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Enhanced recovery after surgery (ERAS) protocols were first reported for colorectal and gynaecologic procedures. The main benefits have been a shorter length of stay and reduced complications. A retrospective audit was conducted of all patients who underwent ambulatory pelvic organ prolapse surgery at the Townsville Day Surgery between January 2008 and June 2019. Following the publication of a former audit, a modified ERAS protocol was adopted at our practice. We omitted a carbohydrate-rich fluid intake prior to surgery in our local protocol. Data were analysed for the type of surgery, postoperative complications, and readmissions. All surgeries were performed by a single consultant urogynecologist. A total of 1381 women underwent 1937 surgeries. Transvaginal mesh (55.8%) was the commonest procedure, followed by a posterior repair (23.9%). Ninety-five patients (4.4%) had various complications, with a failed trial of the void as the commonest complication (87 patients). Only 8 patients (0.4%) required an unplanned admission after their procedures. Site-specific ERAS protocol is effective for providing standardised care in the surgical treatment of women with pelvic organ prolapse and urinary incontinence in an outpatient setting. Complication rates are low and reduce further with experience and familiarity with the protocol.Impact statement:What is already known on this subject? Enhanced recovery after surgery (ERAS) protocols are being increasingly used in surgical practices. The main objective of the protocol has been on reducing postoperative morbidity and stay. The commonly practiced protocol includes a high protein intake diet and a glucose drink prior to surgery.What do the results of this study add? Our study utilises a modified ERAS protocol of omitting the high protein diet and the glucose drink for the minimally invasive uro-gynaecology procedures. The modified protocol is safe and associated with lower complications and readmission rates.What are the implications of these findings for clinical practice and/or further research? The present study demonstrates the safety and effectiveness of our modified ERAS protocol that allows for patients to undergo surgical procedures and discharge on the same day, thus minimising the impact on the quality of life and vocations. A multi-center randomised controlled trial will conclusively demonstrate a cause-effect relationship between early discharge and patient preparation with our modification of the ERAS protocol. Further research should also consider patient satisfaction as an additional outcome measure.
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Recuperação Pós-Cirúrgica Melhorada , Prolapso de Órgão Pélvico , Feminino , Glucose , Humanos , Tempo de Internação , Estudos Multicêntricos como Assunto , Prolapso de Órgão Pélvico/cirurgia , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/prevenção & controle , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos RetrospectivosRESUMO
The present study was undertaken at the Port Moresby General Hospital, Papua New Guinea, to evaluate the ease of use and acceptability of a perineal measuring device (Episiometer) for giving a correct length and angle of a mediolateral episiotomy. An Episiometer was placed on the perineum to guide the angle and length immediately before giving an episiotomy. A feedback survey was collected and the angle and length of the episiotomy were measured at the time of delivery and at 6 weeks postpartum. The length and angle of episiotomy were found to be accurate in 86% of the cases. Forty-two of the fifty (86%) clinicians felt that the Episiometer was easy or very easy to use. They also found this device to be beneficial (92%). The Episiometer seems to be an easy-to-use and feasible device that is well accepted by clinicians and patients and may aid in standardising the length and angle of an episiotomy. Impact statement What is already known on this subject? Episiotomy is the commonest obstetric procedure performed worldwide. However, the angle and length of an episiotomy vary greatly amongst operators. It is also recognised that birth trauma and an inaccurate episiotomy result in debilitating anal sphincter injury. Therefore, a pilot study was conducted to evaluate the ease of use and acceptability of a new, inexpensive, low- tech device "Episiometer" to guide clinicians and midwives to perform an appropriate episiotomy. What do the results of this study add? This pilot study to evaluate the usability of the new innovation amongst clinician and patients revealed high acceptance of the device amongst clinicians and positive attitude of the patients towards the device. The clinicians also felt the device to be an effective teaching tool. Usage if the device results in a more accurate length and angle of an episiotomy. What are the implications of these findings for clinical practice and/or further research? Further research and multicentre randomised control trials are needed to establish the effectiveness of the device in reducing the complications of episiotomy and risk of OASIS. The present study has shown the Episiometer as an acceptable and easy to use device amongst clinicians and can benefit the young clinicians as a teaching tool in directing a correct angle and length of episiotomy.
