Motor effects of fentanyl in isoflurane-anaesthetized pigs and the subsequent effect of ketanserin or naloxone.

OBJECTIVE: To examine the effect of ketanserin and naloxone on fentanyl-induced motor activity in isoflurane-anaesthetized pigs. STUDY DESIGN: Randomized, blinded, prospective two-group study. ANIMALS: A group of 12 crossbred pigs weighing 22-31 kg. METHODS: Fentanyl was administered to isoflurane-anaesthetized pigs at 7.5 µg kg-1 hour-1 for 40 minutes intravenously, followed by an intravenous injection of naloxone 0.1 mg kg-1 or ketanserin 1 mg kg-1. Electromyography (EMG) and accelerometry were used to record motor unit activity and tremors, respectively. To test the effect of drug administration on motor activity, data from a 5 minute period at baseline, immediately before and after antagonist injection were compared in a mixed model; p < 0.05. RESULTS: Results are reported with the median difference, 95% confidence intervals and corresponding p-values in brackets. Fentanyl significantly increased EMG activity [30.51 (1.84-81.02) µV, p = 0.004] and induced tremors [0.09 (0.02-0.18) m s-2, p < 0.001] in 10 of 12 pigs. Ketanserin significantly reduced EMG [32.22 (6.29-136.80) µV, p = 0.001] and tremor [0.10 (0.03-0.15) m s-2, p = 0.007] activity. No significant effect was found for naloxone on EMG [26.76 (-13.28-91.17) µV, p = 0.4] or tremors [0.08 (-0.01-0.19) m s-2, p = 0.08]. CONCLUSIONS AND CLINICAL RELEVANCE: Fentanyl can induce motor activity in anaesthetized pigs, with a suggested link to the serotonergic system. This study shows that ketanserin can antagonize this activity, which supports the role of serotonin. This knowledge contributes to the general understanding of the motor effects of fentanyl and especially the problem of tremors in anaesthetized pigs.

Rocuronium infusion: A higher rate is needed in diabetic than nondiabetic dogs.

OBJECTIVE: To determine the infusion rates that maintain the train-of-four (TOF) ratio within 20-70% in dogs and compare the infusion rates between diabetic and nondiabetic dogs. STUDY DESIGN: Prospective clinical study. ANIMALS: In total, 47 dogs scheduled for phacoemulsification were included with a median (80% central range) bodyweight of 10.6 (5.7-35.5) kg and age of 7 (1-11) years. Diabetes mellitus was previously diagnosed in nine dogs. METHODS: After premedication using acepromazine and methadone, anaesthesia was induced by intravenous (IV) propofol and maintained by isoflurane and fentanyl or remifentanil. The TOF ratio was monitored by stimulating the peroneal nerve and the response quantified by accelerometry. Rocuronium 0.5 mg kg-1 was administered IV, and further infused to maintain the TOF ratio between 20% and 70%. The infusion rates of rocuronium were compared by the Mann-Whitney test between diabetic and nondiabetic dogs, and the influence of age, sex, bodyweight, body temperature, end-tidal carbon dioxide, end-tidal isoflurane concentration, mean arterial blood pressure, pulse rate and time from induction and time from rocuronium bolus to stable rocuronium infusion rate were investigated in a stepwise, forward regression model. RESULTS: A stable infusion rate was found in 42 dogs. A higher median (80% central range) infusion rate was found in diabetic [0.43 (0.35-0.50) mg kg-1 hour-1] compared with nondiabetic dogs [0.30 (0.20-0.50) mg kg-1 hour-1] (p = 0.013). None of the other variables investigated were found to significantly influence the infusion rate. CONCLUSIONS AND CLINICAL RELEVANCE: There is a quite large individual variation in the infusion rates of rocuronium needed to maintain a stable neuromuscular block in a varied population of dogs. Of the variables investigated, diabetes mellitus was the only one found to significantly influence the infusion rate of rocuronium.

Dexmedetomidine or fentanyl? Cardiovascular stability and analgesia during propofol-ketamine total intravenous anaesthesia in experimental pigs.

