RESUMO
The effect of acetylsalicylic acid (aspirin) on the ultrastructure of gastric and jejunal mucosa was investigated in patients undergoing gastric surgery and in guinea pigs. The drug caused various degrees of damage to the surface mucous cells in both species perceptible as general signs of cytolysis in situ or as desquanmation. In patients pretreated with aspirin, an increase of secondary lysosomes was noted in the gastric parietal cells but not in the animals. In the intestinal epithelial cells of both species there was a marked increase of multivesicular bodies and the occurrence of transitional stages between the two organoids suggest a functional interrelationship. Since no specific alteration of any cellular organoid was detected, the drug-induced injury is assumed to occur on a molecular level in the cytoplasm. It is concluded that the intracellular concentrations of aspirin in gastrointestinal mucosal cells mechanims but that high drug concentrations may lead to irreversible cell damage.
Assuntos
Aspirina/farmacologia , Mucosa Gástrica/ultraestrutura , Mucosa Intestinal/ultraestrutura , Animais , Grânulos Citoplasmáticos/efeitos dos fármacos , Mucosa Gástrica/efeitos dos fármacos , Cobaias , Humanos , Mucosa Intestinal/efeitos dos fármacos , Lisossomos/efeitos dos fármacos , Especificidade da EspécieRESUMO
Data are presented on the acute toxicity (mortality only) of the thermal degradation products of polymers obtained by two methods of degradation. One system utilized a slowly increasing temperature (5 degrees C/min) and gradual degradation of the polymer with the rats being exposed to degradation products as they were evolved. In this system the more toxic polymers included wool, polypropylene, poly(vinyl chloride), and urethane foam. The second system utilized conditions of rapid combustion and exposure of rats to the total products of combustion for a period of 4 hr. In this system the more toxic materials included red oak, cotton, acrylonitrile-butadiene-styrene (ABS), and styrene-acrylonitrile. It is of interest to note that the natural product wool is among the least toxic under these rapid combustion conditions and among the most toxic under slow pyrolysis conditions. Other materials also vary in the comparative toxicity of their thermal degradation products, depending upon the conditions of degradation and animal exposure. The two experimental techniques presented here may well represent the two extreme conditions of rapid combustion versus slow pyrolysis. Intermediate types of fire situations might be expected to result in relative acute toxicities somewhere between these two extremes. This report deals with acute toxicity on the basis of mortality data only and does not include other parameters of toxicity such as organ weights and histopathology.
Assuntos
Polímeros/toxicidade , Animais , Câmaras de Exposição Atmosférica , Exposição Ambiental , Incêndios , Gossypium/toxicidade , Masculino , Nylons/toxicidade , Poliuretanos/toxicidade , Ratos , Tiocianatos/sangue , Fatores de Tempo , Madeira , Lã/toxicidadeRESUMO
Changes in food intake, serum adipsin, and obesity were evaluated in the MSG-treated mouse. In Experiment 1, mice treated with MSG had 50% lower serum adipsin and over 2-fold higher percentage of body fat than the lean controls. Both feeding caffeine and restricting intake normalized serum adipsin and caused weight loss, but did not normalize the percentage of body fat. No additional effect was gained by feeding isoproterenol or ephedrine in combination with caffeine. In Experiment 2, we separated the direct effect of caffeine from the associated depression in intake using a paired feeding design, and also determined the effects of selected adrenergic agents and somatotropin (S). Somatotropin increased weight gain and reduced the percentage of body fat in healthy and obese mice, and tended to lower serum adipsin. Caffeine clearly depressed intake, caused weight loss, and increased serum adipsin, but similar results were achieved by restricting intake. None of the adrenergic drugs tested changed serum adipsin. Ephedrine depressed food intake and caused weight loss, but reduced the percentage of body fat only at the highest dietary concentration (2000 mg per kg of diet). Phenylephrine reduced weight gain without a concomitant effect on the percentage of body fat, and isoproterenol did not influence weight gain or body fat.