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Episiotomia/instrumentação , Adolescente , Adulto , Episiotomia/efeitos adversos , Episiotomia/métodos , Feminino , Humanos , Papua Nova Guiné , Satisfação do Paciente , Projetos Piloto , Gravidez , Adulto JovemRESUMO
BACKGROUND & OBJECTIVES: Presence of psychological morbidity in medical undergraduate students has been reported from various countries across the world. Indian studies to document this burden are very few. Therefore, the presence of depression, anxiety and stress among medical undergraduate students was assessed using a previously validated and standardized instrument, Depression Anxiety Stress Scale (DASS 42) and the associations with their socio-demographic and personal characteristics were identified. METHODS: In a cross-sectional survey, a self-administered, pre-designed, pre-tested anonymous questionnaire including DASS 42 was used to collect information on basic socio-demographic (age, gender, semester) and personal characteristics (alcohol and tobacco use, academic performance). All students present on the day of survey were contacted for participation after obtaining informed written consent. Scores for each of the respondents over each of the sub-scales (Depression, Anxiety and Stress) were calculated as per the severity-rating index. RESULTS: More than half of the respondents were affected by depression (51.3%), anxiety (66.9%) and stress (53%). Morbidity was found to be more in 5 th semester students rather than students of 2 nd semester. Females reported higher score as compared to their male counterparts. Perception of self assessment in academics was strongly associated with the higher score. CONCLUSIONS: A substantial proportion of medical undergraduate students was found to be depressed, anxious and stressed revealing a neglected area of the students' psychology requiring urgent attention. Student counselling services need to be made available and accessible to curb this morbidity.
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Transtornos de Ansiedade/psicologia , Transtorno Depressivo/psicologia , Estresse Psicológico/psicologia , Estudantes de Medicina/psicologia , Adulto , Transtornos de Ansiedade/epidemiologia , Transtorno Depressivo/epidemiologia , Feminino , Humanos , Índia , Masculino , Estresse Psicológico/epidemiologia , Inquéritos e Questionários , UniversidadesRESUMO
BACKGROUND: The decision to make a quit attempt is the first step toward the tobacco cessation process. It is well established in the literature that if someone does not take tobacco till the age of 21 years then his chances of remaining tobacco-free for life are higher than his counterparts who start tobacco at early developmental ages. METHODOLOGY AND TOOLS: The present study was conducted among 400 university undergraduate students. A cross-sectional survey design was used, multi-stage sampling was done, and four colleges were selected via random sampling. The motivation to quit tobacco, tobacco craving, and maintenance of tobacco abstinence was assessed via contemplation ladder, tobacco craving questionnaire Short Form, and smoking abstinence questionnaire. To validate subjective data, a urine cotinine test was performed. RESULTS: The age of tobacco initiation significantly impacts intentions to quit tobacco and tobacco craving levels. The number of unsuccessful quit attempts was also significantly associated with the maintenance of tobacco abstinence. The failed quit attempts play a vital role in altering tobacco cravings and make the withdrawals more complicated to handle for recent tobacco quitters.
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Abandono do Hábito de Fumar , Humanos , Adulto Jovem , Adulto , Abandono do Hábito de Fumar/métodos , Estudos Transversais , Fumar , Nicotiana , MotivaçãoRESUMO
ABSTRACT: The provision of breast cancer care tends to vary substantially from one breast unit to another. To provide state-of-the-art patient-centered care to women diagnosed with breast cancer, adoption and adherence to structured treatment algorithms, protocols, and international guidelines are essential. In this review, we endeavor to audit breast cancer care at our tertiary cancer center against published EUSOMA guidelines. This was a retrospective study with an observational design. All patients who completed recommended treatment for breast diseases at our institute from January 1, 2018 to December 31, 2018 were included and evaluated. Data were retrieved from patient e-prescriptions and medical records. Analysis was performed using Microsoft Office 2010 to measure how our practices compared to EUSOMA quality indicators (QIs). Clinical assessments, imaging, and preoperative work-up of breast cancer patients met EUSOMA standards. Prognostic and predictive characterization of tumors was performed in all cases. Surgical management of invasive cancer and ductal carcinoma in situ (DCIS) was in accordance with the guidelines. Adherence to postoperative radiation and adjuvant endocrine therapy was adequate. More mastectomies were performed in patients with invasive cancers measuring <3 cm. Overtreatment was avoided in every other subgroup. Adjuvant and neoadjuvant chemotherapy and targeted adjuvant therapy were adequately utilized unlike neoadjuvant targeted therapy. Minimal attrition was noted in patient follow-up. This extensive audit has set a benchmark for future annual audits and helped highlight areas where improvement of service delivery is needed.