OBJECTIVE: To compare cardiovascular function and response to nociception during total intravenous anaesthesia in pigs with propofol, ketamine and either dexmedetomidine or fentanyl administered as a continuous infusion. STUDY DESIGN: Blinded, randomized, balanced, crossover study ANIMALS: Eight immunocastrated male, mixed breed pigs with a mean ± standard deviation body weight of 26.4 ± 1.9 kg for dexmedetomidine and 27.5 ± 3.8 kg for fentanyl treatment. METHODS: The animals were anaesthetized twice with either propofol-ketamine-dexmedetomidine (DEX) or fentanyl (FENT). DEX was infused at 2, 4 and 8 µg kg-1 hour-1 and FENT at 25, 50 and 100 µg kg-1 hour-1. Each infusion rate was administered for 80 minutes prior to commencing the next. Heart rate (HR), 3-lead electrocardiogram, systolic, mean and diastolic arterial blood pressure (SAP, MAP, DAP) in addition to cardiac output measured by transpulmonary thermodilution was used to monitor cardiovascular function. Mechanical and electrical stimulation (nociceptive withdrawal reflex, NWR) was used to elicit nociceptive responses. Similar anaesthetic depth was determined based on the NWR response. Cardiovascular parameters were compared statistically at this time. Additionally, response to nociceptive stimulation and cardiovascular response over time were compared. RESULTS: DEX-treated pigs had significantly higher HR, SAP, DAP, MAP, systemic vascular resistance, haemoglobin concentration, content of oxygen in arterial and venous blood and oxygen delivery index than FENT-treated pigs at similar anaesthetic depth, whereas stroke volume index was significantly higher in FENT. Motoric response to mechanical nociceptive stimulation was abolished prior to any decrease in NWR response in FENT, whereas the two responses decreased more in unison in DEX. The cardiovascular response to nociception was less pronounced in DEX than in FENT. CONCLUSIONS AND CLINICAL RELEVANCE: Propofol combined with ketamine and either fentanyl or dexmedetomidine provides stable cardiovascular conditions in normovolaemic, healthy pigs. Based on cardiovascular response and depression of NWR, dexmedetomidine apparently provides superior analgesia to fentanyl.

The cardiovascular status of isoflurane-anaesthetized horses with and without dexmedetomidine constant rate infusion evaluated at equivalent depths of anaesthesia.

OBJECTIVE: To compare cardiac index and oxygen extraction at equivalent depths of anaesthesia between isoflurane-anaesthetized horses and horses anesthetized with isoflurane and dexmedetomidine CRI. STUDY DESIGN: Sequential, blinded, randomized, balanced, crossover study. ANIMALS: Eight horses weighing a mean ± standard deviation of 478 ± 58 kg. METHODS: Horses were premedicated with 0.03 mg kg(-1) acepromazine intramuscularly (IM) and 8 µg kg(-1) dexmedetomidine intravenously (IV). Anaesthesia was induced with 2.5 mg kg(-1) ketamine and 0.1 mg kg(-1) midazolam IV and maintained with isoflurane in oxygen and air. Horses were mechanically ventilated. Fractional concentration of end-tidal isoflurane (Fe'Iso) was stabilized at 1.7% with a CRI of 0.9% NaCl (IsoNaCl), or at 1.1% with 1.75 µg kg(-1)  hour(-1) dexmedetomidine (IsoDex). Mean arterial blood pressure was maintained above 60 mmHg by dobutamine infusion. Following nociceptive electrical stimulation, Fe'Iso was stabilized at a 0.1% lower concentration and nociceptive stimulation was repeated. This procedure was continued until the horse moved. Fe'Iso values prior to the concentration at which movement occurred were considered to indicate equivalent depths of anaesthesia between treatments. Cardiac index and oxygen extraction were compared at equivalent depths of anaesthesia using a paired Student's t-test. RESULTS: Cardiac index differed between IsoNaCl at 61 ± 12 mL kg(-1)  minute(-1) and IsoDex at 48 ± 10 mL kg(-1)  minute(-1) (p = 0.047). In addition, oxygen extraction differed between IsoNaCl at 3.4 ± 0.8 mL kg(-1)  minute(-1) and IsoDex at 4.5 ± 0.5 mL kg(-1)  minute(-1) (p = 0.0042). Two horses receiving IsoNaCl were administered dobutamine at equivalent depths of anaesthesia (7.0 and 28.8 µg kg(-1)  hour(-1) , respectively). CONCLUSIONS AND CLINICAL RELEVANCE: Cardiovascular function in horses receiving isoflurane and 1.75 µg kg(-1)  minute(-1) dexmedetomidine is more compromised than in horses receiving a higher concentration of isoflurane and 0.9% NaCl CRI.