Assuntos
Composição Corporal/efeitos dos fármacos , Serina Endopeptidases/biossíntese , Glutamato de Sódio/farmacologia , Agonistas alfa-Adrenérgicos/farmacologia , Agonistas Adrenérgicos beta/farmacologia , Animais , Peso Corporal/efeitos dos fármacos , Cafeína/farmacologia , Estimulantes do Sistema Nervoso Central/farmacologia , Fator D do Complemento , Ingestão de Alimentos/efeitos dos fármacos , Efedrina/farmacologia , Etanolaminas/farmacologia , Feminino , Hormônio do Crescimento/farmacologia , Isoproterenol/farmacologia , Lactação/fisiologia , Camundongos , Fenilefrina/farmacologia , Serina Endopeptidases/sangueRESUMO
Specific difficulties connected with clinical evaluation and especially dose finding for antiarrhythmic substances are discussed. They are mainly due to the differences in pathogenesis, clinical significance and to the inconsistency of the symptom arrhythmia. Therefore special cardiologic skills are required for performance and interpretation of respective studies. The importance of selection of patients and determination of plasma levels of the substance is demonstrated. Possible procedures for determination of the therapeutic dosage are described and the necessity of detailed documentation of side effects is stressed.
Assuntos
Antiarrítmicos/administração & dosagem , Antiarrítmicos/metabolismo , Antiarrítmicos/uso terapêutico , Arritmias Cardíacas/tratamento farmacológico , Avaliação de Medicamentos/métodos , Humanos , CinéticaRESUMO
The antihypertensive efficacy of anipamil, a novel calcium antagonist of the verapamil type, was tested in an open group comparison. One hundred twenty-one hypertensive patients (World Health Organization stage I-II) entered the study in 17 centers in Germany and Austria. After a placebo washout period patients were randomly allocated to either 40, 80, or 120 mg of anipamil for a period of 7 days. This treatment period was followed by another 7-day placebo period. Anipamil lowered systolic and diastolic blood pressure in all three dosages tested. The 80-mg dose was more effective than 40 mg. The 120-mg dose did not show an increased effect over 80 mg. Heart rate was slightly lowered, reaching statistical significance in the groups receiving 80 and 120 mg. In the dosages tested, anipamil had no effect on PQ-interval or other electrocardiogram parameters. Major or clinically relevant changes in the laboratory parameters could not be detected. Side effects were rare, mild, and transient. No patient had to be discharged from the trial due to severe side effects.
Assuntos
Bloqueadores dos Canais de Cálcio/uso terapêutico , Hipertensão/tratamento farmacológico , Propilaminas/uso terapêutico , Adulto , Pressão Sanguínea/efeitos dos fármacos , Bloqueadores dos Canais de Cálcio/administração & dosagem , Eletrocardiografia , Feminino , Frequência Cardíaca/efeitos dos fármacos , Humanos , Hipertensão/fisiopatologia , Masculino , Propilaminas/administração & dosagemRESUMO
A method for monitoring exposure to ethylenediamine (EDA) in the occupational environment is described. The EDA is adsorbed on activated silica gel, desorbed with 0.5 percent aqueous cupric chloride, and analyzed by gas chromatography using a 2 percent KOH on a Chromosorb 103 column. The method is sensitive to 200 micrograms/mL EDA and can detect 1.0 ppmv EDA in samples collected for 4.5 hours at a 300 cc per minute flow. The method has been evaluated in the laboratory and under plant conditions. Other amines do not interfere with the determination of EDA.