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Introduction: The year 2020 brings in a paradigm shift in medical education with the emergence of the COVID-19 pandemic. Since the government of India initiated the first countrywide lockdown, educational institutions, including medical colleges, have adopted online mode for undergraduate teaching. Medical education is virtually incomplete without appropriate practical exposure. This study was conducted to understand the opinion of the medical students towards online classes as an impact of this pandemic on medical education. Materials and Methods: A descriptive online survey was conducted using a google form among medical undergraduate students from across India. Snowball sampling was used to recruit study participants. The responses were analyzed using numbers and percentages. Continuous variables were expressed as the mean and standard deviation of the mean. Results: The mean age of the 1426 respondents was 20.46 years. The majority of the responses were from Karnataka (412), followed by Odisha (167). While fairly a good number of respondents agreed that online classes provided a conducive environment and independence in learning, a large proportion disagreed or remained neutral in terms of its usefulness. This could be attributed to several reasons, which may be categorized into three important groups: the technical competence of the students, the provision of an enabling environment and the ability of the teachers. Conclusion: Students had varying opinions on online classes, but most emphasized the need for redoing the lessons taught online.
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Pressão Sanguínea , Pesos e Medidas Corporais , Pré-Hipertensão/epidemiologia , Estudantes de Medicina/estatística & dados numéricos , Adolescente , Índice de Massa Corporal , Estudos Transversais , Educação de Graduação em Medicina , Feminino , Humanos , Índia/epidemiologia , Masculino , Pré-Hipertensão/fisiopatologia , Prevalência , Circunferência da Cintura , Relação Cintura-Quadril , Adulto JovemRESUMO
Introduction: Antitobacco media messages can easily reach the mass and play a very positive and significant role in changing the motivational stages among recent quitters. Motivation is the key to changing human behaviour. Motivation can be intrinsic and extrinsic. To modify tobacco-related behaviour, one must have an inherent motivation to quit tobacco. However, the outside factors, for example, protobacco advertisements, antitobacco advertisements, peer pressure, celebrity influence, and family members' influence cannot be ignored. Method: A total of 400 recent tobacco quitters were enrolled from four colleges via a multistage sampling method. Time series research design was used for data collection at three time periods 0, 1, and 3 months. Study participants were divided into four groups: 1) personal testimony group, 2) health warning group, 3) celebrity-influenced public service announcements, and 4) natural exposure group. Media messages containing antitobacco video clippings and pictures were delivered to the participants via phone thrice a week, as per the groups assigned. All four groups were assessed for the motivational stage via contemplation ladder at 0, 1, and 3 months of intervals. Results: Antitobacco personal testimonial media messages are most effective in enhancing the motivation to quit tobacco, followed by the antitobacco health warning messages, which are also proven to be effective in maintaining high motivation levels to remain abstinent from smoking. However, public service announcements are ineffective in keeping the motivation to quit tobacco at higher smoking. Conclusion: Overall, the antitobacco state-sponsored media messages, personal testimonials, and health warnings about tobacco products effectively maintain and enhance motivation to quit tobacco.
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Background: Globally, anemia is one of the biggest public health challenges. The highest prevalence of anemia is seen among adolescents. Tribal communities are especially at a disadvantage, with several studies documenting a high prevalence of anemia among tribal adolescents. We investigated the prevalence of anemia and its associated risk factors among the tribal residential adolescent school students in Odisha. Material and Methods: In a cross-sectional survey, the prevalence of anemia was estimated by spectrophotometry among adolescents of residential schools in three predominantly tribal districts of Odisha. The severity of anemia was defined as per the World Health Organization classification for adolescents. Results: The mean age of 953 subjects was 13.07 ± 1.48 years. The prevalence of anemia was found to be 37.3%. As per the World Health Organization classification, 19.9% had mild anemia, 16.3% had moderate, and 1% had severe anemia. Consumption of Iron Folic Acid (IFA) was associated with the level of hemoglobin at a statistically significant level. Conclusion: We found that the prevalence of anemia was lower than in similar studies conducted in other parts of the country. Despite poor coverage of beneficiaries with iron and folic acid at the national level, our study showed better compliance and was associated with a significantly higher level of hemoglobin among those who consumed IFA.