Antinociceptive effect of buprenorphine and evaluation of the nociceptive withdrawal reflex in foals.

OBJECTIVE: To elicit and evaluate the NWR (nociceptive withdrawal reflex) in 2 and 11 day old foals, to investigate if buprenorphine causes antinociception and determine if the NWR response changes with increasing age. The effect of buprenorphine on behaviour was also evaluated. STUDY DESIGN: Prospective, experimental cross-over trial. ANIMALS: Nine Norwegian Fjord research foals. METHODS: Buprenorphine, 10 µg kg(-1) was administered intramuscularly (IM) to the same foal at 2 days and at 11 days of age. The NWR and the effect of buprenorphine were evaluated by electromyograms recorded from the left deltoid muscle following electrical stimulation of the left lateral palmar nerve at the level of the pastern. Mentation, locomotor activity and respiratory rate were recorded before and after buprenorphine administration. RESULTS: We were able to evoke the NWR and temporal summation in foals using this model. Buprenorphine decreased the root mean square amplitude following single electrical stimulation (p < 0.001) in both age groups, and increased the NWR threshold following single electrical stimulation in 2 day old foals (p = 0.0012). Repeated electrical stimulation at 2 Hz was more effective to elicit temporal summation compared to 5 Hz (p < 0.001). No effect of age upon the NWR threshold was found (p = 0.34). Sedation when left undisturbed (11 occasions), increased locomotor activity when handled (9 occasions) and tachypnea (13 occasions) were common side-effects of buprenorphine. CONCLUSION AND CLINICAL RELEVANCE: These findings indicate that buprenorphine has antinociceptive effect in foals. Opioid side effects often recognized in adult horses also occur in foals.

From wires to waves, a novel sensor system for in vivo pressure monitoring.

Pressure monitoring in various organs of the body is essential for appropriate diagnostic and therapeutic purposes. In almost all situations, monitoring is performed in a hospital setting. Technological advances not only promise to improve clinical pressure monitoring systems, but also engage toward the development of fully implantable systems in ambulatory patients. Such systems would not only provide longitudinal time monitoring to healthcare personnel, but also to the patient who could adjust their way-of-life in response to the measurements. In the past years, we have developed a new type of piezoresistive pressure sensor system. Different bench tests have demonstrated that it delivers precise and reliable pressure measurements in real-time. The potential of this system was confirmed by a continuous recording in a patient that lasted for almost a day. In the present study, we further characterized the functionality of this sensor system by conducting in vivo implantation experiments in nine female farm pigs. To get a step closer to a fully implantable system, we also adapted two different wireless communication solutions to the sensor system. The communication protocols are based on MICS (Medical Implant Communication System) and BLE (Bluetooth Low Energy) communication. As a proof-of-concept, implantation experiments in nine female pigs demonstrated the functionality of both systems, with a notable technical superiority of the BLE.

The influence of a continuous rate infusion of dexmedetomidine on the nociceptive withdrawal reflex and temporal summation during isoflurane anaesthesia in dogs.

OBJECTIVE: To examine the influence of a low dose dexmedetomidine infusion on the nociceptive withdrawal reflex and temporal summation in dogs during isoflurane anaesthesia. STUDY DESIGN: Prospective experimental blinded cross-over study. ANIMALS: Eight healthy mixed breed dogs, body weight Mean ± SD 26.5 ± 8.4 kg and age 25 ± 16 months. METHODS: Anaesthesia was induced with propofol and maintained with isoflurane (Fe'ISO 1.3%) delivered in oxygen and air. After stabilization, baseline recordings (time 0) were obtained, then a dexmedetomidine bolus (1 µg kg(-1) IV) followed by a continuous rate infusion (1 µg kg(-1) hour(-1)) or saline placebo were administered. At times 10, 30 and 60 minutes after the initial bolus, electrical stimulations of increasing intensity were applied over the lateral plantar digital nerve, and administered both as single and as repeated stimuli. The resulting reflex responses were recorded using electromyography. Data were analysed using a multivariable linear regression model and a Kruskal Wallis test for single stimulation data, and repeated measures anova and paired t-test for repeated stimulation data. RESULTS: The AUC for the stimulus-response curves after single stimulation were similar for both treatments at time 0. At times 10, 30 and 60 the AUCs for the stimulus-response curves were significantly lower with dexmedetomidine treatment than with placebo. Temporal summation was evident in both treatments at times 0, 10, 30 and 60 starting from a stimulation intensity of 10 mA. The magnitude of temporal summation was smaller in dexmedetomidine than in placebo treated dogs at time 10, 30 and 60, but not at time 0. CONCLUSIONS: During isoflurane anaesthesia, low dose dexmedetomidine suppresses the nociceptive reflex responses after single and repeated electrical stimulation. CLINICAL RELEVANCE: This experimental study confirms previous reports on its peri-operative efficacy under clinical conditions, and further indicates that dexmedetomidine might reduce the risk of post-operative chronic pain development.