Assuntos
Poluentes Ocupacionais do Ar/análise , Poluentes Atmosféricos/análise , Etilenodiaminas/análise , Exposição Ambiental , MétodosRESUMO
To learn how signals from adipocytes might be involved in regulation of energy intake and storage, we have begun to characterize the porcine complement protein, adipsin. Adipsin was originally identified as a protein that is produced rather specifically by adipocytes, is secreted, and is nearly absent in several obese rodent models. We now report that porcine adipsin mRNA sequence is 74% identical to rat and predicts a protein that has 82 and 68% identity to human and rat forms, respectively. Porcine adipsin has none of the asparagine glycosylation consensus sites which make recombinant expression of mouse adipsin in Escherichia coli impractical. We present a method for engineering the porcine cDNA to facilitate expression by E. coli and provide a protocol for refolding and purifying porcine adipsin protein and for immunoassay. We have found that in addition to adipose tissue, adipsin mRNA is present in gut tissues. Coupled with the fact that adipsin is required for processing of complement C3a-desArg, and that C3a-desArg is a potent stimulant of fatty acid acylation in adipocytes, the production of adipsin in the gut may be related to a mechanism for adipocyte removal of lipid from chylomicrons.
Assuntos
Clonagem Molecular/métodos , Fator D do Complemento/metabolismo , Serina Endopeptidases/biossíntese , Animais , Sequência de Bases , Northern Blotting , Escherichia coli/genética , Dados de Sequência Molecular , Dobramento de Proteína , RNA Mensageiro/metabolismo , Radioimunoensaio , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Alinhamento de Sequência , Serina Endopeptidases/genética , Serina Endopeptidases/imunologia , Suínos , Distribuição TecidualRESUMO
This chapter concludes the survey of experimental and clinical data on the analgesic and antitussive properties of codeine and its potential therapeutic alternates. From an evaluation of their effectiveness on the one hand and the side-effects, including tolerance, dependence and abuse liability on the other, it would appear that the therapeutic goals of codeine could be achieved by other substances, except perhaps where analgesia, cough relief, and sedation are required simultaneously. The use of these other substances would, however, result in no particular gain and probably no particular loss.
Assuntos
Antitussígenos/administração & dosagem , Codeína/administração & dosagem , Tosse/tratamento farmacológico , Analgésicos/administração & dosagem , Animais , Antitussígenos/efeitos adversos , Codeína/efeitos adversos , Humanos , Transtornos Relacionados ao Uso de SubstânciasRESUMO
In this report-the fourth of a series on codeine and its alternates for pain and cough relief-an attempt is made to evaluate, on the basis of experimental and clinical data, and wherever possible in comparison with codeine, the effectiveness of a number of antitussive substances currently in clinical use. In the discussion of the undesired side-effects particular attention is paid to the risk of dependence and abuse.
Assuntos
Antitussígenos/administração & dosagem , Codeína/administração & dosagem , Tosse/tratamento farmacológico , Alcaloides/administração & dosagem , Animais , Antitussígenos/efeitos adversos , Gatos , Cães , Cobaias , Antagonistas dos Receptores Histamínicos H1/administração & dosagem , Humanos , Isoquinolinas/administração & dosagem , Meperidina/administração & dosagem , Metadona/administração & dosagem , Camundongos , Morfinanos/administração & dosagem , Fenantrenos/administração & dosagem , Fenotiazinas/administração & dosagem , Ratos , Transtornos Relacionados ao Uso de SubstânciasRESUMO
This report-the third of a series on codeine and its alternates for pain and cough relief-presents a detailed review of the physiology and pathophysiology of cough, the methods for the experimental and clinical measurement of the antitussive action of drugs, possible mechanisms of action of antitussive agents, and includes a compilation of experimental results and clinical experience with codeine as an antitussive.
Assuntos
Antitussígenos , Codeína , Animais , Antitussígenos/administração & dosagem , Antitussígenos/farmacologia , Antitussígenos/normas , Criança , Pré-Escolar , Codeína/normas , Tosse/fisiopatologia , Cães , Humanos , Lactente , Métodos , Camundongos , Dor/tratamento farmacológico , Coelhos , RatosRESUMO
This report-the second of a series on codeine and its alternates for pain and cough relief-contains a detailed evaluation of experimental and clinical data on newer substances having analgesic properties comparable to and in approximately the same range as those of codeine. The data are discussed under the headings: analgesic effects in animals; clinical usefulness; side-effects with particular reference to dependence and abuse liability.