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BACKGROUND: Sleep disorders are common in childhood and adolescence with mental and physical consequences needing appropriate intervention by caregivers and health care providers (HCPs). The objective of the study, conducted at a tertiary teaching hospital and public school in north India, was to develop validated questionnaires to evaluate childhood and adolescent sleep awareness in caregivers and HCPs. METHODS: The study participants (caregivers represented by parents of 2-18 year olds attending a public school and of those attending outpatient services at the study hospital and HCPs represented by medical interns and nursing graduates within 1 year of graduation) were enrolled after appropriate screening. RESULTS: Two separate questionnaires in English for caregivers (also translated in Hindi) and HCPs were applied on 313 caregivers and 175 HCPs (110 medical interns and 65 nursing graduates) and developed and validated with a Cronbach α of 0.73 and 0.74, respectively. The questionnaires covered three domains: sleep hygiene, sleep related health problems, and miscellaneous. Both group of respondents had >50% correct responses in sleep hygiene. The ĸ agreement between knowledge and practice of sleep hygiene in caregivers was 0.2. Poor response (<50% correct responses) was seen in sleep-related health problems in both groups of respondents implying knowledge deficit in various sleep disorders. The HCPs performed poorly on basic theoretical questions in miscellaneous domain. For consultation of sleep problems, most caregivers (48%) chose pediatricians. CONCLUSION: There is a need to strengthen undergraduate medical and nursing curriculum in sleep. Caregivers should be made aware of implications of unhealthy sleep.
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Cuidadores , Transtornos do Sono-Vigília , Humanos , Adolescente , Pessoal de Saúde , Inquéritos e Questionários , Sono , HospitaisRESUMO
Paraquat is a highly toxic and persistent pesticide in soil but is still used for wheat crops in many countries. Paraquat can pose potential health hazards if it is translocated from soil into wheat grains, but no study is available for its possible translocation causing wheat grain contamination. The present study aimed at finding out Paraquat residue in wheat grains under field conditions for two crop seasons to explore the sustainability of this pesticide. The experiments were conducted scientifically under field conditions at agricultural fields Pusa, Delhi, India. The soil texture was classified as sandy loam. Paraquat dichloride 24% SL (herbicide) was applied on five fields except for control field. Paraquat in wheat grains was analyzed using HPLC equipped with a photodiode array (PDA) detector. The method of analysis was validated for the pesticide residue recovery. The results showed that there was an alarming concentration of Paraquat in wheat grains ranging between 21.6 and 49.02 mg kg-1 against maximum residue level of 0.1 mg kg-1. Paraquat was also found in control crop (3.1 mg kg-1) due to background residue in soil even when no Paraquat was applied. Furthermore, wheat flour samples from market also gave alarming Paraquat residue (20.39, 25.88, and 27.68 mg kg-1). Paraquat residue was primarily dependent on % clay in field soils. More the % clay lesser was Paraquat residue in wheat grain. Thus, Paraquat was translocated from soil into wheat grains and resulted in worrying concentration of Paraquat residue in wheat grains. Consequently, use of Paraquat for wheat crops needs to be regulated as it contaminated the soil and resulted in the wheat grain contamination posing severe health hazards for humans.