Plasma lactate concentrations in free-ranging moose (Alces alces) immobilized with etorphine.

OBJECTIVE: To investigate plasma lactate concentrations of etorphine-immobilized moose in relation to environmental, temporal and physiological parameters. STUDY DESIGN: Prospective clinical study. ANIMALS: Fourteen female and five male moose (Alces alces), estimated age range 1-7 years. METHODS: The moose were darted from a helicopter with 7.5 mg etorphine per animal using projectile syringes and a dart gun. Once immobilized, the moose were approached, a venous blood sample was obtained and vital signs including pulse oximetry were recorded. Diprenorphine was administered to reverse the effects of etorphine. Timing of events, ambient temperature and snow depth were recorded. Blood samples were cooled and centrifuged before plasma was harvested and frozen. The plasma was thawed later and lactate analysed. Data were analysed using descriptive statistics and regression analysis. RESULTS: All animals recovered uneventfully and were alive 12 weeks after immobilization. Mean +/- SD plasma lactate was found to be 9.2 +/- 2.1 mmol L(-1). Plasma lactate concentrations were related positively to snow depth and negatively to time from induction of immobilization to blood sampling. The model that best described the variability in plasma lactate concentrations used induction time (time from firing the dart to the moose being immobilized). The second best model included induction time and snow depth. CONCLUSIONS AND CLINICAL RELEVANCE: Plasma lactate concentrations in these etorphine-immobilized moose were in the range reported for other immobilized wild ruminants. Decreasing induction time, which may be related to a more profound etorphine effect, and increasing snow depth possibly may increase plasma lactate concentrations in etorphine-immobilized moose.

Development of a Multimodal Apparatus to Generate Biomechanically Reproducible Spinal Cord Injuries in Large Animals.

Rodents are widespread animal models in spinal cord injury (SCI) research. They have contributed to obtaining important information. However, some treatments only tested in rodents did not prove efficient in clinical trials. This is probably a result of significant differences in the physiology, anatomy, and complexity between humans and rodents. To bridge this gap in a better way, a few research groups use pig models for SCI. Here we report the development of an apparatus to perform biomechanically reproducible SCI in large animals, including pigs. We present the iterative process of engineering, starting with a weight-drop system to ultimately produce a spring-load impactor. This device allows a graded combination of a contusion and a compression injury. We further engineered a device to entrap the spinal cord and prevent it from escaping at the moment of the impact. In addition, it provides identical resistance around the cord, thereby, optimizing the inter-animal reproducibility. We also present other tools to straighten the vertebral column and to ease the surgery. Sensors mounted on the impactor provide information to assess the inter-animal reproducibility of the impacts. Further evaluation of the injury strength using neurophysiological recordings, MRI scans, and histology shows consistency between impacts. We conclude that this apparatus provides biomechanically reproducible spinal cord injuries in pigs.

Effect of intratesticular injection of lidocaine on cardiovascular responses to castration in isoflurane-anesthetized stallions.

