, and robust in the localization and measurement of LH dimensions, which can shorten the time cost, simplify the operation process, and have good value in clinical applications.
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Imageamento Tridimensional , Diafragma da Pelve , Ultrassonografia , Humanos , Diafragma da Pelve/diagnóstico por imagem , Imageamento Tridimensional/métodos , Ultrassonografia/métodos , Feminino , Adulto , Pessoa de Meia-Idade , Algoritmos , Idoso , Adulto Jovem , Reprodutibilidade dos TestesRESUMO
At present, the V-Ti-Co phase diagram is not established, which seriously hinders the subsequent development of this potential hydrogen permeation alloy system. To this end, this article constructed the first phase diagram of the V-Ti-Co system by using the CALculation of PHAse Diagrams (CALPHAD) approach as well as relevant validation experiments. On this basis, hydrogen-permeable VxTi50Co50-x (x = 17.5, 20.5, , 32.5) alloys were designed, and their microstructure characteristics and hydrogen transport behaviour were further studied by XRD, SEM, EDS, and so on. It was found that six ternary invariant reactions are located in the liquidus projection, and the phase diagram is divided into eight phase regions by their connecting lines. Among them, some alloys in the TiCo phase region were proven to be promising candidate materials for hydrogen permeation. Typically, VxTi50Co50-x (x = 17.5-23.5) alloys, which consist of the primary TiCo and the eutectic {bcc-(V, Ti) and TiCo} structure, show a high hydrogen permeability without hydrogen embrittlement. In particular, V23.5Ti50Co26.5 exhibit the highest permeability of 4.05 × 10-8 mol H2 m-1s-1Pa-0.5, which is the highest value known heretofore in the V-Ti-Co system. The high permeability of these alloys is due in large part to the simultaneous increment of hydrogen solubility and diffusivity, and is closely related to the composition of hydrogen permeable alloys, especially the Ti content in the (V, Ti) phase. The permeability of this alloy system is much higher than those of Nb-TiCo and/or Nb-TiNi alloys.
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The precise liquidus projection of the V-Ti-Fe system are crucial for designing high-performance hydrogen permeation alloys, but there are still many controversies in the research of this system. To this end, this article first uses the CALPHAD (CALculation of PHAse Diagrams) method to reconstruct the alloy phase diagram and compares and analyses existing experimental data, confirming that the newly constructed phase diagram in this article has good reliability and accuracy. Second, this obtained phase diagram was applied to the subsequent development process of hydrogen permeation alloys, and the (Ti65Fe35)100-xVx (x = 0, 2.5, 5, 10, 15, 25) alloys with dual-phase {bcc-(V, Ti) + TiFe} structure were successfully explored. In particular, the alloys with x values equal to 2.5 at.% and 5 at.% exhibit relatively high hydrogen permeability. Third, to further increase the H2 flux permeation through the alloys, a 500-mm-long tubular (Ti65Fe35)95V5 membrane for hydrogen permeation was prepared for the first time. Hydrogen permeation testing showed that this membrane had a very high H2 flux (4.06 mL min-1), which is ca. 6.7 times greater than the plate-like counterpart (0.61 mL min-1) under the same test conditions. This work not only indicates the reliability of the obtained V-Ti-Fe phase diagram in developing new hydrogen permeation alloys, but also demonstrates that preparing tubular membranes is one of the most important means of improving H2 flux.