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Herbicidas , Resíduos de Praguicidas , Poluentes do Solo , Argila , Produtos Agrícolas , Grão Comestível/química , Farinha/análise , Herbicidas/análise , Humanos , Paraquat , Resíduos de Praguicidas/análise , Solo/química , Poluentes do Solo/análise , Triticum/químicaRESUMO
Sentinel lymph node biopsy is an established practice to avoid axillary clearance, in clinically negative axilla, in breast cancer patients. Sentinel nodes harvested by dual technique, if found negative on intraoperative frozen section, can prevent breast cancer patient from a potentially debilitating complete axillary clearance. Hence, analyzing the institutional accuracy of this technique and comparing it with international standards, becomes important in providing optimal treatment to these patients. A retrospective analysis of all patients who had undergone sentinel lymph node biopsy at our institute from December 2014 to December 2018 was carried out. At our institute, sentinel lymph nodes are identified using dual technique of methylene blue and radiocolloid dye. Intraoperative frozen section of these hot or blue or any enlarged nodes is performed. Patients with positive frozen section undergo complete axillary clearance. All frozen and unfrozen biopsy material is subjected to further paraffin sectioning and immunohistochemistry. False negative rate and factors associated with were analyzed. A total number of 424 patients had undergone intraoperative frozen section for the sentinel node in breast cancer at our institute during the study period. Among these, 307 patients had negative sentinel nodes and 117 had positive sentinel nodes of frozen section. Seventeen patients out of 307 had lymph node metastases in final paraffin report (false negative rate = 12.6%). Two of these were found to have macrometastasis, 13 had micrometastasis and 2 had isolated tumor cells on final immunohistochemistry report. Size of metastases to sentinel lymph node was found to be a statistically significant contributor to higher false negative rate. Sentinel lymph node biopsy using intraoperative frozen section, is a sensitive and specific technique of staging axilla in breast cancer patients. Detection of micrometastasis and isolated tumor cells present a technical challenge and are associated with higher false negative rates.
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Enhanced recovery after surgery (ERAS) is a multimodal convention first reported for colorectal and gynecologic procedures. The main benefits have been a shorter length of stay and reduced complications, leading to improved clinical outcomes and cost savings substantially. With increase in life expectancy, recent years has shown a significant rise in advanced age population, and similarly, a rise in age-related disorders requiring surgical management. Due to pathophysiological and metabolic changes in geriatric age group with increased incidence of medical comorbidities, there is higher risk of enhanced surgical stress response with undesirable postoperative morbidity, complications, prolonged immobility, and extended convalescence. The feasibility and effectiveness of ERAS protocols have been well researched and documented among all age groups, including the geriatric high-risk population.[1] Adhering to ERAS protocols after colorectal surgery showed no significant difference in postoperative complications, hospital stay, or readmission rate among various age groups.[2] A recent report mentions the safety and benefits following ERAS guidelines with reduced length of stay in elderly patients with short-level lumbar fusion surgery.[3] The concept of prehabilitation has evolved as an integral part of ERAS to build up physiological reserve, especially in geriatric high-risk group, and to adapt better to surgical stress.[4] High levels of compliance with ERAS interventions combined with prehabilitation can be achieved when a dedicated multidisciplinary team is involved in care of these high-risk patients.
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Hereditary breast cancer (HBC) accounts for 5-10% of all breast cancer patients. Mutations in BRCA 1 and 2 are the most common culprits of HBC. These patients have a much higher lifetime risk of developing breast cancer than the non-carriers. Thus these high-risk patients qualify to receive risk-reducing measures in form of close surveillance, chemoprophylaxis, or sometimes even risk-reducing surgeries in high penetrance mutation carriers. We report a case of bilateral risk-reducing prophylactic mastectomies (B/L RRM) and bilateral risk-reducing salpingo-oophorectomy (B/L RRSO) performed in a 37-year-old healthy BRCA 1 carrier. Although, this is an age-old practice, its acceptance in India has been low for reasons such as cost of surgery, social stigma, lack of awareness, fear of visiting an oncology clinic, surgery and reconstruction, or loss of a healthy organ; and more acceptance towards other risk reduction methods.
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Newer advances in breast cancer management have led to increased disease free survival and overall survival. It is important to prevent debilitating complications after axillary lymph node dissection (ALND) to be able to successfully translate this survival benefit to quality of life benefit. By reducing disruption of lymphatic channels, sentinel lymph node biopsy (SLNB) decreases incidence of lymphedema (LE). Initiating early physiotherapy regimens, too, improves arm symptoms. In this review, we analyze the incidence of LE at our center and compare it with western literature. Retrospective analysis of all post-surgery breast cancer 18 patients, who followed up routinely with our oncophysiotherapist, was carried out. Incidence of LE in patients undergoing SLNB or ALND was followed up for a mean period of 17.5 months. Only 3.6%, i.e., 6 patients out of 166 developed LE. Amongst 166, 80 had only SLNB; the rest had ALND (either upfront or post-positive SLNB). None of the SLNB only cohort patients developed LE. SLNB in clinically node negative axilla, followed by initiation of arm physiotherapy early in post-operative period, may reduce LE incidence in breast cancer patients.