OBJECTIVE: To evaluate the effect of intratesticular administration of lidocaine on cardiovascular responses and cremaster muscle tension during castration of isoflurane-anesthetized stallions. ANIMALS: 28 healthy stallions (mean +/- SD age, 4.2 +/- 2.8 years) with no testicular abnormalities that were scheduled for castration. PROCEDURE: Each horse was given acepromazine (20 microg/kg, IM), romifidine (50 microg/kg, IV), and butorphanol (20 microg/kg, IV). Anesthesia was induced with ketamine (2.5 mg/kg, IV) and midazolam (50 microg/kg, IV) and maintained with isoflurane (1.7% end-tidal concentration). After 10 minutes at a stable anesthetic plane, a needle was placed in each testicle and either no fluid or 15 mL of 2% lidocaine was injected; 10 minutes after needle placement, surgery was commenced. Pulse rate and arterial blood pressures were measured invasively at intervals from 5 minutes prior to castration (baseline) until 5 minutes after the left spermatic cord was clamped. The surgeon subjectively scored the degree of cremaster muscle tension. In 2 horses, lidocaine labeled with radioactive carbon (C(14)) was used and testicular autoradiograms were obtained. RESULTS: Compared with baseline values, castration significantly increased blood pressure measurements; intratesticular injection of lidocaine decreased this blood pressure response and cremaster muscle tension. In 2 horses, autoradiography revealed diffuse distribution of lidocaine into the spermatic cord but poor distribution into the cremaster muscle. CONCLUSIONS AND CLINICAL RELEVANCE: In isoflurane-anesthetized stallions, intratesticular injection of lidocaine prior to castration appeared to decrease intraoperative blood pressure responses and cremaster muscle tension and may be a beneficial supplement to isoflurane anesthesia.

Motor responses to stimulation during isoflurane anaesthesia in pigs.

OBJECTIVE: To investigate motor responses to stimulation during the transition from 'deep' (burst suppression) to 'light' isoflurane anaesthesia in pigs. STUDY DESIGN: Prospective, randomized observational study. ANIMALS: Five castrated male and five female Norwegian landrace pigs, weighing 19-29 kg. MATERIALS AND METHODS: Anaesthesia was induced with isoflurane and the inspired concentration gradually increased until a burst suppression electroencephalogram (EEG) was recorded. End-tidal isoflurane concentration (FE'ISO) was then allowed to equilibrate for 30 minutes after which the eyelashes, cornea, nasal septum, anus, interdigital skin fold, periople, tail and claw were stimulated. The motor response to stimulation at each location was graded from 0 to 5. End-tidal isoflurane concentration was decreased 0.3% and the areas re-stimulated; this was repeated three times in each pig. A linear regression analysis using response as dependent and anaesthetic level as independent variable was performed for each stimulus in each pig. Using Student's t-statistic a 95% confidence interval for the mean slope of each stimulus was constructed. RESULTS: No pig responded to eyelash brushing. The mean slopes for the other stimuli indicated increasing responses with decreasing FE'ISO. Responses to periople pinching and tail and claw clamping showed significant increases. No stimuli consistently increased the magnitude of response in all pigs, and the appearance and absence of a response was inconsistent between pigs. Motor responses occurred in at least one pig during isoflurane burst suppression anaesthesia to all stimuli except eyelash brushing. CONCLUSIONS: All the stimuli investigated may elicit movement responses during burst suppression anaesthesia with isoflurane except eyelash brushing. No consistent response pattern between pigs was observed with decreasing isoflurane concentration. Of the stimuli evaluated, clamping the tail or claw and pinching the periople appear the most reliable indicators of anaesthetic depth. CLINICAL RELEVANCE: The absence or presence of single reflexes does not accurately reflect the degree of isoflurane-induced cortical depression in individual pigs.

Electroencephalographic and cardiovascular indicators of nociception during isoflurane anaesthesia in pigs.

Objective To evaluate electroencephalographic indices, invasive arterial blood pressure and pulse rate as indicators of nociception during isoflurane anaesthesia in pigs. Animals Ten Norwegian Landrace pigs, five castrated males and five females, weighing between 19 and 29 kg. Materials and methods The pigs were anaesthetized with isoflurane and prepared for the measurement of heart rate (HR), mean arterial pressure (MAP) and the electroencephalogram (EEG). The inspired isoflurane concentration (Fi'ISO) was then adjusted until the suppression ratio (SR) exceeded 20%, after which the corresponding end-expired concentration (Fe'ISO) was maintained for 30 minutes. The effects of noxious stimuli, consisting of pinching (nasal septum, interdigital web, anus, periople) and clamping (tail, claw) on spectral edge frequency 95%, median frequency, alpha/delta ratio, beta/delta ratio, theta/delta ratio, total power, suppression ratio, MAP and HR were recorded. Fe'ISO was then decreased by 0.3% and maintained for 30 minutes after which noxious stimulation was re-applied. This was repeated twice with Fe'ISO reductions of 0.3% being applied each time. Changes in measured variables before and after noxious stimulation were then compared at each of four levels of anaesthesia. Results Noxious stimulation caused significant MAP increases at the lowest two levels for Fe'ISO. There were no significant changes in the other variables examined. Conclusion Of the variables examined, mean arterial blood pressure is the most sensitive indicator of nociception in isoflurane-anaesthetized pigs. Clinical relevance When evaluating nociception under isoflurane anaesthesia in pigs mean arterial blood pressure should be monitored when surgery is performed.