Assuntos
Codeína/uso terapêutico , Tosse/tratamento farmacológico , Dor/tratamento farmacológico , Amidas/uso terapêutico , Analgésicos/uso terapêutico , Animais , Antitussígenos/uso terapêutico , Azepinas/uso terapêutico , Carisoprodol/uso terapêutico , Gatos , Galinhas , Ciclazocina/uso terapêutico , Dextropropoxifeno/uso terapêutico , Ácidos Difenilacéticos/uso terapêutico , Cães , Patos , Cobaias , Humanos , Indenos/uso terapêutico , Indóis/uso terapêutico , Isoquinolinas/uso terapêutico , Camundongos , Morfinanos/uso terapêutico , Nalorfina/uso terapêutico , Antagonistas de Entorpecentes/uso terapêutico , Pentazocina/uso terapêutico , Fenetilaminas/uso terapêutico , Pirrolidinas/uso terapêutico , Ratos , Transtornos Relacionados ao Uso de Substâncias , Talidomida/uso terapêuticoRESUMO
This report-the first of a series on codeine and its alternates for pain and cough relief-presents a detailed evaluation of experimental and clinical data concerning the analgesic action of codeine (the antitussive action will be assessed separately). The authors discuss the pharmacology of the drug, including side-effects and toxicity; effects on the respiratory, circulatory, digestive and urinary systems; tolerance, dependence and liability to abuse; metabolic effects; and mechanism of action.Though codeine is generally more toxic than morphine to animals on account of its convulsant action, it is less toxic to man, possibly because it produces less respiratory depression. Again, tolerance to its analgesic effects has been demonstrated in several animal species, but dependence in man is observed far less frequently than it is with morphine, and the abstinence syndrome is less intense. From their extensive review of the evidence available, the authors conclude that codeine is a good analgesic and that little risk to public health is likely to arise from its clinical use to relieve pain.
Assuntos
Codeína , Tosse/tratamento farmacológico , Analgésicos/farmacologia , Animais , Circulação Sanguínea/efeitos dos fármacos , Gatos , Codeína/efeitos adversos , Codeína/metabolismo , Codeína/intoxicação , Codeína/toxicidade , Sistema Digestório/efeitos dos fármacos , Cães , Enzimas/sangue , Haplorrinos , Humanos , Metabolismo/efeitos dos fármacos , Camundongos , Morfina/farmacologia , Músculo Liso/efeitos dos fármacos , Coelhos , Ratos , Sistema Respiratório/efeitos dos fármacos , Transtornos Relacionados ao Uso de SubstânciasRESUMO
Endothelin (ET) receptor subtypes in human prostate with benign prostatic hyperplasia were investigated by binding and functional studies. In the displacement experiment, LU224332 [endothelin-A/-B (ET(A)/ET(B)) nonselective antagonist] competed for [125I]ET-1 binding with a monophasic curve. On the other hand, LU135252 (ET(A)-selective antagonist) and sarafotoxin S6c (S6c, ET(B)-selective agonist) competed for [125I]ET-1 binding with shallow and biphasic curves. The analysis of the displacement curves for LU135252 and S6c showed that both ET(A) and ET(B) subtypes coexist but that ET(A) is the dominantly expressed receptor. In human prostate strips, 10 microM of both LU135252 and LU224332 strongly inhibited the contractile response to ET-1 with equal potency. However, 10 microM of BQ788 (ET(B)-selective antagonist) did not show a clear inhibition. S6c also produced a contractile response, which was potently inhibited by LU224332 or BQ788, and slightly suppressed by LU135252. These results suggest that in human prostate both ET(A) and ET(B) subtypes are functional receptors mediating contraction, but that ET-1-mediated contractions are predominantly mediated by activation of dominant receptor subtype, ET(A).