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INTRODUCTION: Coal miners working underground are likely to be at greater risk of developing Vitamin D deficiency due to reduced sunlight exposure. To date, only three studies have been reported, with no significant risk of Vitamin D deficiency among coal miners. None were conducted in the tropical region, where sunlight is ample. OBJECTIVE: The present study estimated Vitamin D levels among underground coal miners in a tropical region and determined their associated factors. METHODS: Serum calcium, 25-hydroxyvitamin D, and bone turnover markers among underground and surface workers were estimated in a matched case-control study. Binary logistic regression analysis was performed using case/control as the dependent variable to estimate odds ratios and 95% confidence intervals of variables which significantly (P < 0.1) differed among the cases and controls. RESULTS: The mean Vitamin D level among both coal miners (22.12 ng/dl) and surface workers (23 ng/dl) were low. No significant difference was noted for all biochemical parameters after adjusting for other covariates except the parathyroid hormone (PTH), which was marginally higher among the surface workers. CONCLUSIONS: Even in the absence of statistically significant evidence of low Vitamin D level in underground workers compared to surface workers, there was indirect evidence (of differences in PTH) to indicate that Vitamin D level is lower in underground workers probably because of poor sunlight exposure.
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BACKGROUND: Early diagnosis of asymptomatic coronary artery disease (CAD) is presently targeted in preventive cardiology. A positive family history though not modifiable can provide a window of opportunity for intervening on modifiable risk factors. We assessed the prevalence of risk factors among the family members of suspected CAD patients and estimated their 10 years CAD risk. METHODS: In a hospital-based cross-sectional study, socio-demographic, personal data and blood samples for total and HDL cholesterol were collected. The risk of having a heart attack in the next 10 years was calculated using Framingham Risk Score. RESULTS: The mean age of participants (n = 60) was 40.55 ± 1.78 years. 85% were physically inactive. Smoking (13%), alcohol use (12%), history of CAD (2%), HTN (12%) and diabetes (22%) were the observed risk factors. A family history of CAD at <40 years of age was reported by 7% of subjects. Prevalence of overweight was 21% and 11% were obese. Increased waist-hip ratio (43%), diabetes (22%), hypercholesterolemia (28%), reduced HDL Cholesterol (48%) were other prevalent risk factors. Risk of CAD of >1% in the next 10 years was noted among 60% of subjects. CONCLUSIONS: Window of opportunity for secondary prevention exists among the family members of suspected CAD.
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International standards for clinical staffing of delivery care in maternity units are currently lacking, with resulting gaps in provision leading to adverse outcomes and very poor experiences of care for women and families. While evidenceinformed modelling approaches have been proposed based on population characteristics and estimated rates of complications, their application and outcomes in lowresource settings have not been reported. Here, FIGO's Safe Motherhood and Newborn Health Committee proposes indicative standards for labor wards as a starting point for policy and program development. These standards consider the volume of deliveries, the case mix, and the need to match clinical care requirements with an appropriate mix of professional skills among midwifery and obstetric staff. The role of Shift Leader in busy labor wards is emphasized. Application of the standards can help to assure women and their families of a safe but also positive birthing experience. FIGO calls for investment by partners to test these clinicallyinformed recommendations for delivery unit staffing at hospital and district level in low and middleincome country settings.
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Parto Obstétrico/métodos , Episiotomia/normas , Adulto , Episiotomia/efeitos adversos , Episiotomia/estatística & dados numéricos , Feminino , Humanos , Lacerações/prevenção & controle , Períneo/lesões , Gravidez , Procedimentos Desnecessários/normasRESUMO
Using male Sprague-Dawley rats implanted with third intracerebroventricular (ICV) cannulae, we found that cilostamide, a phosphodiesterase 3 (PDE3) inhibitor, (i) reversed the established effects of leptin on food intake and body weight, (ii) blocked, at the hypothalamic level, the leptin-induced tyrosine phosphorylation of signal transducer and activator of transcription 3 (Stat3) and (iii) blocked the DNA binding of p-Stat3. Additionally, ICV administration of leptin increased hypothalamic phosphatidylinositol 3-kinase (PI3K) and PDE3B activities and decreased cyclic AMP (cAMP) concentration. These results indicate that a PI3K-PDE3B-cAMP pathway interacting with the Janus kinase 2 (Jak2)-Stat3 pathway constitutes a critical component of leptin signaling in the hypothalamus.