Improvement of arterial oxygenation in free-ranging moose (Alces alces) immobilized with etorphine-acepromazine-xylazine.

BACKGROUND: The effect of intranasal oxygen and/or early reversal of xylazine with atipamezole on arterial oxygenation in free-ranging moose (Alces alces) immobilized with etorphine-acepromazine-xylazine with a cross-sectional clinical study on 33 adult moose was evaluated. RESULTS: Before treatment the mean±SD (range) partial pressure of arterial oxygen (PaO2) was 62±17 (26-99) mmHg. Twenty-six animals had a PaO2<80 mmHg. Ten had a PaO2 of 40-60 mmHg and three animals had a PaO2<40 mmHg. Intranasal oxygen and intravenous administration of atipamezole significantly increased the mean PaO2, as did the combination of the two. In contrast, atipamezole administered intramuscularly at the evaluated dose had no significant effect on arterial oxygenation. CONCLUSIONS: This study shows that intranasal oxygen effectively improved arterial oxygenation in immobilized moose, and that early intravenous reversal of the sedative component, in this case xylazine, in an opioid-based immobilization drug-protocol significantly improves arterial oxygenation.

Effect of topical vapocoolant spray on response to arthrocentesis and intravenous catheterization in unsedated horses.

OBJECTIVE: To assess the efficacy of a commercially available topical vapocoolant spray in reducing responses to arthrocentesis of the middle carpal (MC) and metacarpophalangeal (MCP) joints and jugular vein catheterization in unsedated horses. ANIMALS: 8 healthy research horses. PROCEDURES: Arthrocentesis of both MC and MCP joints and bilateral jugular vein catheterization were performed in each horse. Immediately prior to skin penetration, 1 randomly selected MC joint, MCP joint, and jugular vein were sprayed with a vapocoolant liquid (intervention product), and the contralateral MC joint, MCP joint, and jugular vein were sprayed with water (placebo). An observer blinded to the type of spray treatment used evaluated the horses' responses to needle or catheter placement procedures by use of a 6-point categorical scale and a 100-mm visual analog scale. RESULTS: Responses evaluated via the visual analog scale were significantly reduced after application of the intervention product, compared with responses after application of the placebo, for the MC and MCP joints; no difference in responses to jugular vein catheterization was detected between the 2 treatments. CONCLUSIONS AND CLINICAL RELEVANCE: Vapocoolant spray was safe and effective in reducing horses' responses to arthrocentesis. The use of such products prior to joint injections may reduce procedural nociception and pain anticipation in unsedated horses and may improve the safety of personnel performing such procedures.

Effects of isoflurane upon minimum alveolar concentration and cerebral cortex depression in pigs and goats: an interspecies comparison.

This study compared the effects of isoflurane in pigs (n=10 Yorkshire-Landrace cross) and dairy goats (n=10) by evaluation of electroencephalographic (EEG) burst suppression thresholds (BST) in the cerebral cortex and minimum alveolar concentration (MAC) values in the spinal cord. The study also investigated whether individual MAC values can predict the effects of isoflurane on the cerebral cortex. MAC values and BST/MAC ratios were significantly different between species. Inhibition of movement by isoflurane may be less effective in pigs than in goats. No significant correlation was found between individual MAC and BST values, indicating that in single animals the individual MAC poorly reflects the cerebrocortical depressant effect of isoflurane in pigs and goats.

Abnormal motor activity during anaesthesia in a dog: a case report.

Seizures or convulsions that occur during anaesthesia in veterinary patients are infrequently reported in the literature. Consequently, the incidence of such events is unknown. Several drugs commonly used in clinical veterinary anaesthesia have been shown to induce epileptiform activity in both human clinical patients and experimental candidates. The present case report describes convulsions in a four-year old male Bernese mountain dog during maintenance of anaesthesia with isoflurane after premedication with acepromazine and methadone followed by co-induction with propofol and ketamine. The dog had no history of previous convulsions. The use of several sedative and anaesthetic drugs makes it difficult to find one single causative pharmaceutical.

Gastrointestinal stromal tumour and hypoglycemia in a Fjord pony: case report.

BACKGROUND: Neoplasia may cause hypoglycemia in different species including the horse, but hypoglycemia has not previously been reported in the horse associated with gastrointestinal stromal tumours. CASE PRESENTATION: A case of a gastrointestinal stromal tumour in a Fjord pony with severe recurrent hypoglycemia is presented. The mechanism causing the hypoglycemia was not established. CONCLUSION: This case indicates that a gastrointestinal stromal tumour may cause hypoglycemia also in the horse.

Electroencephalographic and cardiovascular variables as nociceptive indicators in isoflurane-anaesthetized horses.

OBJECTIVE: To evaluate Fourier-transformed electroencephalographic (EEG) variables, mean arterial blood pressure (MAP) and pulse rate as nociceptive indicators in isoflurane-anaesthetized horses. ANIMALS: Five standardbred and three Norwegian cold-blooded trotter stallions undergoing castration, aged 2-4 years, mass 378-538 kg. MATERIALS AND METHODS: All horses received intravenous (IV) detomidine (10 microg kg(-1) IV) and butorphanol (0.01 mg kg(-1) IV). Additional detomidine (4 microg kg(-1) IV) was administered in the induction area. Anaesthesia was induced with ketamine (2.5 mg kg(-1) IV) and diazepam (40 microg kg(-1) IV), and maintained for 30 minutes with isoflurane (end-tidal concentration of 1.4%) vaporized in oxygen. The electroencephalogram, MAP and pulse rate were recorded for 15 minutes, beginning 5 minutes before skin incision. Differences between the mean values of recordings taken before, and during surgery were calculated and tested for significant differences using a two-sided Student's t-test. RESULTS: A significant rise in MAP and a fall in pulse rate were found. No significant change was found in any EEG variable. CONCLUSION/CLINICAL relevance Of the variables evaluated, MAP seems to be the most sensitive and reliable indicator of nociception in isoflurane-anaesthetized horses.

Castration of piglets: the analgesic effects of intratesticular and intrafunicular lidocaine injection.

OBJECTIVE: The aim of this study was to evaluate the analgesic effect of intratesticular and intrafunicular lidocaine for the surgical castration of piglets and to investigate the degree of nociception induced by lidocaine injection. STUDY DESIGN: Prospective controlled experimental study. ANIMALS: Forty-seven male Norwegian landrace piglets with normal testicular anatomy, aged 22 (+/-2.6 SD) days and weighing 7.4 +/- 1.4 kg. MATERIALS AND METHODS: Anaesthesia was induced and maintained using halothane delivered in oxygen. End-tidal halothane was stabilized at 1.3% for 20 minutes before mean arterial blood pressure (MAP) pulse rate and electroencephalography (EEG) monitoring began. After 5 minutes of data collection, scrotal skin was desensitized with lidocaine before either an intrafunicular (IF) (n = 15) or an intratesticular (IT) (n = 16) lidocaine injection was made. Pigs in the control group (n = 16) did not receive lidocaine. Ten minutes later, a scalpel and an emasculator were used to cut the funiculus spermaticus. The MAP, pulse rate and EEG were monitored continuously for 5 minutes after castration. RESULTS: During castration, MAP increased significantly, while pulse rate and EEG theta power fell significantly more in control, compared with the IT or IF groups. EEG alpha power fell more in the control group than in the IF group. No significant differences were found between the IF and IT groups. EEG, MAP and pulse rate responses to castration in the control group were significantly larger than the response to lidocaine injection. CONCLUSION/CLINICAL RELEVANCE: Injecting lidocaine into the funiculus spermaticus or into the testes is effective in reducing signs of nociception caused by castration. Lidocaine injection is less noxious than castration without local anaesthetic.